Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging

Authors: Hickey, John W., Neumann, Elizabeth K., Radtke, Andrea J., Camarillo, Jeannie M., Beuschel, Rebecca T., Albanese, Alexandre, McDonough, Elizabeth, Hatler, Julia, Wiblin, Anne E., Fisher, Jeremy, Croteau, Josh, Small, Eliza C., Sood, Anup, Caprioli, Richard M., Angelo, R. Michael, Nolan, Garry P., Chung, Kwanghun, Hewitt, Stephen M., Germain, Ronald N., Spraggins, Jeffrey M., Lundberg, Emma, Snyder, Michael P., Kelleher, Neil L., Saka, Sinem K.

Subject Terms: Quantitative Biology - Tissues and Organs

Access URL: http://arxiv.org/abs/2107.07953

Quality Assessment of Proteins and RNA Following Storage in Archival Formalin-Fixed Paraffin-Embedded Human Breast Cancer Tissue Microarray Sections

Authors: Kim, Kyungeun, Ylaya, Kris, Perry, Candice, Lee, Mi-Yeon, Kim, Jeong Won, Chung, Joon-Yong, Hewitt, Stephen M.

Source: Biopreservation and biobanking. 21(5):493-503

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100196347838.0x000001&indx=1&recIds=ETOCvdc_100196347838.0x000001

Histopathology and SARS-CoV-2 Cellular Localization in Eye Tissues of COVID-19 Autopsies

Authors: Stein, Sydney R., Ramelli, Sabrina C., Ramos-Benitez, Marcos J., Platt, Andrew P., Dickey, James M., Curran, Shelly J., Babyak, Ashley L., Valencia, Luis Perez, Richert, Mary E., Vannella, Kevin M., Chertow, Daniel S., Kleiner, David E., Hewitt, Stephen M., Young, Willie J., Young, Sarah P., Gasmi, Billel, De Melo, Michelly Sampaio, Desar, Sabina, Tadros, Saber, Nasir, Nadia, Jin, Xueting, Rajan, Sharika, Dikoglu, Esra, Ozkaya, Neval, Ylaya, Kris, Chung, Joon-Yong, Pittaluga, Stefania, Smith, Grace, Emanuel, Elizabeth R., Kelsall, Brian, Olivera, Justin A., Blawas, Megan, Grazioli, Alison, Hays, Nicole, Purcell, Madeleine, Singireddy, Shreya, Wu, Jocelyn, Raja, Katherine, Curto, Ryan, Chung, Jean, Borth, Amy, Bowers, Kimberly, Weichold, Anne, Minor, Paula, Moshref, Mirahmad, Kelly, Emily, Sajadi, Mohammad M., Scalea, Thomas M., Tran, Douglas, Madathil, Ronson J., Dahi, Siamak, Deatrick, Kristopher B., Krause, Eric M., Rabin, Joseph, Herrold, Joseph A., Tabatabai, Ali, Hochberg, Eric, Cornachione, Christopher, Levine, Andrea R., Saharia, Kapil K., Richards, Justin E., Elder, John, Burke, Allen, Mazzeffi, Michael A., Christenson, Robert, Chancer, Zackary, Abdulmahdi, Mustafa, Sopha, Sabrina, Goldberg, Tyler, Soherwardi, Shahabuddin, Sangwan, Yashvir, McCurdy, Michael T., Sudano, Kristen, Blume, Diane, Radin, Bethany, Arnouk, Madhat, Eagan, James W., Jr., Herr, Daniel L., Zerbe, Christa, Sen, H. Nida, Wang, Yujuan, Kodati, Shilpa, Lee, Jung Wha, Perez, Paola, Singh, Manmeet, Yinda, Kwe Claude, Platt, Andrew, Munster, Vincent J., de Wit, Emmie, Warner, Blake M., Chan, Chi-Chao

Source: In The American Journal of Pathology November 2023 193(11):1809-1816

SARS-CoV-2 infection of salivary glands Compromises the production of a secreted antifungal peptide with potential implications for Development of oral candidiasis.

