The lack of trade-off between conformational stability and binding affinity in a nanobody with therapeutic potential for a misfolding disease

Authors: Gómez-Mulas, Atanasio ^a, Naganathan, Athi N. ^b, Pey, Angel L. ^{c, ⁎}

Source: In International Journal of Biological Macromolecules January 2025 284 Part 1

Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzymeElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3lc00176h

Authors: Doppler, Diandra, Sonker, Mukul, Egatz-Gomez, Ana, Grieco, Alice, Zaare, Sahba, Jernigan, Rebecca, Meza-Aguilar, Jose Domingo, Rabbani, Mohammad T., Manna, Abhik, Alvarez, Roberto C., Karpos, Konstantinos, Cruz Villarreal, Jorvani, Nelson, Garrett, Yang, Jay-How, Carrion, Jackson, Morin, Katherine, Ketawala, Gihan K., Pey, Angel L., Ruiz-Fresneda, Miguel Angel, Pacheco-Garcia, Juan Luis, Hermoso, Juan A., Nazari, Reza, Sierra, Raymond, Hunter, Mark S., Batyuk, Alexander, Kupitz, Christopher J., Sublett, Robert E., Lisova, Stella, Mariani, Valerio, Boutet, Sébastien, Fromme, Raimund, Grant, Thomas D., Botha, Sabine, Fromme, Petra, Kirian, Richard A., Martin-Garcia, Jose Manuel, Ros, Alexandra

Source: Lab on a chip. 23(13):3016-3033

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100186146046.0x000001&indx=1&recIds=ETOCvdc_100186146046.0x000001

Counterintuitive structural and functional effects due to naturally occurring mutations targeting the active site of the disease‐associated NQO1 enzyme*

Authors: Pacheco‐García, Juan Luis, Anoz‐Carbonell, Ernesto, Loginov, Dmitry S., Kavan, Daniel, Salido, Eduardo, Man, Petr, Medina, Milagros, Pey, Angel L.

Source: FEBS journal. 290(7):1855-1873

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100182037394.0x000001&indx=1&recIds=ETOCvdc_100182037394.0x000001

Different phenotypic outcome due to site-specific phosphorylation in the cancer-associated NQO1 enzyme studied by phosphomimetic mutations

Authors: Pacheco-Garcia, Juan Luis, Anoz-Carbonell, Ernesto, Loginov, Dmitry S., Vankova, Pavla, Salido, Eduardo, Man, Petr, Medina, Milagros, Palomino-Morales, Rogelio, Pey, Angel L.

Source: In Archives of Biochemistry and Biophysics 30 October 2022 729

A single evolutionarily divergent mutation determines the different FAD‐binding affinities of human and rat NQO1 due to site‐specific phosphorylation

Authors: Pacheco‐Garcia, Juan Luis, Loginov, Dmitry, Rizzuti, Bruno, Vankova, Pavla, Neira, Jose L., Kavan, Daniel, Mesa‐Torres, Noel, Guzzi, Rita, Man, Petr, Pey, Angel L.

Source: FEBS letters. 596(1):29-41

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100138906927.0x000001&indx=1&recIds=ETOCvdc_100138906927.0x000001

New salicylic acid derivatives, double inhibitors of glycolate oxidase and lactate dehydrogenase, as effective agents decreasing oxalate production

Authors: Moya-Garzon, Maria Dolores, Rodriguez-Rodriguez, Barbara, Martin-Higueras, Cristina, Franco-Montalban, Francisco, Fernandes, Miguel X., Gomez-Vidal, Jose A., Pey, Angel L., Salido, Eduardo, Diaz-Gavilan, Monica

Source: In European Journal of Medicinal Chemistry 5 July 2022 237

Structural basis of the pleiotropic and specific phenotypic consequences of missense mutations in the multifunctional NAD(P)H:quinone oxidoreductase 1 and their pharmacological rescue

Authors: Pacheco-Garcia, Juan Luis, Anoz-Carbonell, Ernesto, Vankova, Pavla, Kannan, Adithi, Palomino-Morales, Rogelio, Mesa-Torres, Noel, Salido, Eduardo, Man, Petr, Medina, Milagros, Naganathan, Athi N., Pey, Angel L.

Source: In Redox Biology October 2021 46

Intrinsically disordered protein NUPR1 binds to the armadillo-repeat domain of Plakophilin 1

Authors: Santofimia-Castaño, Patricia, Rizzuti, Bruno, Pey, Angel L., Fárez-Vidal, María Esther, Iovanna, Juan L., Neira, José L.

Source: In International Journal of Biological Macromolecules 15 February 2021 170:549-560

Phosphorylation of cytosolic hPGK1 affects protein stability and ligand binding: implications for its subcellular targeting in cancer.

Authors: Pacheco‐García, Juan Luis, Cano‐Muñoz, Mario, Loginov, Dmitry S., Vankova, Pavla, Man, Petr, Pey, Angel L.

