Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody.
| Title: | Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody. |
|---|---|
| Authors: | Kuen-Phon Wu, Maria Silvia1, Chih-Wei Wu, Maria Silvia1, Ya-Ping Tsao1, Ting-Wei Kuo, Maria Silvia1, Yuan-Chao Lou1, Cheng-Wen Lin1, Suh-Chin Wu1, Jya-Wei Cheng1 jwcheng@life.nthu.edu.tw |
| Source: | Journal of Biological Chemistry. 11/14/2003, Vol. 278 Issue 46, p46007-46013. 7p. 3 Diagrams, 1 Chart, 1 Graph. |
| Subject Terms: | *FLAVIVIRUSES, *PROTEINS, *ANTIGENS |
| Abstract: | The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a β-barrel type structure composed of six antiparallel β-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses. [ABSTRACT FROM AUTHOR] |
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| Database: | Academic Search Complete |
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| Items | – Name: Title Label: Title Group: Ti Data: Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kuen-Phon+Wu%2C+Maria+Silvia%22">Kuen-Phon Wu, Maria Silvia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chih-Wei+Wu%2C+Maria+Silvia%22">Chih-Wei Wu, Maria Silvia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ya-Ping+Tsao%22">Ya-Ping Tsao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ting-Wei+Kuo%2C+Maria+Silvia%22">Ting-Wei Kuo, Maria Silvia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yuan-Chao+Lou%22">Yuan-Chao Lou</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cheng-Wen+Lin%22">Cheng-Wen Lin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Suh-Chin+Wu%22">Suh-Chin Wu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jya-Wei+Cheng%22">Jya-Wei Cheng</searchLink><relatesTo>1</relatesTo><i> jwcheng@life.nthu.edu.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 11/14/2003, Vol. 278 Issue 46, p46007-46013. 7p. 3 Diagrams, 1 Chart, 1 Graph. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22FLAVIVIRUSES%22">FLAVIVIRUSES</searchLink><br />*<searchLink fieldCode="DE" term="%22PROTEINS%22">PROTEINS</searchLink><br />*<searchLink fieldCode="DE" term="%22ANTIGENS%22">ANTIGENS</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a β-barrel type structure composed of six antiparallel β-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1074/jbc.M307776200 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 46007 Subjects: – SubjectFull: FLAVIVIRUSES Type: general – SubjectFull: PROTEINS Type: general – SubjectFull: ANTIGENS Type: general Titles: – TitleFull: Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kuen-Phon Wu, Maria Silvia – PersonEntity: Name: NameFull: Chih-Wei Wu, Maria Silvia – PersonEntity: Name: NameFull: Ya-Ping Tsao – PersonEntity: Name: NameFull: Ting-Wei Kuo, Maria Silvia – PersonEntity: Name: NameFull: Yuan-Chao Lou – PersonEntity: Name: NameFull: Cheng-Wen Lin – PersonEntity: Name: NameFull: Suh-Chin Wu – PersonEntity: Name: NameFull: Jya-Wei Cheng IsPartOfRelationships: – BibEntity: Dates: – D: 14 M: 11 Text: 11/14/2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 00219258 Numbering: – Type: volume Value: 278 – Type: issue Value: 46 Titles: – TitleFull: Journal of Biological Chemistry Type: main |
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