A study on assessing the toxic effects of ethyl paraben on rohu (Labeo rohita) using different biomarkers; hemato-biochemical assays, histology, oxidant and antioxidant activity and genotoxicity.
| Title: | A study on assessing the toxic effects of ethyl paraben on rohu (Labeo rohita) using different biomarkers; hemato-biochemical assays, histology, oxidant and antioxidant activity and genotoxicity. |
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| Authors: | Akmal, Hasnain1 (AUTHOR), Ahmad, Shabbir1 (AUTHOR), Abbasi, Muddasir Hassan1 (AUTHOR), Jabeen, Farhat2 (AUTHOR), Shahzad, Khurram1 (AUTHOR) dr.khurram@uo.edu.pk |
| Source: | PLoS ONE. 5/6/2024, Vol. 19 Issue 5, p1-18. 18p. |
| Subject Terms: | *POISONS, *GENETIC toxicology, *ROHU, *BIOMARKERS, *EMERGING contaminants, *ALANINE aminotransferase, *ASPARTATE aminotransferase |
| Abstract: | Parabens are being used as preservatives due to their antifungal and antimicrobial effects. They are emerging as aquatic pollutants due to their excessive use in many products. The purpose of this study was to determine the toxic effect of ethyl paraben (C9H10O3) on the hematobiochemical, histological, oxidative, and anti-oxidant enzymatic and non-enzymatic activity; the study also evaluates the potential of ethyl paraben to cause genotoxicity in Rohu Labeo rohita. A number of 15 fish with an average weight of 35.45±1.34g were placed in each group and exposed to ethyl paraben for 21 days. Three different concentrations of ethyl paraben, i.e., T1 (2000μg/L), T2 (4000 μg/L), andT3 (6000 μg/L) on which fish were exposed as compared to the control T0 (0.00 μg/L). Blood was used for hematobiochemical and comet assay. Gills, kidneys, and liver were removed for histological alterations. The results showed a significant rise in all hemato-biochemical parameters such as RBCs, WBCs, PLT count, blood sugar, albumin, globulin, and cholesterol. An increase in aspartate aminotransferase (AST) and alanine transaminase (ALT) levels directed the hepatocytic damage. Histological alterations in the liver, gills and kidneys of fish were found. Ethylparaben induces oxidative stress by suppressing antioxidant enzyme activity such as SOD, GSH, CAT and POD. Based on the comet assay, DNA damage was also observed in blood cells, resulting in genotoxicity. Findings from the present study indicate that ethyl paraben induces hemato-biochemical alterations, tissue damage, oxidative stress, and genotoxicity. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: A study on assessing the toxic effects of ethyl paraben on rohu (Labeo rohita) using different biomarkers; hemato-biochemical assays, histology, oxidant and antioxidant activity and genotoxicity. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Akmal%2C+Hasnain%22">Akmal, Hasnain</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ahmad%2C+Shabbir%22">Ahmad, Shabbir</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Abbasi%2C+Muddasir+Hassan%22">Abbasi, Muddasir Hassan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jabeen%2C+Farhat%22">Jabeen, Farhat</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shahzad%2C+Khurram%22">Shahzad, Khurram</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> dr.khurram@uo.edu.pk</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22PLoS+ONE%22">PLoS ONE</searchLink>. 5/6/2024, Vol. 19 Issue 5, p1-18. 18p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22POISONS%22">POISONS</searchLink><br />*<searchLink fieldCode="DE" term="%22GENETIC+toxicology%22">GENETIC toxicology</searchLink><br />*<searchLink fieldCode="DE" term="%22ROHU%22">ROHU</searchLink><br />*<searchLink fieldCode="DE" term="%22BIOMARKERS%22">BIOMARKERS</searchLink><br />*<searchLink fieldCode="DE" term="%22EMERGING+contaminants%22">EMERGING contaminants</searchLink><br />*<searchLink fieldCode="DE" term="%22ALANINE+aminotransferase%22">ALANINE aminotransferase</searchLink><br />*<searchLink fieldCode="DE" term="%22ASPARTATE+aminotransferase%22">ASPARTATE aminotransferase</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Parabens are being used as preservatives due to their antifungal and antimicrobial effects. They are emerging as aquatic pollutants due to their excessive use in many products. The purpose of this study was to determine the toxic effect of ethyl paraben (C9H10O3) on the hematobiochemical, histological, oxidative, and anti-oxidant enzymatic and non-enzymatic activity; the study also evaluates the potential of ethyl paraben to cause genotoxicity in Rohu Labeo rohita. A number of 15 fish with an average weight of 35.45±1.34g were placed in each group and exposed to ethyl paraben for 21 days. Three different concentrations of ethyl paraben, i.e., T1 (2000μg/L), T2 (4000 μg/L), andT3 (6000 μg/L) on which fish were exposed as compared to the control T0 (0.00 μg/L). Blood was used for hematobiochemical and comet assay. Gills, kidneys, and liver were removed for histological alterations. The results showed a significant rise in all hemato-biochemical parameters such as RBCs, WBCs, PLT count, blood sugar, albumin, globulin, and cholesterol. An increase in aspartate aminotransferase (AST) and alanine transaminase (ALT) levels directed the hepatocytic damage. Histological alterations in the liver, gills and kidneys of fish were found. Ethylparaben induces oxidative stress by suppressing antioxidant enzyme activity such as SOD, GSH, CAT and POD. Based on the comet assay, DNA damage was also observed in blood cells, resulting in genotoxicity. Findings from the present study indicate that ethyl paraben induces hemato-biochemical alterations, tissue damage, oxidative stress, and genotoxicity. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1371/journal.pone.0302691 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 18 StartPage: 1 Subjects: – SubjectFull: POISONS Type: general – SubjectFull: GENETIC toxicology Type: general – SubjectFull: ROHU Type: general – SubjectFull: BIOMARKERS Type: general – SubjectFull: EMERGING contaminants Type: general – SubjectFull: ALANINE aminotransferase Type: general – SubjectFull: ASPARTATE aminotransferase Type: general Titles: – TitleFull: A study on assessing the toxic effects of ethyl paraben on rohu (Labeo rohita) using different biomarkers; hemato-biochemical assays, histology, oxidant and antioxidant activity and genotoxicity. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Akmal, Hasnain – PersonEntity: Name: NameFull: Ahmad, Shabbir – PersonEntity: Name: NameFull: Abbasi, Muddasir Hassan – PersonEntity: Name: NameFull: Jabeen, Farhat – PersonEntity: Name: NameFull: Shahzad, Khurram IsPartOfRelationships: – BibEntity: Dates: – D: 06 M: 05 Text: 5/6/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 19326203 Numbering: – Type: volume Value: 19 – Type: issue Value: 5 Titles: – TitleFull: PLoS ONE Type: main |
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