Bibliographic Details
| Title: |
A ratiometric fluorescent nanoprobe for ultrafast imaging of peroxynitrite in living cells. |
| Authors: |
Cui, Yijing1 (AUTHOR), Han, Suping2 (AUTHOR), Zhang, Jingjing1 (AUTHOR), Wang, Xiaoqing1,3 (AUTHOR) xqwang@njfu.edu.cn |
| Source: |
Journal of Biological Inorganic Chemistry (JBIC). Sep2022, Vol. 27 Issue 6, p595-603. 9p. |
| Subject Terms: |
*PEROXYNITRITE, *BLOCK copolymers, *COUMARINS, *CHEMICAL biology, *FLUORESCENT probes, *CELL imaging, *POLYMERSOMES |
| Abstract: |
For ratiometrically imaging peroxynitrite (ONOO−) in living cells, we devised and fabricated a novel fluorescent nanoprobe, NC-NP530/460, in this study. To achieve ratiometric fluorescence response towards ONOO−, NC-NP530/460 used 3-(2-benzothiazolyl) coumarin (Cou-Bz) as the internal reference and 1,8-naphthimide derivative (Naph-PN) as a fluorescent ONOO− probe. These compounds were incorporated into an amphiphilic block polymer called Pluronic F-127. In addition to an ultrafast response to ONOO−, NC-NP530/460 also showed great selectivity and sensitive detection (detection limit was 4.51 μM). It was important to note that NC-NP530/460 demonstrated solid performance for ONOO− fluorescence ratio imaging in living cells, highlighting its potential for ONOO−-related chemical biology research. [ABSTRACT FROM AUTHOR] |
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