Antioxidative and Antiglycative Stress Activities of Selenoglutathione Diselenide.
| Title: | Antioxidative and Antiglycative Stress Activities of Selenoglutathione Diselenide. |
|---|---|
| Authors: | Kanamori, Akiko1,2 (AUTHOR) akanamori@tokai.ac.jp, Egawa, Nana1 (AUTHOR) denquasuyako@icloud.com, Yamasaki, Suyako1 (AUTHOR), Ikeda, Takehito3 (AUTHOR) basketball.boy41@icloud.com, da Rocha, Marcia Juciele4 (AUTHOR) marciajr_15@hotmail.com, Bortolatto, Cristiani Folharini4 (AUTHOR) cbortolatto@gmail.com, Savegnago, Lucielli5 (AUTHOR) luciellisavegnago@yahoo.com.br, Brüning, César Augusto4 (AUTHOR) cabruning@yahoo.com.br, Iwaoka, Michio2,3 (AUTHOR) akanamori@tokai.ac.jp |
| Source: | Pharmaceuticals (14248247). Aug2024, Vol. 17 Issue 8, p1049. 14p. |
| Subject Terms: | *ADVANCED glycation end-products, *GLUTATHIONE reductase, *HAZARDOUS substances, *GLUTATHIONE peroxidase, *OXIDANT status |
| Abstract: | The damage caused by oxidative and glycative stress to cells accumulates on a daily basis and accelerates aging. Glutathione (GSH), a major antioxidant molecule in living organisms, plays a crucial role in detoxifying the stress-causing substances inherent in cells, such as H2O2 and methylglyoxal (MG), an important intermediate of advanced glycation end-products (AGEs). In this study, we focused on the enhanced antioxidant capacity of the selenium analog of GSH, i.e., selenoglutathione (GSeH), compared to GSH, and examined its effects on the detoxification of stress-causing substances and improvement in cell viability. In cell-free systems, GSeH (1 mM) generated in situ from GSeSeG in the presence of NADPH and glutathione reductase (GR) rapidly reduced more than 80% of 0.1 mM H2O2, indicating the significant glutathione peroxidase (GPx)-like antioxidant activity of GSeSeG. Similarly, around 50% of 0.5 mM MG was degraded by 0.5 mM GSeH within 30 min through a non-enzymatic mechanism. It was also found that GSeSeG (0.05–0.5 mM) showed glutathione S-transferase (GST)-like activity against 1-chloro-2,4-dinitrobenzene (CDNB), a model substance of oxidative stress-causing toxic materials in cells. Meanwhile, HeLa cells that had been pre-treated with GSeSeG exhibited increased viability against 1.2 mM H2O2 (at [GSeSeG] = 0.5–50 μM) and 4 mM MG (at [GSeSeG] = 3 μM), and the latter effect was maintained for two days. Thus, GSeSeG is a potential antioxidant and antiglycative stress agent for cells. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Antioxidative and Antiglycative Stress Activities of Selenoglutathione Diselenide. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kanamori%2C+Akiko%22">Kanamori, Akiko</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> akanamori@tokai.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Egawa%2C+Nana%22">Egawa, Nana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> denquasuyako@icloud.com</i><br /><searchLink fieldCode="AR" term="%22Yamasaki%2C+Suyako%22">Yamasaki, Suyako</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ikeda%2C+Takehito%22">Ikeda, Takehito</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> basketball.boy41@icloud.com</i><br /><searchLink fieldCode="AR" term="%22da+Rocha%2C+Marcia+Juciele%22">da Rocha, Marcia Juciele</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> marciajr_15@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Bortolatto%2C+Cristiani+Folharini%22">Bortolatto, Cristiani Folharini</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> cbortolatto@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Savegnago%2C+Lucielli%22">Savegnago, Lucielli</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> luciellisavegnago@yahoo.com.br</i><br /><searchLink fieldCode="AR" term="%22Brüning%2C+César+Augusto%22">Brüning, César Augusto</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> cabruning@yahoo.com.br</i><br /><searchLink fieldCode="AR" term="%22Iwaoka%2C+Michio%22">Iwaoka, Michio</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> akanamori@tokai.ac.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Pharmaceuticals+%2814248247%29%22">Pharmaceuticals (14248247)</searchLink>. Aug2024, Vol. 17 Issue 8, p1049. 14p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22ADVANCED+glycation+end-products%22">ADVANCED glycation end-products</searchLink><br />*<searchLink fieldCode="DE" term="%22GLUTATHIONE+reductase%22">GLUTATHIONE reductase</searchLink><br />*<searchLink fieldCode="DE" term="%22HAZARDOUS+substances%22">HAZARDOUS substances</searchLink><br />*<searchLink fieldCode="DE" term="%22GLUTATHIONE+peroxidase%22">GLUTATHIONE peroxidase</searchLink><br />*<searchLink fieldCode="DE" term="%22OXIDANT+status%22">OXIDANT status</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The damage caused by oxidative and glycative stress to cells accumulates on a daily basis and accelerates aging. Glutathione (GSH), a major antioxidant molecule in living organisms, plays a crucial role in detoxifying the stress-causing substances inherent in cells, such as H2O2 and methylglyoxal (MG), an important intermediate of advanced glycation end-products (AGEs). In this study, we focused on the enhanced antioxidant capacity of the selenium analog of GSH, i.e., selenoglutathione (GSeH), compared to GSH, and examined its effects on the detoxification of stress-causing substances and improvement in cell viability. In cell-free systems, GSeH (1 mM) generated in situ from GSeSeG in the presence of NADPH and glutathione reductase (GR) rapidly reduced more than 80% of 0.1 mM H2O2, indicating the significant glutathione peroxidase (GPx)-like antioxidant activity of GSeSeG. Similarly, around 50% of 0.5 mM MG was degraded by 0.5 mM GSeH within 30 min through a non-enzymatic mechanism. It was also found that GSeSeG (0.05–0.5 mM) showed glutathione S-transferase (GST)-like activity against 1-chloro-2,4-dinitrobenzene (CDNB), a model substance of oxidative stress-causing toxic materials in cells. Meanwhile, HeLa cells that had been pre-treated with GSeSeG exhibited increased viability against 1.2 mM H2O2 (at [GSeSeG] = 0.5–50 μM) and 4 mM MG (at [GSeSeG] = 3 μM), and the latter effect was maintained for two days. Thus, GSeSeG is a potential antioxidant and antiglycative stress agent for cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Pharmaceuticals (14248247) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/ph17081049 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 1049 Subjects: – SubjectFull: ADVANCED glycation end-products Type: general – SubjectFull: GLUTATHIONE reductase Type: general – SubjectFull: HAZARDOUS substances Type: general – SubjectFull: GLUTATHIONE peroxidase Type: general – SubjectFull: OXIDANT status Type: general Titles: – TitleFull: Antioxidative and Antiglycative Stress Activities of Selenoglutathione Diselenide. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kanamori, Akiko – PersonEntity: Name: NameFull: Egawa, Nana – PersonEntity: Name: NameFull: Yamasaki, Suyako – PersonEntity: Name: NameFull: Ikeda, Takehito – PersonEntity: Name: NameFull: da Rocha, Marcia Juciele – PersonEntity: Name: NameFull: Bortolatto, Cristiani Folharini – PersonEntity: Name: NameFull: Savegnago, Lucielli – PersonEntity: Name: NameFull: Brüning, César Augusto – PersonEntity: Name: NameFull: Iwaoka, Michio IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 14248247 Numbering: – Type: volume Value: 17 – Type: issue Value: 8 Titles: – TitleFull: Pharmaceuticals (14248247) Type: main |
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