Determination of Oxidative Stress and Antioxidant Activities in Dogs Infected with Canine Distemper Virus.

Bibliographic Details
Title: Determination of Oxidative Stress and Antioxidant Activities in Dogs Infected with Canine Distemper Virus.
Alternate Title: Canine Distemper Virüs Enfeksiyonu Bulunan Köpeklerde Oksidatif Stres ve Antioksidan Aktivitelerin Belirlenmesi.
Authors: HATİPOĞLU, Durmuş1 drhatip@selcuk.edu.tr, DİK, Irmak2, GÜLERSOY, Erdem3
Source: Van Veterinary Journal. Nov2022, Vol. 33 Issue 3, p67-70. 4p.
Subject Terms: *CANINE distemper virus, *LIPID peroxidation (Biology), *DOG breeds, *OXIDATIVE stress, *DOGS, *ENZYME-linked immunosorbent assay, *REACTIVE oxygen species
Abstract (English): Supraphysiological reactive oxygen species (ROS) has been linked to a variety of diseases, including cancer, inflammation, and neurodegeneration. One of the diseases pathophysiologically associated with ROS accumulation is canine distemper (CD). The purpose of this study was to compare lipid peroxidation and antioxidant biomarkers in dogs infected with canine distemper virus (CDV) to healthy dogs. The animals in this study consisted of a total of 20 dogs, 10 CDV-positive dogs (Trial group) aged 0-12 months of different breeds and sexes, and 10 healthy dogs (Control group) aged 0-12 months of different breeds and sexes. Thiobarbituric acid reactive substances (TBARS), glutathione (GSH) and superoxide dismutase (SOD) were evaluated with commercially available enzyme-linked immunosorbent assay kits using serum samples. While the activities of GSH and SOD were statistically lower in CDV positive animals compared to healthy animals (p<0.05), the level of TBARS was significantly higher (p<0.05). In conclusion, current study findings that CDV increases lipid peroxidation activity in serum while decreasing antioxidant enzyme levels may be useful for future research. [ABSTRACT FROM AUTHOR]
Abstract (Turkish): Suprafizyolojik reaktif oksijen türleri (ROS), kanser, inflamasyon ve nörodejenerasyon dahil olmak üzere çeşitli hastalıklarla ilişkilidir. Patofizyolojik olarak ROS birikimi ile ilişkili diğer hastalıklardan biri de köpek distemperi'dir (CD). Bu çalışmanın amacı, canine distemper virüsü (CDV) ile enfekte köpeklerde lipid peroksidasyonu ve antioksidan biyobelirteçleri sağlıklı köpeklerle karşılaştırmaktı. Bu çalışmadaki hayvanlar, 0-12 aylık farklı ırk ve cinsiyetlerden 10 CDV pozitif köpek (Deneme grubu) ve 0-12 aylık farklı ırk ve cinsiyetlerden 10 sağlıklı köpek (Kontrol grubu) olmak üzere toplam 20 köpekten oluşmaktadır. Tiyobarbitürik asit reaktif maddeler (TBARS), glutatyon (GSH) ve süperoksit dismutaz (SOD) seviyeleri serumdan ticari olarak temin edilebilen enzim bağlantılı immünosorbent tahlil kitleri ile değerlendirildi. GSH ve SOD aktiviteleri, CDV pozitif hayvanlarda sağlıklı hayvanlara kıyasla istatistiksel olarak daha düşük iken (p<0.05), TBARS seviyesi önemli ölçüde daha yüksekti (p<0.05). Sonuç olarak, CDV'nin serumdaki lipid peroksidasyon aktivitesini artırırken antioksidan enzim düzeylerini azalttığına dair mevcut çalışma bulguları gelecekteki araştırmalar için faydalı olabilir. [ABSTRACT FROM AUTHOR]
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Database: Academic Search Complete
More Details
ISSN:21493359
DOI:10.36483/vanvetj.1136569
Published in:Van Veterinary Journal
Language:English