Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and calcium.

Authors: Shang-Jui Tsai^1,2,3 sam255676@gmail.com, Yijing Gong^1,2,3, Dabbs, Austin^1,2,3, Zahra, Fiddia³, Junhao Xu^1,2,3, Geske, Aleksander², Caterina, Michael J.^1,2,3, Gould, Stephen J.³ sgould@jhmi.edu

Source: Journal of Biological Chemistry. Mar2025, Vol. 301 Issue 3, p1-15. 15p.

Degron tagging of BleoR and other antibiotic-resistance genes selects for higher expression of linked transgenes and improved exosome engineering.

Authors: Shang Jui Tsai¹, Yiwei Ai¹, Chenxu Guo¹, Gould, Stephen J.¹ sgould@jhmi.edu

Source: Journal of Biological Chemistry. May2022, Vol. 298 Issue 5, p1-15. 15p.

Subject Terms: *EXOSOMES, *RECOMBINANT proteins, *GENES, *ENGINEERING, *EUKARYOTIC cells, *ANDROGEN receptors, *TRANSGENE expression

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

Authors: Shang Jui Tsai¹, Atai, Nadia A.², Cacciottolo, Mafalda², Nice, Justin², Salehi, Arjang², Chenxu Guo¹, Sedgwick, Alanna², Kanagavelu, Saravana², Gould, Stephen J.¹ sgould@jhmi.edu

Source: Journal of Biological Chemistry. Nov2021, Vol. 297 Issue 5, p1-20. 20p.

Subject Terms: *MESSENGER RNA, *SARS-CoV-2, *FLUID injection, *EXOSOMES, *SKELETAL muscle

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Choice of selectable marker affects recombinant protein expression in cells and exosomes.

Authors: Chenxu Guo¹, Fordjour, Francis K.¹, Shang Jui Tsai¹, Morrell, James C.¹, Gould, Stephen J.¹ sgould@jhmi.edu

Source: Journal of Biological Chemistry. Jul2021, Vol. 297 Issue 1, p1-13. 13p.

Subject Terms: *RECOMBINANT proteins, *PROTEIN expression, *CERCOPITHECUS aethiops, *TRANSGENE expression, *CELL lines, *BUDS

Endocytosis blocks the vesicular secretion of exosome marker proteins.

Authors: Yiwei Ai¹, Chenxu Guo¹, Garcia-Contreras, Marta¹, Sánchez B., Laura S.¹, Saftics, Andras², Shodubi, Oluwapelumi¹, Raghunandan, Shankar¹, Junhao Xu¹, Shang Jui Tsai¹, Yi Dong³, Rong Li^3,4, Jovanovic-Talisman, Tijana², Gould, Stephen J.¹ sgould@jhmi.edu

Source: Science Advances. 5/10/2024, Vol. 10 Issue 19, p1-22. 22p.

Subject Terms: *EXOSOMES, *ENDOCYTOSIS, *SECRETION, *CELL membranes, *COATED vesicles, *ADAPTOR proteins, *PROTEINS