In vitro Propagation and Acclimatization of Achillea fragrantissima Frossk Sch. Bip.
| Title: | In vitro Propagation and Acclimatization of Achillea fragrantissima Frossk Sch. Bip. |
|---|---|
| Authors: | Shibli, Rida A.1, Al-Qudah, Tamara S.2, Younes, Laila Sasi3 |
| Source: | Jordan Journal of Agricultural Sciences. 2015, Vol. 11 Issue 2, p339-351. 13p. |
| Subject Terms: | *YARROW, *PLANT micropropagation, *ACCLIMATIZATION (Plants), *MEDICINAL plants, *PLANT tissue culture |
| Abstract: | A. fragrantissima is one of the most Achillia species found in Jordan, it is known by local communities as Qaisoum or Qisum. Most effective method found to increase and conserve herbal plant is in vitro propagation. It is considered to be easier and more rapid for herbaceous plants than woody species. In the current study, in vitro propagation of A. fragrantissima rooting and acclimatization was studied. Complete germination of seeds (100%) was obtained in vitro on water-agar medium and developed into plantlets with greater hypocotyl and root length, and full cotyledonary leaves. Multiplication of mother stock was established on MS (Murashige and Skoog) media supplemented with 1mg/L GA3 after 4 weeks of culture. Proliferation was experimented with different levels of cytokinin (0.0, 0.4, 0.6, 0.8, 1.2 or 1.6 mg/L) of BA, kinetin or 2ip. Maximum proliferation of Achillea fragrantissima (8 shoot/explant) was obtained when MS medium supplemented with 1.2 mg/L kinetin. Rooting was experimented at different levels of auxins (0.0, 0.4, 0.6, 0.8, 1.2, or 1.6 mg/L) of NAA, IAA or IBA. Highest root number (9.80) and length (1.80 cm) was obtained at 0.4 mg/L NAA, while IAA failed to promote root induction. Rooted plantlets were successfully acclimatized with 100% survival. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: In vitro Propagation and Acclimatization of Achillea fragrantissima Frossk Sch. Bip. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Shibli%2C+Rida+A%2E%22">Shibli, Rida A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Al-Qudah%2C+Tamara+S%2E%22">Al-Qudah, Tamara S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Younes%2C+Laila+Sasi%22">Younes, Laila Sasi</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Jordan+Journal+of+Agricultural+Sciences%22">Jordan Journal of Agricultural Sciences</searchLink>. 2015, Vol. 11 Issue 2, p339-351. 13p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22YARROW%22">YARROW</searchLink><br />*<searchLink fieldCode="DE" term="%22PLANT+micropropagation%22">PLANT micropropagation</searchLink><br />*<searchLink fieldCode="DE" term="%22ACCLIMATIZATION+%28Plants%29%22">ACCLIMATIZATION (Plants)</searchLink><br />*<searchLink fieldCode="DE" term="%22MEDICINAL+plants%22">MEDICINAL plants</searchLink><br />*<searchLink fieldCode="DE" term="%22PLANT+tissue+culture%22">PLANT tissue culture</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A. fragrantissima is one of the most Achillia species found in Jordan, it is known by local communities as Qaisoum or Qisum. Most effective method found to increase and conserve herbal plant is in vitro propagation. It is considered to be easier and more rapid for herbaceous plants than woody species. In the current study, in vitro propagation of A. fragrantissima rooting and acclimatization was studied. Complete germination of seeds (100%) was obtained in vitro on water-agar medium and developed into plantlets with greater hypocotyl and root length, and full cotyledonary leaves. Multiplication of mother stock was established on MS (Murashige and Skoog) media supplemented with 1mg/L GA3 after 4 weeks of culture. Proliferation was experimented with different levels of cytokinin (0.0, 0.4, 0.6, 0.8, 1.2 or 1.6 mg/L) of BA, kinetin or 2ip. Maximum proliferation of Achillea fragrantissima (8 shoot/explant) was obtained when MS medium supplemented with 1.2 mg/L kinetin. Rooting was experimented at different levels of auxins (0.0, 0.4, 0.6, 0.8, 1.2, or 1.6 mg/L) of NAA, IAA or IBA. Highest root number (9.80) and length (1.80 cm) was obtained at 0.4 mg/L NAA, while IAA failed to promote root induction. Rooted plantlets were successfully acclimatized with 100% survival. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Jordan Journal of Agricultural Sciences is the property of University of Jordan and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.12816/0030430 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 339 Subjects: – SubjectFull: YARROW Type: general – SubjectFull: PLANT micropropagation Type: general – SubjectFull: ACCLIMATIZATION (Plants) Type: general – SubjectFull: MEDICINAL plants Type: general – SubjectFull: PLANT tissue culture Type: general Titles: – TitleFull: In vitro Propagation and Acclimatization of Achillea fragrantissima Frossk Sch. Bip. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shibli, Rida A. – PersonEntity: Name: NameFull: Al-Qudah, Tamara S. – PersonEntity: Name: NameFull: Younes, Laila Sasi IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: 2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 18158625 Numbering: – Type: volume Value: 11 – Type: issue Value: 2 Titles: – TitleFull: Jordan Journal of Agricultural Sciences Type: main |
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