Into the multiverse: advances in single-cell multiomic profiling

Authors: Ogbeide, Silvia, Giannese, Francesca, Mincarelli, Laura, Macaulay, Iain C.

Source: In Trends in Genetics August 2022 38(8):831-843

Transcriptional characterization of human megakaryocyte polyploidization and lineage commitment

Authors: Choudry, Fizzah A., Bagger, Frederik O. ^*, Macaulay, Iain C., Farrow, Samantha, Burden, Frances, Kempster, Carly, McKinney, Harriet, Olsen, Lars R., Huang, Ni, Downes, Kate, Voet, Thierry, Uppal, Rakesh, Martin, John F., Mathur, Anthony, Ouwehand, Willem H., Laurenti, Elisa, Teichmann, Sarah A., Frontini, Mattia

Source: In Journal of Thrombosis and Haemostasis May 2021 19(5):1236-1249

Low protein diet protects the liver from Salmonella Typhimurium-mediated injury by modulating the mTOR/autophagy axis in macrophages.

Authors: Wojtowicz, Edyta E., Hampton, Katherine, Moreno-Gonzalez, Mar, Utting, Charlotte L., Lan, Yuxuan, Ruiz, Paula, Beasy, Gemma, Bone, Caitlin, Hellmich, Charlotte, Maynard, Rebecca, Acton, Luke, Markham, Matthew, Troeberg, Linda, Telatin, Andrea, Kingsley, Robert A., Macaulay, Iain C., Rushworth, Stuart A., Beraza, Naiara

Source: Communications Biology; 9/30/2024, Vol. 7 Issue 1, p1-13, 13p

Subject Terms: SALMONELLA enterica serovar typhimurium, LOW-protein diet, LIVER cells, MYELOID cells, WESTERN diet

Identification of a non-canonical ciliate nuclear genetic code where UAA and UAG code for different amino acids.

Authors: McGowan, Jamie¹, Kilias, Estelle S.², Alacid, Elisabet², Lipscombe, James¹, Jenkins, Benjamin H.², Gharbi, Karim¹, Kaithakottil, Gemy G.¹, Macaulay, Iain C.¹, McTaggart, Seanna¹, Warring, Sally D.¹, Richards, Thomas A.², thomas.richards@biology.ox.ac.uk, Hall, Neil^1,3, thomas.richards@biology.ox.ac.uk, Swarbreck, David¹, thomas.richards@biology.ox.ac.uk

Source: PLoS Genetics; 10/5/2023, Vol. 19 Issue 10, p1-21, 21p

Deep sequencing of Phox2a nuclei reveals five classes of anterolateral system neurons.

Authors: Bell, Andrew M., Utting, Charlotte, Dickie, Allen C., Kucharczyk, Mateusz W., Quillet, Raphaëlle, Gutierrez-Mecinas, Maria, Razlan, Aimi N. B., Cooper, Andrew H., Yuxuan Lan, Hachisuka, Junichi, Weir, Greg A., Bannister, Kirsty, Masahiko Watanabe, Kania, Artur, Hoon, Mark A., Macaulay, Iain C., Denk, Franziska, Todd, Andrew J.

Source: Proceedings of the National Academy of Sciences of the United States of America; 6/4/2024, Vol. 121 Issue 23, p1-32, 43p

Subject Terms: NEURONS, SPINAL cord, SKIN temperature, IN situ hybridization, PAIN perception

Single-Cell Multiomics: Multiple Measurements from Single Cells

Authors: Macaulay, Iain C., Ponting, Chris P., Voet, Thierry

Source: In Trends in Genetics February 2017 33(2):155-168

Heterogeneity in Oct4 and Sox2 Targets Biases Cell Fate in 4-Cell Mouse Embryos

Authors: Goolam, Mubeen, Scialdone, Antonio, Graham, Sarah J.L., Macaulay, Iain C., Jedrusik, Agnieszka, Hupalowska, Anna, Voet, Thierry, Marioni, John C., Zernicka-Goetz, Magdalena

Source: In Cell 24 March 2016 165(1):61-74

Single-Cell RNA-Sequencing Reveals a Continuous Spectrum of Differentiation in Hematopoietic Cells

Authors: Macaulay, Iain C., Svensson, Valentine, Labalette, Charlotte, Ferreira, Lauren, Hamey, Fiona, Voet, Thierry, Teichmann, Sarah A., Cvejic, Ana

Source: In Cell Reports 2 February 2016 14(4):966-977

Combined Single-Cell Functional and Gene Expression Analysis Resolves Heterogeneity within Stem Cell Populations

Authors: Wilson, Nicola K., Kent, David G., Buettner, Florian, Shehata, Mona, Macaulay, Iain C., Calero-Nieto, Fernando J., Sánchez Castillo, Manuel, Oedekoven, Caroline A., Diamanti, Evangelia, Schulte, Reiner, Ponting, Chris P., Voet, Thierry, Caldas, Carlos, Stingl, John, Green, Anthony R., Theis, Fabian J., Göttgens, Berthold

Source: In Cell Stem Cell 4 June 2015 16(6):712-724

Proliferation Drives Aging-Related Functional Decline in a Subpopulation of the Hematopoietic Stem Cell Compartment

Authors: Kirschner, Kristina, Chandra, Tamir, Kiselev, Vladimir, Flores-Santa Cruz, David, Macaulay, Iain C., Park, Hyun Jun, Li, Juan, Kent, David G., Kumar, Rupa, Pask, Dean C., Hamilton, Tina L., Hemberg, Martin, Reik, Wolf, Green, Anthony R.

