Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units

Authors: Jyunna Yoshida, Yuki Kato, Ai Isogawa, Yoshikazu Tanaka, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, Koki Makabe

Source: Journal of Biological Engineering, Vol 18, Iss 1, Pp 1-7 (2024)

Subject Terms: Bispecific antibody, Cancer, Chain pairing, SpyCatcher, SnoopCatcher, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/1754-1611

Access URL: https://doaj.org/article/287fafc3200d410b9d597532cddc3170

A Versatile Method to Create Antibody/Split‐Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System

Authors: Yuka Tobita, Kensuke Hirano, Daimei Miura, Yuma Hatano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano

Source: Advanced Sensor Research, Vol 4, Iss 1, Pp n/a-n/a (2025)

Subject Terms: C‐reactive protein, electrochemical sensors, homogeneous detection, single‐chain fragment variables, split glucose dehydrogenases, Technology (General), T1-995, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2751-1219

Access URL: https://doaj.org/article/f5ad6cd33d21470fa02891b48c539461

Proximity‐Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus‐Containing Spots

Authors: Daimei Miura, Wakana Hayashi, Kensuke Hirano, Ikkei Sasaki, Kaori Tsukakoshi, Hidehumi Kakizoe, Satomi Asai, Christopher J. Vavricka, Hitoshi Takemae, Tetsuya Mizutani, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro, Ryutaro Asano

Source: Advanced Science, Vol 11, Iss 43, Pp n/a-n/a (2024)

Subject Terms: antibody‐enzyme complex, bispecific aptamer, homogeneous immunosensor, sequential enzymatic reactions, virus detection and visualization, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2198-3844

Access URL: https://doaj.org/article/20db616a976b4cc3afbd9d28c7e29901

Construction of IgG–Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction

Authors: Risa Yamada, Ishin Nakahara, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, Koki Makabe

Source: Scientific Reports, Vol 13, Iss 1, Pp 1-7 (2023)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/dc24f6f96ed04ebbbd62b3c686ada174

Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant

Authors: Kazuaki Hoshi, Anh Thi Tram Tu, Miwako Shobo, Karin Kettisen, Lei Ye, Leif Bülow, Yoji Hakamata, Tetsuya Furuya, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode, Tomohiko Yamazaki

Source: Nanomaterials, Vol 14, Iss 17, p 1440 (2024)

Subject Terms: hemozoin analog, heme detoxification protein, adjuvant, vaccine, Th-1 immunity, Chemistry, QD1-999

File Description: electronic resource

Relation: https://www.mdpi.com/2079-4991/14/17/1440; https://doaj.org/toc/2079-4991

Access URL: https://doaj.org/article/7c3a6cfa16e740339445f586ce23e00d

Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria

Authors: Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano

Source: Microbial Cell Factories, Vol 21, Iss 1, Pp 1-11 (2022)

Subject Terms: Biofuel, CcaS/CcaR two-component system, Cyanobacteria, Isobutanol, Light-regulated gene expression, Metabolic regulation, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/1475-2859

Access URL: https://doaj.org/article/973c53bff82b4f748f03ce5231ccfd86

Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope

Authors: Koki Makabe, Takeshi Yokoyama, Shiro Uehara, Tomomi Uchikubo-Kamo, Mikako Shirouzu, Kouki Kimura, Kouhei Tsumoto, Ryutaro Asano, Yoshikazu Tanaka, Izumi Kumagai

Source: Scientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/051aab10792249469899ed21bbfad1a2

T Cell Bispecific Antibodies: An Antibody-Based Delivery System for Inducing Antitumor Immunity

Authors: Daisuke Kamakura, Ryutaro Asano, Masahiro Yasunaga

Source: Pharmaceuticals, Vol 14, Iss 11, p 1172 (2021)

Subject Terms: T cell bispecific antibody, T-BsAb, pharmacokinetics, T cell redirection, mechanism of action, drug development, Medicine, Pharmacy and materia medica, RS1-441

File Description: electronic resource

Relation: https://www.mdpi.com/1424-8247/14/11/1172; https://doaj.org/toc/1424-8247

Access URL: https://doaj.org/article/af23fae792844ddd887492ea00c2d6d8

A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity

Authors: Aruto Sugiyama, Mitsuo Umetsu, Hikaru Nakazawa, Teppei Niide, Tomoko Onodera, Katsuhiro Hosokawa, Shuhei Hattori, Ryutaro Asano, Izumi Kumagai

Source: Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/f18cb9ec074743a0b52efdd168550715

Functional integration of protein A binding ability to antibody fragments for convenient and tag-free purification.

Authors: Atsushi Kuwahara, Misae Nazuka, Yuri Kuroki, Kohei Ito, Shunsuke Watanabe, Izumi Kumagai, Ryutaro Asano

Source: Bioengineered; Dec2023, Vol. 14 Issue 1, p1-15, 15p

Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing.

Authors: Yuki Shibuya¹, Natsuki Haga¹, Ryutaro Asano², Hikaru Nakazawa³, Takamitsu Hattori³, Daisuke Takeda¹, Aruto Sugiyama³, Reiko Kurotani¹, Izumi Kumagai³, Mitsuo Umetsu³, Koki Makabe¹, makabe@yz.yamagata-u.ac.jp

Source: PEDS: Protein Engineering, Design & Selection; Jan2017, Vol. 30 Issue 1, p15-21, 7p

Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: the case of the hEx3 diabody.

Authors: Ryutaro Asano¹, Takashi Kumagai¹, Keisuke Nagai¹, Shintaro Taki¹, Ippei Shimomura¹, Kyoko Arai¹, Hiromi Ogata¹, Mai Okada¹, Fumitaka Hayasaka¹, Hideaki Sanada¹, Takeshi Nakanishi¹, Teemu Karvonen¹, Hiroki Hayashi², Yu Katayose², Michiaki Unno², Toshio Kudo³, Mitsuo Umetsu¹, Izumi Kumagai¹ kmiz@kuma.che.tohoku.ac.jp

Source: PEDS: Protein Engineering, Design & Selection. May2013, Vol. 26 Issue 5, p359-367. 9p.

Subject Terms: *BISPECIFIC antibodies, *ANTINEOPLASTIC agents, *CELL-mediated cytotoxicity, *CANCER immunotherapy, *EPIDERMAL growth factor receptors

Preferential heterodimerization of a bispecific diabody based on a humanized anti-EGFR antibody 528.

Authors: Ryutaro Asano, Yukiko Sone, Keiko Ikoma, Hiroki Hayashi, Takeshi Nakanishi, Mitsuo Umetsu, Yu Katayose, Michiaki Unno, Toshio Kudo, Izumi Kumagai

Source: PEDS: Protein Engineering, Design & Selection. Oct2008, Vol. 21 Issue 10, p597-597. 1p.

Subject Terms: *EPIDERMAL growth factor, *ESCHERICHIA coli, *DIMERS, *IMMUNOGLOBULINS, *PROTEIN engineering