Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts

Authors: Masanori Izumi, Sakuya Nakamura, Kohei Otomo, Hiroyuki Ishida, Jun Hidema, Tomomi Nemoto, Shinya Hagihara

Source: eLife, Vol 12 (2024)

Subject Terms: chloroplast, autophagy, plant, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/93232; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/2e0bf1204f9c4de687118106e47e8b6f

descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Authors: Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J. Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, Rin Yanai, Naruhiko Sahara, Satoshi Takagi, Ryohei Katayama, Yusuke Iwata, Toshiro Shiokawa, Yoku Hayakawa, Kensuke Otsuka, Haruko Watanabe-Takano, Yuka Haneda, Shigetomo Fukuhara, Miku Fujiwara, Takenobu Nii, Chikara Meno, Naoki Takeshita, Kenta Yashiro, Juan Marcelo Rosales Rocabado, Masaru Kaku, Tatsuya Yamada, Yumiko Oishi, Hiroyuki Koike, Yinglan Cheng, Keisuke Sekine, Jun-ichiro Koga, Kaori Sugiyama, Kenichi Kimura, Fuyuki Karube, Hyeree Kim, Ichiro Manabe, Tomomi Nemoto, Kazuki Tainaka, Akinobu Hamada, Hjalmar Brismar, Etsuo A. Susaki

Source: Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)

Subject Terms: Science

File Description: electronic resource

Relation: https://doaj.org/toc/2041-1723

Access URL: https://doaj.org/article/95dd009f5a8b43a5ace8ca6748af01ea

Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin

Authors: Taiga Takahashi, Hong Zhang, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto

Source: Communications Biology, Vol 7, Iss 1, Pp 1-13 (2024)

Subject Terms: Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/2399-3642

Access URL: https://doaj.org/article/b4d156fa52514f4697e3b16b9cffff18

Single-scan volumetric imaging throughout thick tissue specimens by one-touch installable light-needle creating device

Authors: Ching-Pu Chang, Kohei Otomo, Yuichi Kozawa, Hirokazu Ishii, Miwako Yamasaki, Masahiko Watanabe, Shunichi Sato, Ryosuke Enoki, Tomomi Nemoto

Source: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/3bbd637911584cbb81cd3e0381f50799

Improving two-photon excitation microscopy for sharper and faster biological imaging

Authors: Kohei Otomo, Hirokazu Ishii, Tomomi Nemoto

Source: Biophysics and Physicobiology, Vol 20 (2023)

Subject Terms: two-photon microscopy, adaptive optics, sted microscopy, multi-beam scanning, volumetric imaging, Biology (General), QH301-705.5, Physiology, QP1-981, Physics, QC1-999

File Description: electronic resource

Relation: https://doaj.org/toc/2189-4779

Access URL: https://doaj.org/article/7ac78847f7654d6da1b72667baa950fa

Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy

Authors: Takafumi Kamada, Kohei Otomo, Takashi Murata, Kaito Nakata, Shota Hiruma, Ryota Uehara, Mitsuyasu Hasebe, Tomomi Nemoto

Source: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/95cc33992d754286973b05863a7eb376

All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging

Authors: Hirokazu Ishii, Kohei Otomo, Ching-Pu Chang, Miwako Yamasaki, Masahiko Watanabe, Hiroyuki Yokoyama, Tomomi Nemoto

Source: PLoS ONE, Vol 18, Iss 8 (2023)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449175/?tool=EBI; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/09da5a380a4e4fb8b896448712352bb0

Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

Authors: Kazushi Yamaguchi, Kohei Otomo, Yuichi Kozawa, Motosuke Tsutsumi, Tomoko Inose, Kenji Hirai, Shunichi Sato, Tomomi Nemoto, Hiroshi Uji-i

Source: ACS Omega, Vol 6, Iss 1, Pp 438-447 (2020)

Subject Terms: Chemistry, QD1-999

File Description: electronic resource

Relation: https://doaj.org/toc/2470-1343

Access URL: https://doaj.org/article/41f6e676ff82486dbdb10b4ff07f75fe

Absorption, Fluorescence, and Two-Photon Excitation Ability of 5‑Phenylisolidolo[2,1‑a]quinolines

