QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Authors: Nelson, Glyn, Boehm, Ulrike, Bagley, Steve, Bajcsy, Peter, Bischof, Johanna, Brown, Claire M, Dauphin, Aurelien, Dobbie, Ian M, Eriksson, John E, Faklaris, Orestis, Fernandez-Rodriguez, Julia, Ferrand, Alexia, Gelman, Laurent, Gheisari, Ali, Hartmann, Hella, Kukat, Christian, Laude, Alex, Mitkovski, Miso, Munck, Sebastian, North, Alison J, Rasse, Tobias M, Resch-Genger, Ute, Schuetz, Lucas C, Seitz, Arne, Strambio-De-Castillia, Caterina, Swedlow, Jason R, Alexopoulos, Ioannis, Aumayr, Karin, Avilov, Sergiy, Bakker, Gert-Jan, Bammann, Rodrigo R, Bassi, Andrea, Beckert, Hannes, Beer, Sebastian, Belyaev, Yury, Bierwagen, Jakob, Birngruber, Konstantin A, Bosch, Manel, Breitlow, Juergen, Cameron, Lisa A, Chalfoun, Joe, Chambers, James J, Chen, Chieh-Li, Conde-Sousa, Eduardo, Corbett, Alexander D, Cordelieres, Fabrice P, Del Nery, Elaine, Dietzel, Ralf, Eismann, Frank, Fazeli, Elnaz, Felscher, Andreas, Fried, Hans, Gaudreault, Nathalie, Goh, Wah Ing, Guilbert, Thomas, Hadleigh, Roland, Hemmerich, Peter, Holst, Gerhard A, Itano, Michelle S, Jaffe, Claudia B, Jambor, Helena K, Jarvis, Stuart C, Keppler, Antje, Kirchenbuechler, David, Kirchner, Marcel, Kobayashi, Norio, Krens, Gabriel, Kunis, Susanne, Lacoste, Judith, Marcello, Marco, Martins, Gabriel G, Metcalf, Daniel J, Mitchell, Claire A, Moore, Joshua, Mueller, Tobias, Nelson, Michael S, Ogg, Stephen, Onami, Shuichi, Palmer, Alexandra L, Paul-Gilloteaux, Perrine, Pimentel, Jaime A, Plantard, Laure, Podder, Santosh, Rexhepaj, Elton, Royon, Arnaud, Saari, Markku A, Schapman, Damien, Schoonderwoert, Vincent, Schroth-Diez, Britta, Schwartz, Stanley, Shaw, Michael, Spitaler, Martin, Stoeckl, Martin T, Sudar, Damir, Teillon, Jeremie, Terjung, Stefan, Thuenauer, Roland, Wilms, Christian D, Wright, Graham D, Nitschke, Roland

Source: J. Microsc. 2021;1-18

Subject Terms: Quantitative Biology - Other Quantitative Biology, Physics - Instrumentation and Detectors

Access URL: http://arxiv.org/abs/2101.09153

Identification and quantification of senescent cell types by lamin B1 and HMGB1 in Actinic keratosis lesions

Authors: Wang, Audrey Shimei, Nakamizo, Satoshi, Ishida, Yoshihiro, Klassen, Genevieve, Chong, Priscilla, Wada, Aya, Lim, John S.Y., Wright, Graham D., Kabashima, Kenji, Dreesen, Oliver

Source: Journal of dermatological science. 105(1):61-64

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100154674233.0x000001&indx=1&recIds=ETOCvdc_100154674233.0x000001

Skin Whole‐Mount Immunofluorescent Staining Protocol, 3D Visualization, and Spatial Image Analysis

Authors: Schmidt, Alfonso J., Wright, Graham D., Ronchese, Franca, Price, Kylie M.

Source: Current protocols. 3(6)

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100186838928.0x000001&indx=1&recIds=ETOCvdc_100186838928.0x000001

A high throughput drug screening paradigm using transgenic Caenorhabditis elegans model of Alzheimer’s disease

Authors: Teo, Emelyne, Lim, Soon Yew John, Fong, Sheng, Larbi, Anis, Wright, Graham D., Tolwinski, Nicholas, Gruber, Jan

Source: In Translational Medicine of Aging 2020 4:11-21

Multiphoton Microscopy for Noninvasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalents

Authors: Sriram, Gopu, Sudhaharan, Thankiah, Wright, Graham D.

Source: Methods in molecular biology. 2150:195-212

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN624405698&indx=1&recIds=ETOCRN624405698

Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

Authors: Sridharan, Aishwarya, Lim, S. Y. John, Wright, Graham D., Vardy, Leah A.

Source: Methods in molecular biology. 2109:55-66

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN624193633&indx=1&recIds=ETOCRN624193633

A user-interactive algorithm quantifying nuclear pore complex distribution within the nuclear lamina network in single molecular localization microscopic image

Authors: Lim, John S.Y., Wright, Graham D., Burke, Brian, Xie, Wei

Source: In Methods 15 March 2019 157:42-46

Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells

Authors: Ong, Seow Theng, Wright, Graham D., Verma, Navin Kumar

Source: Methods in molecular biology. 1930:41-50

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN618868265&indx=1&recIds=ETOCRN618868265

Compartmentation of Mitochondrial and Oxidative Metabolism in Growing Hair Follicles: A Ring of Fire

Authors: Lemasters, John J., Ramshesh, Venkat K., Lovelace, Gregory L., Lim, John, Wright, Graham D., Harland, Duane, Dawson, Thomas L., Jr.

Source: In Journal of Investigative Dermatology July 2017 137(7):1434-1444

Recognising the importance and impact of Imaging Scientists: Global guidelines for establishing career paths within core facilities.

