Detection of Fasciola hepatica using nested-PCR in the slaughterhouses of Alborz, Iran

Authors: A. Rezaee Kalvani, L. Abdolmohammadi Khiav, S. R. Hosseini

Source: Bulgarian Journal of Veterinary Medicine, Vol 27, Iss 4, Pp 639-645 (2024)

Subject Terms: cattle, detection, epidemiology, fasciola hepatica, nested-pcr, sheep, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: https://doaj.org/toc/1311-1477; https://doaj.org/toc/1313-3543

Access URL: https://doaj.org/article/fdbd4f230d124c669270bb71213d375b

Efficacy of amniotic fluid, blood and urine samples for the diagnosis of toxoplasmosis in pregnant women candidates for amniocentesis using serological and molecular techniques

Authors: Rohallah Abedian, Bahman Rahimi Esboei, Shirafkan Kordi, Hadi Shokrollahnia Roshan, Hajar Ziaei Hezarjaribi, Zahra Rahmani, Mahbobeh Montazeri, Mahdi Fakhar

Source: BMC Pregnancy and Childbirth, Vol 24, Iss 1, Pp 1-10 (2024)

Subject Terms: Toxoplasma Gondii, Amniocentesis, Pregnant women, Nested PCR, Real-time PCR, Gynecology and obstetrics, RG1-991

File Description: electronic resource

Relation: https://doaj.org/toc/1471-2393

Access URL: https://doaj.org/article/3c0b64f0cb89440e83feab84d0b093fa

Study of rotavirus genotypes G and P in one Egyptian center-cross-sectional study

Authors: AbelRahman Eid Mahmoud, Maysaa El Sayed Zaki, Eman Hamdy Mohamed, Ehab M. Fahmy, Sanaa Samir Mohamed Hamam, Mona Abdellatif Alsayed

Source: Italian Journal of Pediatrics, Vol 50, Iss 1, Pp 1-8 (2024)

Subject Terms: Children, Rotavirus, Genotypes G, Genotypes P, Nested PCR, Pediatrics, RJ1-570

File Description: electronic resource

Relation: https://doaj.org/toc/1824-7288

Access URL: https://doaj.org/article/5d78fbce4aa0469e8c3fc0006ee9c59e

A rapid and sensitive extraction-free HiFi-LAMP assay for detecting Mycobacterium leprae

Authors: Zhengfang Wang, Xi Yang, Zhi-Ze Wang, Xiu-Feng Yu, Zhe Li, Shangwen Song, Yongjuan Zhao, Yi-Qun Kuang, Yu-Ye Li, Chiyu Zhang

Source: International Journal of Infectious Diseases, Vol 152, Iss , Pp 107835- (2025)

Subject Terms: M. leprae, HiFi-LAMP, Extraction-free, Saliva, Point-of-care testing (POCT), Nested PCR, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S120197122500058X; https://doaj.org/toc/1201-9712

Access URL: https://doaj.org/article/b8f04712d55d4e44a1003a391b3c249b

Prevalence of Toxoplasma gondii in retail venison in Montreal detected using a nested PCR method

Authors: Chi Zhao, Mengyu Zhang, Jiaxuan Lan, Xiaoqi Mi, Xiaonan Lu, Qian Liu

Source: Journal of Agriculture and Food Research, Vol 19, Iss , Pp 101691- (2025)

Subject Terms: Toxoplasma gondii, Venison, Prevalence, Nested PCR, RE-529 gene, Foodborne parasite, Agriculture (General), S1-972, Nutrition. Foods and food supply, TX341-641

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2666154325000626; https://doaj.org/toc/2666-1543

Access URL: https://doaj.org/article/d9038b5d04464bb0bf32bb2193cf0ab8

Molecular Detection and Phylogenetic Analysis of Orf Virus From Dermatological Lesions in the Teats of Goats

Authors: Yakup Yıldırım, Seval Bilge Dağalp, Gökhan Bozkurt, Fırat Doğan, Ali Küçük

Source: Veterinary Medicine and Science, Vol 11, Iss 1, Pp n/a-n/a (2025)

Subject Terms: goat, orf virus, phylogenetic analyses, semi‐nested PCR, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: https://doaj.org/toc/2053-1095

Access URL: https://doaj.org/article/0086d67b030f4bb38435b7cbefa0700b

Equine piroplasmosis in different geographical areas in France: Prevalence heterogeneity of asymptomatic carriers and low genetic diversity of Theileria equi and Babesia caballi

