Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers.
| Title: | Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers. |
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| Authors: | Leach, Benjamin I.1 (AUTHOR), Lister, Deanne2 (AUTHOR), Adams, Stephen R.3 (AUTHOR), Bykowski, Julie1 (AUTHOR), Schwartz, Amy B.1 (AUTHOR), McConville, Patrick2 (AUTHOR), Dimant, Hemi2 (AUTHOR), Ahrens, Eric T.1 (AUTHOR) etanospam@gmail.com |
| Source: | Molecular Imaging & Biology. Oct2024, Vol. 26 Issue 5, p888-898. 11p. |
| Subject Terms: | *MAGNETIC resonance imaging, *RETICULO-endothelial system, *HIGH resolution imaging, *FLUORESCENCE quenching, *CELL populations |
| Abstract: | Purpose: We explore the use of intravenously delivered fluorescent perfluorocarbon (PFC) nanoemulsion tracers and multi-spectral cryo-fluorescence tomography (CFT) for whole-body tracer imaging in murine inflammation models. CFT is an emerging technique that provides high-resolution, three-dimensional mapping of probe localization in intact animals and tissue samples, enabling unbiased validation of probe biodistribution and minimizes reliance on laborious histological methods employing discrete tissue panels, where disseminated populations of PFC-labeled cells may be overlooked. This methodology can be used to streamline the development of new generations of non-invasive, cellular-molecular imaging probes for in vivo imaging. Procedures: Mixtures of nanoemulsions with different fluorescent emission wavelengths were administered intravenously to naïve mice and models of acute inflammation, colitis, and solid tumor. Mice were euthanized 24 h post-injection, frozen en bloc, and imaged at high resolution (~ 50 µm voxels) using CFT at multiple wavelengths. Results: PFC nanoemulsions were visualized using CFT within tissues of the reticuloendothelial system and inflammatory lesions, consistent with immune cell (macrophage) labeling, as previously reported in in vivo magnetic resonance and nuclear imaging studies. The CFT signals show pronounced differences among fluorescence wavelengths and tissues, presumably due to autofluorescence, differential fluorescence quenching, and scattering of incident and emitted light. Conclusions: CFT is an effective and complementary methodology to in vivo imaging for validating PFC nanoemulsion biodistribution at high spatial localization, bridging the resolution gap between in vivo imaging and histology. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Leach%2C+Benjamin+I%2E%22">Leach, Benjamin I.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lister%2C+Deanne%22">Lister, Deanne</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Adams%2C+Stephen+R%2E%22">Adams, Stephen R.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bykowski%2C+Julie%22">Bykowski, Julie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schwartz%2C+Amy+B%2E%22">Schwartz, Amy B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22McConville%2C+Patrick%22">McConville, Patrick</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dimant%2C+Hemi%22">Dimant, Hemi</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ahrens%2C+Eric+T%2E%22">Ahrens, Eric T.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> etanospam@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Molecular+Imaging+%26+Biology%22">Molecular Imaging & Biology</searchLink>. Oct2024, Vol. 26 Issue 5, p888-898. 11p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22MAGNETIC+resonance+imaging%22">MAGNETIC resonance imaging</searchLink><br />*<searchLink fieldCode="DE" term="%22RETICULO-endothelial+system%22">RETICULO-endothelial system</searchLink><br />*<searchLink fieldCode="DE" term="%22HIGH+resolution+imaging%22">HIGH resolution imaging</searchLink><br />*<searchLink fieldCode="DE" term="%22FLUORESCENCE+quenching%22">FLUORESCENCE quenching</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+populations%22">CELL populations</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Purpose: We explore the use of intravenously delivered fluorescent perfluorocarbon (PFC) nanoemulsion tracers and multi-spectral cryo-fluorescence tomography (CFT) for whole-body tracer imaging in murine inflammation models. CFT is an emerging technique that provides high-resolution, three-dimensional mapping of probe localization in intact animals and tissue samples, enabling unbiased validation of probe biodistribution and minimizes reliance on laborious histological methods employing discrete tissue panels, where disseminated populations of PFC-labeled cells may be overlooked. This methodology can be used to streamline the development of new generations of non-invasive, cellular-molecular imaging probes for in vivo imaging. Procedures: Mixtures of nanoemulsions with different fluorescent emission wavelengths were administered intravenously to naïve mice and models of acute inflammation, colitis, and solid tumor. Mice were euthanized 24 h post-injection, frozen en bloc, and imaged at high resolution (~ 50 µm voxels) using CFT at multiple wavelengths. Results: PFC nanoemulsions were visualized using CFT within tissues of the reticuloendothelial system and inflammatory lesions, consistent with immune cell (macrophage) labeling, as previously reported in in vivo magnetic resonance and nuclear imaging studies. The CFT signals show pronounced differences among fluorescence wavelengths and tissues, presumably due to autofluorescence, differential fluorescence quenching, and scattering of incident and emitted light. Conclusions: CFT is an effective and complementary methodology to in vivo imaging for validating PFC nanoemulsion biodistribution at high spatial localization, bridging the resolution gap between in vivo imaging and histology. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Molecular Imaging & Biology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s11307-024-01926-w Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 888 Subjects: – SubjectFull: MAGNETIC resonance imaging Type: general – SubjectFull: RETICULO-endothelial system Type: general – SubjectFull: HIGH resolution imaging Type: general – SubjectFull: FLUORESCENCE quenching Type: general – SubjectFull: CELL populations Type: general Titles: – TitleFull: Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Leach, Benjamin I. – PersonEntity: Name: NameFull: Lister, Deanne – PersonEntity: Name: NameFull: Adams, Stephen R. – PersonEntity: Name: NameFull: Bykowski, Julie – PersonEntity: Name: NameFull: Schwartz, Amy B. – PersonEntity: Name: NameFull: McConville, Patrick – PersonEntity: Name: NameFull: Dimant, Hemi – PersonEntity: Name: NameFull: Ahrens, Eric T. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 15361632 Numbering: – Type: volume Value: 26 – Type: issue Value: 5 Titles: – TitleFull: Molecular Imaging & Biology Type: main |
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