Highly efficient in vivo hematopoietic stem cell transduction using an optimized self-complementary adeno-associated virus

Authors: Charlesworth, Carsten T. ¹, Homma, Shota ^{1, 2}, Amaya, Anais K. ³, Dib, Carla ^{1, 3}, Vaidyanathan, Sriram ⁷, Tan, Tze-Kai ^{1, 5}, Miyauchi, Masashi ^{1, 2}, Nakauchi, Yusuke ^{1, 4}, Suchy, Fabian P. ^{1, 2}, Wang, Sicong ¹, Igarashi, Kyomi J. ^{1, 2}, Cromer, M. Kyle ⁶, Dudek, Amanda M. ^{1, 3}, Amorin, Alvaro ^{1, 3}, Czechowicz, Agnieszka ^{1, 3, ∗}, Wilkinson, Adam C. ^{8, ∗∗}, Nakauchi, Hiromitsu ^{1, 2, 9, ∗∗∗}

Source: In Molecular Therapy - Methods & Clinical Development 13 March 2025 33(1)

Generation of insulin-like growth factor 1 receptor-knockout pigs as a potential system for interspecies organogenesis

Authors: Nagaya, Masaki, Uchikura, Ayuko, Nakano, Kazuaki, Watanabe, Masahito, Matsunari, Hitomi, Umeyama, Kazuhiro, Mizuno, Naoaki, Nishimura, Toshiya, Nakauchi, Hiromitsu, Nagashima, Hiroshi

Source: In Regenerative Therapy June 2024 26:783-791

Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells

Authors: Fowler, Jonas L., Zheng, Sherry Li, Nguyen, Alana, Chen, Angela, Xiong, Xiaochen, Chai, Timothy, Chen, Julie Y., Karigane, Daiki, Banuelos, Allison M., Niizuma, Kouta, Kayamori, Kensuke, Nishimura, Toshinobu, Cromer, M. Kyle, Gonzalez-Perez, David, Mason, Charlotte, Liu, Daniel Dan, Yilmaz, Leyla, Miquerol, Lucile, Porteus, Matthew H., Luca, Vincent C., Majeti, Ravindra, Nakauchi, Hiromitsu, Red-Horse, Kristy, Weissman, Irving L., Ang, Lay Teng, Loh, Kyle M.

Source: In Developmental Cell 6 May 2024 59(9):1110-1131

Physioxia improves the selectivity of hematopoietic stem cell expansion cultures

Authors: Igarashi, Kyomi J., Kucinski, Iwo, Chan, Yan Yi, Tan, Tze-Kai, Khoo, Hwei Minn, Kealy, David, Bhadury, Joydeep, Hsu, Ian, Ho, Pui Yan, Niizuma, Kouta, Hickey, John W., Nolan, Garry P., Bridge, Katherine S., Czechowicz, Agnieszka, Gottgens, Berthold, Nakauchi, Hiromitsu, Wilkinson, Adam C.

Source: In Blood Advances 25 July 2023 7(14):3366-3377

Mechanical guidance of self-condensation patterns of differentiating progeny

Authors: Matsuzaki, Takahisa, Shimokawa, Yuko, Koike, Hiroyuki, Kimura, Masaki, Kawano, Yuma, Okuma, Nao, Kawamura, Ryuzo, Yoneyama, Yosuke, Furuichi, Yasuro, Hakuno, Fumihiko, Takahashi, Shin-Ichiro, Nakabayashi, Seiichiro, Okamoto, Satoshi, Nakauchi, Hiromitsu, Taniguchi, Hideki, Takebe, Takanori, Yoshikawa, Hiroshi Y.