Authors: Alfaifi, Areej A.^1,2,3, Wang, Tristan W.¹, Perez, Paola⁴, Sultan, Ahmed S.^1,5, Meiller, Timothy F.^1,5, Rock, Peter⁶, Kleiner, David E.⁷, Chertow, Daniel S.^8,9, Hewitt, Stephen M.⁷, Gasmi, Billel⁷, Stein, Sydney⁸, Ramelli, Sabrina⁸, Martin, Daniel¹⁰, Warner, Blake M.⁴, blake.warner@nih.gov, Jabra-Rizk, Mary Ann^1,11, mrizk@umaryland.edu

Source: PLoS Pathogens; 12/12/2024, Vol. 20 Issue 12, p1-12, 12p

Systemic Nos2 Depletion and Cox inhibition limits TNBC disease progression and alters lymphoid cell spatial orientation and density

Authors: Somasundaram, Veena, Ridnour, Lisa A., Cheng, Robert YS., Walke, Abigail J., Kedei, Noemi, Bhattacharyya, Dibyangana D., Wink, Adelaide L., Edmondson, Elijah F., Butcher, Donna, Warner, Andrew C., Dorsey, Tiffany H., Scheiblin, David A., Heinz, William, Bryant, Richard J., Kinders, Robert J., Lipkowitz, Stanley, Wong, Stephen TC., Pore, Milind, Hewitt, Stephen M., McVicar, Daniel W., Anderson, Stephen K., Chang, Jenny, Glynn, Sharon A., Ambs, Stefan, Lockett, Stephen J., Wink, David A.

Source: In Redox Biology December 2022 58

The role of FXR and TGR5 in reversing and preventing progression of Western diet–induced hepatic steatosis, inflammation, and fibrosis in mice

Authors: Wang, Xiaoxin X., Xie, Cen, Libby, Andrew E., Ranjit, Suman, Levi, Jonathan, Myakala, Komuraiah, Bhasin, Kanchan, Jones, Bryce A., Orlicky, David J., Takahashi, Shogo, Dvornikov, Alexander, Kleiner, David E., Hewitt, Stephen M., Adorini, Luciano, Kopp, Jeffrey B., Krausz, Kristopher W., Rosenberg, Avi, McManaman, James L., Robertson, Charles E., Ir, Diana, Frank, Daniel N., Luo, Yuhuan, Gonzalez, Frank J., Gratton, Enrico, Levi, Moshe

Source: In Journal of Biological Chemistry November 2022 298(11)

Single‐cell biology uncovers apoptotic cell death and its spatial organization as a potential modifier of tumor diversity in HCC

Authors: Khatib, Subreen A., Ma, Lichun, Dang, Hien, Forgues, Marshonna, Chung, Joon‐Yong, Ylaya, Kris, Hewitt, Stephen M., Chaisaingmongkol, Jittporn, Rucchirawat, Mathuros, Wang, Xin Wei

Source: Hepatology. 76(3):599-611

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100191467672.0x000001&indx=1&recIds=ETOCvdc_100191467672.0x000001

Reproducible, high-dimensional imaging in archival human tissue by multiplexed ion beam imaging by time-of-flight (MIBI-TOF)

Authors: Liu, Candace C., Bosse, Marc, Kong, Alex, Kagel, Adam, Kinders, Robert, Hewitt, Stephen M., Varma, Sushama, van de Rijn, Matt, Nowak, Stanisław H., Bendall, Sean C., Angelo, Michael

Source: In Laboratory Investigation July 2022 102(7):762-770

DNA methylation patterns are influenced by Pax3::Foxo1 expression and developmental lineage in rhabdomyosarcoma tumours forming in genetically engineered mouse models.

Authors: Sun, Wenyue, Hewitt, Stephen M, Wright, Hollis, Keller, Charles, Barr, Frederic G

Source: Journal of Pathology; Mar2025, Vol. 265 Issue 3, p316-329, 14p

Subject Terms: DNA methylation, RHABDOMYOSARCOMA, TRANSGENIC mice, CHIMERIC proteins, GENE fusion, GENETIC mutation, MYOBLASTS, GENETIC regulation

CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice

Authors: Li, Nan, Torres, Madeline B., Spetz, Madeline R., Wang, Ruixue, Peng, Luyi, Tian, Meijie, Dower, Christopher M., Nguyen, Rosa, Sun, Ming, Tai, Chin-Hsien, de Val, Natalia, Cachau, Raul, Wu, Xiaolin, Hewitt, Stephen M., Kaplan, Rosandra N., Khan, Javed, St Croix, Brad, Thiele, Carol J., Ho, Mitchell