Source: Cell reports. 19(8):1503-1511

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100063136279.0x000001&indx=1&recIds=ETOCvdc_100063136279.0x000001

Single-cell transcriptome analysis of fish immune cells provides insight into the evolution of vertebrate immune cell types

Authors: Carmona, Santiago J., Teichmann, Sarah A., Ferreira, Lauren, Macaulay, Iain C., Stubbington, Michael J. T., Cvejic, Ana, Gfeller, David

Source: Genome research. 27(3):451-461

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN610306339&indx=1&recIds=ETOCRN610306339

Myelodysplastic Syndromes Are Propagated by Rare and Distinct Human Cancer Stem Cells In Vivo

Authors: Woll, Petter S., Kjällquist, Una, Chowdhury, Onima, Doolittle, Helen, Wedge, David C., Thongjuea, Supat, Erlandsson, Rikard, Ngara, Mtakai, Anderson, Kristina, Deng, Qiaolin, Mead, Adam J., Stenson, Laura, Giustacchini, Alice, Duarte, Sara, Giannoulatou, Eleni, Taylor, Stephen, Karimi, Mohsen, Scharenberg, Christian, Mortera-Blanco, Teresa, Macaulay, Iain C., Clark, Sally-Ann, Dybedal, Ingunn, Josefsen, Dag, Fenaux, Pierre, Hokland, Peter, Holm, Mette S., Cazzola, Mario, Malcovati, Luca, Tauro, Sudhir, Bowen, David, Boultwood, Jacqueline, Pellagatti, Andrea, Pimanda, John E., Unnikrishnan, Ashwin, Vyas, Paresh, Göhring, Gudrun, Schlegelberger, Brigitte, Tobiasson, Magnus, Kvalheim, Gunnar, Constantinescu, Stefan N., Nerlov, Claus, Nilsson, Lars, Campbell, Peter J., Sandberg, Rickard, Papaemmanuil, Elli, Hellström-Lindberg, Eva, Linnarsson, Sten, Jacobsen, Sten Eirik W.

Source: In Cancer Cell 16 June 2014 25(6):794-808

Transcriptional diversity during lineage commitment of human blood progenitors

Authors: Chen, Lu, Kostadima, Myrto, Martens, Joost H. A., Canu, Giovanni, Garcia, Sara P., Turro, Ernest, Downes, Kate, Macaulay, Iain C., Bielczyk-Maczynska, Ewa, Coe, Sophia, Farrow, Samantha, Poudel, Pawan, Burden, Frances, Jansen, Sjoert B. G., Astle, William J., Attwood, Antony, Bariana, Tadbir, de Bono, Bernard, Breschi, Alessandra, Chambers, John C., Consortium, BRIDGE, Choudry, Fizzah A., Clarke, Laura, Coupland, Paul, van der Ent, Martijn, Erber, Wendy N., Jansen, Joop H., Favier, Rémi, Fenech, Matthew E., Foad, Nicola, Freson, Kathleen, van Geet, Chris, Gomez, Keith, Guigo, Roderic, Hampshire, Daniel, Kelly, Anne M., Kerstens, Hindrik H. D., Kooner, Jaspal S., Laffan, Michael, Lentaigne, Claire, Labalette, Charlotte, Martin, Tiphaine, Meacham, Stuart, Mumford, Andrew, Nürnberg, Sylvia, Palumbo, Emilio, van der Reijden, Bert A., Richardson, David, Sammut, Stephen J., Slodkowicz, Greg, Tamuri, Asif U., Vasquez, Louella, Voss, Katrin, Watt, Stephen, Westbury, Sarah, Flicek, Paul, Loos, Remco, Goldman, Nick, Bertone, Paul, Read, Randy J., Richardson, Sylvia, Cvejic, Ana, Soranzo, Nicole, Ouwehand, Willem H., Stunnenberg, Hendrik G., Frontini, Mattia, Rendon, Augusto

Source: Science, 2014 Sep . 345(6204), 1580-1580.

