Over-the-counter carrageenan-based sprays may interfere with PCR testing of nasopharyngeal swabs to detect SARS-CoV-2.

Authors: Corocher, Taylor^1,2 (AUTHOR) taylor.corocher@nh.org.au, Edwards, Kira^1,2 (AUTHOR), Hersusianto, Yvonne^1,3 (AUTHOR), Campbell, Donald^4,5 (AUTHOR), Lim, Hui Yin^1,2,6,7,8 (AUTHOR), Monagle, Paul^9,10,11,12 (AUTHOR), Ho, Prahlad^1,2,6,7,8 (AUTHOR)

Source: PLoS ONE. 2/6/2025, Vol. 20 Issue 2, p1-12. 12p.

Subject Terms: *DIAGNOSTIC use of polymerase chain reaction, *POLYMERASE chain reaction, *INTRANASAL medication, *INFECTIOUS disease transmission, *RESPIRATORY diseases, *CARRAGEENANS, *HEPARIN

Comparative Analysis of Viral Load Quantification Using Reverse Transcription Polymerase Chain Reaction and Digital Droplet Polymerase Chain Reaction.

Authors: Gebe Abreu Cabral, Paula¹ (AUTHOR) pgacabral99@gmail.com, Bastos de Souza, Sávio¹ (AUTHOR) saviobas@gmail.com, Ferraz Arruda, Raul¹ (AUTHOR) raul_arruda@hotmail.com, Passos de Figueiredo Cabral, Sheila¹ (AUTHOR) sheila.figueiredo@icloud.com, Leone Evangelista Monteiro de Assis, Arícia¹ (AUTHOR) aricialeone@hotmail.com, Porto Muniz Martins, Yolanda¹ (AUTHOR) yolymartins@icloud.com, Brazil Viana Junior, Antônio² (AUTHOR) brazil.estatistico@gmail.com, Chang, Junbiao³ (AUTHOR) changjunbiao@zzu.edu.cn, Lei, Pingsheng⁴ (AUTHOR) lei@imm.ac.cn, Martins da Silva, Renato¹ (AUTHOR) renatomartins@galzu.org.br

Source: International Journal of Molecular Sciences. Jan2025, Vol. 26 Issue 2, p725. 9p.

Subject Terms: *SARS-CoV-2, *REVERSE transcriptase polymerase chain reaction, *COVID-19, *POLYMERASE chain reaction, *VIRAL load

Dissecting the major genetic components underlying cotton lint development.

Authors: Sun, Yali^1,2, Yuan, Yuman³, He, Shoupu¹, Stiller, Warwick⁴, Wilson, Iain³, Du, Xiongming¹, duxiongming@caas.cn, Zhu, Qian-Hao³, qianhao.zhu@csiro.au

Source: Genetics; Feb2024, Vol. 226 Issue 2, p1-13, 13p

Real-Time Polymerase Chain Reaction Systems for Detection and Differentiation of Unclassified Viruses of the Phenuiviridae Family.

Authors: Dereventsova, Alena V.¹, alena.dereventsova@gmail.com, Klimentov, Alexander S.^1,2, Kholodilov, Ivan S.^2,3, Belova, Oxana A.^2,4, Butenko, Alexander M.³, Karganova, Galina G.^2,4

Source: Methods & Protocols; Feb2025, Vol. 8 Issue 1, p20, 15p

ROLE OF OUTER MEMBRANE VESICLES OF HYPERVIRULENT KLEBSIELLA PNEUMONIAE ISOLATED FROM BOVINE UTERINE DISCHARGE IN PRO-INFLAMMATORY DYSREGULATION.

Authors: JABER, A. H.¹, NEAMAH, A.², AL-KARAGOLY, H.³ hassan.aliwee@qu.edu.iq

Source: Bulgarian Journal of Veterinary Medicine. Mar2025, Vol. 28 Issue 1, p117-132. 16p.

Subject Terms: *EXTRACELLULAR vesicles, *DIAGNOSTIC use of polymerase chain reaction, *GRAM-negative bacteria, *ULTRACENTRIFUGATION, *MOLECULAR weights

Real-time PCR and immunohistochemistry detection of Wolbachia in adult Dirofilaria immitis from dogs treated with doxycycline and ivermectin.

Authors: Chu, Yi¹ (AUTHOR) yc611@georgetown.edu, Sakamoto, Kaori² (AUTHOR), Evans, Christopher C.³ (AUTHOR), Dzimianski, Michael T.³ (AUTHOR), Fricks, Crystal⁴ (AUTHOR), Mansour, Abdelmoneim⁴ (AUTHOR), DiCosty, Utami⁴ (AUTHOR), McCall, Scott⁴ (AUTHOR), McCall, John W.⁴ (AUTHOR), Nelson, C. Thomas⁵ (AUTHOR), Moorhead, Andrew R.^2,6 (AUTHOR) armoorh2@ncsu.edu

Source: Parasites & Vectors. 2/26/2025, Vol. 18 Issue 1, p1-10. 10p.

