Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression.

Authors: Natália G Sampaio, Martina Kocan, Louis Schofield, Kevin D G Pfleger, Emily M Eriksson

Source: PLoS ONE, Vol 13, Iss 8, p e0202408 (2018)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC6107161?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/a0e0618a92624bf9b0b0550edefe4b44

Functional interaction between angiotensin II receptor type 1 and chemokine (C-C motif) receptor 2 with implications for chronic kidney disease.

Authors: Mohammed Akli Ayoub, Yuan Zhang, Robyn S Kelly, Heng B See, Elizabeth K M Johnstone, Elizabeth A McCall, James H Williams, Darren J Kelly, Kevin D G Pfleger

Source: PLoS ONE, Vol 10, Iss 3, p e0119803 (2015)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC4373786?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/825dcccc2ee44c6ca84f5db253560016

Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signalling in a High-Throughput Format

Authors: Mohammed Akli eAyoub, Julien eTrebaux, Julie eVallaghe, Fabienne eCharrier-Savournin, Khaled eAl-Hosaini, Arturo eGonzalez-Moya, Jean-Philippe R Pin, Kevin D G Pfleger, Eric eTrinquet

Source: Frontiers in Endocrinology, Vol 5 (2014)

Subject Terms: GPCR, RTK, ERK1/2, EGFR., HTRF®, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

File Description: electronic resource

Relation: http://journal.frontiersin.org/Journal/10.3389/fendo.2014.00094/full; https://doaj.org/toc/1664-2392

Access URL: https://doaj.org/article/583f6d766fab406cb3c3a7bab1991e80

Biophysical detection of diversity and bias in GPCR function

Authors: Werner eJaeger, Stephen eArmstrong, Stephen eHill, Kevin D G Pfleger

Source: Frontiers in Endocrinology, Vol 5 (2014)

Subject Terms: Fluorescence Resonance Energy Transfer, BRET, bioluminescence resonance energy transfer, FRET, GPCR, GPCR-HIT, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

File Description: electronic resource

Relation: http://journal.frontiersin.org/Journal/10.3389/fendo.2014.00026/full; https://doaj.org/toc/1664-2392

Access URL: https://doaj.org/article/66419fb7d5af445889ae7876ec2c7553

Characterization of three vasopressin receptor 2 variants: an apparent polymorphism (V266A) and two loss-of-function mutations (R181C and M311V).

Authors: Stephen P Armstrong, Ruth M Seeber, Mohammed Akli Ayoub, Brian J Feldman, Kevin D G Pfleger

Source: PLoS ONE, Vol 8, Iss 6, p e65885 (2013)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC3675069?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/f326b9946bf546a8bc111b7d44bc2a91

Profiling epidermal growth factor receptor and heregulin receptor 3 heteromerization using receptor tyrosine kinase heteromer investigation technology.

Authors: Mohammed Akli Ayoub, Heng B See, Ruth M Seeber, Stephen P Armstrong, Kevin D G Pfleger

Source: PLoS ONE, Vol 8, Iss 5, p e64672 (2013)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC3659105?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/d870170f9b334f9a81b05246836f9afe

Deleterious GRM1 mutations in schizophrenia.

Authors: Mohammed Akli Ayoub, Dora Angelicheva, David Vile, David Chandler, Bharti Morar, Juleen A Cavanaugh, Peter M Visscher, Assen Jablensky, Kevin D G Pfleger, Luba Kalaydjieva

Source: PLoS ONE, Vol 7, Iss 3, p e32849 (2012)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22448230/?tool=EBI; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/bcb99bb2a31f46f09b9bce709f1ef34a

I8-arachnotocin–an arthropod-derived G protein-biased ligand of the human vasopressin V2 receptor

Authors: Leopold Duerrauer (Institute of Pharmacology, Center for Pharmacology and Physiology, Medical University of Vienna), Edin Muratspahić (Institute of Pharmacology, Center for Pharmacology and Physiology, Medical University of Vienna), Jasmin Gattringer (Institute of Pharmacology, Center for Pharmacology and Physiology, Medical University of Vienna), Peter Keov (School of Biomedical Sciences, Faculty of Medicine, The University of Queensland), Helen C. Mendel (Institute for Molecular Bioscience, The University of Queensland), Kevin D. G. Pfleger (Centre for Medical Research, The University of Western Australia and Harry Perkins Institute of Medical Research), Markus Muttenthaler (Department of Biological Chemistry, Faculty of Chemistry, University of Vienna), Christian W. Gruber (Institute of Pharmacology, Center for Pharmacology and Physiology, Medical University of Vienna)

