Genotyping from semen of wild Japanese macaques (Macaca fuscata).
| Title: | Genotyping from semen of wild Japanese macaques (Macaca fuscata). |
|---|---|
| Authors: | Xavier Domingo-Roura1, Josep Marmi1, Olga Andrés1, Juichi Yamagiwa2, Jaume Terradas3 |
| Source: | American Journal of Primatology. Jan2004, Vol. 62 Issue 1, p31-42. 12p. |
| Subject Terms: | *ANIMAL diseases, *POPULATION, *GENOTYPE-environment interaction, *DNA |
| Abstract: | The noninvasive collection of animal cells is crucial for DNA analyses in wild populations that cannot be disturbed by capture. We describe the collection of 68 semen samples following copulation and masturbation events in wild habituated and nonhabituated troops of Japanese macaques on the protected island of Yakushima. We used this DNA to amplify 390 base pairs (bp) of the mitochondrial DNA control region in 16 individuals from eight troops, and found a monomorphic pattern in agreement with the low variability imposed by geographic isolation and female philopatry. We also amplified two microsatellite loci from samples collected after the resident males of a focal troop had copulated with different females. We found several different allele combinations in samples collected after the observed mating of a single male, indicating the presence of contaminant DNA, presumably from males that had previously mated with the same female. This discovery made it impossible to assign a given sample to a specific male except when the samples were recovered after masturbation events. Thus, it was not possible to test for kinship or estimate allele frequencies from the semen samples. The mixing of semen, and the pattern of sample collection observed in morphologically identified individuals support the notion that strong mating and sperm competition exists among resident and nonresident males. Am. J. Primatol. 62:3142, 2004. Copyright © 2004 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Genotyping from semen of wild Japanese macaques (Macaca fuscata). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Xavier+Domingo-Roura%22">Xavier Domingo-Roura</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Josep+Marmi%22">Josep Marmi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Olga+Andrés%22">Olga Andrés</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Juichi+Yamagiwa%22">Juichi Yamagiwa</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Jaume+Terradas%22">Jaume Terradas</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Primatology%22">American Journal of Primatology</searchLink>. Jan2004, Vol. 62 Issue 1, p31-42. 12p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22ANIMAL+diseases%22">ANIMAL diseases</searchLink><br />*<searchLink fieldCode="DE" term="%22POPULATION%22">POPULATION</searchLink><br />*<searchLink fieldCode="DE" term="%22GENOTYPE-environment+interaction%22">GENOTYPE-environment interaction</searchLink><br />*<searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The noninvasive collection of animal cells is crucial for DNA analyses in wild populations that cannot be disturbed by capture. We describe the collection of 68 semen samples following copulation and masturbation events in wild habituated and nonhabituated troops of Japanese macaques on the protected island of Yakushima. We used this DNA to amplify 390 base pairs (bp) of the mitochondrial DNA control region in 16 individuals from eight troops, and found a monomorphic pattern in agreement with the low variability imposed by geographic isolation and female philopatry. We also amplified two microsatellite loci from samples collected after the resident males of a focal troop had copulated with different females. We found several different allele combinations in samples collected after the observed mating of a single male, indicating the presence of contaminant DNA, presumably from males that had previously mated with the same female. This discovery made it impossible to assign a given sample to a specific male except when the samples were recovered after masturbation events. Thus, it was not possible to test for kinship or estimate allele frequencies from the semen samples. The mixing of semen, and the pattern of sample collection observed in morphologically identified individuals support the notion that strong mating and sperm competition exists among resident and nonresident males. Am. J. Primatol. 62:3142, 2004. Copyright © 2004 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of American Journal of Primatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/ajp.20001 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 31 Subjects: – SubjectFull: ANIMAL diseases Type: general – SubjectFull: POPULATION Type: general – SubjectFull: GENOTYPE-environment interaction Type: general – SubjectFull: DNA Type: general Titles: – TitleFull: Genotyping from semen of wild Japanese macaques (Macaca fuscata). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Xavier Domingo-Roura – PersonEntity: Name: NameFull: Josep Marmi – PersonEntity: Name: NameFull: Olga Andrés – PersonEntity: Name: NameFull: Juichi Yamagiwa – PersonEntity: Name: NameFull: Jaume Terradas IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2004 Type: published Y: 2004 Identifiers: – Type: issn-print Value: 02752565 Numbering: – Type: volume Value: 62 – Type: issue Value: 1 Titles: – TitleFull: American Journal of Primatology Type: main |
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