Apoptosis of rat gastric mucosa and of primary cultures of gastric epithelial cells by indomethacin: role of inducible nitric oxide synthase and interleukin-8.
| Title: | Apoptosis of rat gastric mucosa and of primary cultures of gastric epithelial cells by indomethacin: role of inducible nitric oxide synthase and interleukin-8. |
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| Authors: | Imamine, Satoshi1, Akbar, SK. MD. fazle1, Mizukami, Yuji1, Matsui, Hidetaka1, Onji, Morikazu1 |
| Source: | International Journal of Experimental Pathology. Aug2001, Vol. 82 Issue 4, p221-229. 9p. 19 Black and White Photographs, 1 Chart, 1 Graph. |
| Subject Terms: | *APOPTOSIS, *INDOMETHACIN, *NITRIC-oxide synthases, *INTERLEUKIN-8 |
| Abstract: | In order to gain insights into indomethacin-induced gastric injury, rats were fed with indomethacin (20 mg/kg), or alternatively, the primary cultures of rat gastric epithelial cells were cultured with different doses of indomethacin (1–1000 µm). Light microscopy, electron mircroscopy, fluorescence microscopy, TdT-mediated dUTP-biotin nick end labelling staining, ssDNA staining and DNA fragmentation assay were employed to evaluate the levels of gastric injury and apoptosis. Cells expressing inducible nitric oxide synthase (iNOS) and interleukin (IL)-8 were localized at the rat gastric mucosa by immunohistochemistry. Administration of indomethacin to rats caused apoptosis and injury of the gastric mucosal epithelial cells. Indomethacin also induced apoptosis of primary cultures of gastric epithelial cells in a dose-dependent manner. Cells expressing iNOS and IL-8 were detected at and around the sites of gastric injury in the indomethacin-fed rats, but not in the control rats. The induction of apoptosis by indomethacin in the primary cultures of gastric epithelial cells suggests that the direct apoptotic capacity of indomethacin. iNOS and IL-8 may be involved in this process. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Apoptosis of rat gastric mucosa and of primary cultures of gastric epithelial cells by indomethacin: role of inducible nitric oxide synthase and interleukin-8. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Imamine%2C+Satoshi%22">Imamine, Satoshi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Akbar%2C+SK%2E+MD%2E+fazle%22">Akbar, SK. MD. fazle</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mizukami%2C+Yuji%22">Mizukami, Yuji</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Matsui%2C+Hidetaka%22">Matsui, Hidetaka</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Onji%2C+Morikazu%22">Onji, Morikazu</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Experimental+Pathology%22">International Journal of Experimental Pathology</searchLink>. Aug2001, Vol. 82 Issue 4, p221-229. 9p. 19 Black and White Photographs, 1 Chart, 1 Graph. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22APOPTOSIS%22">APOPTOSIS</searchLink><br />*<searchLink fieldCode="DE" term="%22INDOMETHACIN%22">INDOMETHACIN</searchLink><br />*<searchLink fieldCode="DE" term="%22NITRIC-oxide+synthases%22">NITRIC-oxide synthases</searchLink><br />*<searchLink fieldCode="DE" term="%22INTERLEUKIN-8%22">INTERLEUKIN-8</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In order to gain insights into indomethacin-induced gastric injury, rats were fed with indomethacin (20 mg/kg), or alternatively, the primary cultures of rat gastric epithelial cells were cultured with different doses of indomethacin (1–1000 µm). Light microscopy, electron mircroscopy, fluorescence microscopy, TdT-mediated dUTP-biotin nick end labelling staining, ssDNA staining and DNA fragmentation assay were employed to evaluate the levels of gastric injury and apoptosis. Cells expressing inducible nitric oxide synthase (iNOS) and interleukin (IL)-8 were localized at the rat gastric mucosa by immunohistochemistry. Administration of indomethacin to rats caused apoptosis and injury of the gastric mucosal epithelial cells. Indomethacin also induced apoptosis of primary cultures of gastric epithelial cells in a dose-dependent manner. Cells expressing iNOS and IL-8 were detected at and around the sites of gastric injury in the indomethacin-fed rats, but not in the control rats. The induction of apoptosis by indomethacin in the primary cultures of gastric epithelial cells suggests that the direct apoptotic capacity of indomethacin. iNOS and IL-8 may be involved in this process. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of International Journal of Experimental Pathology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1365-2613.2001.00189.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 221 Subjects: – SubjectFull: APOPTOSIS Type: general – SubjectFull: INDOMETHACIN Type: general – SubjectFull: NITRIC-oxide synthases Type: general – SubjectFull: INTERLEUKIN-8 Type: general Titles: – TitleFull: Apoptosis of rat gastric mucosa and of primary cultures of gastric epithelial cells by indomethacin: role of inducible nitric oxide synthase and interleukin-8. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Imamine, Satoshi – PersonEntity: Name: NameFull: Akbar, SK. MD. fazle – PersonEntity: Name: NameFull: Mizukami, Yuji – PersonEntity: Name: NameFull: Matsui, Hidetaka – PersonEntity: Name: NameFull: Onji, Morikazu IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2001 Type: published Y: 2001 Identifiers: – Type: issn-print Value: 09599673 Numbering: – Type: volume Value: 82 – Type: issue Value: 4 Titles: – TitleFull: International Journal of Experimental Pathology Type: main |
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