Authors: Maggy Jouglin, Claire Bonsergent, Nathalie de la Cotte, Mickaël Mège, Céline Bizon, Anne Couroucé, Élodie-Anne Lallemand, Agnès Leblond, Louise C Lemonnier, Aurélia Leroux, Ilaria Marano, Alexandre Muzard, Émilie Quéré, Marion Toussaint, Albert Agoulon, Laurence Malandrin

Source: Ticks and Tick-Borne Diseases, Vol 16, Iss 1, Pp 102434- (2025)

Subject Terms: Equine piroplasmosis, Asymptomatic carriers, Nested PCR, Theileria equi, Babesia caballi, Genotype, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S1877959X24001274; https://doaj.org/toc/1877-9603

Access URL: https://doaj.org/article/2b1e713d66da4a7294adac4243698935

Molecular Diagnosis and Typing of Cryptosporidium spp. Species in Human Stools with Diarrhea

Authors: Fatma Özkan, Anil İça

Source: Türkiye Parazitoloji Dergisi, Vol 48, Iss 3, Pp 184-190 (2024)

Subject Terms: 18s rrna, cryptosporidium, nested pcr, pcr, sequencing, Medicine, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: https://doaj.org/toc/1300-6320; https://doaj.org/toc/2146-3077

Access URL: https://doaj.org/article/21eee485cd6544b0bc186ec68ecbc88b

Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi

Authors: Saipul Abbas, Ernawati Djaya, Erwin Najamuddin, Amelia Sebayang, Ayyub Ar Rahman, Aminah Aminah, Hasbi Hasbi, Surianto Sipi, Nur Fathurahman Ridwan, Rini Ismayanti, Elisurya Ibrahim

Source: Jurnal Perlindungan Tanaman Indonesia, Vol 28, Iss 1, Pp 33-45 (2024)

Subject Terms: aster yellows phytoplasma, nested pcr, sogatella vibix, rice, sulawesi, Agriculture (General), S1-972, Plant culture, SB1-1110

File Description: electronic resource

Relation: https://jurnal.ugm.ac.id/jpti/article/view/84736; https://doaj.org/toc/1410-1637; https://doaj.org/toc/2548-4788

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Epidemiological Features of Leptospirosis and Identification of Leptospira wolffii as a Persistently Prevailing Species in North–Central Bangladesh

Authors: Monira Sultana, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Nazia Haque, Md. Kamrul Hasan, Arup Islam, Sultana Shabnam Nila, Afsana Jahan, Fardousi Akter Sathi, Tasmia Hossain, Syeda Jannatul Ferdaus, Meiji Soe Aung, Nobumichi Kobayashi

Source: Infectious Disease Reports, Vol 16, Iss 4, Pp 638-649 (2024)

Subject Terms: leptospirosis, IgM LAT, IgM ELISA, nested PCR, Leptospira wolffii, Bangladesh, Other systems of medicine, RZ201-999

File Description: electronic resource

Relation: https://www.mdpi.com/2036-7449/16/4/49; https://doaj.org/toc/2036-7449

Access URL: https://doaj.org/article/09f3d574ed6c421589007b84c01c6b94

Serological and molecular detection of Toxoplasma gondii in chickens in southern Syria

Authors: Dhifaf Mahmood, Samar Al Nahhas

Source: Food and Waterborne Parasitology, Vol 39, Iss , Pp e00255- (2025)

Subject Terms: Toxoplasma gondii, Chicken, Indirect hemagglutination, Semi-nested PCR, Syria, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2405676625000022; https://doaj.org/toc/2405-6766

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First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan

Authors: Khalid Salman, Sittna Hayder El Nagar, Abdel Rahim M. El Hussein, Mohammed A. El Hussein, Hadi M. Yassine, Hebah A. Al Khatib, Mashael Ali Al- Badr, Ibrahim Farah, Khalid A. Enan

Source: Journal of Infection and Public Health, Vol 18, Iss 4, Pp 102682- (2025)

Subject Terms: Sickle cell anaemia, Parvovirus B19, Seroprevalence, Genotypes, Nested PCR, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S1876034125000310; https://doaj.org/toc/1876-0341

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Human papillomavirus as a potential etiological factor and biomarker in bladder carcinogenesis: A molecular epidemiological study

Authors: Aqerrout, Mouna ^a, Laraqui, Abdelilah ^b, Ennibi, Khalid ^c, Ameur, Ahmed ^d, Hamedoun, Larbi ^d, Alami, Mohammed ^d, El Ghazzaly, Anouar ^e, Ennaji, Moulay Mustapha ^{a, ⁎}