Subject Terms: *DIROFILARIA immitis, *POLYMERASE chain reaction, *WOLBACHIA, *MULTIPLE comparisons (Statistics), *REST periods

Diversity and natural infection of phlebotomine sand flies (Diptera, Psychodidae) in an endemic area of American tegumentary leishmaniasis in southeastern Bahia, Brazil.

Authors: Cova, Bruno Oliveira^1,2,3 (AUTHOR) bruno_cova@yahoo.com.br, de Oliveira, Livia Alves^1,2 (AUTHOR) livia.aoliveira@ebserh.gov.br, Lima Machado, Paulo Roberto^1,2 (AUTHOR) prlmachado@hotmail.com, de Carvalho, Edgar Marcelino^2,4 (AUTHOR) imuno@ufba.br, Monte-Alegre, Adriano Figueiredo^2,5 (AUTHOR) adriano.monte@ufba.br, Schriefer, Albert^1,2,5 (AUTHOR) nab.schriefer@gmail.com

Source: Parasites & Vectors. 2/26/2025, Vol. 18 Issue 1, p1-10. 10p.

Subject Terms: *PSYCHODIDAE, *POLYMERASE chain reaction, *LEISHMANIASIS, *DIPTERA, *CACAO, *SAND flies

Geographic Terms: BAHIA (Brazil : State), BRAZIL

Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

Authors: Naderzadeh, Elham¹ (AUTHOR) sweets_e84@yahoo.com, Kargar, Mohammad² (AUTHOR) mkargar@jia.ac.ir, Mokhtari, Mohammad Javad³ (AUTHOR) mj.mokhtari@iau.ac.ir, Farhadi, Ali^4,5 (AUTHOR) farhadi_a@sums.ac.ir

Source: Virology Journal. 2/24/2025, Vol. 22 Issue 1, p1-11. 11p.

Subject Terms: *REVERSE transcriptase polymerase chain reaction, *TRANSCRIPTION factors, *GENE expression, *MEDICAL sciences, *HUMAN papillomavirus, *DNA damage, *GENETIC toxicology

New insights into the range and transmission dynamics of a koala gammaherpesvirus, phascolarctid gammaherpesvirus 2.

Authors: Church, Chloe¹ (AUTHOR), Casteriano, Andrea¹ (AUTHOR), Muir, Yasmine SS.¹ (AUTHOR), Krockenberger, Mark¹ (AUTHOR), Vaz, Paola K.² (AUTHOR), Higgins, Damien P.¹ (AUTHOR), Wright, Belinda R.¹ (AUTHOR) belinda.wright@sydney.edu.au

Source: Scientific Reports. 2/20/2025, Vol. 15 Issue 1, p1-8. 8p.

Subject Terms: *PUBLIC health surveillance, *POLYMERASE chain reaction, *INFECTIOUS disease transmission, *KOALA, *LIFE sciences

N6-methyladenosine (m6A)-forming enzyme METTL3 controls UAF1 stability to promote inflammation in a model of colitis by stimulating NLRP3.

Authors: Lai, Yongqiang^1,2 (AUTHOR) lyqfrank@163.com, Liu, Junhao² (AUTHOR), Hu, Xiao² (AUTHOR), Zeng, Xiancheng² (AUTHOR), Gao, Peng² (AUTHOR)

Source: Scientific Reports. 2/18/2025, Vol. 15 Issue 1, p1-14. 14p.

Subject Terms: *REVERSE transcriptase polymerase chain reaction, *NLRP3 protein, *ULCERATIVE colitis, *WEIGHT loss, *DEXTRAN sulfate

Evaluating Rabies Test Accuracy: A Systematic Review and Meta-Analysis of Human and Canine Diagnostic Methods.

Authors: Candia-Puma, Mayron Antonio^1,2 (AUTHOR) mcandia@ucsm.edu.pe, Pola-Romero, Leydi¹ (AUTHOR) hbarazorda@ucsm.edu.pe, Barazorda-Ccahuana, Haruna Luz¹ (AUTHOR) lgoyzueta@ucsm.edu.pe, Goyzueta-Mamani, Luis Daniel¹ (AUTHOR), Galdino, Alexsandro Sobreira^3,4 (AUTHOR) asgaldino@ufsj.edu.br, Machado-de-Ávila, Ricardo Andrez⁵ (AUTHOR) r_andrez@unesc.net, Giunchetti, Rodolfo Cordeiro^6,7 (AUTHOR) giunchetti@icb.ufmg.br, Ferraz Coelho, Eduardo Antonio⁸ (AUTHOR) eduardoferrazcoelho@yahoo.com.br, Chávez-Fumagalli, Miguel Angel¹ (AUTHOR) mchavezf@ucsm.edu.pe

Source: Diagnostics (2075-4418). Feb2025, Vol. 15 Issue 4, p412. 27p.

