Standardization, validation, and comparative evaluation of a convenient surrogate recombinant vesicular stomatitis virus plaque reduction test for quantification of Hantaan orthohantavirus (HTNV) neutralizing antibodies

Authors: Jing Wei, Hui Zhang, Jiawei Pei, Qiqi Yang, Yuan Wang, Xiaolei Jin, He Liu, Liang Zhang, Hongwei Ma, Linfeng Cheng, Yangchao Dong, Yingfeng Lei, Yinlan Bai, Zhikai Xu, Pengbo Yu, Fanglin Zhang, Wei Ye

Source: Virology Journal, Vol 22, Iss 1, Pp 1-13 (2025)

Subject Terms: Hantaan orthohantavirus (HTNV), Hemorrhagic fever with renal syndrome (HFRS), Vesicular stomatitis virus (VSV), Neutralizing antibodies (NAbs), Focus reduction neutralization test (FRNT), Plaque reduction neutralization test (PRNT), Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: https://doaj.org/toc/1743-422X

Access URL: https://doaj.org/article/62713286a1eb480f9577a7af71c1ec6b

Evaluation of three alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to dengue virus serotype 2

Authors: Vanessa Shi Li Goh, Christopher Chong Wei Ang, Swee Ling Low, Pei Xuan Lee, Yin Xiang Setoh, Judith Chui Ching Wong

Source: Virology Journal, Vol 21, Iss 1, Pp 1-10 (2024)

Subject Terms: Plaque reduction neutralization test, PRNT, Serology, Neutralizing antibodies, Dengue, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: https://doaj.org/toc/1743-422X

Access URL: https://doaj.org/article/3da568e388094bd2837a9ef4877fb8fc

Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment

Authors: Kong, Hyun-Jung, Kim, You-Jin, Kim, Dokeun, Hwang, Yun-Ho ^⁎

Source: In Journal of Immunological Methods January 2025 536

Comparative Analysis of Hemagglutination Inhibition and Plaque Reduction Neutralization Tests for Japanese Encephalitis Virus Antibody Detection

Authors: Cui Li, Jianqing Wan, Deli Wang, Lu Xiao, Xuni Li, Cunshuai Zhang, Zhao Wang

Source: Viruses, Vol 17, Iss 1, p 104 (2025)

Subject Terms: Japanese encephalitis, PRNT, hemagglutination inhibition, antibody, Microbiology, QR1-502

File Description: electronic resource

Relation: https://www.mdpi.com/1999-4915/17/1/104; https://doaj.org/toc/1999-4915

Access URL: https://doaj.org/article/3c5e5bef4b364f148d87467116dd5a3a

Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity

Authors: Yinyi Yu, Krystal Meza, Chase Colbert, Daniel F. Hoft, Anna Jaunarajs, Azra Blazevic, Sharon E. Frey, Getahun Abate

Source: Vaccines, Vol 13, Iss 1, p 27 (2024)

Subject Terms: mpox, vaccinia, FRNT, microneutralization, PRNT, IFN-γ ELISPOT, Medicine

File Description: electronic resource

Relation: https://www.mdpi.com/2076-393X/13/1/27; https://doaj.org/toc/2076-393X

Access URL: https://doaj.org/article/9527883aebd04b53ae10a87971bc4a0f

Neurological Manifestation Associated with Chikungunya Infection in a Pediatric Patient from Itacoatiara, Brazilian Amazon: A Case Report

Authors: Samuel Benjamin Aguiar de Oliveira, Barbara Aparecida Chaves, Maurício Teixeira Lima, Alexandre Vilhena Silva-Neto, Jady Shayenne Mota Cordeiro, Wuelton Marcelo Monteiro, Michele de Souza Bastos, Vanderson de Souza Sampaio

Source: Viruses, Vol 16, Iss 11, p 1658 (2024)

Subject Terms: chikungunya, pediatrics, encephalitis, PRNT, case report, Microbiology, QR1-502

File Description: electronic resource

Relation: https://www.mdpi.com/1999-4915/16/11/1658; https://doaj.org/toc/1999-4915

Access URL: https://doaj.org/article/9cd1c52da2ed40a5a26544eb2550cc64

Evaluation of humoral immune response after yellow fever infection: an observational study on patients from the 2017–2018 sylvatic outbreak in Brazil

