A Small-Volume, Low-Cost, and Versatile Continuous Culture Device.
| Title: | A Small-Volume, Low-Cost, and Versatile Continuous Culture Device. |
|---|---|
| Authors: | Matteau, Dominick1, Baby, Vincent1, Pelletier, Stéphane2, Rodrigue, Sébastien1 sebastien.rodrigue@usherbrooke.ca |
| Source: | PLoS ONE. 7/21/2015, Vol. 10 Issue 7, p1-13. 13p. |
| Subject Terms: | *CONTINUOUS culture (Microbiology), *COST analysis, *CELL growth, *CHEMOSTAT, *CELL populations, *LIGHT emitting diodes |
| Abstract: | Background: Continuous culture devices can be used for various purposes such as establishing reproducible growth conditions or maintaining cell populations under a constant environment for long periods. However, commercially available instruments are expensive, were not designed to handle small volumes in the milliliter range, and can lack the flexibility required for the diverse experimental needs found in several laboratories. Methodology/Principal Findings: We developed a versatile continuous culture system and provide detailed instructions as well as a graphical user interface software for potential users to assemble and operate their own instrument. Three culture chambers can be controlled simultaneously with the proposed configuration, and all components are readily available from various sources. We demonstrate that our continuous culture device can be used under different modes, and can easily be programmed to behave either as a turbidostat or chemostat. Addition of fresh medium to the culture vessel can be controlled by a real-time feedback loop or simply calibrated to deliver a defined volume. Furthermore, the selected light-emitting diode and photodetector enable the use of phenol red as a pH indicator, which can be used to indirectly monitor the bulk metabolic activity of a cell population rather than the turbidity. Conclusions/Significance: This affordable and customizable system will constitute a useful tool in many areas of biology such as microbial ecology as well as systems and synthetic biology. [ABSTRACT FROM AUTHOR] |
| Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Academic Search Complete |
| FullText | Links: – Type: pdflink Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:a9h&genre=article&issn=19326203&ISBN=&volume=10&issue=7&date=20150721&spage=1&pages=1-13&title=PLoS ONE&atitle=A%20Small-Volume%2C%20Low-Cost%2C%20and%20Versatile%20Continuous%20Culture%20Device.&aulast=Matteau%2C%20Dominick&id=DOI:10.1371/journal.pone.0133384 Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: a9h DbLabel: Academic Search Complete An: 108734125 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: A Small-Volume, Low-Cost, and Versatile Continuous Culture Device. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Matteau%2C+Dominick%22">Matteau, Dominick</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Baby%2C+Vincent%22">Baby, Vincent</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pelletier%2C+Stéphane%22">Pelletier, Stéphane</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodrigue%2C+Sébastien%22">Rodrigue, Sébastien</searchLink><relatesTo>1</relatesTo><i> sebastien.rodrigue@usherbrooke.ca</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22PLoS+ONE%22">PLoS ONE</searchLink>. 7/21/2015, Vol. 10 Issue 7, p1-13. 13p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22CONTINUOUS+culture+%28Microbiology%29%22">CONTINUOUS culture (Microbiology)</searchLink><br />*<searchLink fieldCode="DE" term="%22COST+analysis%22">COST analysis</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+growth%22">CELL growth</searchLink><br />*<searchLink fieldCode="DE" term="%22CHEMOSTAT%22">CHEMOSTAT</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+populations%22">CELL populations</searchLink><br />*<searchLink fieldCode="DE" term="%22LIGHT+emitting+diodes%22">LIGHT emitting diodes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background: Continuous culture devices can be used for various purposes such as establishing reproducible growth conditions or maintaining cell populations under a constant environment for long periods. However, commercially available instruments are expensive, were not designed to handle small volumes in the milliliter range, and can lack the flexibility required for the diverse experimental needs found in several laboratories. Methodology/Principal Findings: We developed a versatile continuous culture system and provide detailed instructions as well as a graphical user interface software for potential users to assemble and operate their own instrument. Three culture chambers can be controlled simultaneously with the proposed configuration, and all components are readily available from various sources. We demonstrate that our continuous culture device can be used under different modes, and can easily be programmed to behave either as a turbidostat or chemostat. Addition of fresh medium to the culture vessel can be controlled by a real-time feedback loop or simply calibrated to deliver a defined volume. Furthermore, the selected light-emitting diode and photodetector enable the use of phenol red as a pH indicator, which can be used to indirectly monitor the bulk metabolic activity of a cell population rather than the turbidity. Conclusions/Significance: This affordable and customizable system will constitute a useful tool in many areas of biology such as microbial ecology as well as systems and synthetic biology. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=a9h&AN=108734125 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1371/journal.pone.0133384 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: CONTINUOUS culture (Microbiology) Type: general – SubjectFull: COST analysis Type: general – SubjectFull: CELL growth Type: general – SubjectFull: CHEMOSTAT Type: general – SubjectFull: CELL populations Type: general – SubjectFull: LIGHT emitting diodes Type: general Titles: – TitleFull: A Small-Volume, Low-Cost, and Versatile Continuous Culture Device. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Matteau, Dominick – PersonEntity: Name: NameFull: Baby, Vincent – PersonEntity: Name: NameFull: Pelletier, Stéphane – PersonEntity: Name: NameFull: Rodrigue, Sébastien IsPartOfRelationships: – BibEntity: Dates: – D: 21 M: 07 Text: 7/21/2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 19326203 Numbering: – Type: volume Value: 10 – Type: issue Value: 7 Titles: – TitleFull: PLoS ONE Type: main |
| ResultId | 1 |