Access URL: https://www.jstor.org/stable/24917692

Lymphomyeloid Contribution of an Immune-Restricted Progenitor Emerging Prior to Definitive Hematopoietic Stem Cells

Authors: Böiers, Charlotta, Carrelha, Joana, Lutteropp, Michael, Luc, Sidinh, Green, Joanna C.A., Azzoni, Emanuele, Woll, Petter S., Mead, Adam J., Hultquist, Anne, Swiers, Gemma, Perdiguero, Elisa Gomez, Macaulay, Iain C., Melchiori, Luca, Luis, Tiago C., Kharazi, Shabnam, Bouriez-Jones, Tiphaine, Deng, Qiaolin, Pontén, Annica, Atkinson, Deborah, Jensen, Christina T., Sitnicka, Ewa, Geissmann, Frederic, Godin, Isabelle, Sandberg, Rickard, de Bruijn, Marella F.T.R., Jacobsen, Sten Eirik W.

Source: In Cell Stem Cell 7 November 2013 13(5):535-548

Canonical Wnt signaling in megakaryocytes regulates proplatelet formation

Authors: Macaulay, Iain C. ^*, Thon, Jonathan N. ^*, Tijssen, Marloes R., Steele, Brian M., MacDonald, Bryan T., Meade, Gerardene, Burns, Philippa, Rendon, Augusto, Salunkhe, Vishal, Murphy, Ronan P., Bennett, Cavan, Watkins, Nicholas A., He, Xi, Fitzgerald, Desmond J., Italiano, Joseph E., Jr, Maguire, Patricia B. ^*

Source: In Blood 3 January 2013 121(1):188-196

Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner.

Authors: Luis, Tiago C., Barkas, Nikolaos, Carrelha, Joana, Giustacchini, Alice, Mazzi, Stefania, Norfo, Ruggiero, Wu, Bishan, Aliouat, Affaf, Guerrero, Jose A., Rodriguez-Meira, Alba, Bouriez-Jones, Tiphaine, Macaulay, Iain C., Jasztal, Maria, Zhu, Guangheng, Ni, Heyu, Robson, Matthew J., Blakely, Randy D., Mead, Adam J., Nerlov, Claus, Ghevaert, Cedric

Source: Nature Communications; 9/28/2023, Vol. 14 Issue 1, p1-18, 18p

Subject Terms: HEMATOPOIETIC stem cells, HOMEOSTASIS, INTERLEUKIN-1, BLOOD cells, EXTRACELLULAR fluid, BLOOD platelet activation, BONE marrow, THROMBOPOIETIN receptors

Canonical Wnt Signaling Negatively Regulates Platelet Function

Authors: Steele, Brian M., Harper, Matthew T., Macaulay, Iain C., Morrell, Craig N., Perez-Tamayo, Alita, Foy, Martina, Habas, Raymond, Poole, Alastair W., Fitzgerald, Desmond J., Maguire, Patricia B.

Source: Proceedings of the National Academy of Sciences of the United States of America, 2009 Nov . 106(47), 19836-19841.

Access URL: https://www.jstor.org/stable/25593285

Panhematopoietic RNA barcoding enables kinetic measurements of nucleate and anucleate lineages and the activation of myeloid clones following acute platelet depletion.

Authors: Wojtowicz, Edyta E., Mistry, Jayna J., Uzun, Vladimir, Hellmich, Charlotte, Scoones, Anita, Chin, Desmond W., Kettyle, Laura M., Grasso, Francesca, Lord, Allegra M., Wright, David J., Etherington, Graham J., Woll, Petter S., Belderbos, Mirjam E., Bowles, Kristian M., Nerlov, Claus, Haerty, Wilfried, Bystrykh, Leonid V., Jacobsen, Sten Eirik W., Rushworth, Stuart A., Macaulay, Iain C.

Source: Genome Biology; 6/27/2023, Vol. 24 Issue 1, p1-27, 27p

Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells.

Authors: Mincarelli, Laura, Uzun, Vladimir, Wright, David, Scoones, Anita, Rushworth, Stuart A., Haerty, Wilfried, Macaulay, Iain C.

Source: Communications Biology; 5/24/2023, Vol. 6 Issue 1, p1-11, 11p

Subject Terms: HEMATOPOIETIC stem cells, GENE expression, ALTERNATIVE RNA splicing, CYTOKINE receptors, RNA splicing, STEM cells, TRANSCRIPTION factors

Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function

Authors: Goodall, Alison H. ^*, Burns, Philippa, Salles, Isabelle, Macaulay, Iain C., Jones, Chris I., Ardissino, Diego, de Bono, Bernard, Bray, Sarah L., Deckmyn, Hans, Dudbridge, Frank, Fitzgerald, Desmond J., Garner, Stephen F., Gusnanto, Arief, Koch, Kerstin, Langford, Cordelia, O'Connor, Marie N., Rice, Catherine M., Stemple, Derek, Stephens, Jonathan, Trip, Mieke D., Zwaginga, Jaap-Jan, Samani, Nilesh J., Watkins, Nicholas A., Maguire, Patricia B., Ouwehand, Willem H.

Source: In Blood 25 November 2010 116(22):4646-4656