Authors: Andreza Parreiras Gonçalves, Letícia Trindade Almeida, Izabela Maurício de Rezende, Jordana Rodrigues Barbosa Fradico, Leonardo Soares Pereira, Dario Brock Ramalho, Marcelo Antônio Pascoal Xavier, Carlos Eduardo Calzavara Silva, Thomas P. Monath, Angelle Desiree LaBeaud, Betania Paiva Drumond, Ana Carolina Campi-Azevedo, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Pedro Augusto Alves

Source: Microbiology Spectrum, Vol 12, Iss 5 (2024)

Subject Terms: yellow fever, PRNT, neutralizing antibodies, wild-type strain, 17DD YFV strain, late relapsing hepatitis after yellow fever, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/2165-0497

Access URL: https://doaj.org/article/74460047409c4896a13dac51f230339f

Evolution of neutralizing antibodies and cross-activity against different variants of SARS-CoV-2 in patients recovering from COVID-19

Authors: Wang-Da Liu, Jann-Tay Wang, Tai-Ling Chao, Si-Man Ieong, Ya-Min Tsai, Po-Hsien Kuo, Ming-Jui Tsai, Yi-Jie Chen, Guei-Chi Li, Shu-Yuan Ho, Hui-Hou Chen, Yu-Shan Huang, Chien-Ching Hung, Yee-Chun Chen, Sui-Yuan Chang, Shan-Chwen Chang

Source: Journal of the Formosan Medical Association, Vol 122, Iss 8, Pp 714-722 (2023)

Subject Terms: Plaque reduction neutralization test (PRNT), Humoral immunity, Spike protein, Variant of concern, Delta variant, B.1.617.2, Medicine (General), R5-920

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S0929664622004363; https://doaj.org/toc/0929-6646

Access URL: https://doaj.org/article/4bc0e23eccf9454b8fa54efd270cdfb7

The burden of dengue and force of infection among children in Kerala, India; seroprevalence estimates from Government of Kerala-WHO Dengue studyResearch in context

Authors: Pillaveetil Sathyadas Indu, Thekkumkara Surendran Anish, Sujatha Chintha, Gnanaseelan Kanakamma Libu, Lawrence Tony, Nalinakshan Sudha Siju, Easwaran Sreekumar, Asokan Santhoshkumar, Reghukumar Aravind, Karunakaran Lalithabai Saradadevi, Sahadevan Sunija, Jaichand Johnson, Madhukumar Geethakumari Anupriya, Thomas Mathew, Kalathil Joseph Reena, Vasu Meenakshy, Premaletha Namitha, Narendran Pradeep Kumar, Roop Kumari, Ahmed Jamsheed Mohamed, Bhupender Nagpal, Swarup Sarkar, Rajeev Sadanandan, Raman Velayudhan

Source: The Lancet Regional Health - Southeast Asia, Vol 22, Iss , Pp 100337- (2024)

Subject Terms: Burden of dengue: force of infection, Seroprevalence of dengue, Validation of IgG against PRNT, Plaque Reduction Neutralisation Test, Govt of Kerala-WHO Dengue study, Serprevalence of dengue in children, Seroconversion in dengue, Public aspects of medicine, RA1-1270

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S277236822300197X; https://doaj.org/toc/2772-3682

Access URL: https://doaj.org/article/f37c3d2b818447f39e936bf93f1fbaba

Oropouche Virus Exposure in Febrile Patients during Chikungunya Virus Introduction in the State of Amapá, Amazon Region, Brazil

Authors: Raquel Curtinhas de Lima, Helver Gonçalves Dias, Thiara Manuele Alves de Souza, Débora Familiar-Macedo, Edcelha D’Athaide Ribeiro, Valmir Corrêa e Corrêa, Alex Pauvolid-Corrêa, Elzinandes Leal de Azeredo, Flávia Barreto dos Santos

Source: Pathogens, Vol 13, Iss 6, p 469 (2024)

Subject Terms: surveillance, PRNT, arbovirus, RT-qPCR, Medicine

File Description: electronic resource

Relation: https://www.mdpi.com/2076-0817/13/6/469; https://doaj.org/toc/2076-0817

Access URL: https://doaj.org/article/bc8b92108dd94900960998d8f604ace0

Persistence of SARS-CoV-2 neutralizing antibodies longer than 13 months in naturally infected, captive white-tailed deer (Odocoileus virginianus), Texas

Authors: Sarah A. Hamer, Chase Nunez, Christopher M. Roundy, Wendy Tang, Logan Thomas, Jack Richison, Jamie S. Benn, Lisa D. Auckland, Terry Hensley, Walter E. Cook, Alex Pauvolid-Corrêa, Gabriel L. Hamer