Source: In iScience 21 October 2022 25(10)

Dual-antigen targeted iPSC-derived chimeric antigen receptor-T cell therapy for refractory lymphoma

Authors: Harada, Sakiko, Ando, Miki, Ando, Jun, Ishii, Midori, Yamaguchi, Tomoyuki, Yamazaki, Satoshi, Toyota, Tokuko, Ohara, Kazuo, Ohtaka, Manami, Nakanishi, Mahito, Shin, Chansu, Ota, Yasunori, Nakashima, Kazutaka, Ohshima, Koichi, Imai, Chihaya, Nakazawa, Yozo, Nakauchi, Hiromitsu, Komatsu, Norio

Source: Molecular therapy. 30(2):534-549

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100155641995.0x000001&indx=1&recIds=ETOCvdc_100155641995.0x000001

Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo

Authors: Nishimura, Toshinobu, Niizuma, Kouta, Nakauchi, Hiromitsu

Source: Methods in molecular biology. 2524:291-298

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN629813078&indx=1&recIds=ETOCRN629813078

Generation of Tfap2c‐T2A‐tdTomato knock‐in reporter rats via adeno‐associated virus‐mediated efficient gene targeting

Authors: Oikawa, Mami, Nagae, Mayuko, Mizuno, Naoaki, Iwatsuki, Kenyu, Yoshida, Fumika, Inoue, Naoko, Uenoyama, Yoshihisa, Tsukamura, Hiroko, Nakauchi, Hiromitsu, Hirabayashi, Masumi, Kobayashi, Toshihiro

Source: Molecular reproduction and development. 89(3):129-132

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100145919710.0x000001&indx=1&recIds=ETOCvdc_100145919710.0x000001

Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Authors: Roodgar, Morteza ^{1, 2, ∗}, Suchy, Fabian P. ², Nguyen, Lan H. ³, Bajpai, Vivek K. ^{2, 4}, Sinha, Rahul ², Vilches-Moure, Jose G. ⁵, Van Bortle, Kevin ¹, Bhadury, Joydeep ^{2, 10}, Metwally, Ahmed ¹, Jiang, Lihua ¹, Jian, Ruiqi ¹, Chiang, Rosaria ¹, Oikonomopoulos, Angelos ⁶, Wu, Joseph C. ⁶, Weissman, Irving L. ², Mankowski, Joseph L. ⁷, Holmes, Susan ⁸, Loh, Kyle M. ^{2, 11}, Nakauchi, Hiromitsu ^{1, 2, ∗∗}, VandeVoort, Catherine A. ^{9, ∗∗∗}, Snyder, Michael P. ^{1, 12, ∗∗∗∗}

Source: In Cell Reports 30 August 2022 40(9)

Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses

Authors: Ang, Lay Teng, Nguyen, Alana T., Liu, Kevin J., Chen, Angela, Xiong, Xiaochen, Curtis, Matthew, Martin, Renata M., Raftry, Brian C., Ng, Chun Yi, Vogel, Uwe, Lander, Angelika, Lesch, Benjamin J., Fowler, Jonas L., Holman, Alyssa R., Chai, Timothy, Vijayakumar, Siva, Suchy, Fabian P., Nishimura, Toshinobu, Bhadury, Joydeep, Porteus, Matthew H., Nakauchi, Hiromitsu, Cheung, Christine, George, Steven C., Red-Horse, Kristy, Prescott, Joseph B., Loh, Kyle M.

Source: In Cell 7 July 2022 185(14):2523-2541

Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors.

Authors: Suchy, Fabian P., Karigane, Daiki, Nakauchi, Yusuke, Higuchi, Maimi, Zhang, Jinyu, Pekrun, Katja, Hsu, Ian, Fan, Amy C., Nishimura, Toshinobu, Charlesworth, Carsten T., Bhadury, Joydeep, Nishimura, Toshiya, Wilkinson, Adam C., Kay, Mark A., Majeti, Ravindra, Nakauchi, Hiromitsu

Source: Nature Biotechnology; Feb2025, Vol. 43 Issue 2, p204-213, 10p

Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation

Authors: Watanabe, Masahito, Umeyama, Kazuhiro, Nakano, Kazuaki, Matsunari, Hitomi, Fukuda, Toru, Matsumoto, Kei, Tajiri, Susumu, Yamanaka, Shuichiro, Hasegawa, Koki, Okamoto, Kazutoshi, Uchikura, Ayuko, Takayanagi, Shuko, Nagaya, Masaki, Yokoo, Takashi, Nakauchi, Hiromitsu, Nagashima, Hiroshi

Source: In Laboratory Investigation May 2022 102(5):560-569

Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells

Authors: Kobayashi, Toshihiro, Castillo-Venzor, Aracely, Penfold, Chris A., Morgan, Michael, Mizuno, Naoaki, Tang, Walfred W.C., Osada, Yasuyuki, Hirao, Masao, Yoshida, Fumika, Sato, Hideyuki, Nakauchi, Hiromitsu, Hirabayashi, Masumi, Surani, M. Azim

