RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis

Authors: Morgan R. Hiebert, Meenu K. Sharma, Alwyn Go, Christine Bonner, Vanessa Laminman, Morag Graham, Hafid Soualhine

Source: BioTechniques, Vol 77, Iss 1, Pp 23-34 (2025)

Subject Terms: Mycobacterium tuberculosis, next-generation sequencing, RNA extraction, RNA-sequencing, transcriptomics, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818

Access URL: https://doaj.org/article/8a0cfaa578f54bee9db01dfabac02791

Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits

Authors: Sukoluhle Dube, Sharefa Al-Mannai, Li Liu, Sara Tomei, Satanay Hubrack, Shimaa Sherif, Ayesha Jabeen, Eiman I. Ahmed, Apryl Sanchez, William Mifsud, Davide Bedognetti, Wouter Hendrickx, Christophe M. Raynaud

Source: Journal of Translational Medicine, Vol 23, Iss 1, Pp 1-11 (2025)

Subject Terms: FFPE RNA extraction kit, RNA quality, RNA quantity, Medicine

File Description: electronic resource

Relation: https://doaj.org/toc/1479-5876

Access URL: https://doaj.org/article/d14a48c807de43e79e8e6ddd68cab05c

Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device

Authors: Shui-Xian Guo, Qing Zhang, Nan-Nan Bai, Pei-Yao Yue, Jing-Ping Niu, Cong-Cong Yin, Ai-Qin Yue, Wei-Jun Du, Jin-Zhong Zhao

Source: Frontiers in Microbiology, Vol 15 (2025)

Subject Terms: soybean, soybean mosaic virus, virulent strain SC7, magnetic beads-based RNA extraction method, LAMP, lateral flow device, Microbiology, QR1-502

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmicb.2024.1478218/full; https://doaj.org/toc/1664-302X

Access URL: https://doaj.org/article/c3d97867fa5445d4b9227a880242a409

A molecular detection method for HBV pgRNA without RNA extraction based on nucleic acid hybridization

Authors: Hao, Xiao ^a, Yuan, Min ^b, Li, Yanmei ^b, Li, Ruirui ^a, Li, Xuejing ^{b, ⁎}, Yao, Meixue ^{c, ⁎}

Source: In Diagnostic Microbiology & Infectious Disease January 2025 111(1)

An efficient and effective RNA extraction protocol for ferns

Authors: Jessie A. Pelosi, Ruth Davenport, W. Brad Barbazuk, Emily B. Sessa, Li‐Yaung Kuo

Source: Applications in Plant Sciences, Vol 12, Iss 6, Pp n/a-n/a (2024)

Subject Terms: cetyltrimetylammonium bromide (CTAB), ferns, RNA extraction, secondary compounds, Biology (General), QH301-705.5, Botany, QK1-989

File Description: electronic resource

Relation: https://doaj.org/toc/2168-0450

Access URL: https://doaj.org/article/0815bfdeedae41cfaeb3c0af6f3341d9

An optimised bead beating RNA extraction method for tough-to-lyse gram-positive bacteria

Authors: Scanlon, Killian ^{a, b}, Ross, R.Paul ^{a, b}, Hill, Colin ^{a, b, ⁎}

Source: In The Microbe June 2025 7

Evaluation and Standardization of RNA Extractions with Quality for RNA-Seq for Balamuthia mandrillaris

Authors: Leobardo Daniel Gonzalez-Zuñiga, Libia Zulema Rodriguez-Anaya, Jose Reyes Gonzalez-Galaviz, Abraham Cruz-Mendívil, Fernando Lares-Villa, Luis Fernando Lares-Jiménez

Source: Parasitologia, Vol 4, Iss 2, Pp 199-208 (2024)

Subject Terms: Balamuthia mandrillaris, RNA extraction, free-living amoeba, RNA integrity number, transcriptomics, Infectious and parasitic diseases, RC109-216, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.mdpi.com/2673-6772/4/2/17; https://doaj.org/toc/2673-6772

Access URL: https://doaj.org/article/2d611d2a70e944ab9ecdd52622a87c18

A modified CTAB method for the extraction of high-quality RNA from mono-and dicotyledonous plants rich in secondary metabolites

