Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse.
| Title: | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse. |
|---|---|
| Authors: | Credendino, Sara Carmela1, Lewin, Nicole1, de Oliveira, Miriane1,2, Basu, Swaraj3, D’Andrea, Barbara1, Amendola, Elena1, Di Guida, Luigi1, Nardone, Antonio4, Sanges, Remo5, De Felice, Mario1, De Vita, Gabriella1 |
| Source: | International Journal of Genomics. 9/10/2017, p1-7. 7p. |
| Subject Terms: | *THYROID gland, *ANTISENSE peptides, *RNA splicing, *GENE expression, *GENOMICS |
| Abstract: | lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described. Klhl14-AS is expressed with a peculiar pattern, characterized by diverse relative abundance of its isoforms in different mouse tissues. We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. Interestingly, databases report a similar expression profile for human Klhl14-AS. Our observations suggest that this lncRNA could play cell type-specific roles in several organs and pave the way for functional characterization of this gene in appropriate biological contexts. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Credendino%2C+Sara+Carmela%22">Credendino, Sara Carmela</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lewin%2C+Nicole%22">Lewin, Nicole</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22de+Oliveira%2C+Miriane%22">de Oliveira, Miriane</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Basu%2C+Swaraj%22">Basu, Swaraj</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22D’Andrea%2C+Barbara%22">D’Andrea, Barbara</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Amendola%2C+Elena%22">Amendola, Elena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Di+Guida%2C+Luigi%22">Di Guida, Luigi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nardone%2C+Antonio%22">Nardone, Antonio</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Sanges%2C+Remo%22">Sanges, Remo</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Felice%2C+Mario%22">De Felice, Mario</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Vita%2C+Gabriella%22">De Vita, Gabriella</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Genomics%22">International Journal of Genomics</searchLink>. 9/10/2017, p1-7. 7p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22THYROID+gland%22">THYROID gland</searchLink><br />*<searchLink fieldCode="DE" term="%22ANTISENSE+peptides%22">ANTISENSE peptides</searchLink><br />*<searchLink fieldCode="DE" term="%22RNA+splicing%22">RNA splicing</searchLink><br />*<searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br />*<searchLink fieldCode="DE" term="%22GENOMICS%22">GENOMICS</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described. Klhl14-AS is expressed with a peculiar pattern, characterized by diverse relative abundance of its isoforms in different mouse tissues. We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. Interestingly, databases report a similar expression profile for human Klhl14-AS. Our observations suggest that this lncRNA could play cell type-specific roles in several organs and pave the way for functional characterization of this gene in appropriate biological contexts. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of International Journal of Genomics is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1155/2017/9769171 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 1 Subjects: – SubjectFull: THYROID gland Type: general – SubjectFull: ANTISENSE peptides Type: general – SubjectFull: RNA splicing Type: general – SubjectFull: GENE expression Type: general – SubjectFull: GENOMICS Type: general Titles: – TitleFull: Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Credendino, Sara Carmela – PersonEntity: Name: NameFull: Lewin, Nicole – PersonEntity: Name: NameFull: de Oliveira, Miriane – PersonEntity: Name: NameFull: Basu, Swaraj – PersonEntity: Name: NameFull: D’Andrea, Barbara – PersonEntity: Name: NameFull: Amendola, Elena – PersonEntity: Name: NameFull: Di Guida, Luigi – PersonEntity: Name: NameFull: Nardone, Antonio – PersonEntity: Name: NameFull: Sanges, Remo – PersonEntity: Name: NameFull: De Felice, Mario – PersonEntity: Name: NameFull: De Vita, Gabriella IsPartOfRelationships: – BibEntity: Dates: – D: 10 M: 09 Text: 9/10/2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 2314436X Titles: – TitleFull: International Journal of Genomics Type: main |
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