Authors: Wright, Graham D.¹ (AUTHOR), Thompson, Kerry A.² (AUTHOR), Reis, Yara³ (AUTHOR) yara.reis@embl.de, Bischof, Johanna⁴ (AUTHOR), Hockberger, Philip Edward⁵ (AUTHOR), Itano, Michelle S.⁶ (AUTHOR), Yen, Lisa⁷ (AUTHOR), Adelodun, Stephen Taiye⁸ (AUTHOR), Bialy, Nikki⁹ (AUTHOR), Brown, Claire M.¹⁰ (AUTHOR), Chaabane, Linda¹¹ (AUTHOR), Chew, Teng‐Leong¹² (AUTHOR), Chitty, Andrew I.¹³ (AUTHOR), Cordelières, Fabrice P.¹⁴ (AUTHOR), De Niz, Mariana¹⁵ (AUTHOR), Ellenberg, Jan¹⁶ (AUTHOR), Engelbrecht, Lize¹⁷ (AUTHOR), Fabian‐Morales, Eunice^18,19 (AUTHOR), Fazeli, Elnaz²⁰ (AUTHOR), Fernandez‐Rodriguez, Julia²¹ (AUTHOR)

Source: Journal of Microscopy. Jun2024, Vol. 294 Issue 3, p397-410. 14p.

Subject Terms: *GIFTED children, *CAREER development, *PROFESSIONAL employee training, *VOCATIONAL guidance, *EDUCATORS, *JOB security

Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

Authors: Conic, Sascha, Desplancq, Dominique, Ferrand, Alexia, Fischer, Veronique, Heyer, Vincent, San Martin, Bernardo Reina, Pontabry, Julien, Oulad-Abdelghani, Mustapha, Kishore Babu, N., Wright, Graham D.

Source: Journal of cell biology. 217(4):1537-1552

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN615468093&indx=1&recIds=ETOCRN615468093

Three-dimensional image analysis of the mouse cochlea

Authors: Wright, Graham D., Horn, Henning F.

Source: In Differentiation April-June 2016 91(4-5):104-108

Superresolution Microscopy of the Nuclear Envelope and Associated Proteins

Authors: Xie, Wei, Horn, Henning F., Wright, Graham D.

Source: Methods in molecular biology. 1411:83-98

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN606368055&indx=1&recIds=ETOCRN606368055

Tutorial: guidance for quantitative confocal microscopy

Authors: Jonkman, James, Brown, Claire M., Wright, Graham D., Anderson, Kurt I., North, Alison J.

Source: Nature Protocols; 20240101, Issue: Preprints p1-27, 27p

Superresolution microscopy reveals distinct localisation of full length IRSp53 and its I-BAR domain protein within filopodia

Authors: Sudhaharan, Thankiah, Hariharan, Srivats, Lim, John Soon Yew, Liu, Jaron Zhongliang, Koon, Yen Ling, Wright, Graham D., Chiam, Keng Hwee, Ahmed, Sohail

Source: Scientific reports. 9(1)

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100131268111.0x000001&indx=1&recIds=ETOCvdc_100131268111.0x000001

Mitochondrial Dysfunction in CD4+ T Effector Memory RA+ Cells.

Authors: Strickland, Marie, Lee, Salanne, Neo, Shi Yong, Balachander, Akhila, Low, Ivy, Mustafah, Seri, Goh, Wah Ing, Wright, Graham D., Larbi, Anis, Pender, Sylvia L. F.

Source: Biology (2079-7737); Apr2023, Vol. 12 Issue 4, p597, 14p

Subject Terms: IMMUNOSENESCENCE, CD4 antigen, T cells, OLDER people, GLUCOSE transporters, MITOCHONDRIA, IMMUNOLOGIC memory

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

Authors: Merigliano, Chiara¹, Burla, Romina^1,2, La Torre, Mattia¹, Del Giudice, Simona¹, Teo, Hsiangling³, Liew, Chong Wai³, Chojnowski, Alexandre^4,5, Goh, Wah Ing⁶, Olmos, Yolanda^7,8, Maccaroni, Klizia¹, Giubettini, Maria⁹, Chiolo, Irene¹⁰, Carlton, Jeremy G.^7,8, Raimondo, Domenico¹¹, Vernì, Fiammetta¹, Stewart, Colin L.^4,12, Rhodes, Daniela^3,13, Wright, Graham D.⁶, Burke, Brian E.⁵, Saggio, Isabella^1,2,3,13, Isabella.saggio@uniroma1.it

Source: PLoS Genetics; 8/27/2021, Vol. 17 Issue 8, p1-30, 30p

Experimentally manipulating fungi with optical tweezers*

Authors: Wright, Graham D., Read, Nick D., Arlt, Jochen, Poon, Wilson C.K.

Source: In Mycoscience January 2007 48(1):15-19

Myosin concentration underlies cell size--dependent scalability of actomyosin ring constriction.

Authors: Calvert, Meredith E. K., Wright, Graham D., Leong, Fong Yew

Source: Journal of Cell Biology; November 28 2011, Vol. 195 Issue 5, p13-1-13-15, 15p

Desmosome dualism -- most of the junction is stable, but a plakophilin moiety is persistently dynamic.

Authors: Fülle, Judith B., Huppert, Henri, Liebl, David, Jaron Liu, de Almeida, Rogerio Alves, Yanes, Bian, Wright, Graham D., Lane, E. Birgitte, Garrod, David R., Ballestrem, Christoph

Source: Journal of Cell Science; Nov2021, Vol. 134 Issue 21, p1-15, 15p

Subject Terms: CELL junctions, MOIETIES (Chemistry), DESMOSOMES, CYTOPLASMIC filaments, CELL membranes, CORAL bleaching