Authors: Tibor Kiss, Zoltán Karácsony, Adrienn Gomba-Tóth, Kriszta Lilla Szabadi, Zsolt Spitzmüller, Júlia Hegyi-Kaló, Thomas Cels, Margot Otto, Richárd Golen, Ádám István Hegyi, József Geml, Kálmán Zoltán Váczy

Source: Plant Methods, Vol 20, Iss 1, Pp 1-7 (2024)

Subject Terms: CTAB, RNA extraction, qRT-PCR, Polysaccharide and polyphenolic rich plants, Plant culture, SB1-1110, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/1746-4811

Access URL: https://doaj.org/article/70821cd2f3d04228a56c7a836072206d

Optimized SDS-Based Protocol for High-Quality RNA Extraction from Musa spp.

Authors: Kishan Saha, Onyinye C. Ihearahu, L. H. Stevenson Naitchede, Supriyo Ray, George Ude

Source: Methods and Protocols, Vol 8, Iss 1, p 21 (2025)

Subject Terms: RNA extraction method, SDS, CTAB, cDNA, Musa spp., Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.mdpi.com/2409-9279/8/1/21; https://doaj.org/toc/2409-9279

Access URL: https://doaj.org/article/20d865f512f24e89a714b24dd867f7be

Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Authors: Matejkova, Nikola ^{a, 1}, Smela, Denisa ^{a, 1}, Beranek, Martin ^{b, c, 2}, Capek, Jan ^a, Michalcova, Lucie ^a, Michalkova, Lenka ^d, Svoboda, Jakub ^d, Skeren, Marek ^d, Svobodova, Zuzana ^e, Sopha, Hanna ^{f, g}, Macak, Jan M. ^{f, g}, Korecka, Lucie ^a, Pacinkova, Anna ^h, Gancarcikova, Marketa ^b, Bolehovska, Radka ⁱ, Koblizek, Vladimir ^j, Bilkova, Zuzana ^{a, ⁎}

Source: In Microchemical Journal November 2024 206

Turbolysis: A low-cost, small footprint alternative to commercial bead beaters for cell lysis

Authors: Jason D. Limberis, John Z. Metcalfe

Source: HardwareX, Vol 19, Iss , Pp e00576- (2024)

Subject Terms: Cell lysis, Tuberculosis, DNA extraction, RNA extraction, Science (General), Q1-390

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2468067224000701; https://doaj.org/toc/2468-0672

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Optimizing total RNA extraction method for human and mice samples

Authors: Yumei Zeng, Xiaoxue Tang, Jinwen Chen, Xi Kang, Dazhang Bai

Source: PeerJ, Vol 12, p e18072 (2024)

Subject Terms: Total RNA extraction method, TRIzol reagent, The TRIzol method, GITC-T method, SDS-T method, Medicine, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://peerj.com/articles/18072.pdf; https://peerj.com/articles/18072/; https://doaj.org/toc/2167-8359

Access URL: https://doaj.org/article/bf85f80e9aeb45c8b5c69cb32d357871

A single workflow for multi-species blood transcriptomics

Authors: Elody Orcel, Hayat Hage, May Taha, Noémie Boucher, Emilie Chautard, Virginie Courtois, Adrien Saliou

Source: BMC Genomics, Vol 25, Iss 1, Pp 1-16 (2024)

Subject Terms: Preclinical models, Clinical models, Blood samples, RNA extraction, Library preparation, Total RNA sequencing, Biotechnology, TP248.13-248.65, Genetics, QH426-470

File Description: electronic resource

Relation: https://doaj.org/toc/1471-2164

Access URL: https://doaj.org/article/d5c4a753fb8249b19f6ee4c60a79d693

Development of Magnetic-Silica Particles and In-house Buffers Kit for SARS-CoV-2 and CDV RNA Extraction

Authors: Ahadi Damar Prasetya, Muflikhah Muflikhah, Wildan Zakiah Lubis, Andon Insani, Grace Tjungirai Sulungbudi, Mujamilah Mujamilah, Uus Saepulloh