Source: Emerging Microbes and Infections, Vol 11, Iss 1, Pp 2112-2115 (2022)

Subject Terms: SARS-CoV-2, Odocoileus virginianus, white-tailed deer, PRNT, spillover, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/2222-1751

Access URL: https://doaj.org/article/333bd710bbde4e0d9a42b1f138408f73

Investigation of Yellow Fever Virus at the Human–Animal Interface after a Zika Virus Outbreak in Midwest Brazil

Authors: Lis Alvarez Baranowski, Helver Gonçalves Dias, Débora Familiar-Macedo, Gilberto Sabino-Santos, Heitor Miraglia Herrera, Renata Dezengrini Slhessarenko, Flávia Barreto dos Santos, Alex Pauvolid-Corrêa

Source: Microorganisms, Vol 12, Iss 3, p 594 (2024)

Subject Terms: non-human primates, PRNT, state of Mato Grosso, state of Mato Grosso do Sul, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.mdpi.com/2076-2607/12/3/594; https://doaj.org/toc/2076-2607

Access URL: https://doaj.org/article/f4efa3fce8084420ad8be745f21ab236

Plaque Reduction Neutralization Test (PRNT) Accuracy in Evaluating Humoral Immune Response to SARS-CoV-2

Authors: Ingrid Siciliano Horbach, Adriana de Souza Azevedo, Waleska Dias Schwarcz, Nathalia dos Santos Alves, Brenda de Moura Dias, Bruno Pimenta Setatino, Luma da Cruz Moura, Ariane Faria de Souza, Caio Bidueira Denani, Stephanie Almeida da Silva, Thiago Goes Pimentel, Victor de Oliveira Silva Ferreira, Tamiris Azamor, Ana Paula Dinis Ano Bom, Maria da Penha Gomes Gouvea, José Geraldo Mill, Valéria Valim, Jessica Polese, Ana Carolina Campi-Azevedo, Vanessa Peruhype-Magalhães, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho, Sheila Maria Barbosa de Lima, Ivanildo Pedro de Sousa Junior

Source: Diseases, Vol 12, Iss 1, p 29 (2024)

Subject Terms: SARS-CoV-2, COVID-19, Wuhan, Omicron, neutralizing antibodies, PRNT, Medicine

File Description: electronic resource

Relation: https://www.mdpi.com/2079-9721/12/1/29; https://doaj.org/toc/2079-9721

Access URL: https://doaj.org/article/032051c335f14b92b57e167095e2e442

Neutralization of SARS-CoV-2 Omicron and Delta Variants in Relation to Vaccine-Induced Antibody Levels in Kidney Transplant Recipients and Healthy Controls

Authors: Rune M. Pedersen, Line L. Bang, Ditte S. Tornby, Helene Kierkegaard, Anna C. Nilsson, Isik S. Johansen, Thomas V. Sydenham, Thøger G. Jensen, Ulrik S. Justesen, Claus Bistrup, Thomas E. Andersen

Source: Microbiology Spectrum, Vol 10, Iss 5 (2022)

Subject Terms: COVID-19, Delta, kidney transplant recipients, Omicron, PRNT, SARS-CoV-2, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/2165-0497

Access URL: https://doaj.org/article/facbe751f24e43d1a16776a2b7247cd0

A High-Throughput Yellow Fever Neutralization Assay

Authors: Madina Rasulova, Thomas Vercruysse, Jasmine Paulissen, Catherina Coun, Vanessa Suin, Leo Heyndrickx, Ji Ma, Katrien Geerts, Jolien Timmermans, Niraj Mishra, Li-Hsin Li, Dieudonné Buh Kum, Lotte Coelmont, Steven Van Gucht, Hadi Karimzadeh, Julia Thorn-Seshold, Simon Rothenfußer, Kevin K. Ariën, Johan Neyts, Kai Dallmeier, Hendrik Jan Thibaut

Source: Microbiology Spectrum, Vol 10, Iss 3 (2022)

Subject Terms: yellow fever virus, serodiagnosis, reporter virus, PRNT, neutralization assay, high-throughput, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/2165-0497

Access URL: https://doaj.org/article/61bcd96f42ef42ad9b0ace605b4b0c42

Rapid Generation of In-House Serological Assays Is Comparable to Commercial Kits Critical for Early Response to Pandemics: A Case With SARS-CoV-2

