Antiplatelet and antiproliferative action of disintegrin from Echis multisquamatis snake venom.
| Title: | Antiplatelet and antiproliferative action of disintegrin from Echis multisquamatis snake venom. |
|---|---|
| Authors: | Chernyshenko, Volodymyr1 bio.cherv@gmail.com, Petruk, Natalia2, Korolova, Darya1, Kasatkina, Ludmila1, Gornytska, Olha1, Platonova, Tetyana1, Chernyshenko, Tamara1, Rebriev, Andriy1, Dzhus, Olena2, Garmanchuk, Liudmyla2, Lugovskoy, Eduard1 |
| Source: | Croatian Medical Journal. Apr2017, Vol. 58 Issue 2, p118-127. 10p. |
| Subject Terms: | *PLATELET aggregation inhibitors, *DISINTEGRINS, *PEPTIDES, *SNAKE venom, *ELECTROPHORESIS, *THERAPEUTICS |
| Abstract: | Aim To purify the platelet aggregation inhibitor from Echis multisquamatis snake venom (PAIEM) and characterize its effect on platelet aggregation and HeLa cell proliferation. Methods Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix assisted laser desorption/ ionization time-of-flight (MALDI-TOF) were used for PAIEM identification. Platelet aggregation in the presence of PAIEM was studied on aggregometer Solar-AP2110. The changes of shape and granularity of platelets in the presence of PAIEM were studied on flow cytometer COULTER EPICS XL, and degranulation of platelets was estimated using spectrofluorimetry. Indirect enzyme-linked immunosorbent assay was used for the determination of target of PAIEM on platelet surface. An assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to evaluate the effect of PAIEM on the proliferation of HeLa cells in cell culture. Results The molecular weight of the protein purified from Echis multisquamatis venom was 14.9 kDa. Half-maximal inhibitory concentration (IC50) of PAIEM needed to inhibit adenosine diphosphate (ADP)-induced platelet aggregation was 7 μM. PAIEM did not affect thrombin- or ADP-induced platelet activation, but it did prevent binding of the anti-IIb antibody to glycoprotein IIb/IIIa (GPIIbIIIa)-receptor of adhered platelets and inhibited the viability of HeLa cells by 54%. Conclusion As a member of the disintegrin family, PAIEM inhibited platelet aggregation and cell proliferation possibly by blocking integrin-mediated interactions. However, it did not impair cellular signaling causing any changes in platelet shape and granularity and did not affect ADP-induced platelet degranulation. This disintegrin was shown to be a potent inhibitor of integrin-mediated cellular interactions including platelet aggregation or cancer cell proliferation. [ABSTRACT FROM AUTHOR] |
| Copyright of Croatian Medical Journal is the property of Croatian Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Academic Search Complete |
| FullText | Links: – Type: pdflink Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:a9h&genre=article&issn=03539504&ISBN=&volume=58&issue=2&date=20170401&spage=118&pages=118-127&title=Croatian Medical Journal&atitle=Antiplatelet%20and%20antiproliferative%20action%20of%20disintegrin%20from%20Echis%20multisquamatis%20snake%20venom.&aulast=Chernyshenko%2C%20Volodymyr&id=DOI:10.3325/cmj.2017.58.118 Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: a9h DbLabel: Academic Search Complete An: 122612962 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Antiplatelet and antiproliferative action of disintegrin from Echis multisquamatis snake venom. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chernyshenko%2C+Volodymyr%22">Chernyshenko, Volodymyr</searchLink><relatesTo>1</relatesTo><i> bio.cherv@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Petruk%2C+Natalia%22">Petruk, Natalia</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Korolova%2C+Darya%22">Korolova, Darya</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kasatkina%2C+Ludmila%22">Kasatkina, Ludmila</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gornytska%2C+Olha%22">Gornytska, Olha</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Platonova%2C+Tetyana%22">Platonova, Tetyana</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chernyshenko%2C+Tamara%22">Chernyshenko, Tamara</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rebriev%2C+Andriy%22">Rebriev, Andriy</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Dzhus%2C+Olena%22">Dzhus, Olena</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Garmanchuk%2C+Liudmyla%22">Garmanchuk, Liudmyla</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lugovskoy%2C+Eduard%22">Lugovskoy, Eduard</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Croatian+Medical+Journal%22">Croatian Medical Journal</searchLink>. Apr2017, Vol. 58 Issue 2, p118-127. 