Subject Terms: *REVERSE transcriptase polymerase chain reaction, *ENZYME-linked immunosorbent assay, *ANTIBODY titer, *RABIES, DEVELOPING countries

Detection of Leishmania spp. in Small Non-Flying Mammals (Didelphimorphia and Rodentia) from Bahia, Northeast Brazil.

Authors: de Souza, Graziela Baroni¹ (AUTHOR) grazielabaroni@hotmail.com, Fehlberg, Hllytchaikra Ferraz¹ (AUTHOR) ferrazhellen@hotmail.com, Rosa, Beatris Felipe² (AUTHOR) beatris.rosa@hotmail.com, Ribeiro, Cássia Matos³ (AUTHOR) cassia.matos@hotmail.com, Sevá, Anaiá da Paixão⁴ (AUTHOR), Maciel, Bianca Mendes⁵ (AUTHOR), Alvarez, Martin Roberto Del Valle⁵ (AUTHOR), Albuquerque, George Rêgo⁴ (AUTHOR), Silva, Fabiana Lessa⁴ (AUTHOR) flsilva@uesc.br

Source: Animals (2076-2615). Feb2025, Vol. 15 Issue 4, p588. 12p.

Subject Terms: *POLYMERASE chain reaction, *DOMESTIC animals, *LEISHMANIASIS, *MOLECULAR diagnosis, *RODENTS

Evaluating the impact of avian paramyxovirus type 1 infection in poultry at live bird markets in Nigeria: defining hurdles to sustainable agriculture.

Authors: Ekiri, Abel B.¹ (AUTHOR) ab.ekiri@surrey.ac.uk, Wakawa, Aliyu² (AUTHOR), Reid, Scott M.³ (AUTHOR), James, Joe^3,4 (AUTHOR), Ross, Craig³ (AUTHOR), Byrne, Alexander M. P.³ (AUTHOR), Lewis, Thomas^3,4 (AUTHOR), Lynton-Jenkins, Joshua^3,4 (AUTHOR), Adebowale, Kehinde⁵ (AUTHOR), Mijten, Erik⁵ (AUTHOR), Varga, Gabriel⁵ (AUTHOR), Brown, Ian H.^3,4 (AUTHOR), Banyard, Ashley C.^3,4 (AUTHOR), Cook, Alasdair J. C.¹ (AUTHOR)

Source: BMC Veterinary Research. 2/12/2025, Vol. 21 Issue 1, p1-15. 15p.

Subject Terms: *REVERSE transcriptase polymerase chain reaction, *NEWCASTLE disease, *NEWCASTLE disease virus, *WHOLE genome sequencing, *ENZYME-linked immunosorbent assay

Geographic Terms: NIGERIA, AFRICA

Antibodies response in symptomatic and asymptomatic SARS-CoV-2 infected persons in Thailand.

Authors: Ruchisrisarod, Chanida¹ (AUTHOR) jeabchanida@gmail.com, Wanthong, Phanni¹ (AUTHOR), Joyjinda, Yutthana¹ (AUTHOR), Bunprakob, Saowalak¹ (AUTHOR), Hemachudha, Pasin¹ (AUTHOR), Mungaomklang, Anek² (AUTHOR), Supharatpariyakorn, Thirawat¹ (AUTHOR), Hemachudha, Thiravat¹ (AUTHOR), Wasontiwong, Abhinbhen Saraya¹ (AUTHOR)

Source: PLoS ONE. 2/11/2025, Vol. 20 Issue 2, p1-11. 11p.

Subject Terms: *SARS-CoV-2, *REVERSE transcriptase polymerase chain reaction, *COVID-19, *VIRAL antibodies, *ANTIBODY formation

Pre-departure PCR testing of travellers for SARS-CoV-2 was an effective tool in limiting transmission in Greenland during the early phases of the COVID-19 pandemic.

Authors: Møller, Mie^1,2,3,4 (AUTHOR) mimoe@uni.gl, Moestrup, Kasper Sommerlund⁵ (AUTHOR), Abelsen, Trine⁶ (AUTHOR), Vedsted, Peter^7,8 (AUTHOR), Mølbak, Kåre^2,3 (AUTHOR), Koch, Anders^1,3,4,9 (AUTHOR)

Source: BMC Public Health. 2/13/2025, Vol. 25 Issue 1, p1-9. 9p.

