Academic Journal
View in EDS
HTML Full Text

Identification of two plastid transit peptides for construction of pollen-inactivation system in rice.

Bibliographic Details
Title: Identification of two plastid transit peptides for construction of pollen-inactivation system in rice.
Authors: Wang, Menglong1,2 (AUTHOR), Peng, Xiaoqun1,2 (AUTHOR), Wang, Changjian2 (AUTHOR), Tang, Xiaoyan2,3,4 (AUTHOR) txy@frontier-ag.com
Source: Molecular Breeding. May2024, Vol. 44 Issue 5, p1-14. 14p.
Subject Terms: *MALE sterility in plants, *HYBRID rice, *SEED technology, *SIGNAL peptides, *RICE, *PEPTIDES, *CARRIER proteins
Abstract: Hybrid seed production technology (SPT) is achieved through the utilization of a recessive nuclear male-sterile mutant transformed with a transgenic cassette comprising three essential components: the wild-type gene to restore the fertility of the male-sterile mutant, an α-amylase gene to disrupt transgenic pollen grains, and red fluorescence protein gene DsRed to distinguish the transgenic seeds from the nontransgenic male sterile seeds. In rice, we establish the pollen disruption system by introducing an amyloplast targeting signal peptide (ASP) at the N-terminus of maize α-amylase protein ZM-AA1ΔSP (ZM-AA1 with the N-terminal signal peptide removed). The ASP facilitates the transport of ZM-AA1ΔSP protein into amyloplast where it degrades starch, resulting in disruption of the pollen fertility. To obtain such signal peptides for rice, we searched the rice proteins homologous to the defined wheat amyloplast proteins followed by protein–protein interaction network predictions and targeting signal peptides prediction. These analyses enabled the identification of four candidate ASPs in rice, which were designated as ASP1, ASP2, ASP3, and ASP4, respectively. ASP1 and ASP2, when linked with ZM-AA1ΔSP, exhibited the capability to disrupt transgenic pollen grains, whereas ASP3 and ASP4 did not produce this effect. Interestingly, the localization experiments showed that ASP3 and ASP4 were able to target the proteins into chloroplast. The ASP1 and ASP2 sequences provide valuable tools for genetic engineering of the rice male-sterile system, which will contribute to the hybrid rice breeding and production. [ABSTRACT FROM AUTHOR]
Copyright of Molecular Breeding is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Academic Search Complete
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:a9h&genre=article&issn=13803743&ISBN=&volume=44&issue=5&date=20240501&spage=1&pages=1-14&title=Molecular Breeding&atitle=Identification%20of%20two%20plastid%20transit%20peptides%20for%20construction%20of%20pollen-inactivation%20system%20in%20rice.&aulast=Wang%2C%20Menglong&id=DOI:10.1007/s11032-024-01471-y
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
Header DbId: a9h
DbLabel: Academic Search Complete
An: 177816616
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Identification of two plastid transit peptides for construction of pollen-inactivation system in rice.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Menglong%22">Wang, Menglong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Peng%2C+Xiaoqun%22">Peng, Xiaoqun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Changjian%22">Wang, Changjian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tang%2C+Xiaoyan%22">Tang, Xiaoyan</searchLink><relatesTo>2,3,4</relatesTo> (AUTHOR)<i> txy@frontier-ag.com</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Molecular+Breeding%22">Molecular Breeding</searchLink>. May2024, Vol. 44 Issue 5, p1-14. 14p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22MALE+sterility+in+plants%22">MALE sterility in plants</searchLink><br />*<searchLink fieldCode="DE" term="%22HYBRID+rice%22">HYBRID rice</searchLink><br />*<searchLink fieldCode="DE" term="%22SEED+technology%22">SEED technology</searchLink><br />*<searchLink fieldCode="DE" term="%22SIGNAL+peptides%22">SIGNAL peptides</searchLink><br />*<searchLink fieldCode="DE" term="%22RICE%22">RICE</searchLink><br />*<searchLink fieldCode="DE" term="%22PEPTIDES%22">PEPTIDES</searchLink><br />*<searchLink fieldCode="DE" term="%22CARRIER+proteins%22">CARRIER proteins</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Hybrid seed production technology (SPT) is achieved through the utilization of a recessive nuclear male-sterile mutant transformed with a transgenic cassette comprising three essential components: the wild-type gene to restore the fertility of the male-sterile mutant, an α-amylase gene to disrupt transgenic pollen grains, and red fluorescence protein gene DsRed to distinguish the transgenic seeds from the nontransgenic male sterile seeds. In rice, we establish the pollen disruption system by introducing an amyloplast targeting signal peptide (ASP) at the N-terminus of maize α-amylase protein ZM-AA1ΔSP (ZM-AA1 with the N-terminal signal peptide removed). The ASP facilitates the transport of ZM-AA1ΔSP protein into amyloplast where it degrades starch, resulting in disruption of the pollen fertility. To obtain such signal peptides for rice, we searched the rice proteins homologous to the defined wheat amyloplast proteins followed by protein–protein interaction network predictions and targeting signal peptides prediction. These analyses enabled the identification of four candidate ASPs in rice, which were designated as ASP1, ASP2, ASP3, and ASP4, respectively. ASP1 and ASP2, when linked with ZM-AA1ΔSP, exhibited the capability to disrupt transgenic pollen grains, whereas ASP3 and ASP4 did not produce this effect. Interestingly, the localization experiments showed that ASP3 and ASP4 were able to target the proteins into chloroplast. The ASP1 and ASP2 sequences provide valuable tools for genetic engineering of the rice male-sterile system, which will contribute to the hybrid rice breeding and production. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Molecular Breeding is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=a9h&AN=177816616
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s11032-024-01471-y
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 1
    Subjects:
      – SubjectFull: MALE sterility in plants
        Type: general
      – SubjectFull: HYBRID rice
        Type: general
      – SubjectFull: SEED technology
        Type: general
      – SubjectFull: SIGNAL peptides
        Type: general
      – SubjectFull: RICE
        Type: general
      – SubjectFull: PEPTIDES
        Type: general
      – SubjectFull: CARRIER proteins
        Type: general
    Titles:
      – TitleFull: Identification of two plastid transit peptides for construction of pollen-inactivation system in rice.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Wang, Menglong
      – PersonEntity:
          Name:
            NameFull: Peng, Xiaoqun
      – PersonEntity:
          Name:
            NameFull: Wang, Changjian
      – PersonEntity:
          Name:
            NameFull: Tang, Xiaoyan
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Text: May2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 13803743
          Numbering:
            – Type: volume
              Value: 44
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: Molecular Breeding
              Type: main
ResultId 1