Source: FEBS Journal; Nov2024, Vol. 291 Issue 21, p4775-4795, 21p

Subject Terms: PHOSPHOGLYCERATE kinase, BINDING sites, STRUCTURAL dynamics, PROTEIN kinases, MOLECULAR dynamics, PROTEIN stability, LIGAND binding (Biochemistry)

Warfarin increases thermal resistance of albumin through stabilization of the protein lobe that includes its binding site

Authors: Rizzuti, Bruno, Bartucci, Rosa, Pey, Angel L., Guzzi, Rita

Source: In Archives of Biochemistry and Biophysics 15 November 2019 676

NQO1: A target for the treatment of cancer and neurological diseases, and a model to understand loss of function disease mechanisms

Authors: Beaver, Sarah K., Mesa-Torres, Noel, Pey, Angel L., Timson, David J.

Source: In BBA - Proteins and Proteomics July-August 2019 1867(7-8):663-676

Anion-specific interaction with human NQO1 inhibits flavin binding

Authors: Pey, Angel L.

Source: In International Journal of Biological Macromolecules 1 April 2019 126:1223-1233

Phosphorylation compromises FAD binding and intracellular stability of wild-type and cancer-associated NQO1: Insights into flavo-proteome stability

Authors: Medina-Carmona, Encarnación, Rizzuti, Bruno, Martín-Escolano, Rubén, Pacheco-García, Juan Luis, Mesa-Torres, Noel, Neira, José L., Guzzi, Rita, Pey, Angel L.

Source: In International Journal of Biological Macromolecules 15 March 2019 125:1275-1288

Biophysical and functional perturbation analyses at cancer-associated P187 and K240 sites of the multifunctional NADP(H):quinone oxidoreductase 1

Authors: Pey, Angel L.

Source: In International Journal of Biological Macromolecules 15 October 2018 118 Part B:1912-1923

Systemic Alanine Glyoxylate Aminotransferase mRNA Improves Glyoxylate Metabolism in a Mouse Model of Primary Hyperoxaluria Type 1

Authors: Kukreja, Anjli, Lasaro, Melissa, Cobaugh, Christian, Forbes, Chris, Tang, Jian-Ping, Gao, Xiang, Martin-Higueras, Cristina, Pey, Angel L., Salido, Eduardo, Sobolov, Susan, Subramanian, Romesh R.

Source: Nucleic acid therapeutics. 29(2):104-113

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100083621904.0x000001&indx=1&recIds=ETOCvdc_100083621904.0x000001

Structural and functional insights on the roles of molecular chaperones in the mistargeting and aggregation phenotypes associated with primary hyperoxaluria type I

Authors: Fernández-Higuero, José Ángel, Betancor-Fernández, Isabel, Mesa-Torres, Noel, Muga, Arturo, Salido, Eduardo, Pey, Angel L.

Source: Advances in protein chemistry and structural biology. 114:119-152

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN618768205&indx=1&recIds=ETOCRN618768205

Correction of Kinetic and Stability Defects by Tetrahydrobiopterin in Phenylketonuria Patients with Certain Phenylalanine Hydroxylase Mutations

Authors: Erlandsen, Heidi, Pey, Angel L., Gámez, Alejandra, Pérez, Belén, Desviat, Lourdes R., Aguado, Cristina, Koch, Richard, Surendran, Sankar, Tyring, Stephen, Matalon, Reuben, Scriver, Charles R., Ugarte, Magdalena, Martínez, Aurora, Stevens, Raymond C., Beutler, Ernest

Source: Proceedings of the National Academy of Sciences of the United States of America, 2004 Nov . 101(48), 16903-16908.

Access URL: https://www.jstor.org/stable/3373927

Intrinsically disordered chromatin protein NUPR1 binds to the C-terminal region of Polycomb RING1B

Authors: Santofimia-Castaño, Patricia, Rizzuti, Bruno, Pey, Ángel L., Soubeyran, Philippe, Vidal, Miguel, Urrutia, Raúl, Iovanna, Juan L., Neira, José L.

Source: Proceedings of the National Academy of Sciences of the United States of America, 2017 Aug 01. 114(31), E6332-E6341.

Access URL: https://www.jstor.org/stable/26487240

Structural dynamics and functional cooperativity of human NQO1 by ambient temperature serial crystallography and simulations.

Authors: Grieco, Alice, Boneta, Sergio, Gavira, José A., Pey, Angel L., Basu, Shibom, Orlans, Julien, Sanctis, Daniele de, Medina, Milagros, Martin‐Garcia, Jose Manuel

Source: Protein Science: A Publication of the Protein Society; Apr2024, Vol. 33 Issue 4, p1-22, 22p

Structural Basis of the Oncogenic Interaction of Phosphatase PRL-1 with the Magnesium Transporter CNNM2

Authors: Giménez-Mascarell, Paula, Oyenarte, Iker, Hardy, Serge, Breiderhoff, Tilman, Stuiver, Marchel, Kostantin, Elie, Diercks, Tammo, Pey, Angel L., Ereño-Orbea, June, Martínez-Chantar, María Luz, Khalaf-Nazzal, Reham, Claverie-Martin, Felix, Müller, Dominik, Tremblay, Michel L., Martínez-Cruz, Luis Alfonso

Source: In Journal of Biological Chemistry 20 January 2017 292(3):786-801