Availability: http://data.europeana.eu/aggregation/europeana/1300/https___phaidra_univie_ac_at_o_1176403

GPCR heteromers: An overview of their classification, function and physiological relevance

Authors: Natasha C. Dale, Elizabeth K. M. Johnstone, Kevin D. G. Pfleger

Source: Frontiers in Endocrinology, Vol 13 (2022)

Subject Terms: G protein-coupled receptors (GPCRs), heteromer, dimer, biased signalling, β-arrestin, endocytosis, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fendo.2022.931573/full; https://doaj.org/toc/1664-2392

Access URL: https://doaj.org/article/bca4f33656444636bc98420ffa0346bc

The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer

Authors: Emily Golden, Rabab Rashwan, Eleanor A. Woodward, Agustin Sgro, Edina Wang, Anabel Sorolla, Charlene Waryah, Wan Jun Tie, Elisabet Cuyàs, Magdalena Ratajska, Iwona Kardaś, Piotr Kozlowski, Elizabeth K. M. Johnstone, Heng B. See, Ciara Duffy, Jeremy Parry, Kim A. Lagerborg, Piotr Czapiewski, Javier A. Menendez, Adam Gorczyński, Bartosz Wasag, Kevin D. G. Pfleger, Christina Curtis, Bum-Kyu Lee, Jonghwan Kim, Joseph Cursons, Nathan J. Pavlos, Wojciech Biernat, Mohit Jain, Andrew J. Woo, Andrew Redfern, Pilar Blancafort

Source: Nature Communications, Vol 12, Iss 1, Pp 1-22 (2021)

Subject Terms: Science

File Description: electronic resource

Relation: https://doaj.org/toc/2041-1723

Access URL: https://doaj.org/article/7a78efc95f94434186efe33c5a90c19a

Novel Pharmacology Following Heteromerization of the Angiotensin II Type 2 Receptor and the Bradykinin Type 2 Receptor

Authors: Elizabeth K. M. Johnstone, Mohammed Akli Ayoub, Rebecca J. Hertzman, Heng B. See, Rekhati S. Abhayawardana, Ruth M. Seeber, Kevin D. G. Pfleger

Source: Frontiers in Endocrinology, Vol 13 (2022)

Subject Terms: angiotensin receptor, bradykinin receptor, GPCR, BRET, receptor-HIT, heteromer, Diseases of the endocrine glands. Clinical endocrinology, RC648-665

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fendo.2022.848816/full; https://doaj.org/toc/1664-2392

Access URL: https://doaj.org/article/05b7a90cfa2845508b41819310d3747e

Orexin Signaling: A Complex, Multifaceted Process

Authors: Natasha C. Dale, Daniel Hoyer, Laura H. Jacobson, Kevin D. G. Pfleger, Elizabeth K. M. Johnstone

Source: Frontiers in Cellular Neuroscience, Vol 16 (2022)

Subject Terms: orexin, orexin 1 receptor, orexin 2 receptor, signaling, G protein, GPCR (G protein coupled receptor), Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fncel.2022.812359/full; https://doaj.org/toc/1662-5102

Access URL: https://doaj.org/article/1a03bd424f604276a66a0509a4f1b3c5

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Authors: Carl W. White, Hannah K. Vanyai, Heng B. See, Elizabeth K. M. Johnstone, Kevin D. G. Pfleger

Source: Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/7b3fcf6037e049f69f6a0bb8d24be1b0

NanoBRET: The Bright Future of Proximity-Based Assays

Authors: Natasha C. Dale, Elizabeth K. M. Johnstone, Carl W. White, Kevin D. G. Pfleger

Source: Frontiers in Bioengineering and Biotechnology, Vol 7 (2019)

Subject Terms: BRET, NanoLuc, Nluc, NanoBRET, fluorophore, ligand binding, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.frontiersin.org/article/10.3389/fbioe.2019.00056/full; https://doaj.org/toc/2296-4185

Access URL: https://doaj.org/article/af415a5501e44f34a985747f9c785f07