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The antenna transcriptome changes in mosquito Anopheles sinensis, pre- and post- blood meal.

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Title: The antenna transcriptome changes in mosquito Anopheles sinensis, pre- and post- blood meal.
Authors: Chen, Qian1, Pei, Di1, Li, Jianyong1, Jing, Chengyu1, Man, Yahui1, Wu, Wenjian1,2
Source: PLoS ONE. 7/17/2017, Vol. 12 Issue 7, p1-14. 14p.
Subject Terms: *MOSQUITOES, *BLOOD meal as feed, *ANTENNAE (Biology), *CHEMICAL senses, *ANIMAL behavior, MALARIA transmission
Abstract: Antenna is the main chemosensory organ in mosquitoes. Characterization of the transcriptional changes after blood meal, especially those related to chemoreception, may help to explain mosquito blood sucking behavior and to identify novel targets for mosquito control. Anopheles sinensis is an Asiatic mosquito species which transmits malaria and lymphatic filariasis. However, studies on chemosensory biology in female An. sinensis are quite lacking. Here we report a transcriptome analysis of An. sinensis female antennae pre- and post- blood meal. We created six An. sinensis antenna RNA-seq libraries, three from females without blood meal and three from females five hours after a blood meal. Illumina sequencing was conducted to analyze the transcriptome differences between the two groups. In total, the sequenced fragments created 21,643 genes, 1,828 of them were novel. 12,861 of these genes were considered to be expressed (FPKM >1.0) in at least one of the two groups, with 12,159 genes expressed in both groups. 548 genes were differentially expressed in the blood-fed group, with 331 genes up-regulated and 217 genes down-regulated. GO enrichment analysis of the differentially expressed genes suggested that there were no statistically over represented GO terms among down-regulated genes in blood-fed mosquitoes, while the enriched GO terms of the up-regulated genes occurred mainly in metabolic process. For the chemosensory gene families, a subtle distinction in the expression levels can be observed according to our statistical analysis. However, the firstly comprehensive identification of these chemosensory gene families in An. sinensis antennae will help to characterize the precise function of these proteins in odor recognition in mosquitoes. This study provides a first global view in the changes of transcript accumulation elicited by blood meal in An. sinensis female antennae. [ABSTRACT FROM AUTHOR]
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: The antenna transcriptome changes in mosquito Anopheles sinensis, pre- and post- blood meal.
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  Data: <searchLink fieldCode="AR" term="%22Chen%2C+Qian%22">Chen, Qian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pei%2C+Di%22">Pei, Di</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Jianyong%22">Li, Jianyong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jing%2C+Chengyu%22">Jing, Chengyu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Man%2C+Yahui%22">Man, Yahui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Wenjian%22">Wu, Wenjian</searchLink><relatesTo>1,2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22PLoS+ONE%22">PLoS ONE</searchLink>. 7/17/2017, Vol. 12 Issue 7, p1-14. 14p.
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  Data: *<searchLink fieldCode="DE" term="%22MOSQUITOES%22">MOSQUITOES</searchLink><br />*<searchLink fieldCode="DE" term="%22BLOOD+meal+as+feed%22">BLOOD meal as feed</searchLink><br />*<searchLink fieldCode="DE" term="%22ANTENNAE+%28Biology%29%22">ANTENNAE (Biology)</searchLink><br />*<searchLink fieldCode="DE" term="%22CHEMICAL+senses%22">CHEMICAL senses</searchLink><br />*<searchLink fieldCode="DE" term="%22ANIMAL+behavior%22">ANIMAL behavior</searchLink><br /><searchLink fieldCode="DE" term="%22MALARIA+transmission%22">MALARIA transmission</searchLink>
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  Label: Abstract
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  Data: Antenna is the main chemosensory organ in mosquitoes. Characterization of the transcriptional changes after blood meal, especially those related to chemoreception, may help to explain mosquito blood sucking behavior and to identify novel targets for mosquito control. Anopheles sinensis is an Asiatic mosquito species which transmits malaria and lymphatic filariasis. However, studies on chemosensory biology in female An. sinensis are quite lacking. Here we report a transcriptome analysis of An. sinensis female antennae pre- and post- blood meal. We created six An. sinensis antenna RNA-seq libraries, three from females without blood meal and three from females five hours after a blood meal. Illumina sequencing was conducted to analyze the transcriptome differences between the two groups. In total, the sequenced fragments created 21,643 genes, 1,828 of them were novel. 12,861 of these genes were considered to be expressed (FPKM >1.0) in at least one of the two groups, with 12,159 genes expressed in both groups. 548 genes were differentially expressed in the blood-fed group, with 331 genes up-regulated and 217 genes down-regulated. GO enrichment analysis of the differentially expressed genes suggested that there were no statistically over represented GO terms among down-regulated genes in blood-fed mosquitoes, while the enriched GO terms of the up-regulated genes occurred mainly in metabolic process. For the chemosensory gene families, a subtle distinction in the expression levels can be observed according to our statistical analysis. However, the firstly comprehensive identification of these chemosensory gene families in An. sinensis antennae will help to characterize the precise function of these proteins in odor recognition in mosquitoes. This study provides a first global view in the changes of transcript accumulation elicited by blood meal in An. sinensis female antennae. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1371/journal.pone.0181399
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        Text: English
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      – SubjectFull: MALARIA transmission
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              Text: 7/17/2017
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