Authors: Ramon Francisco Avena, Lin Qiao, Yuki Fujii, Kohei Otomo, Hirokazu Ishii, Takeyuki Suzuki, Hirofumi Tsujino, Tadayuki Uno, Yasuo Tsutsumi, Yusuke Kawashima, Tatsuya Takagi, Kenichi Murai, Tomomi Nemoto, Mitsuhiro Arisawa

Source: ACS Omega, Vol 5, Iss 5, Pp 2473-2479 (2020)

Subject Terms: Chemistry, QD1-999

File Description: electronic resource

Relation: https://doaj.org/toc/2470-1343

Access URL: https://doaj.org/article/b2de29d239194afc887fc9cb1732eb20

Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain

Authors: Taiga Takahashi, Hong Zhang, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto

Source: STAR Protocols, Vol 2, Iss 2, Pp 100542- (2021)

Subject Terms: Microscopy, Neuroscience, Science (General), Q1-390

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2666166721002495; https://doaj.org/toc/2666-1667

Access URL: https://doaj.org/article/b90fa61665e54559823d3471f2c8c356

3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images

Authors: Chentao Wen, Takuya Miura, Venkatakaushik Voleti, Kazushi Yamaguchi, Motosuke Tsutsumi, Kei Yamamoto, Kohei Otomo, Yukako Fujie, Takayuki Teramoto, Takeshi Ishihara, Kazuhiro Aoki, Tomomi Nemoto, Elizabeth MC Hillman, Koutarou D Kimura

Source: eLife, Vol 10 (2021)

Subject Terms: cell tracking, bioimaging, deep learning, quantitative biology, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/59187; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/798a037c858b49c6b741110877257a1d

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

Authors: Taiga Takahashi, Hong Zhang, Ryosuke Kawakami, Kenji Yarinome, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto

Source: iScience, Vol 23, Iss 10, Pp 101579- (2020)

Subject Terms: Optical Imaging, Techniques in Neuroscience, Nanomaterials, Science

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2589004220307719; https://doaj.org/toc/2589-0042

Access URL: https://doaj.org/article/36d55eb4c78846e6adeb1eb806dac274

In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid.

Authors: Kazushi Yamaguchi, Ryoji Kitamura, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto

Source: PLoS ONE, Vol 15, Iss 8, p e0237230 (2020)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/31e88c9926ec4115b8aa5fcc65d41897

Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution.

Authors: Hong Zhang, Kenji Yarinome, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto, Yosuke Okamura

Source: PLoS ONE, Vol 15, Iss 1, p e0227650 (2020)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227650&type=printable; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/f0af36f59f0e4c5097a920cbbe933f1b

Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy

Authors: Ai Goto, Kohei Otomo, Tomomi Nemoto

Source: Frontiers in Physics, Vol 7 (2019)

Subject Terms: second harmonic generation, polarization-resolved optical microscopy, in vivo imaging, collagen, spinning-disk microscopy, two-photon excitation fluorescence microscopy, Physics, QC1-999

File Description: electronic resource

Relation: https://www.frontiersin.org/article/10.3389/fphy.2019.00056/full; https://doaj.org/toc/2296-424X

Access URL: https://doaj.org/article/d94dbeb99a804cd4afe59067bf693e07

Dynamics of a Fly Line (Effects of a Fly)

Authors: Kohei OTOMO, Tetsuya WATANABE, Akinori TOMODA

Source: Nihon Kikai Gakkai ronbunshu, Vol 81, Iss 827, Pp 14-00623-14-00623 (2015)

Subject Terms: fly, fly line, fly rod, leader, tippet, fly-casting, Mechanical engineering and machinery, TJ1-1570, Engineering machinery, tools, and implements, TA213-215

File Description: electronic resource

Relation: https://www.jstage.jst.go.jp/article/transjsme/81/827/81_14-00623/_pdf/-char/en; https://doaj.org/toc/2187-9761

Access URL: https://doaj.org/article/b768e673ccd14cf0ac0808ba66003a56

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STED microscopy--super-resolution bio-imaging utilizing a stimulated emission depletion.

Authors: Kohei Otomo¹, Terumasa Hibi^1,2, Yuichi Kozawa³, Tomomi Nemoto^1,2 tn@es.hokudai.ac.jp

Source: Microscopy. Aug2015, Vol. 64 Issue 4, p227-236. 10p.

Subject Terms: *IMAGING systems in biology, *MICROSCOPY, *HIGH resolution imaging, *OPTICAL scanners, *PHOTONS