A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway.
| Title: | A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway. |
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| Authors: | Eckwahl, Matthew J.1, Soyeong Sim1, Smith, Derek2, Telesnitsky, Alice3, Wolin, Sandra L.1,2,4 sandra.wolin@yale.edu |
| Source: | Genes & Development. 2015, Vol. 29 Issue 6, p646-657. 12p. |
| Subject Terms: | *MOLONEY murine leukemia virus, *RETROVIRUSES, *LEUKEMIA, *SMALL nuclear RNA, *LABORATORY mice |
| Abstract: | Although all retroviruses recruit host cell RNAs into virions, both the spectrum of RNAs encapsidated and the mechanisms by which they are recruited remain largely unknown. Here, we used high-throughput sequencing to obtain a comprehensive description of the RNAs packaged by a model retrovirus, murine leukemia virus. The major encapsidated host RNAs are noncoding RNAs (ncRNAs) and members of the VL30 class of endogenous retroviruses. Remarkably, although Moloney leukemia virus (MLV) assembles in the cytoplasm, precursors to specific tRNAs, small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs) are all enriched in virions. Consistent with their cytoplasmic recruitment, packaging of both pre-tRNAs and U6 snRNA requires the nuclear export receptor Exportin-5. Adenylated and uridylated forms of these RNAs accumulate in cells and virions when the cytoplasmic exoribonuclease DIS3L2 and subunits of the RNA exosome are depleted. Together, our data reveal that MLV recruits RNAs froma novel host cell surveillance pathway in which unprocessed and unneeded nuclear ncRNAs are exported to the cytoplasm for degradation. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Eckwahl%2C+Matthew+J%2E%22">Eckwahl, Matthew J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Soyeong+Sim%22">Soyeong Sim</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Smith%2C+Derek%22">Smith, Derek</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Telesnitsky%2C+Alice%22">Telesnitsky, Alice</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Wolin%2C+Sandra+L%2E%22">Wolin, Sandra L.</searchLink><relatesTo>1,2,4</relatesTo><i> sandra.wolin@yale.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Genes+%26+Development%22">Genes & Development</searchLink>. 2015, Vol. 29 Issue 6, p646-657. 12p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22MOLONEY+murine+leukemia+virus%22">MOLONEY murine leukemia virus</searchLink><br />*<searchLink fieldCode="DE" term="%22RETROVIRUSES%22">RETROVIRUSES</searchLink><br />*<searchLink fieldCode="DE" term="%22LEUKEMIA%22">LEUKEMIA</searchLink><br />*<searchLink fieldCode="DE" term="%22SMALL+nuclear+RNA%22">SMALL nuclear RNA</searchLink><br />*<searchLink fieldCode="DE" term="%22LABORATORY+mice%22">LABORATORY mice</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Although all retroviruses recruit host cell RNAs into virions, both the spectrum of RNAs encapsidated and the mechanisms by which they are recruited remain largely unknown. Here, we used high-throughput sequencing to obtain a comprehensive description of the RNAs packaged by a model retrovirus, murine leukemia virus. The major encapsidated host RNAs are noncoding RNAs (ncRNAs) and members of the VL30 class of endogenous retroviruses. Remarkably, although Moloney leukemia virus (MLV) assembles in the cytoplasm, precursors to specific tRNAs, small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs) are all enriched in virions. Consistent with their cytoplasmic recruitment, packaging of both pre-tRNAs and U6 snRNA requires the nuclear export receptor Exportin-5. Adenylated and uridylated forms of these RNAs accumulate in cells and virions when the cytoplasmic exoribonuclease DIS3L2 and subunits of the RNA exosome are depleted. Together, our data reveal that MLV recruits RNAs froma novel host cell surveillance pathway in which unprocessed and unneeded nuclear ncRNAs are exported to the cytoplasm for degradation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Genes & Development is the property of Cold Spring Harbor Laboratory Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1101/gad.258731.115 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 646 Subjects: – SubjectFull: MOLONEY murine leukemia virus Type: general – SubjectFull: RETROVIRUSES Type: general – SubjectFull: LEUKEMIA Type: general – SubjectFull: SMALL nuclear RNA Type: general – SubjectFull: LABORATORY mice Type: general Titles: – TitleFull: A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Eckwahl, Matthew J. – PersonEntity: Name: NameFull: Soyeong Sim – PersonEntity: Name: NameFull: Smith, Derek – PersonEntity: Name: NameFull: Telesnitsky, Alice – PersonEntity: Name: NameFull: Wolin, Sandra L. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 03 Text: 2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 08909369 Numbering: – Type: volume Value: 29 – Type: issue Value: 6 Titles: – TitleFull: Genes & Development Type: main |
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