Peroxisome biogenesis: a novel inducible PEX19 splicing variant is involved in early stages of peroxisome proliferation.
| Title: | Peroxisome biogenesis: a novel inducible PEX19 splicing variant is involved in early stages of peroxisome proliferation. |
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| Authors: | Naohiko Kinoshita1, Akira Matsuura2, Yukio Fujiki3 yfujiki@kyudai.jp |
| Source: | Journal of Biochemistry. Mar2017, Vol. 161 Issue 3, p297-308. 12p. |
| Subject Terms: | *PEROXISOMES, *PEROXISOME proliferator-activated receptors, *ISOPRENYLATION, *LIVER regeneration, *MICE physiology, *LABORATORY mice |
| Abstract: | Pex19p harbouring a prenylation CAAX box functions as a chaperone and transporter for peroxisomal membrane proteins in membrane assembly. By functional phenotypecomplementation assay using a pex19 Chinese hamster ovary cell mutant ZP119, we herein cloned a rat cDNA encoding a protein similar to Pex19p, but with a C-terminal hydrophobic segment in place of the CAAX box region. The transcript of this gene was highly induced by treatment of rats with a peroxisome proliferator, clofibrate, hence termed PEX19i, while the other three less prominently inducible PEX19 variants encoded authentic Pex19p but differed in the length of 3' non-coding region. Pex19pi restored peroxisomes in ZP119 with slightly lower efficiency than Pex19p, showing apparently weaker interaction with Pex11pβ essential for peroxisome proliferation. However, the C-terminal region of Pex19p was not essential for the association of Pex19p with peroxisomal membrane and interaction with membrane assembly factors, Pex3p and Pex16p. Non-prenylated Pex19p interacted with a membrane protein cargo, Pex14p, but more weakly than Pex19pi and the farnesylated Pex19p. Thus, PEX19i most likely plays important roles involving the membrane formation at early stages, in prompt response to peroxisome proliferation. Similar types of PEX19 mRNA variants were also elevated in mouse regenerating liver. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Peroxisome biogenesis: a novel inducible PEX19 splicing variant is involved in early stages of peroxisome proliferation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Naohiko+Kinoshita%22">Naohiko Kinoshita</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Akira+Matsuura%22">Akira Matsuura</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Yukio+Fujiki%22">Yukio Fujiki</searchLink><relatesTo>3</relatesTo><i> yfujiki@kyudai.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biochemistry%22">Journal of Biochemistry</searchLink>. Mar2017, Vol. 161 Issue 3, p297-308. 12p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22PEROXISOMES%22">PEROXISOMES</searchLink><br />*<searchLink fieldCode="DE" term="%22PEROXISOME+proliferator-activated+receptors%22">PEROXISOME proliferator-activated receptors</searchLink><br />*<searchLink fieldCode="DE" term="%22ISOPRENYLATION%22">ISOPRENYLATION</searchLink><br />*<searchLink fieldCode="DE" term="%22LIVER+regeneration%22">LIVER regeneration</searchLink><br />*<searchLink fieldCode="DE" term="%22MICE+physiology%22">MICE physiology</searchLink><br />*<searchLink fieldCode="DE" term="%22LABORATORY+mice%22">LABORATORY mice</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Pex19p harbouring a prenylation CAAX box functions as a chaperone and transporter for peroxisomal membrane proteins in membrane assembly. By functional phenotypecomplementation assay using a pex19 Chinese hamster ovary cell mutant ZP119, we herein cloned a rat cDNA encoding a protein similar to Pex19p, but with a C-terminal hydrophobic segment in place of the CAAX box region. The transcript of this gene was highly induced by treatment of rats with a peroxisome proliferator, clofibrate, hence termed PEX19i, while the other three less prominently inducible PEX19 variants encoded authentic Pex19p but differed in the length of 3' non-coding region. Pex19pi restored peroxisomes in ZP119 with slightly lower efficiency than Pex19p, showing apparently weaker interaction with Pex11pβ essential for peroxisome proliferation. However, the C-terminal region of Pex19p was not essential for the association of Pex19p with peroxisomal membrane and interaction with membrane assembly factors, Pex3p and Pex16p. Non-prenylated Pex19p interacted with a membrane protein cargo, Pex14p, but more weakly than Pex19pi and the farnesylated Pex19p. Thus, PEX19i most likely plays important roles involving the membrane formation at early stages, in prompt response to peroxisome proliferation. Similar types of PEX19 mRNA variants were also elevated in mouse regenerating liver. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biochemistry is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1093/jb/mvw075 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 297 Subjects: – SubjectFull: PEROXISOMES Type: general – SubjectFull: PEROXISOME proliferator-activated receptors Type: general – SubjectFull: ISOPRENYLATION Type: general – SubjectFull: LIVER regeneration Type: general – SubjectFull: MICE physiology Type: general – SubjectFull: LABORATORY mice Type: general Titles: – TitleFull: Peroxisome biogenesis: a novel inducible PEX19 splicing variant is involved in early stages of peroxisome proliferation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Naohiko Kinoshita – PersonEntity: Name: NameFull: Akira Matsuura – PersonEntity: Name: NameFull: Yukio Fujiki IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 0021924X Numbering: – Type: volume Value: 161 – Type: issue Value: 3 Titles: – TitleFull: Journal of Biochemistry Type: main |
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