Matrix metalloproteinases in dentin: Assessing their presence, activity, and inhibitors – a review of current trends

Authors: Anumula, Lavanya ^{a, ⁎}, Ramesh, Sindhu ^b, Kolaparthi, Venkata Suneel Kumar ^c

Source: In Dental Materials November 2024 40(11):2051-2073

Development of a sensitive high-throughput enzymatic assay capable of measuring sub-nanomolar inhibitors of SARS-CoV2 Mpro

Authors: Peter Kovar, Paul L Richardson, Alla Korepanova, Gustavo A Afanador, Vladimir Stojkovic, Tao Li, Michael R Schrimpf, Teresa I Ng, David A Degoey, Sujatha M Gopalakrishnan, Jun Chen

Source: SLAS Discovery, Vol 29, Iss 6, Pp 100179- (2024)

Subject Terms: Enzyme assays, Enzyme kinetics, SARS-CoV-2 main protease (Mpro), Positive cooperativity, Kosmotropic anion salts, Dimer-monomer equilibrium, Medicine (General), R5-920, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2472555224000418; https://doaj.org/toc/2472-5552

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The revealing of a novel double bond reductase related to perilla ketone biosynthesis in Perilla frutescens

Authors: Peina Zhou, Yongfang Shao, Zheng Jiang, Jingjie Dang, Cheng Qu, Qinan Wu

Source: BMC Plant Biology, Vol 23, Iss 1, Pp 1-12 (2023)

Subject Terms: Perilla frutescens, Transcriptome, Double bond reductase, Enzyme assays, Perilla ketone biosynthesis, Botany, QK1-989

File Description: electronic resource

Relation: https://doaj.org/toc/1471-2229

Access URL: https://doaj.org/article/91302a5760cc4d5e89ec84ec32bdb530

A Preclinical Study Comparing the Activity and Potency of OnabotulinumtoxinA and PrabotulinumtoxinA

Authors: Rupp DC, Canty D, Rhéaume C, Sondergaard B, Niño C, Broide RS, Brideau-Andersen AD

Source: Clinical, Cosmetic and Investigational Dermatology, Vol Volume 16, Pp 581-591 (2023)

Subject Terms: pharmaceutical preparations, biological assays, enzyme assays, animal studies, motor neurons, protein binding, Dermatology, RL1-803

File Description: electronic resource

Relation: https://www.dovepress.com/a-preclinical-study-comparing-the-activity-and-potency-of-onabotulinum-peer-reviewed-fulltext-article-CCID; https://doaj.org/toc/1178-7015

Access URL: https://doaj.org/article/0f48a9554c2b4ff1b192b61b2f491ef8

New gold pincer-type complexes induce caspase-dependent apoptosis in human cancer cells in vitro

Authors: Zarić Milan M., Čanović Petar P., Stanojević-Pirković Marijana, Knežević Sanja M., Živković-Zarić Radica S., Popovska-Joviĉić Biljana, Hamzagić Nedim, Simović-Marković Bojana, Marković Nenad, Rilak-Simović Ana

Source: Vojnosanitetski Pregled, Vol 78, Iss 8, Pp 865-873 (2021)

Subject Terms: antineoplastic agents, apoptosis, caspases, enzyme assays, gold, compounds, neoplasms, toxicity, tests, Medicine (General), R5-920

File Description: electronic resource

Relation: https://doaj.org/toc/0042-8450; https://doaj.org/toc/2406-0720

Access URL: https://doaj.org/article/564fe2a688e441debcc9f142c0a08ac6

A review of phosphatidate phosphatase assays

Authors: Prabuddha Dey, Gil-Soo Han, George M. Carman

Source: Journal of Lipid Research, Vol 61, Iss 12, Pp 1556-1564 (2020)

Subject Terms: Pah1, lipin, enzyme assays, radioactive assays, nonradioactive assays, diacylglycerol, Biochemistry, QD415-436

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S0022227520600189; https://doaj.org/toc/0022-2275

