Bibliographic Details
| Title: |
Nonintegrating Lentiviral Vectors Can Effectively Deliver Ovalbumin Antigen for Induction of Antitumor Immunity. |
| Authors: |
Biliang Hu, Haiguang Yang, Bingbing Dai, April Tai, Pin Wang |
| Source: |
Human Gene Therapy. Dec2009, Vol. 20 Issue 12, p1652-1664. 13p. |
| Subject Terms: |
*LENTIVIRUS diseases, *ALBUMINS, *ANTIGENS, *ANTINEOPLASTIC agents, *TRANSGENE expression, *VIRAL genetics, *IMMUNODEFICIENCY, *CANCER treatment, *GENE therapy, *THERAPEUTICS |
| Abstract: |
AbstractIt has been demonstrated that nonintegrating lentiviral vectors (NILVs) are efficient in maintaining transgene expression in vitroand in vivo. Gene delivery by NILVs can significantly reduce nonspecific vector integration, which has been shown to cause malignant transformation in patients receiving gene therapy for X-linked severe combined immunodeficiency. Strong and sustained immune responses were observed after a single immunization with NILVs carrying viral antigens. However, there is no report to date that evaluates the efficacy of NILVs in inducing antigen-specific antitumor immunity. Using a well-characterized tumor model, we tested in vivoimmunization with a self-inactivating lentiviral vector harboring a defective integrase. A high frequency of ovalbumin peptide (OVAp1)-specific CD8+T cells and a substantial antibody response were detected in naive mice immunized with an NILV encoding an OVA transgene. Furthermore, this immunization method completely protected the mice against the growth of E.G7 tumor cells expressing the OVA antigen. Thus, this study provides evidence that immunization using NILVs can be a safe and promising approach for exploring cancer immunotherapy. [ABSTRACT FROM AUTHOR] |
|
Copyright of Human Gene Therapy is the property of Mary Ann Liebert, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
