Improving the alcohol respiratory chain and energy metabolism by enhancing PQQ synthesis in Acetobacter pasteurianus.
| Title: | Improving the alcohol respiratory chain and energy metabolism by enhancing PQQ synthesis in Acetobacter pasteurianus. |
|---|---|
| Authors: | Zhang, Wenqing1 (AUTHOR), Feng, Chen1 (AUTHOR), Zhang, Chunxue1 (AUTHOR), Song, Jia1 (AUTHOR), Li, Li2 (AUTHOR), Xia, Menglei1 (AUTHOR), Ding, Wei3 (AUTHOR), Zheng, Yu1,3,4 (AUTHOR), Wang, Min1 (AUTHOR) |
| Source: | Journal of Industrial Microbiology & Biotechnology. 2024, Vol. 51, p1-9. 9p. |
| Subject Terms: | *ACETOBACTER, *PQQ (Biochemistry), *ALDEHYDE dehydrogenase, *ALCOHOL dehydrogenase, *ENERGY metabolism |
| Abstract: | Pyrroloquinoline quinone (PQQ) is one of the important coenzymes in living organisms. In acetic acid bacteria (AAB), it plays a crucial role in the alcohol respiratory chain, as a coenzyme of alcohol dehydrogenase (ADH). In this work, the PQQ biosynthetic genes were overexpressed in Acetobacter pasteurianus CGMCC 3089 to improve the fermentation performance. The result shows that the intracellular and extracellular PQQ contents in the recombinant strain A. pasteurianus (pBBR1-p264- pqq) were 152.53% and 141.08% higher than those of the control A. pasteurianus (pBBR1-p264), respectively. The catalytic activity of ADH and aldehyde dehydrogenase increased by 52.92% and 67.04%, respectively. The results indicated that the energy charge and intracellular ATP were also improved in the recombinant strain. The acetic acid fermentation was carried out using a 5 L self-aspirating fermenter, and the acetic acid production rate of the recombinant strain was 23.20% higher compared with the control. Furthermore, the relationship between the PQQ and acetic acid tolerance of cells was analyzed. The biomass of recombinant strain was 180.2%, 44.3%, and 38.6% higher than those of control under 2%, 3%, and 4% acetic acid stress, respectively. After being treated with 6% acetic acid for 40 min, the survival rate of the recombinant strain was increased by 76.20% compared with the control. Those results demonstrated that overexpression of PQQ biosynthetic genes increased the content of PQQ, therefore improving the acetic acid fermentation and the cell tolerance against acetic acid by improving the alcohol respiratory chain and energy metabolism. One Sentence Summary The increase in PQQ content enhances the activity of the alcohol respiratory chain of Acetobacter pasteurianus , and the increase in energy charge enhances the tolerance of cells against acetic acid, therefore, improving the efficiency of acetic acid fermentation. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Improving the alcohol respiratory chain and energy metabolism by enhancing PQQ synthesis in Acetobacter pasteurianus. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Wenqing%22">Zhang, Wenqing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Feng%2C+Chen%22">Feng, Chen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Chunxue%22">Zhang, Chunxue</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Song%2C+Jia%22">Song, Jia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Li%22">Li, Li</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xia%2C+Menglei%22">Xia, Menglei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ding%2C+Wei%22">Ding, Wei</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Yu%22">Zheng, Yu</searchLink><relatesTo>1,3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Min%22">Wang, Min</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+Microbiology+%26+Biotechnology%22">Journal of Industrial Microbiology & Biotechnology</searchLink>. 2024, Vol. 51, p1-9. 9p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22ACETOBACTER%22">ACETOBACTER</searchLink><br />*<searchLink fieldCode="DE" term="%22PQQ+%28Biochemistry%29%22">PQQ (Biochemistry)</searchLink><br />*<searchLink fieldCode="DE" term="%22ALDEHYDE+dehydrogenase%22">ALDEHYDE dehydrogenase</searchLink><br />*<searchLink fieldCode="DE" term="%22ALCOHOL+dehydrogenase%22">ALCOHOL dehydrogenase</searchLink><br />*<searchLink fieldCode="DE" term="%22ENERGY+metabolism%22">ENERGY metabolism</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Pyrroloquinoline quinone (PQQ) is one of the important coenzymes in living organisms. In acetic acid bacteria (AAB), it plays a crucial role in the alcohol respiratory chain, as a coenzyme of alcohol dehydrogenase (ADH). In this work, the PQQ biosynthetic genes were overexpressed in Acetobacter pasteurianus CGMCC 3089 to improve the fermentation performance. The result shows that the intracellular and extracellular PQQ contents in the recombinant strain A. pasteurianus (pBBR1-p264- pqq) were 152.53% and 141.08% higher than those of the control A. pasteurianus (pBBR1-p264), respectively. The catalytic activity of ADH and aldehyde dehydrogenase increased by 52.92% and 67.04%, respectively. The results indicated that the energy charge and intracellular ATP were also improved in the recombinant strain. The acetic acid fermentation was carried out using a 5 L self-aspirating fermenter, and the acetic acid production rate of the recombinant strain was 23.20% higher compared with the control. Furthermore, the relationship between the PQQ and acetic acid tolerance of cells was analyzed. The biomass of recombinant strain was 180.2%, 44.3%, and 38.6% higher than those of control under 2%, 3%, and 4% acetic acid stress, respectively. After being treated with 6% acetic acid for 40 min, the survival rate of the recombinant strain was increased by 76.20% compared with the control. Those results demonstrated that overexpression of PQQ biosynthetic genes increased the content of PQQ, therefore improving the acetic acid fermentation and the cell tolerance against acetic acid by improving the alcohol respiratory chain and energy metabolism. One Sentence Summary The increase in PQQ content enhances the activity of the alcohol respiratory chain of Acetobacter pasteurianus , and the increase in energy charge enhances the tolerance of cells against acetic acid, therefore, improving the efficiency of acetic acid fermentation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1093/jimb/kuae036 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: ACETOBACTER Type: general – SubjectFull: PQQ (Biochemistry) Type: general – SubjectFull: ALDEHYDE dehydrogenase Type: general – SubjectFull: ALCOHOL dehydrogenase Type: general – SubjectFull: ENERGY metabolism Type: general Titles: – TitleFull: Improving the alcohol respiratory chain and energy metabolism by enhancing PQQ synthesis in Acetobacter pasteurianus. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zhang, Wenqing – PersonEntity: Name: NameFull: Feng, Chen – PersonEntity: Name: NameFull: Zhang, Chunxue – PersonEntity: Name: NameFull: Song, Jia – PersonEntity: Name: NameFull: Li, Li – PersonEntity: Name: NameFull: Xia, Menglei – PersonEntity: Name: NameFull: Ding, Wei – PersonEntity: Name: NameFull: Zheng, Yu – PersonEntity: Name: NameFull: Wang, Min IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 13675435 Numbering: – Type: volume Value: 51 Titles: – TitleFull: Journal of Industrial Microbiology & Biotechnology Type: main |
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