Access URL: https://doaj.org/article/21fab71ec1164ab094efc00f42dc7172

Undiagnosed Hepatitis B and C Virus Infection at a Teaching Hospital in Rawalpindi

Authors: Muhammad Imran Khan, Fadia Kalsoom, Fakhra Batool, Ahsan Kazmi, Qurat-ul-ain Zahra, Muhammad Bilal, Rizwan Ali, Ajkia Zaman Juthi, Hafiz M.N. Iqbal

Source: Journal of Pure and Applied Microbiology, Vol 14, Iss 2, Pp 1279-1286 (2020)

Subject Terms: hepatitis b, hepatitis c, public policy, enzyme assays, prevalence, Microbiology, QR1-502

File Description: electronic resource

Relation: https://microbiologyjournal.org/undiagnosed-hepatitis-b-and-c-virus-infection-at-a-teaching-hospital-in-rawalpindi/; https://doaj.org/toc/0973-7510; https://doaj.org/toc/2581-690X

Access URL: https://doaj.org/article/a2db6179555346c7a49969e47cc0d3b0

Serine 363 of a hydrophobic region of Archaeal ribulose 1,5-bisphosphate carboxylase/oxygenase from Archaeoglobus fulgidus and Thermococcus kodakaraensis affects CO2/O2 substrate specificity and oxygen sensitivity

Contributors: Berg, Ivan [Univ. of Freiburg (Germany)]

Source: PLoS ONE; 10; 9

File Description: Medium: ED; Size: Article No. e0138351

Access URL: http://www.osti.gov/scitech/servlets/purl/1324973

Structure activity relationship studies on Amb639752: toward the identification of a common pharmacophoric structure for DGKα inhibitors

Authors: Suresh Velnati, Alberto Massarotti, Annamaria Antona, Maria Talmon, Luigia Grazia Fresu, Alessandra Silvia Galetto, Daniela Capello, Alessandra Bertoni, Valentina Mercalli, Andrea Graziani, Gian Cesare Tron, Gianluca Baldanzi

Source: Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 35, Iss 1, Pp 96-108 (2020)

Subject Terms: diacylglycerol kinase, kinase inhibitors, structure–activity relationship, enzyme assays, molecular modelling, Therapeutics. Pharmacology, RM1-950

File Description: electronic resource

Relation: https://doaj.org/toc/1475-6366; https://doaj.org/toc/1475-6374

Access URL: https://doaj.org/article/3339702823c34737b631d4896dd549e8

Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Authors: Julian Geiger, Rebecca Doelker, Sofia Salö, Thomas Roitsch, Louise T. Dalgaard

Source: BMC Research Notes, Vol 12, Iss 1, Pp 1-6 (2019)

Subject Terms: INS-1E, Enzyme assays, 96 well format, Aldolase, Hexokinase, Glucose-6-phosphate dehydrogenase, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390

File Description: electronic resource

Relation: http://link.springer.com/article/10.1186/s13104-019-4697-y; https://doaj.org/toc/1756-0500

Access URL: https://doaj.org/article/77f8fee959de4f5f849e4f460251c342

DRfit: a Java tool for the analysis of discrete data from multi-well plate assays

Authors: Andreas Hofmann, Sarah Preston, Megan Cross, H. M. P. Dilrukshi Herath, Anne Simon, Robin B. Gasser

Source: BMC Bioinformatics, Vol 20, Iss 1, Pp 1-6 (2019)

Subject Terms: Biochemical assays, Data analysis, Dose-response experiments, Drug discovery, Enzyme assays, Computer applications to medicine. Medical informatics, R858-859.7, Biology (General), QH301-705.5

File Description: electronic resource

Relation: http://link.springer.com/article/10.1186/s12859-019-2891-5; https://doaj.org/toc/1471-2105

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Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

Authors: Nabiha Naeem Sheikhs, Qurat-ul-ain, Saba Altaf

Source: Bioscientific Review, Vol 2, Iss 4 (2020)

