MiRNA expression profiling reveals a potential role of microRNA-148b-3p in cerebral vasospasm in subarachnoid hemorrhage.
| Title: | MiRNA expression profiling reveals a potential role of microRNA-148b-3p in cerebral vasospasm in subarachnoid hemorrhage. |
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| Authors: | Ryu, Jee-Yeon1 (AUTHOR), Zhang, Jianing1 (AUTHOR), Tirado, Selena-Rae1 (AUTHOR), Dagen, Sarajune1 (AUTHOR), Frerichs, Kai U.1 (AUTHOR), Patel, Nirav J.1 (AUTHOR), Aziz-Sultan, M. Ali1 (AUTHOR), Brown, Alison2 (AUTHOR), Rogers-Grazado, Maximilian2 (AUTHOR), Amr, Sami S.2 (AUTHOR), Weiss, Scott T.3 (AUTHOR), Du, Rose1 (AUTHOR) rdu@bwh.harvard.edu |
| Source: | Scientific Reports. 9/29/2024, Vol. 14 Issue 1, p1-12. 12p. |
| Subject Terms: | *GENE expression, *CEREBRAL vasospasm, *SUBARACHNOID hemorrhage, *CEREBRAL hemorrhage, *CEREBRAL ischemia |
| Abstract: | Cerebral vasospasm (CVS) is an important contributor to delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage (aSAH), leading to high morbidity and long-term disability. While several microRNAs (miRNAs) have been implicated in vasospasm, the underlying mechanisms for CVS remain poorly understood. Our study aims to identify miRNAs that may contribute to the development of CVS. Whole-blood samples were obtained during or outside of vasospasm from aSAH patients whose maximal vasospasm was moderate or severe. MiRNAs were isolated from serial whole-blood samples, and miRNA sequencing was performed. Differentially expressed miRNAs were identified and the expression levels in patients' samples were verified using real-time qPCR. The biological functions of identified miRNA were evaluated in human brain endothelial cells (HBECs). MiRNA profiling revealed significant upregulation of miR-148b-3p in patients during CVS. We demonstrated that miR-148b-3p directly targeted and decreased the expression of ROCK1, affecting cell proliferation, migration, and invasion of HBECs through the ROCK-LIMK-Cofilin pathway. We propose that the upregulation of miRNA-148b-3p plays a role in the development of CVS by regulating actin cytoskeletal dynamics in HBECs, which is crucial for vascular function. Our study highlights miR-148b-3p as a potential diagnostic marker as well as therapeutic target for CVS following aSAH. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: MiRNA expression profiling reveals a potential role of microRNA-148b-3p in cerebral vasospasm in subarachnoid hemorrhage. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ryu%2C+Jee-Yeon%22">Ryu, Jee-Yeon</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jianing%22">Zhang, Jianing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tirado%2C+Selena-Rae%22">Tirado, Selena-Rae</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dagen%2C+Sarajune%22">Dagen, Sarajune</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Frerichs%2C+Kai+U%2E%22">Frerichs, Kai U.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Patel%2C+Nirav+J%2E%22">Patel, Nirav J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Aziz-Sultan%2C+M%2E+Ali%22">Aziz-Sultan, M. Ali</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Brown%2C+Alison%22">Brown, Alison</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rogers-Grazado%2C+Maximilian%22">Rogers-Grazado, Maximilian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Amr%2C+Sami+S%2E%22">Amr, Sami S.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Weiss%2C+Scott+T%2E%22">Weiss, Scott T.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Du%2C+Rose%22">Du, Rose</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> rdu@bwh.harvard.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Scientific+Reports%22">Scientific Reports</searchLink>. 9/29/2024, Vol. 14 Issue 1, p1-12. 12p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br />*<searchLink fieldCode="DE" term="%22CEREBRAL+vasospasm%22">CEREBRAL vasospasm</searchLink><br />*<searchLink fieldCode="DE" term="%22SUBARACHNOID+hemorrhage%22">SUBARACHNOID hemorrhage</searchLink><br />*<searchLink fieldCode="DE" term="%22CEREBRAL+hemorrhage%22">CEREBRAL hemorrhage</searchLink><br />*<searchLink fieldCode="DE" term="%22CEREBRAL+ischemia%22">CEREBRAL ischemia</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Cerebral vasospasm (CVS) is an important contributor to delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage (aSAH), leading to high morbidity and long-term disability. While several microRNAs (miRNAs) have been implicated in vasospasm, the underlying mechanisms for CVS remain poorly understood. Our study aims to identify miRNAs that may contribute to the development of CVS. Whole-blood samples were obtained during or outside of vasospasm from aSAH patients whose maximal vasospasm was moderate or severe. MiRNAs were isolated from serial whole-blood samples, and miRNA sequencing was performed. Differentially expressed miRNAs were identified and the expression levels in patients' samples were verified using real-time qPCR. The biological functions of identified miRNA were evaluated in human brain endothelial cells (HBECs). MiRNA profiling revealed significant upregulation of miR-148b-3p in patients during CVS. We demonstrated that miR-148b-3p directly targeted and decreased the expression of ROCK1, affecting cell proliferation, migration, and invasion of HBECs through the ROCK-LIMK-Cofilin pathway. We propose that the upregulation of miRNA-148b-3p plays a role in the development of CVS by regulating actin cytoskeletal dynamics in HBECs, which is crucial for vascular function. Our study highlights miR-148b-3p as a potential diagnostic marker as well as therapeutic target for CVS following aSAH. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/s41598-024-73579-2 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: GENE expression Type: general – SubjectFull: CEREBRAL vasospasm Type: general – SubjectFull: SUBARACHNOID hemorrhage Type: general – SubjectFull: CEREBRAL hemorrhage Type: general – SubjectFull: CEREBRAL ischemia Type: general Titles: – TitleFull: MiRNA expression profiling reveals a potential role of microRNA-148b-3p in cerebral vasospasm in subarachnoid hemorrhage. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ryu, Jee-Yeon – PersonEntity: Name: NameFull: Zhang, Jianing – PersonEntity: Name: NameFull: Tirado, Selena-Rae – PersonEntity: Name: NameFull: Dagen, Sarajune – PersonEntity: Name: NameFull: Frerichs, Kai U. – PersonEntity: Name: NameFull: Patel, Nirav J. – PersonEntity: Name: NameFull: Aziz-Sultan, M. Ali – PersonEntity: Name: NameFull: Brown, Alison – PersonEntity: Name: NameFull: Rogers-Grazado, Maximilian – PersonEntity: Name: NameFull: Amr, Sami S. – PersonEntity: Name: NameFull: Weiss, Scott T. – PersonEntity: Name: NameFull: Du, Rose IsPartOfRelationships: – BibEntity: Dates: – D: 29 M: 09 Text: 9/29/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20452322 Numbering: – Type: volume Value: 14 – Type: issue Value: 1 Titles: – TitleFull: Scientific Reports Type: main |
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