Source: In Cell Reports 12 October 2021 37(2)

High glucose macrophage exosomes enhance atherosclerosis by driving cellular proliferation & hematopoiesis

Authors: Bouchareychas, Laura, Duong, Phat, Phu, Tuan Anh, Alsop, Eric, Meechoovet, Bessie, Reiman, Rebecca, Ng, Martin, Yamamoto, Ryo, Nakauchi, Hiromitsu, Gasper, Warren J., Van Keuren-Jensen, Kendall, Raffai, Robert L.

Source: In iScience 20 August 2021 24(8)

ISSCR Guidelines for Stem Cell Research and Clinical Translation: The 2021 update

Authors: Lovell-Badge, Robin, Anthony, Eric, Barker, Roger A., Bubela, Tania, Brivanlou, Ali H., Carpenter, Melissa, Charo, R. Alta, Clark, Amander, Clayton, Ellen, Cong, Yali, Daley, George Q., Fu, Jianping, Fujita, Misao, Greenfield, Andy, Goldman, Steve A., Hill, Lori, Hyun, Insoo, Isasi, Rosario, Kahn, Jeffrey, Kato, Kazuto, Kim, Jin-Soo, Kimmelman, Jonathan, Knoblich, Jürgen A., Mathews, Debra, Montserrat, Nuria, Mosher, Jack, Munsie, Megan, Nakauchi, Hiromitsu, Naldini, Luigi, Naughton, Gail, Niakan, Kathy, Ogbogu, Ubaka, Pedersen, Roger, Rivron, Nicolas, Rooke, Heather, Rossant, Janet, Round, Jeff, Saitou, Mitinori, Sipp, Douglas, Steffann, Julie, Sugarman, Jeremy, Surani, Azim, Takahashi, Jun, Tang, Fuchou, Turner, Leigh, Zettler, Patricia J., Zhai, Xiaomei

Source: In Stem Cell Reports 8 June 2021 16(6):1398-1408

ISSCR guidelines for the transfer of human pluripotent stem cells and their direct derivatives into animal hosts

Authors: Hyun, Insoo, Clayton, Ellen Wright, Cong, Yali, Fujita, Misao, Goldman, Steven A., Hill, Lori R., Monserrat, Nuria, Nakauchi, Hiromitsu, Pedersen, Roger A., Rooke, Heather M., Takahashi, Jun, Knoblich, Jürgen A.

Source: In Stem Cell Reports 8 June 2021 16(6):1409-1415

In vivo clonal analysis of aging hematopoietic stem cells

Authors: Yamamoto, Ryo, Nakauchi, Hiromitsu ^*

Source: In Mechanisms of Ageing and Development December 2020 192

Sustainable Tumor-Suppressive Effect of iPSC-Derived Rejuvenated T Cells Targeting Cervical Cancers

Authors: Honda, Tadahiro, Ando, Miki, Ando, Jun, Ishii, Midori, Sakiyama, Yumi, Ohara, Kazuo, Toyota, Tokuko, Ohtaka, Manami, Masuda, Ayako, Terao, Yasuhisa, Nakanishi, Mahito, Nakauchi, Hiromitsu, Komatsu, Norio

Source: In Molecular Therapy 4 November 2020 28(11):2394-2405

Stabilizing hematopoietic stem cells in vitro

Authors: Wilkinson, Adam C, Nakauchi, Hiromitsu

Source: In Current Opinion in Genetics & Development October 2020 64:1-5

Hedgehog Activation Regulates Human Osteoblastogenesis

Authors: Onodera, Shoko, Saito, Akiko, Hojo, Hironori, Nakamura, Takashi, Zujur, Denise, Watanabe, Katsuhito, Morita, Nana, Hasegawa, Daigo, Masaki, Hideki, Nakauchi, Hiromitsu, Nomura, Takeshi, Shibahara, Takahiko, Yamaguchi, Akira, Chung, Ung-il, Azuma, Toshifumi, Ohba, Shinsuke

Source: In Stem Cell Reports 14 July 2020 15(1):125-139