Subject Terms: *COVID-19 testing, *DIAGNOSTIC use of polymerase chain reaction, *MEDICAL screening, *COVID-19, *INFECTIOUS disease transmission

Geographic Terms: GREENLAND

Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice.

Authors: Kasper, Maren¹ (AUTHOR) maren.kasper@uveitis-zentrum.de, Karlstetter, Marcus² (AUTHOR), Wildschütz, Lena¹ (AUTHOR), Scholz, Rebecca² (AUTHOR), Busch, Martin¹ (AUTHOR), Bauer, Dirk¹ (AUTHOR), Meyer zu Hörste, Gerd³ (AUTHOR), Thanos, Solon⁴ (AUTHOR), Langmann, Thomas² (AUTHOR), Heiligenhaus, Arnd^1,5 (AUTHOR)

Source: Journal of Neuroinflammation. 2/10/2025, Vol. 22 Issue 1, p1-11. 11p.

Subject Terms: *LIFE sciences, *POLYMERASE chain reaction, *GENE clusters, *CARRIER proteins, *MICROGLIA

CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis.

Authors: Wu, Jianqiang¹ (AUTHOR) wjqclx1993@163.com, Chen, Lixia² (AUTHOR), Wen, Peijun¹ (AUTHOR)

Source: Scientific Reports. 2/4/2025, Vol. 15 Issue 1, p1-18. 18p.

Subject Terms: *MEDICAL sciences, *POLYMERASE chain reaction, *MELANOMA, *CAUSES of death, *WESTERN immunoblotting

First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024).

Authors: Kiwan, Paloma¹ (AUTHOR) kiwan_p@univ-corse.fr, Lopez, Eva¹ (AUTHOR) lopez_e@univ-corse.fr, Gasparine, Morena¹ (AUTHOR) gasparine_m@univ-corse.fr, Piorkowski, Geraldine¹ (AUTHOR) geraldine.piorkowski@inserm.fr, Colmant, Agathe¹ (AUTHOR) agathe.COLMANT@univ-amu.fr, Paguem, Achille² (AUTHOR) achillepaguem@gmail.com, Mvodo, Stephanie² (AUTHOR) mvodostephanie@gmail.com, Thirion, Laurence¹ (AUTHOR) laurence.thirion@ird.fr, de Lamballerie, Xavier^1,3 (AUTHOR) Xavier.De-Lamballerie@univ-amu.fr, Charrel, Remi¹ (AUTHOR) remi.charrel@univ-amu.fr, Falchi, Alessandra¹ (AUTHOR) falchi_a@univ-corse.fr

Source: Parasites & Vectors. 2/5/2025, Vol. 18 Issue 1, p1-13. 13p.

Subject Terms: *REVERSE transcriptase polymerase chain reaction, *CATTLE tick, *RHIPICEPHALUS, *HYALOMMA, *CROSS-sectional method, *AMBLYOMMA

Geographic Terms: CAMEROON

A TaqMan-Based qRT-PCR Assay for Accurate Evaluation of the Oncogenic TrkAIII Splice Variant in Tumor cDNAs.

Authors: Sbaffone, Maddalena¹ (AUTHOR), Farina, Antonietta Rosella¹ (AUTHOR), Martelli, Ilaria¹ (AUTHOR) maddalena.sbaffone@graduate.univaq.it, Pontieri, Eugenio¹ (AUTHOR), Guadagni, Stefano¹ (AUTHOR), Mackay, Andrew Reay¹ (AUTHOR) andrewreay.mackay@univaq.it, Cappabianca, Lucia¹ (AUTHOR), Zelli, Veronica¹ (AUTHOR)

Source: Cancers. Feb2025, Vol. 17 Issue 3, p471. 16p.

Subject Terms: *RESEARCH funding, *REVERSE transcriptase polymerase chain reaction, *DNA, *DESCRIPTIVE statistics, *DNA probes, *RNA, *GENE expression, *TRANSFERASES, *SENSITIVITY & specificity (Statistics)

A study of the clinico-demographical profile of different dengue serotypes circulating in a Southern district of West Bengal, India.

Authors: Bose, Sayani¹, Laloo, Feradica D.², Maity, Partha Pratim³, Bhaduri, Anirban⁴ anir8365@gmail.com

Source: Asian Journal of Medical Sciences. Feb2025, Vol. 16 Issue 2, p69-73. 5p.

Subject Terms: *DENGUE hemorrhagic fever, *REVERSE transcriptase polymerase chain reaction, *DENGUE viruses, *VIRUS diseases, *DENGUE