Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models

Authors: Seiichi Shinji, Yutaro Ogawa, Takeshi Yamada, Akihisa Matsuda, Kay Uehara, Yasuyuki Yokoyama, Goro Takahashi, Takuma Iwai, Toshimitsu Miyasaka, Shintaro Kanaka, Koki Hayashi, Yuuki Shichi, Masakazu Fujiwara, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata, Hiroshi Yoshida

Source: Scientific Reports, Vol 15, Iss 1, Pp 1-11 (2025)

Subject Terms: Colorectal adenocarcinoma, 2-dimensional culture, 3-dimensional culture, Sphere, Scanning electron microscopy, Transmission electron microscopy, Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/4c07a1a0d3c945b4b5e308831ddc7d42

Single-cell RNA sequencing to detect age-associated genes that identify senescent cells in the liver of aged mice

Authors: Yuta Doshida, Shinichi Hashimoto, Sadahiro Iwabuchi, Yuka Takino, Toshiyuki Ishiwata, Toshiro Aigaki, Akihito Ishigami

Source: Scientific Reports, Vol 13, Iss 1, Pp 1-13 (2023)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/009d14185bfc41ad962180e357571467

Spatiotemporal changes of tissue glycans depending on localization in cardiac aging

Authors: Yoko Itakura, Yasuko Hasegawa, Yurika Kikkawa, Yuina Murakami, Kosuke Sugiura, Chiaki Nagai-Okatani, Norihiko Sasaki, Mariko Umemura, Yuji Takahashi, Tohru Kimura, Atsushi Kuno, Toshiyuki Ishiwata, Masashi Toyoda

Source: Regenerative Therapy, Vol 22, Iss , Pp 68-78 (2023)

Subject Terms: Cardiac tissue, Lectin microarray, Aging, Glycan profile, Microvessels, Medicine (General), R5-920, Cytology, QH573-671

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2352320422001316; https://doaj.org/toc/2352-3204

Access URL: https://doaj.org/article/b2bed5b042084a44a29c66a2c5018059

Artificial intelligence-based analysis of time-lapse images of sphere formation and process of plate adhesion and spread of pancreatic cancer cells

Authors: Yuuki Shichi, Fujiya Gomi, Yasuko Hasegawa, Keisuke Nonaka, Seiichi Shinji, Kimimasa Takahashi, Toshiyuki Ishiwata

Source: Frontiers in Cell and Developmental Biology, Vol 11 (2023)

Subject Terms: pancreatic cancer, sphere, cell adhesion, migration, time-lapse analysis, epithelial-mesenchymal features, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fcell.2023.1290753/full; https://doaj.org/toc/2296-634X

Access URL: https://doaj.org/article/f820c6f219084e938730943ae997de86

Accelerated telomere shortening in adrenal zona reticularis in patients with prolonged critical illness

Authors: Keisuke Nonaka, Kaiyo Takubo, Junko Aida, Yoriko Watai, Akiko Komatsu, Fujiya Gomi, Yuuki Shichi, Yuto Yamazaki, Toshiyuki Ishiwata, Hironobu Sasano, Tomio Arai

Source: Frontiers in Endocrinology, Vol 14 (2023)

Subject Terms: telomere, adrenal gland, zona reticularis, prolonged critical illness, DHEA, hypoalbuminemia, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fendo.2023.1244553/full; https://doaj.org/toc/1664-2392

Access URL: https://doaj.org/article/8d3eff5b9ee346f5ba26e11f15240004

mTOR pathway as a potential therapeutic target for cancer stem cells in canine mammary carcinoma

Authors: Masaki Michishita, Kazuhiko Ochiai, Rei Nakahira, Daigo Azakami, Yukino Machida, Tomokazu Nagashima, Takayuki Nakagawa, Toshiyuki Ishiwata

Source: Frontiers in Oncology, Vol 13 (2023)

Subject Terms: cancer stem cells (CSC), dog, mammary adenocarcinoma, mTOR, sphere-formation assay, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fonc.2023.1100602/full; https://doaj.org/toc/2234-943X

Access URL: https://doaj.org/article/68c192b5ca1742e498e9e51c465cf965

Multiple cystic sphere formation from PK-8 cells in three-dimensional culture

Authors: Yuuki Shichi, Fujiya Gomi, Yoshibumi Ueda, Keisuke Nonaka, Fumio Hasegawa, Yasuko Hasegawa, Nae Hinata, Hisashi Yoshimura, Masami Yamamoto, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata

