Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death.
| Title: | Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death. |
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| Authors: | Ma, Yee Hang Ethan1 (AUTHOR), Putta, Abhinay R.2 (AUTHOR), Chan, Cyrus H. H.2,3 (AUTHOR), Vidman, Stephen R.3,4 (AUTHOR), Monje, Paula1,4 (AUTHOR), Plant, Giles W.1,2 (AUTHOR) giles.plant@osumc.edu |
| Source: | Cells (2073-4409). Mar2025, Vol. 14 Issue 6, p461. 24p. |
| Subject Terms: | *SCHWANN cells, *SERUM-free culture media, *FACIAL transplantation, *CELL death, *SPINAL cord injuries |
| Abstract: | Schwann cell (SC) transplantation shows promise in treating spinal cord injury as a pro-regenerative agent to allow host endogenous neurons to bridge over the lesion. However, SC transplants face significant oxidative stress facilitated by ROS in the lesion, leading to poor survival. deferoxamine mesylate (DFO) is a neuroprotective agent shown to reduce H2O2-induced cell death in serum-containing conditions. Here we show that DFO is not necessary to induce neuroprotection under serum-free conditions by cell survival quantification and phenotypic analysis via immunohistochemistry, Hif1α and collagen IV quantification via whole cell corrected total cell fluorescence, and cell death transcript changes via RT-qPCR. Our results indicate survival of SC regardless of DFO pretreatment in serum-free conditions and an increased survival facilitated by DFO in serum-containing conditions. Furthermore, our results showed strong nuclear expression of Hif1α in serum-free conditions regardless of DFO pre-treatment and a nuclear expression of Hif1α in DFO-treated SCs in serum conditions. Transcriptomic analysis reveals upregulation of autophagy transcripts in SCs grown in serum-free media relative to SCs in serum conditions, with and without DFO and H2O2. Thus, indicating a pro-repair and regenerative state of the SCs in serum-free conditions. Overall, results indicate the protectiveness of CDM in enhancing SC survival against ROS-induced cell death in vitro. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ma%2C+Yee+Hang+Ethan%22">Ma, Yee Hang Ethan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Putta%2C+Abhinay+R%2E%22">Putta, Abhinay R.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chan%2C+Cyrus+H%2E+H%2E%22">Chan, Cyrus H. H.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vidman%2C+Stephen+R%2E%22">Vidman, Stephen R.</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Monje%2C+Paula%22">Monje, Paula</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Plant%2C+Giles+W%2E%22">Plant, Giles W.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> giles.plant@osumc.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Cells+%282073-4409%29%22">Cells (2073-4409)</searchLink>. Mar2025, Vol. 14 Issue 6, p461. 24p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22SCHWANN+cells%22">SCHWANN cells</searchLink><br />*<searchLink fieldCode="DE" term="%22SERUM-free+culture+media%22">SERUM-free culture media</searchLink><br />*<searchLink fieldCode="DE" term="%22FACIAL+transplantation%22">FACIAL transplantation</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+death%22">CELL death</searchLink><br />*<searchLink fieldCode="DE" term="%22SPINAL+cord+injuries%22">SPINAL cord injuries</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Schwann cell (SC) transplantation shows promise in treating spinal cord injury as a pro-regenerative agent to allow host endogenous neurons to bridge over the lesion. However, SC transplants face significant oxidative stress facilitated by ROS in the lesion, leading to poor survival. deferoxamine mesylate (DFO) is a neuroprotective agent shown to reduce H2O2-induced cell death in serum-containing conditions. Here we show that DFO is not necessary to induce neuroprotection under serum-free conditions by cell survival quantification and phenotypic analysis via immunohistochemistry, Hif1α and collagen IV quantification via whole cell corrected total cell fluorescence, and cell death transcript changes via RT-qPCR. Our results indicate survival of SC regardless of DFO pretreatment in serum-free conditions and an increased survival facilitated by DFO in serum-containing conditions. Furthermore, our results showed strong nuclear expression of Hif1α in serum-free conditions regardless of DFO pre-treatment and a nuclear expression of Hif1α in DFO-treated SCs in serum conditions. Transcriptomic analysis reveals upregulation of autophagy transcripts in SCs grown in serum-free media relative to SCs in serum conditions, with and without DFO and H2O2. Thus, indicating a pro-repair and regenerative state of the SCs in serum-free conditions. Overall, results indicate the protectiveness of CDM in enhancing SC survival against ROS-induced cell death in vitro. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Cells (2073-4409) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/cells14060461 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 24 StartPage: 461 Subjects: – SubjectFull: SCHWANN cells Type: general – SubjectFull: SERUM-free culture media Type: general – SubjectFull: FACIAL transplantation Type: general – SubjectFull: CELL death Type: general – SubjectFull: SPINAL cord injuries Type: general Titles: – TitleFull: Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ma, Yee Hang Ethan – PersonEntity: Name: NameFull: Putta, Abhinay R. – PersonEntity: Name: NameFull: Chan, Cyrus H. H. – PersonEntity: Name: NameFull: Vidman, Stephen R. – PersonEntity: Name: NameFull: Monje, Paula – PersonEntity: Name: NameFull: Plant, Giles W. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 03 Text: Mar2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 20734409 Numbering: – Type: volume Value: 14 – Type: issue Value: 6 Titles: – TitleFull: Cells (2073-4409) Type: main |
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