Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Authors: Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier Gerrit de Jong, Roel Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans

Source: Molecular Therapy: Nucleic Acids, Vol 25, Iss , Pp 696-707 (2021)

Subject Terms: CRISPR-Cas9, gene editing, T cells, stem cells, photoporation, intracellular delivery, Therapeutics. Pharmacology, RM1-950

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2162253121002080; https://doaj.org/toc/2162-2531

Access URL: https://doaj.org/article/d83a80530b204562bf8701e1aa7bfddd