Two Steps in Maf1-dependent Repression of Transcription by RNA Polymerase III.
| Title: | Two Steps in Maf1-dependent Repression of Transcription by RNA Polymerase III. |
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| Authors: | Desai, Neelam1, Jaehoon Lee1, Upadhya, Rajendra1, Yaya Chu1, Moir, Robyn D.1, Willis, Tan M.1 willis@aecom.yu.edu |
| Source: | Journal of Biological Chemistry. 2/25/2005, Vol. 280 Issue 8, p6455-6462. 8p. 1 Graph. |
| Subject Terms: | *SACCHAROMYCES cerevisiae, *GENETIC transcription, *CELLULAR signal transduction, *PHYSIOLOGICAL control systems, *RNA polymerases, *NUCLEIC acids |
| Abstract: | In Saccharomyces cerevisiae, Maf1 is essential for mediating the repression of transcription by RNA polymerase (pol) III in response to diverse cellular conditions. These conditions activate distinct signaling pathways that converge at or above Maf1. Thus, Maf1-dependent repression is thought to involve a common set of downstream inhibitory effects on the pol III machinery. Here we provide support for this view and define two steps in Maf1-dependent transcriptional repression. We show that chlorpromazine (CPZ)-induced repression of pol III transcription is achieved by inhibiting de novo assembly of transcription factor (TF) IIIB onto DNA as well as the recruitment of pol Ill to preassembled TFIIIB-DNA complexes. Additionally Brf1 was identified as a target of repression in extracts of CPZ-treated cells. Maf1-Brf1 and Maf1-pol III interactions were implicated in the inhibition of TFIIIB-DNA complex assembly and polymerase recruitment by recombinant Maf1. Co-immunoprecipitation experiments confirmed these interactions in yeast extracts and demonstrated that Mail does not differentially sequester Brf1 or pol Ill under repressing conditions. The results suggest that Mail functions by a non-stoichiometric mechanism to repress pol III transcription. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Two Steps in Maf1-dependent Repression of Transcription by RNA Polymerase III. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Desai%2C+Neelam%22">Desai, Neelam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jaehoon+Lee%22">Jaehoon Lee</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Upadhya%2C+Rajendra%22">Upadhya, Rajendra</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yaya+Chu%22">Yaya Chu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Moir%2C+Robyn+D%2E%22">Moir, Robyn D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Willis%2C+Tan+M%2E%22">Willis, Tan M.</searchLink><relatesTo>1</relatesTo><i> willis@aecom.yu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 2/25/2005, Vol. 280 Issue 8, p6455-6462. 8p. 1 Graph. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22SACCHAROMYCES+cerevisiae%22">SACCHAROMYCES cerevisiae</searchLink><br />*<searchLink fieldCode="DE" term="%22GENETIC+transcription%22">GENETIC transcription</searchLink><br />*<searchLink fieldCode="DE" term="%22CELLULAR+signal+transduction%22">CELLULAR signal transduction</searchLink><br />*<searchLink fieldCode="DE" term="%22PHYSIOLOGICAL+control+systems%22">PHYSIOLOGICAL control systems</searchLink><br />*<searchLink fieldCode="DE" term="%22RNA+polymerases%22">RNA polymerases</searchLink><br />*<searchLink fieldCode="DE" term="%22NUCLEIC+acids%22">NUCLEIC acids</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In Saccharomyces cerevisiae, Maf1 is essential for mediating the repression of transcription by RNA polymerase (pol) III in response to diverse cellular conditions. These conditions activate distinct signaling pathways that converge at or above Maf1. Thus, Maf1-dependent repression is thought to involve a common set of downstream inhibitory effects on the pol III machinery. Here we provide support for this view and define two steps in Maf1-dependent transcriptional repression. We show that chlorpromazine (CPZ)-induced repression of pol III transcription is achieved by inhibiting de novo assembly of transcription factor (TF) IIIB onto DNA as well as the recruitment of pol Ill to preassembled TFIIIB-DNA complexes. Additionally Brf1 was identified as a target of repression in extracts of CPZ-treated cells. Maf1-Brf1 and Maf1-pol III interactions were implicated in the inhibition of TFIIIB-DNA complex assembly and polymerase recruitment by recombinant Maf1. Co-immunoprecipitation experiments confirmed these interactions in yeast extracts and demonstrated that Mail does not differentially sequester Brf1 or pol Ill under repressing conditions. The results suggest that Mail functions by a non-stoichiometric mechanism to repress pol III transcription. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1074/jbc.M412375200 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 6455 Subjects: – SubjectFull: SACCHAROMYCES cerevisiae Type: general – SubjectFull: GENETIC transcription Type: general – SubjectFull: CELLULAR signal transduction Type: general – SubjectFull: PHYSIOLOGICAL control systems Type: general – SubjectFull: RNA polymerases Type: general – SubjectFull: NUCLEIC acids Type: general Titles: – TitleFull: Two Steps in Maf1-dependent Repression of Transcription by RNA Polymerase III. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Desai, Neelam – PersonEntity: Name: NameFull: Jaehoon Lee – PersonEntity: Name: NameFull: Upadhya, Rajendra – PersonEntity: Name: NameFull: Yaya Chu – PersonEntity: Name: NameFull: Moir, Robyn D. – PersonEntity: Name: NameFull: Willis, Tan M. IsPartOfRelationships: – BibEntity: Dates: – D: 25 M: 02 Text: 2/25/2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 00219258 Numbering: – Type: volume Value: 280 – Type: issue Value: 8 Titles: – TitleFull: Journal of Biological Chemistry Type: main |
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