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Esculin promotes skin wound healing in mice and regulates the Wnt/β-catenin signaling pathway.

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Title: Esculin promotes skin wound healing in mice and regulates the Wnt/β-catenin signaling pathway.
Authors: Xu, Mian1 (AUTHOR), Zhang, Mengsi2 (AUTHOR) zms123098@163.com, Wu, Jingjing1 (AUTHOR), Wang, Jinmeng1 (AUTHOR), Wu, Huaze1 (AUTHOR), Xu, Xianting1 (AUTHOR)
Source: CytoJournal. 3/12/2025, Vol. 22, p1-10. 10p.
Subject Terms: *SKIN injuries, *WOUND healing, *BIOLOGICAL models, *PHOSPHORYLATION, *CELL proliferation, *CELLULAR signal transduction, *CYTOSKELETAL proteins, *TREATMENT effectiveness, *FLUORESCENT antibody technique, *MICE, *IMMUNOHISTOCHEMISTRY, *GLYCOSIDES, *ANIMAL experimentation, *WESTERN immunoblotting, *BENZOPYRANS, *COMPARATIVE studies
Abstract: Objective Previous studies reported that esculin could protect against renal ischemia-reperfusion injury and liver injury, but its mechanism of action in skin wound healing is unclear. The Wnt/β-catenin signaling pathway plays a positive role in the wound healing process. This study aimed to investigate the effects of esculin on the rate and quality of skin wound healing in mice and explore its regulatory role in the Wnt/b-catenin signaling pathway. Material and Methods Circular full-thickness skin wounds with a diameter of 8 mm were created on the backs of C57BL/6 mice, which were administered with 20 and 40 mg•kg−1 esculin through gastric lavage. Wound healing was monitored, and samples collected on day 14 were analyzed through hematoxylin-eosin and Masson staining to assess granulation tissue formation and collagen deposition. Immunohistochemistry, immunofluorescence, and Western blot evaluated markers of collagen synthesis, proliferation, angiogenesis, and proteins in the Wnt/b-catenin signaling pathway. National institutes of health/3T3 cells treated with esculin (50 and 200 μM) were analyzed for proliferating cell nuclear antigen (PCNA) expression to assess proliferative activity. Results Compared with the model group, the esculin-treated groups exhibited significantly enhanced wound healing (P < 0.05), increased skin epithelial thickness (P < 0.01), and promoted extracellular matrix formation in mice. In addition, esculin significantly raised type I collagen alpha-1 chain and type III collagen alpha-1 chain protein levels (P < 0.05), boosted the expression of the cell proliferation marker PCNA and the vascular marker cluster of differentiation 31 in the dermis (P < 0.05), and upregulated proteins related to the Wnt/b-catenin signaling pathway and increased glycogen synthase kinase 3 beta phosphorylation in skin wound and NIH/3T3 cells (P < 0.05). Conclusion Esculin could upregulate and activate the Wnt/b-catenin signaling pathway to promote wound healing. [ABSTRACT FROM AUTHOR]
Copyright of CytoJournal is the property of Scientific Scholar LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Esculin promotes skin wound healing in mice and regulates the Wnt/β-catenin signaling pathway.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Xu%2C+Mian%22&quot;&gt;Xu, Mian&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhang%2C+Mengsi%22&quot;&gt;Zhang, Mengsi&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; zms123098@163.com&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wu%2C+Jingjing%22&quot;&gt;Wu, Jingjing&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang%2C+Jinmeng%22&quot;&gt;Wang, Jinmeng&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wu%2C+Huaze%22&quot;&gt;Wu, Huaze&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Xu%2C+Xianting%22&quot;&gt;Xu, Xianting&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22CytoJournal%22&quot;&gt;CytoJournal&lt;/searchLink&gt;. 3/12/2025, Vol. 22, p1-10. 10p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22SKIN+injuries%22&quot;&gt;SKIN injuries&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22WOUND+healing%22&quot;&gt;WOUND healing&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22BIOLOGICAL+models%22&quot;&gt;BIOLOGICAL models&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22PHOSPHORYLATION%22&quot;&gt;PHOSPHORYLATION&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22CELL+proliferation%22&quot;&gt;CELL proliferation&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22CELLULAR+signal+transduction%22&quot;&gt;CELLULAR signal transduction&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22CYTOSKELETAL+proteins%22&quot;&gt;CYTOSKELETAL proteins&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22TREATMENT+effectiveness%22&quot;&gt;TREATMENT effectiveness&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22FLUORESCENT+antibody+technique%22&quot;&gt;FLUORESCENT