Subject Terms: bacterial co-culture, enzyme assays, hydrolytic enzymes, proteases, solid-state fermentation, Microbiology, QR1-502, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://journals.umt.edu.pk/index.php/BSR/article/view/726; https://doaj.org/toc/2663-4198; https://doaj.org/toc/2663-4201

Access URL: https://doaj.org/article/d39c2a6e499a4b60b54dd3762f4758f6

Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays

Authors: Andreas Hennig, Werner M. Nau

Source: Frontiers in Chemistry, Vol 8 (2020)

Subject Terms: cucurbiturils, enzyme assays, proteases, time-resolved fluorescence (TRF), peptides, Chemistry, QD1-999

File Description: electronic resource

Relation: https://www.frontiersin.org/article/10.3389/fchem.2020.00806/full; https://doaj.org/toc/2296-2646

Access URL: https://doaj.org/article/3c112f7593ec46b7862504c058266bde

Monitoring Protein Denaturation of Egg White Using Passive Microwave Radiometry (MWR)

Authors: Igor Goryanin, Lev Ovchinnikov, Sergey Vesnin, Yuri Ivanov

Source: Diagnostics, Vol 12, Iss 6, p 1498 (2022)

Subject Terms: microwave radiometry, passive microwave emission, protein denaturation, enzyme assays, drug discovery, brain trauma, Medicine (General), R5-920

File Description: electronic resource

Relation: https://www.mdpi.com/2075-4418/12/6/1498; https://doaj.org/toc/2075-4418

Access URL: https://doaj.org/article/242331a3ec5c4223ad87e2454fd1db89

Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS)

Authors: Kai Deng, Jijiao Zeng, Gang Cheng, Jian Gao, Kenneth L. Sale, Blake A. Simmons, Anup K. Singh, Paul D. Adams, Trent R. Northen

Source: Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-9 (2018)

Subject Terms: Lignin, β-Aryl ether, Lignin-modifying enzymes, NIMS, Enzyme assays, Fuel, TP315-360, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: http://link.springer.com/article/10.1186/s13068-018-1261-2; https://doaj.org/toc/1754-6834

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Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Authors: W. K. Dindi Chan, author¹, Marco Mason, author¹, Lee D. Hansen, author², Jason Donald Kenealey, author¹

Source: Journal of Visualized Experiments. (145)

Availability: http://www.jove.com/video/59302

Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

Authors: Alessio Peracchi, Eugenia Polverini

Source: Molecules, Vol 27, Iss 4, p 1398 (2022)

Subject Terms: substrate specificity, molecular discrimination, limits of specificity, enzyme assays, transaminases, enzyme evolution, Organic chemistry, QD241-441

File Description: electronic resource

Relation: https://www.mdpi.com/1420-3049/27/4/1398; https://doaj.org/toc/1420-3049

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Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges

Authors: Alonso Ornelas-González, Margarita Ortiz-Martínez, Mirna González-González, Marco Rito-Palomares

Source: Molecules, Vol 26, Iss 22, p 7026 (2021)

Subject Terms: enzyme assays, saliva, biomarkers, analysis, Organic chemistry, QD241-441

File Description: electronic resource

Relation: https://www.mdpi.com/1420-3049/26/22/7026; https://doaj.org/toc/1420-3049

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Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Authors: Mobeen Shaik, G. Girija Sankar, M. Iswarya, P. Rajitha

Source: Journal of Genetic Engineering and Biotechnology, Vol 15, Iss 1, Pp 87-94 (2017)

Subject Terms: Actinomycetes, Streptomyces, Bioactive metabolites, Phenotypic and genotypic characterization, Enzyme assays, Biotechnology, TP248.13-248.65, Genetics, QH426-470

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S1687157X17300124; https://doaj.org/toc/1687-157X

Access URL: https://doaj.org/article/10f2c9bfb3904ba0988f933acf24762e

Chapter Eleven - 4-Aldrithiol-based photometric assay for detection of methylthioalkylmalate synthase activity

Authors: Kitainda, Vivian, Jez, Joseph ^⁎

Source: In Methods in Enzymology 2024 702:229-245