Academic Journal
View in EDS

Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.

Bibliographic Details
Title: Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.
Authors: Girardi, Laura1 (AUTHOR) laura.girardi@igenomix.com, Patassini, Cristina1 (AUTHOR), Miravet Valenciano, Jose2 (AUTHOR), Sato, Yoshimi3 (AUTHOR), Fagundes Cagnin, Natalia4 (AUTHOR), Castellón, Jose Antonio5 (AUTHOR), Cogo, Francesco1 (AUTHOR), Zambon, Paola1 (AUTHOR), Blesa, David2 (AUTHOR), Jimenez Almazan, Jorge2 (AUTHOR), Akinwole, Adedoyin6 (AUTHOR), Coprerski, Bruno4 (AUTHOR), Rubio, Carmen2 (AUTHOR)
Source: Journal of Assisted Reproduction & Genetics. Dec2024, Vol. 41 Issue 12, p3357-3370. 14p.
Subject Terms: *DIPLOIDY, *HAPLOIDY, *PLOIDY, *MATERNAL age, *GENETIC testing, *BLASTOCYST
Abstract: Purpose: We aimed to identify the correlation between morphological pronuclear (PN) status and the genetically determined ploidy configuration in preimplantation embryos. Methods: A retrospective observational study was conducted on 1982 embryos displaying normal fertilization and 380 embryos showing an atypical PN pattern, tested for aneuploidies and ploidy status via preimplantation genetic testing (PGT) between May 2019 and May 2024. Ploidy prediction was performed using a validated targeted-NGS approach and a proprietary bioinformatic pipeline analyzing SNPs B-allele frequency information. Ploidy results were obtained in relation to the morphological PN pattern and further stratified by mode of PN observation, maternal age, and embryo quality parameters. Results: Abnormal ploidy results in 2PN-derived embryos were 1% (n = 20/1982): 0.8% showed triploidy and 0.2% haploidy. Ploidy results in relation to PN number in atypical fertilization were as follows: 0PN (n = 150/380) associated with 87.3% of diploidy, 8.7% of haploidy, and 4.0% of triploidy; 1PN-derived blastocysts (n = 73/153) were haploid in 47.7% of cases, 6.5% were triploid, and 45.7% diploid; 2.1PN (n = 23/280) and 3PN patterns (n = 54/280) predicted a triploid result in 34.8% and 74.1% of cases, respectively. PN observation with time-lapse increased ploidy status predictivity from 28.3% to 80.4% (p < 0.01) and reduced expected diploid rates to 19.6% (p < 0.01). Diploidy rate was higher for maternal age ≤ 35 years and for morphologically high-grade embryos. Conclusion: Morphological PN check can be improved by incorporating ploidy analysis within the conventional PGT workflow. Euploid 2PN-derived embryos can be further selected removing haploids and triploids, and some atypical PN pattern can be better classified. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Assisted Reproduction & Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Academic Search Complete
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:a9h&genre=article&issn=10580468&ISBN=&volume=41&issue=12&date=20241201&spage=3357&pages=3357-3370&title=Journal of Assisted Reproduction & Genetics&atitle=Incidence%20of%20haploidy%20and%20triploidy%20in%20trophectoderm%20biopsies%20of%20blastocysts%20derived%20from%20normally%20and%20abnormally%20fertilized%20oocytes.&aulast=Girardi%2C%20Laura&id=DOI:10.1007/s10815-024-03278-4
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
Header DbId: a9h
DbLabel: Academic Search Complete
An: 182100044
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Girardi%2C+Laura%22&quot;&gt;Girardi, Laura&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; laura.girardi@igenomix.com&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Patassini%2C+Cristina%22&quot;&gt;Patassini, Cristina&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Miravet+Valenciano%2C+Jose%22&quot;&gt;Miravet Valenciano, Jose&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Sato%2C+Yoshimi%22&quot;&gt;Sato, Yoshimi&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Fagundes+Cagnin%2C+Natalia%22&quot;&gt;Fagundes Cagnin, Natalia&lt;/searchLink&gt;&lt;relatesTo&gt;4&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Castell&#243;n%2C+Jose+Antonio%22&quot;&gt;Castell&#243;n, Jose Antonio&lt;/searchLink&gt;&lt;relatesTo&gt;5&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cogo%2C+Francesco%22&quot;&gt;Cogo, Francesco&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zambon%2C+Paola%22&quot;&gt;Zambon, Paola&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Blesa%2C+David%22&quot;&gt;Blesa, David&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Jimenez+Almazan%2C+Jorge%22&quot;&gt;Jimenez Almazan, Jorge&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Akinwole%2C+Adedoyin%22&quot;&gt;Akinwole, Adedoyin&lt;/searchLink&gt;&lt;relatesTo&gt;6&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Coprerski%2C+Bruno%22&quot;&gt;Coprerski, Bruno&lt;/searchLink&gt;&lt;relatesTo&gt;4&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Rubio%2C+Carmen%22&quot;&gt;Rubio, Carmen&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Journal+of+Assisted+Reproduction+%26+Genetics%22&quot;&gt;Journal of Assisted Reproduction &amp; Genetics&lt;/searchLink&gt;. Dec2024, Vol. 41 Issue 12, p3357-3370. 