Authors: Heidi Auerswald, Chanreaksmey Eng, Sokchea Lay, Saraden In, Sokchea Eng, Hoa Thi My Vo, Charya Sith, Sokleaph Cheng, Gauthier Delvallez, Vann Mich, Ngy Meng, Ly Sovann, Kraing Sidonn, Jessica Vanhomwegen, Tineke Cantaert, Philippe Dussart, Veasna Duong, Erik A. Karlsson

Source: Frontiers in Medicine, Vol 9 (2022)

Subject Terms: SARS-CoV-2, serology, ELISA, PRNT, immunoassay, Medicine (General), R5-920

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmed.2022.864972/full; https://doaj.org/toc/2296-858X

Access URL: https://doaj.org/article/a01b0423301c41eca8f936bc0c38c3f3

Tick-Borne Encephalitis Virus Prevalence in Sheep, Wild Boar and Ticks in Belgium

Authors: Nadjah Radia Adjadj, Muriel Vervaeke, Charlotte Sohier, Mickaël Cargnel, Nick De Regge

Source: Viruses, Vol 14, Iss 11, p 2362 (2022)

Subject Terms: prevalence, sheep, wild boar, tick-borne encephalitis, ticks, PRNT, Microbiology, QR1-502

File Description: electronic resource

Relation: https://www.mdpi.com/1999-4915/14/11/2362; https://doaj.org/toc/1999-4915

Access URL: https://doaj.org/article/d5e07fd3344240e0a3e43de828583c0c

High Efficacy of Therapeutic Equine Hyperimmune Antibodies Against SARS-CoV-2 Variants of Concern

Authors: Andres Moreira-Soto, Mauricio Arguedas, Hebleen Brenes, Willem Buján, Eugenia Corrales-Aguilar, Cecilia Díaz, Ann Echeverri, Marietta Flores-Díaz, Aarón Gómez, Andrés Hernández, María Herrera, Guillermo León, Román Macaya, Arne Kühne, José Arturo Molina-Mora, Javier Mora, Alfredo Sanabria, Andrés Sánchez, Laura Sánchez, Álvaro Segura, Eduardo Segura, Daniela Solano, Claudio Soto, Jennifer L. Stynoski, Mariángela Vargas, Mauren Villalta, Chantal B. E. M. Reusken, Christian Drosten, José María Gutiérrez, Alberto Alape-Girón, Jan Felix Drexler

Source: Frontiers in Medicine, Vol 8 (2021)

Subject Terms: equine antibodies, SARS-CoV-2, therapy, variant of concern, PRNT titers 50, neutralization test, Medicine (General), R5-920

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmed.2021.735853/full; https://doaj.org/toc/2296-858X

Access URL: https://doaj.org/article/5c7bd487a62f49e99fe55294c82b87f6

Antibody Response to SARS-CoV-2 among COVID-19 Confirmed Cases and Correlates with Neutralizing Assay in a Subgroup of Patients in Delhi National Capital Region, India

Authors: Puneet Misra, Shashi Kant, Randeep Guleria, Sanjay K. Rai, Abhishek Jaiswal, Suprakash Mandal, Guruprasad R. Medigeshi, Mohammad Ahmad, Anisur Rahman, Meenu Sangral, Kapil Yadav, Mohan Bairwa, Partha Haldar, Parveen Kumar

Source: Vaccines, Vol 10, Iss 8, p 1312 (2022)

Subject Terms: SARS-CoV-2, COVID-19, antibody, PRNT, neutralizing antibody, Medicine

File Description: electronic resource

Relation: https://www.mdpi.com/2076-393X/10/8/1312; https://doaj.org/toc/2076-393X

Access URL: https://doaj.org/article/51dee8087ad945e98e21eddce3acac25

Zika Virus Seropositivity in 1–4-Year-Old Children, Indonesia, 2014

Authors: R. Tedjo Sasmono, Rama Dhenni, Benediktus Yohan, Paul Pronyk, Sri Rezeki Hadinegoro, Elizabeth Jane Soepardi, Chairin Nisa Ma’roef, Hindra I. Satari, Heather Menzies, William A. Hawley, Ann M. Powers, Ronald Rosenberg, Khin Saw Aye Myint, Amin Soebandrio

Source: Emerging Infectious Diseases, Vol 24, Iss 9, Pp 1740-1743 (2018)

Subject Terms: serology, PRNT, Indonesia, Zika virus, viruses, vector-borne infections, Medicine, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: https://wwwnc.cdc.gov/eid/article/24/9/18-0582_article; https://doaj.org/toc/1080-6040; https://doaj.org/toc/1080-6059

Access URL: https://doaj.org/article/836cf737668d4e54b71b804bde6ef4bf