Source: Biochemistry and Biophysics Reports, Vol 32, Iss , Pp 101339- (2022)

Subject Terms: Pancreatic ductal adenocarcinoma/PDAC, E-cadherin, PK-8, Sphere, Cyst, Three-dimensional culture, Biology (General), QH301-705.5, Biochemistry, QD415-436

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S240558082200139X; https://doaj.org/toc/2405-5808

Access URL: https://doaj.org/article/5c005926cb2e4ad599fae0a0727a0d91

PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells

Authors: Norihiko Sasaki, Kazumi Hirano, Yuuki Shichi, Yoko Itakura, Toshiyuki Ishiwata, Masashi Toyoda

Source: Frontiers in Cell and Developmental Biology, Vol 10 (2022)

Subject Terms: smooth muscle cell, phenotype switching, ganglioside, polycomb repressor complex 2, enhancer of zeste homolog 2, proliferation, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fcell.2022.1003349/full; https://doaj.org/toc/2296-634X

Access URL: https://doaj.org/article/6855753eb97e46daaa44c2f9e1904133

TGF-β1 increases cellular invasion of colorectal neuroendocrine carcinoma cell line through partial epithelial-mesenchymal transition

Authors: Norihiko Sasaki, Seiichi Shinji, Yuuki Shichi, Toshiyuki Ishiwata, Tomio Arai, Takeshi Yamada, Goro Takahashi, Ryo Ohta, Hiromichi Sonoda, Akihisa Matsuda, Takuma Iwai, Kohki Takeda, Kazuhide Yonaga, Koji Ueda, Sho Kuriyama, Toshimitsu Miyasaka, Hiroshi Yoshida

Source: Biochemistry and Biophysics Reports, Vol 30, Iss , Pp 101239- (2022)

Subject Terms: Neuroendocrine carcinoma, EMT, TGF-β1, α2-integrin, Adhesion, Invasion, Biology (General), QH301-705.5, Biochemistry, QD415-436

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2405580822000395; https://doaj.org/toc/2405-5808

Access URL: https://doaj.org/article/67c15dce15f04150a384a2098a33d490

Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures

Authors: Fuuka Minami, Norihiko Sasaki, Yuuki Shichi, Fujiya Gomi, Masaki Michishita, Kozo Ohkusu-Tsukada, Masashi Toyoda, Kimimasa Takahashi, Toshiyuki Ishiwata

Source: Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/98c8eef5297c4fc69d1fbd8dca4302e7

Polyvinyl alcohol increased growth, migration, invasion, and sphere size in the PK-8 pancreatic ductal adenocarcinoma cell line

Authors: Fujiya Gomi, Norihiko Sasaki, Yuuki Shichi, Fuuka Minami, Seiichi Shinji, Masashi Toyoda, Toshiyuki Ishiwata

Source: Heliyon, Vol 7, Iss 2, Pp e06182- (2021)

Subject Terms: Pancreatic cancer, Polyvinyl alcohol, Migration, Invasion, Sphere, Cancer stem cells, Science (General), Q1-390, Social sciences (General), H1-99

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2405844021002875; https://doaj.org/toc/2405-8440

Access URL: https://doaj.org/article/10621cca69144a078c8f802ec162b1e6

Occult mucin-producing urothelial-type adenocarcinoma of the prostate with elevated serum levels of carcinoembryonic antigen and carbohydrate antigen 19-9: Report of an autopsy-proven case

Authors: Keisuke Nonaka, Yoko Matsuda, Mototsune Kakizaki, Shoichiro Takakuma, Tomoyasu Matsubara, Shigeo Murayama, Toshiyuki Ishiwata, Masashi Kameyama, Kazuto Ogura, Sumiko Kobayashi, Tomio Arai

Source: Urology Case Reports, Vol 23, Iss , Pp 6-9 (2019)

Subject Terms: Diseases of the genitourinary system. Urology, RC870-923

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2214442018303784; https://doaj.org/toc/2214-4420

Access URL: https://doaj.org/article/724f2a169ee84eafa409d9c9575f6258

Gradual telomere shortening and increasing chromosomal instability among PanIN grades and normal ductal epithelia with and without cancer in the pancreas.