10p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22PLATELET+aggregation+inhibitors%22">PLATELET aggregation inhibitors</searchLink><br />*<searchLink fieldCode="DE" term="%22DISINTEGRINS%22">DISINTEGRINS</searchLink><br />*<searchLink fieldCode="DE" term="%22PEPTIDES%22">PEPTIDES</searchLink><br />*<searchLink fieldCode="DE" term="%22SNAKE+venom%22">SNAKE venom</searchLink><br />*<searchLink fieldCode="DE" term="%22ELECTROPHORESIS%22">ELECTROPHORESIS</searchLink><br />*<searchLink fieldCode="DE" term="%22THERAPEUTICS%22">THERAPEUTICS</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aim To purify the platelet aggregation inhibitor from Echis multisquamatis snake venom (PAIEM) and characterize its effect on platelet aggregation and HeLa cell proliferation. Methods Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix assisted laser desorption/ ionization time-of-flight (MALDI-TOF) were used for PAIEM identification. Platelet aggregation in the presence of PAIEM was studied on aggregometer Solar-AP2110. The changes of shape and granularity of platelets in the presence of PAIEM were studied on flow cytometer COULTER EPICS XL, and degranulation of platelets was estimated using spectrofluorimetry. Indirect enzyme-linked immunosorbent assay was used for the determination of target of PAIEM on platelet surface. An assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to evaluate the effect of PAIEM on the proliferation of HeLa cells in cell culture. Results The molecular weight of the protein purified from Echis multisquamatis venom was 14.9 kDa. Half-maximal inhibitory concentration (IC50) of PAIEM needed to inhibit adenosine diphosphate (ADP)-induced platelet aggregation was 7 μM. PAIEM did not affect thrombin- or ADP-induced platelet activation, but it did prevent binding of the anti-IIb antibody to glycoprotein IIb/IIIa (GPIIbIIIa)-receptor of adhered platelets and inhibited the viability of HeLa cells by 54%. Conclusion As a member of the disintegrin family, PAIEM inhibited platelet aggregation and cell proliferation possibly by blocking integrin-mediated interactions. However, it did not impair cellular signaling causing any changes in platelet shape and granularity and did not affect ADP-induced platelet degranulation. This disintegrin was shown to be a potent inhibitor of integrin-mediated cellular interactions including platelet aggregation or cancer cell proliferation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Croatian Medical Journal is the property of Croatian Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=a9h&AN=122612962 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3325/cmj.2017.58.118 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 118 Subjects: – SubjectFull: PLATELET aggregation inhibitors Type: general – SubjectFull: DISINTEGRINS Type: general – SubjectFull: PEPTIDES Type: general – SubjectFull: SNAKE venom Type: general – SubjectFull: ELECTROPHORESIS Type: general – SubjectFull: THERAPEUTICS Type: general Titles: – TitleFull: Antiplatelet and antiproliferative action of disintegrin from Echis multisquamatis snake venom. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chernyshenko, Volodymyr – PersonEntity: Name: NameFull: Petruk, Natalia – PersonEntity: Name: NameFull: Korolova, Darya – PersonEntity: Name: NameFull: Kasatkina, Ludmila – PersonEntity: Name: NameFull: Gornytska, Olha – PersonEntity: Name: NameFull: Platonova, Tetyana – PersonEntity: Name: NameFull: Chernyshenko, Tamara – PersonEntity: Name: NameFull: Rebriev, Andriy – PersonEntity: Name: NameFull: Dzhus, Olena – PersonEntity: Name: NameFull: Garmanchuk, Liudmyla – PersonEntity: Name: NameFull: Lugovskoy, Eduard IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 03539504 Numbering: – Type: volume Value: 58 – Type: issue Value: 2 Titles: – TitleFull: Croatian Medical Journal Type: main |
| ResultId | 1 |