Combining steric exclusion with anion exchange – development of a universal and scalable adeno-associated virus downstream process

Authors: Meierrieks, Frederik ^{a, ⁎}, Rosario, Juan-Carlos ^b, Rübeling, Lena ^a, Asikoglu, Hatice ^a, Pflanz, Karl ^c, Pickl, Andreas ^a, Kusch, Kathrin ^{d, e}, Wolff, Michael W. ^{f, 1}, Graf, Benjamin ^{a, 1}

Source: In Journal of Chromatography A 22 February 2025 1743

Stability studies for the identification of critical process parameters for a pharmaceutical production of the Orf virus

Authors: Eilts, Friederike, Labisch, Jennifer J., Orbay, Sabri, Harsy, Yasmina M.J., Steger, Marleen, Pagallies, Felix, Amann, Ralf, Pflanz, Karl, Wolff, Michael W.

Source: Vaccine. 41(32):4731-4742

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A robust and efficient alluvial filtration method for the clarification of adeno-associated viruses from crude cell lysates

Authors: Meierrieks, Frederik, Pickl, Andreas, Wolff, Michael W.

Source: Journal of biotechnology. 367:31-41

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Purification and concentration of infectious koi herpesvirus using steric exclusion chromatography

Authors: Eilts, Friederike, Jordan, Lisa K., Harsy, Yasmina M. J., Bergmann, Sven M., Becker, Anna M., Wolff, Michael W.

Source: Journal of fish diseases. 46(8):873-886

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Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes

Authors: Meierrieks, Frederik, Kour, Ahmad, Pätz, Marvin, Pflanz, Karl, Wolff, Michael W., Pickl, Andreas

Source: In Molecular Therapy - Methods & Clinical Development 14 December 2023 31

Process intensification for the production of a C-tagged antimicrobial peptide in Escherichia coli – First steps toward a platform technology

Authors: Lappöhn, Carolin A., Oestreich, Arne M., Stei, Robin, Weber, Linus G., Maerz, Lea, Wolff, Michael W.

Source: In Journal of Bioscience and Bioengineering November 2023 136(5):358-365

An investigation of excipients for a stable Orf viral vector formulation

Authors: Eilts, Friederike, Harsy, Yasmina M.J., Lothert, Keven, Pagallies, Felix, Amann, Ralf, Wolff, Michael W.

Source: In Virus Research 15 October 2023 336

Optimization and validation of analytical affinity chromatography for the in-process monitoring and quantification of peptides containing a C-tag

Authors: Lappöhn, Carolin A., Maerz, Lea, Stei, Robin, Weber, Linus G., Wolff, Michael W.

Source: In Journal of Chromatography B 1 September 2023 1229

The diverse role of heparan sulfate and other GAGs in SARS-CoV-2 infections and therapeutics

Authors: Eilts, Friederike, Bauer, Sarah, Fraser, Keith, Dordick, Jonathan S., Wolff, Michael W., Linhardt, Robert J., Zhang, Fuming

Source: In Carbohydrate Polymers 1 January 2023 299

Comparison of sample preparation techniques for the physicochemical characterization of Orf virus particles

Authors: Eilts, Friederike, Steger, Marleen, Pagallies, Felix, Rziha, Hanns-Joachim, Hardt, Martin, Amann, Ralf, Wolff, Michael W.

Source: In Journal of Virological Methods December 2022 310

Quantification methods for viruses and virus-like particles applied in biopharmaceutical production processes

Authors: Lothert, Keven, Eilts, Friederike, Wolff, Michael W.

Source: Expert review of vaccines. 21(8):1029-1044

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Continuous purification of influenza A virus particles using pseudo-affinity membrane chromatography

Authors: Fortuna, A. Raquel, Taft, Florian, Villain, Louis, Wolff, Michael W., Reichl, Udo

Source: In Journal of Biotechnology 10 December 2021 342:139-148

A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling

Authors: Labisch, Jennifer J. ^*, Bollmann, Franziska, Wolff, Michael W., Pflanz, Karl

Source: In Journal of Biotechnology 20 January 2021 326:11-20

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review.

Authors: Lothert, Keven¹ (AUTHOR), Wolff, Michael W.¹ (AUTHOR) michael.wolff@lse.thm.de

Source: Membranes. Sep2023, Vol. 13 Issue 9, p770. 13p.

Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells

Authors: Eilts, Friederike, Harnischfeger, Julie, Loewe, Daniel, Wolff, Michael W., Salzig, Denise, Czermak, Peter

Source: Methods in molecular biology. 2183:367-390

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Upstream and Downstream Processes for Viral Nanoplexes as Vaccines

Authors: Lothert, Keven, Dekevic, Gregor, Loewe, Daniel, Salzig, Denise, Czermak, Peter, Wolff, Michael W.

Source: Methods in molecular biology. 2183:217-248

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Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography

Authors: Marichal-Gallardo, Pavel, Börner, Kathleen, Pieler, Michael M., Sonntag-Buck, Vera, Obr, Martin, Bejarano, David, Wolff, Michael W., Kräusslich, Hans-Georg, Reichl, Udo, Grimm, Dirk

Source: Human gene therapy. 32(17/18):959-974

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A Novel and Simplified Anion Exchange Flow‐Through Polishing Approach for the Separation of Full From Empty Adeno‐Associated Virus Capsids.

Authors: Meierrieks, Frederik, Weltken, Alisa, Pflanz, Karl, Pickl, Andreas, Graf, Benjamin, Wolff, Michael W.

Source: Biotechnology Journal; Oct2024, Vol. 19 Issue 10, p1-13, 13p

A scalable downstream process for the purification of the cell culture-derived Orf virus for human or veterinary applications

Authors: Lothert, Keven, Pagallies, Felix, Eilts, Friederike, Sivanesapillai, Arabi, Hardt, Martin, Moebus, Anna, Feger, Thomas, Amann, Ralf, Wolff, Michael W. ^*

Source: In Journal of Biotechnology 10 November 2020 323:221-230

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

Authors: Lothert, Keven, Pagallies, Felix, Feger, Thomas, Amann, Ralf, Wolff, Michael W. ^*

Source: In Journal of Biotechnology 10 November 2020 323:62-72