Source: Indonesian Journal of Chemistry, Vol 24, Iss 1, Pp 81-93 (2024)

Subject Terms: buffer kit, canine distemper virus, magnetic-silica, rna extraction, sars-cov-2, Chemistry, QD1-999

File Description: electronic resource

Relation: https://jurnal.ugm.ac.id/ijc/article/view/83804; https://doaj.org/toc/1411-9420; https://doaj.org/toc/2460-1578

Access URL: https://doaj.org/article/6826c717c8c9462eaeac962a5e2e6ed9

Proof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation Methods

Authors: Patrick Hannibal Dalsbo Petersen, Jaslin Pallikkunnath James, Lene Buhl Riis, Claus Kim Høgdall, Estrid Vilma Høgdall

Source: Methods and Protocols, Vol 7, Iss 6, p 101 (2024)

Subject Terms: FFPE, RNA extraction, KingFisher, automation, AutoLys, deparaffinization, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.mdpi.com/2409-9279/7/6/101; https://doaj.org/toc/2409-9279

Access URL: https://doaj.org/article/c1cbf4168d354af887d665b9af5c91ff

Evaluation of a new automated viral RNA extraction platform for hepatitis A virus and human norovirus in testing of berries, lettuce, and oysters

Authors: Trudel-Ferland, Mathilde ^a, Collard, Marie-Ève ^a, Goulet-Beaulieu, Valérie ^a, Jubinville, Eric ^a, Hamon, Fabienne ^b, Jean, Julie ^{a, ⁎}

Source: In International Journal of Food Microbiology 2 May 2024 416

A method for measuring mitochondrial DNA copy number in pediatric populations

Authors: Simran Maggo, Liam Y. North, Aime Ozuna, Dejerianne Ostrow, Yander R. Grajeda, Hesamedin Hakimjavadi, Jennifer A. Cotter, Alexander R. Judkins, Pat Levitt, Xiaowu Gai

Source: Frontiers in Pediatrics, Vol 12 (2024)

Subject Terms: mitochondrial DNA copy number, adversity, lpWGS, adverse childhood experience (ACE), DNA RNA extraction, Pediatrics, RJ1-570

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fped.2024.1401737/full; https://doaj.org/toc/2296-2360

Access URL: https://doaj.org/article/c4a5d9846d754dc9bc7df7a2c19feabc

Assessing the impact of storage conditions on RNA from human saliva and its application to the identification of mRNA biomarkers for asthma

Authors: Poorna Manasa Bhamidimarri, David Fuentes, Laila Salameh, Bassam Mahboub, Rifat Hamoudi

Source: Frontiers in Molecular Biosciences, Vol 11 (2024)

Subject Terms: salivary RNA, degradation, gene expression, diagnosis, RNA extraction, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmolb.2024.1363897/full; https://doaj.org/toc/2296-889X

Access URL: https://doaj.org/article/aa682b916c23410ba02577c76c20072c

CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis

Authors: Scott A. Schactler, Stephen J. Scheuerman, Andrea Lius, William A. Altemeier, Dowon An, Thomas J. Matula, Michal Mikula, Maria Kulecka, Oleg Denisenko, Daniel Mar, Karol Bomsztyk

Source: BMC Genomics, Vol 24, Iss 1, Pp 1-16 (2023)

Subject Terms: CryoGrid for tissues cryostoring and sampling, Proteinase K, PIXUL RNA extraction, RNA sequencing, Transcriptomics, Biotechnology, TP248.13-248.65, Genetics, QH426-470

File Description: electronic resource

Relation: https://doaj.org/toc/1471-2164

Access URL: https://doaj.org/article/cd39d825b9a040c79fcfc18106dd7838

DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

Authors: Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri

Source: Plant Methods, Vol 19, Iss 1, Pp 1-13 (2023)

Subject Terms: CTAB, Conocarpus erectus, Date palm, Prosopis cineraria, RNA extraction, TRIzol, Plant culture, SB1-1110, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/1746-4811

Access URL: https://doaj.org/article/ce6bb19f8a864c07a8d9b4b3c5bab770