antibody technique&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22MICE%22&quot;&gt;MICE&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22IMMUNOHISTOCHEMISTRY%22&quot;&gt;IMMUNOHISTOCHEMISTRY&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22GLYCOSIDES%22&quot;&gt;GLYCOSIDES&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22ANIMAL+experimentation%22&quot;&gt;ANIMAL experimentation&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22WESTERN+immunoblotting%22&quot;&gt;WESTERN immunoblotting&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22BENZOPYRANS%22&quot;&gt;BENZOPYRANS&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22COMPARATIVE+studies%22&quot;&gt;COMPARATIVE studies&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Objective Previous studies reported that esculin could protect against renal ischemia-reperfusion injury and liver injury, but its mechanism of action in skin wound healing is unclear. The Wnt/β-catenin signaling pathway plays a positive role in the wound healing process. This study aimed to investigate the effects of esculin on the rate and quality of skin wound healing in mice and explore its regulatory role in the Wnt/b-catenin signaling pathway. Material and Methods Circular full-thickness skin wounds with a diameter of 8 mm were created on the backs of C57BL/6 mice, which were administered with 20 and 40 mg•kg−1 esculin through gastric lavage. Wound healing was monitored, and samples collected on day 14 were analyzed through hematoxylin-eosin and Masson staining to assess granulation tissue formation and collagen deposition. Immunohistochemistry, immunofluorescence, and Western blot evaluated markers of collagen synthesis, proliferation, angiogenesis, and proteins in the Wnt/b-catenin signaling pathway. National institutes of health/3T3 cells treated with esculin (50 and 200 μM) were analyzed for proliferating cell nuclear antigen (PCNA) expression to assess proliferative activity. Results Compared with the model group, the esculin-treated groups exhibited significantly enhanced wound healing (P &lt; 0.05), increased skin epithelial thickness (P &lt; 0.01), and promoted extracellular matrix formation in mice. In addition, esculin significantly raised type I collagen alpha-1 chain and type III collagen alpha-1 chain protein levels (P &lt; 0.05), boosted the expression of the cell proliferation marker PCNA and the vascular marker cluster of differentiation 31 in the dermis (P &lt; 0.05), and upregulated proteins related to the Wnt/b-catenin signaling pathway and increased glycogen synthase kinase 3 beta phosphorylation in skin wound and NIH/3T3 cells (P &lt; 0.05). Conclusion Esculin could upregulate and activate the Wnt/b-catenin signaling pathway to promote wound healing. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of CytoJournal is the property of Scientific Scholar LLC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder&#39;s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.25259/Cytojournal_184_2024
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 1
    Subjects:
      – SubjectFull: SKIN injuries
        Type: general
      – SubjectFull: WOUND healing
        Type: general
      – SubjectFull: BIOLOGICAL models
        Type: general
      – SubjectFull: PHOSPHORYLATION
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      – SubjectFull: CELL proliferation
        Type: general
      – SubjectFull: CELLULAR signal transduction
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      – SubjectFull: CYTOSKELETAL proteins
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      – SubjectFull: TREATMENT effectiveness
        Type: general
      – SubjectFull: FLUORESCENT antibody technique
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      – SubjectFull: MICE
        Type: general
      – SubjectFull: IMMUNOHISTOCHEMISTRY
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      – SubjectFull: GLYCOSIDES
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      – SubjectFull: ANIMAL experimentation
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      – SubjectFull: WESTERN immunoblotting
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      – SubjectFull: BENZOPYRANS
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      – SubjectFull: COMPARATIVE studies
        Type: general
    Titles:
      – TitleFull: Esculin promotes skin wound healing in mice and regulates the Wnt/β-catenin signaling pathway.
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            – D: 12
              M: 03
              Text: 3/12/2025
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              Y: 2025
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