Authors: Yoko Matsuda, Toshiyuki Ishiwata, Naotaka Izumiyama-Shimomura, Hideki Hamayasu, Mutsunori Fujiwara, Ken-Ichiro Tomita, Naoki Hiraishi, Ken-Ichi Nakamura, Naoshi Ishikawa, Junko Aida, Kaiyo Takubo, Tomio Arai

Source: PLoS ONE, Vol 10, Iss 2, p e0117575 (2015)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC4319908?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/84ee9315b8e14c51bcdf888cf5aaffce

Metabolite profiling in sphere-forming cells from canine mammary adenocarcinoma cell lines using gas chromatography-mass spectrometry.

Authors: Masaki MICHISHITA, Namika SAITO, Satoshi NOZAWA, Rina FURUMOTO, Takayuki NAKAGAWA, Touko SATO, Kazuhiko OCHIAI, Daigo AZAKAMI, Kinya KATAYAMA, Rei NAKAHIRA, Hiroyuki TAZAKI, Yukino MACHIDA, Toshiyuki ISHIWATA

Source: Journal of Veterinary Medical Science; 2019, Vol. 81 Issue 9, p1238-1248, 11p

Subject Terms: CANCER stem cells, CELL lines, METABOLOMICS, TIME-of-flight mass spectrometry, CELL populations, SPECTROMETRY, STEM cells, DISCRIMINANT analysis

Leydig cell tumor in an Amur tiger (Panthera tigris altaica).

Authors: Risako KAWATA, Tatsuhito II, Tatsuya HORI, Yukino MACHIDA, Kazuhiko OCHIAI, Daigo AZAKAMI, Toshiyuki ISHIWATA, Masaki MICHISHITA

Source: Journal of Veterinary Medical Science; Feb2019, Vol. 81 Issue 2, p186-189, 4p

Subject Terms: LEYDIG cells, TIGERS, MALE reproductive organs, CELL tumors, TESTIS, SYNAPTOPHYSIN

Disseminated histiocytic sarcoma with hemophagocytosis in a rabbit.

Authors: Mio ISHIMORI, Masaki MICHISHITA, Hisashi YOSHIMURA, Daigo AZAKAMI, Kazuhiko OCHIAI, Toshiyuki ISHIWATA, Kimimasa TAKAHASHI

Source: Journal of Veterinary Medical Science; Sep2017, Vol. 79 Issue 9, p1503-1506, 4p

Subject Terms: RETICULUM cell sarcoma, PHAGOCYTOSIS, RABBITS, THROMBOCYTOPENIA, RADIOSCOPIC diagnosis, ANIMAL health

Identification of tumor-initiating cells derived from two canine rhabdomyosarcoma cell lines.

Authors: Takuya Evan KISHIMOTO, Shoko YASHIMA, Rei NAKAHIRA, Eri ONOZAWA, Daigo AZAKAMI, Makoto UJIKE, Kazuhiko OCHIAI, Toshiyuki ISHIWATA, Kimimasa TAKAHASHI, Masaki MICHISHITA

Source: Journal of Veterinary Medical Science; Jul2017, Vol. 79 Issue 7, p1155-1162, 8p

Subject Terms: CANCER stem cells, STEM cells, TUMORS, RHABDOMYOSARCOMA, CELL lines

Suppression of STAT5b in pancreatic cancer cells leads to attenuated gemcitabine chemoresistance, adhesion and invasion.

Authors: HIROKI SUMIYOSHI, AKIRA MATSUSHITA, YOSHIHARU NAKAMURA, YOKO MATSUDA, TOSHIYUKI ISHIWATA, ZENYA NAITO, EIJI UCHIDA

Source: Oncology Reports; 2016, Vol. 35 Issue 6, p3216-3226, 11p

Clinicopathological Features of 15 Occult and 178 Clinical Pancreatic Ductal Adenocarcinomas in 8339 Autopsied Elderly Patients.

Authors: Yoko Matsuda, Toshiyuki Ishiwata, Shinichi Yachida, Akemi Suzuki, Yuri Hamashima, Hideki Hamayasu, Hisashi Yoshimura, Naoko Honma, Junko Aida, Kaiyo Takubo, Tomio Arai

Source: Pancreas; Feb2016, Vol. 45 Issue 2, p234-240, 7p

Systemic Administration of Small Interfering RNA Targeting Human Nestin Inhibits Pancreatic Cancer Cell Proliferation and Metastasis.

Authors: Yoko Matsuda, Toshiyuki Ishiwata, Hisashi Yoshimura, Satoshi Yamashita, Toshikazu Ushijima, Tomio Arai

Source: Pancreas; Jan2016, Vol. 45 Issue 1, p93-100, 8p