Source: In Gene Reports March 2025 38

Characterization of phytoplasma associated with wilt disease in coconut and approaches for its sensitive diagnostics

Authors: Boopathi, Natesan ^a, Karthikeyan, Gandhi ^{a, ⁎}, Raveendran, Muthurajan ^b, Johnson, Iruthayasamy ^a, Maruthasalam, Subbaiyan ^a, Srinivasan, Thulasy ^c, Manimekalai, Ramaswamy ^d

Source: In Journal of Microbiological Methods January 2025 228

Molecular detection and phylogeny analysis of Coxiella burnetii detected from cattle and buffalo milk based on plasmid cbhE gene in West Azerbaijan of Iran

Authors: Peyman Khademi, Amir Tukmechi, Abdulghaffar Ownagh

Source: New Microbes and New Infections, Vol 62, Iss , Pp 101495- (2024)

Subject Terms: Buffalo, Cattle, Coxiella burnetii, Milk, Nested-PCR, Plasmid, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2052297524002798; https://doaj.org/toc/2052-2975

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Cryptosporidium species and subtypes in Norway: predominance of C. parvum and emergence of C. mortiferum

Authors: Jahid Hasan Tipu, Audun Sivertsen, Jan-Egil Afset, Lars Sandven, Hanne Brekke, Hilde Marie Lund, Linnea Sofie Elburg, Peter Gaustad, Tore Lier, Liv Reidun Tverelv, Øystein Haarklau Johansen, Lucy J. Robertson, Kurt Hanevik

Source: Emerging Microbes and Infections, Vol 13, Iss 1 (2024)

Subject Terms: Cryptosporidiosis, molecular epidemiology, imported, seasonality, regionality, nested PCR, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

File Description: electronic resource

Relation: https://doaj.org/toc/2222-1751

Access URL: https://doaj.org/article/1b2862c82324450ab00592dab6abbb83

eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers

Authors: Bo‐Young Lee, Grant A. Milne, Corwin Freedman, Jenifer Miksis‐Olds, Bonnie L. Brown

Source: Environmental DNA, Vol 6, Iss 6, Pp n/a-n/a (2024)

Subject Terms: 12S rRNA, bony fish, eDNA metabarcoding, Gulf of Maine, nested PCR, Environmental sciences, GE1-350, Microbial ecology, QR100-130

File Description: electronic resource

Relation: https://doaj.org/toc/2637-4943

Access URL: https://doaj.org/article/703fcb7d14e648e6abfb83f39ccde257

Microscopic and molecular detection of Trichomonas vaginalis in outpatients seeking medical care in Upper Egypt

Authors: Nasser Mohamed Abd El-kareem, Ahmed Kamal Dyab, Nada Oudah Albalawi, Abdalla Abd El Samea, Mohamed Ahmed Ali Taha, Hajar AlQadeeb, Ahmed Gareh, Elham Adel Hiekal, Hind Alzaylaee, Ehab Kotb Elmahallawy

Source: Frontiers in Microbiology, Vol 15 (2024)

Subject Terms: Trichomonas vaginalis, nested PCR, genotyping, RFLP, Upper Egypt, Microbiology, QR1-502

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fmicb.2024.1499270/full; https://doaj.org/toc/1664-302X

Access URL: https://doaj.org/article/ca52d0b1303448918a67684d9ab5683a

Cryptosporidium spp. in German wildlife: Detection, regional occurrence and diversity in wild boar, roe, red and fallow deer

Authors: Claudia Jäckel, Iryna Hrushetska, Anne Mayer-Scholl, Jens A. Hammerl, Annette Johne, Carl Gremse, Denny Maaz, Karsten Nöckler, Martin Heinrich Richter

Source: Heliyon, Vol 10, Iss 21, Pp e38548- (2024)

Subject Terms: Sample preparation, Nested-PCR, Prevalence, Detection, Game, Cryptosporidium, Science (General), Q1-390, Social sciences (General), H1-99

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2405844024145793; https://doaj.org/toc/2405-8440

Access URL: https://doaj.org/article/e2fa684455324164a111dd6e68b780ef

Uracil walking primer PCR: An accurate and efficient genome-walking tool

Authors: Chen, Hong ^{a, b, d}, Tian, Bingkun ^{a, b, d}, Wang, Rongrong ^{a, b, d}, Pan, Zhenkang ^{a, d}, Gao, Dandan ^{c, ⁎}, Li, Haixing ^{a, b, d, ⁎}

Source: In Journal of Genetic Engineering and Biotechnology June 2025 23(2)