Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

Authors: Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost

Source: eLife, Vol 11 (2022)

Subject Terms: CRISPR, CRISPR interference, genetic screen, functional genomics, knockdown, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/81856; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/af03cd98b4f34c128b98cd963fa2ba56

The AAA + ATPase TorsinA polymerizes into hollow helical tubes with 8.5 subunits per turn

Authors: F. Esra Demircioglu, Weili Zheng, Alexander J. McQuown, Nolan K. Maier, Nicki Watson, Iain M. Cheeseman, Vladimir Denic, Edward H. Egelman, Thomas U. Schwartz

Source: Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)

Subject Terms: Science

File Description: electronic resource

Relation: https://doaj.org/toc/2041-1723

Access URL: https://doaj.org/article/c1401ed5770944979beff084b9fa8646