14p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22DIPLOIDY%22&quot;&gt;DIPLOIDY&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22HAPLOIDY%22&quot;&gt;HAPLOIDY&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22PLOIDY%22&quot;&gt;PLOIDY&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22MATERNAL+age%22&quot;&gt;MATERNAL age&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22GENETIC+testing%22&quot;&gt;GENETIC testing&lt;/searchLink&gt;&lt;br /&gt;*&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22BLASTOCYST%22&quot;&gt;BLASTOCYST&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Purpose: We aimed to identify the correlation between morphological pronuclear (PN) status and the genetically determined ploidy configuration in preimplantation embryos. Methods: A retrospective observational study was conducted on 1982 embryos displaying normal fertilization and 380 embryos showing an atypical PN pattern, tested for aneuploidies and ploidy status via preimplantation genetic testing (PGT) between May 2019 and May 2024. Ploidy prediction was performed using a validated targeted-NGS approach and a proprietary bioinformatic pipeline analyzing SNPs B-allele frequency information. Ploidy results were obtained in relation to the morphological PN pattern and further stratified by mode of PN observation, maternal age, and embryo quality parameters. Results: Abnormal ploidy results in 2PN-derived embryos were 1% (n = 20/1982): 0.8% showed triploidy and 0.2% haploidy. Ploidy results in relation to PN number in atypical fertilization were as follows: 0PN (n = 150/380) associated with 87.3% of diploidy, 8.7% of haploidy, and 4.0% of triploidy; 1PN-derived blastocysts (n = 73/153) were haploid in 47.7% of cases, 6.5% were triploid, and 45.7% diploid; 2.1PN (n = 23/280) and 3PN patterns (n = 54/280) predicted a triploid result in 34.8% and 74.1% of cases, respectively. PN observation with time-lapse increased ploidy status predictivity from 28.3% to 80.4% (p &lt; 0.01) and reduced expected diploid rates to 19.6% (p &lt; 0.01). Diploidy rate was higher for maternal age ≤ 35 years and for morphologically high-grade embryos. Conclusion: Morphological PN check can be improved by incorporating ploidy analysis within the conventional PGT workflow. Euploid 2PN-derived embryos can be further selected removing haploids and triploids, and some atypical PN pattern can be better classified. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of Journal of Assisted Reproduction &amp; Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder&#39;s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=a9h&AN=182100044
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10815-024-03278-4
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 3357
    Subjects:
      – SubjectFull: DIPLOIDY
        Type: general
      – SubjectFull: HAPLOIDY
        Type: general
      – SubjectFull: PLOIDY
        Type: general
      – SubjectFull: MATERNAL age
        Type: general
      – SubjectFull: GENETIC testing
        Type: general
      – SubjectFull: BLASTOCYST
        Type: general
    Titles:
      – TitleFull: Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Girardi, Laura
      – PersonEntity:
          Name:
            NameFull: Patassini, Cristina
      – PersonEntity:
          Name:
            NameFull: Miravet Valenciano, Jose
      – PersonEntity:
          Name:
            NameFull: Sato, Yoshimi
      – PersonEntity:
          Name:
            NameFull: Fagundes Cagnin, Natalia
      – PersonEntity:
          Name:
            NameFull: Castellón, Jose Antonio
      – PersonEntity:
          Name:
            NameFull: Cogo, Francesco
      – PersonEntity:
          Name:
            NameFull: Zambon, Paola
      – PersonEntity:
          Name:
            NameFull: Blesa, David
      – PersonEntity:
          Name:
            NameFull: Jimenez Almazan, Jorge
      – PersonEntity:
          Name:
            NameFull: Akinwole, Adedoyin
      – PersonEntity:
          Name:
            NameFull: Coprerski, Bruno
      – PersonEntity:
          Name:
            NameFull: Rubio, Carmen
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 12
              Text: Dec2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 10580468
          Numbering:
            – Type: volume
              Value: 41
            – Type: issue
              Value: 12
          Titles:
            – TitleFull: Journal of Assisted Reproduction & Genetics
              Type: main
ResultId 1