Source: In Cell Reports Medicine 15 June 2021 2(6)

Improving data quality in observational research studies: Report of the Cure Glomerulonephropathy (CureGN) network

Authors: Gillespie, Brenda W., Laurin, Louis-Philippe, Zinsser, Dawn, Lafayette, Richard, Marasa, Maddalena, Wenderfer, Scott E., Vento, Suzanne, Poulton, Caroline, Barisoni, Laura, Zee, Jarcy, Helmuth, Margaret, Lugani, Francesca, Kamel, Margret, Hill-Callahan, Peg, Hewitt, Stephen M., Mariani, Laura H., Smoyer, William E., Greenbaum, Larry A., Gipson, Debbie S., Robinson, Bruce M., Gharavi, Ali G., Guay-Woodford, Lisa M., Trachtman, Howard

Source: In Contemporary Clinical Trials Communications June 2021 22

Development of CAR T Cells Expressing a Suicide Gene Plus a Chimeric Antigen Receptor Targeting Signaling Lymphocytic-Activation Molecule F7

Authors: Amatya, Christina, Pegues, Melissa A., Lam, Norris, Vanasse, Danielle, Geldres, Claudia, Choi, Stephanie, Hewitt, Stephen M., Feldman, Steven A., Kochenderfer, James N.

Source: In Molecular Therapy 3 February 2021 29(2):702-717

Development and evaluation of deep learning–based segmentation of histologic structures in the kidney cortex with multiple histologic stains

Authors: Sedor, J., Dell, K., Schachere, M., Negrey, J., Lemley, K., Lim, E., Srivastava, T., Garrett, A., Sethna, C., Laurent, K., Appel, G., Toledo, M., Barisoni, L., Greenbaum, L., Wang, C., Kang, C., Adler, S., Nast, C., LaPage, J., Stroger, John H., Jr., Athavale, A., Itteera, M., Neu, A., Boynton, S., Fervenza, F., Hogan, M., Lieske, J., Chernitskiy, V., Kaskel, F., Kumar, N., Flynn, P., Kopp, J., Blake, J., Trachtman, H., Zhdanova, O., Modersitzki, F., Vento, S., Lafayette, R., Mehta, K., Gadegbeku, C., Johnstone, D., Quinn-Boyle, S., Cattran, D., Hladunewich, M., Reich, H., Ling, P., Romano, M., Fornoni, A., Bidot, C., Kretzler, M., Gipson, D., Williams, A., LaVigne, J., Derebail, V., Gibson, K., Froment, A., Grubbs, S., Holzman, L., Meyers, K., Kallem, K., Lalli, J., Sambandam, K., Wang, Z., Rogers, M., Jefferson, A., Hingorani, S., Tuttle, K., Bray, M., Kelton, M., Cooper, A., Freedman, B., Lin, J.J., Jayapandian, Catherine P., Chen, Yijiang, Janowczyk, Andrew R., Palmer, Matthew B., Cassol, Clarissa A., Sekulic, Miroslav, Hodgin, Jeffrey B., Zee, Jarcy, Hewitt, Stephen M., O’Toole, John, Toro, Paula, Sedor, John R., Barisoni, Laura, Madabhushi, Anant

Source: In Kidney International January 2021 99(1):86-101

Development and evaluation of deep learning–based segmentation of histologic structures in the kidney cortex with multiple histologic stains

Authors: Jayapandian, Catherine P., Chen, Yijiang, Janowczyk, Andrew R., Palmer, Matthew B., Cassol, Clarissa A., Sekulic, Miroslav, Hodgin, Jeffrey B., Zee, Jarcy, Hewitt, Stephen M., O'Toole, John, Toro, Paula, Sedor, John R., Barisoni, Laura, Madabhushi, Anant

Source: Kidney international. 99(1):86-101

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100136183806.0x000001&indx=1&recIds=ETOCvdc_100136183806.0x000001

Intratumoral γδ T‐Cell Infiltrates, Chemokine (C‐C Motif) Ligand 4/Chemokine (C‐C Motif) Ligand 5 Protein Expression and Survival in Patients With Hepatocellular Carcinoma

Authors: Zhao, Na, Dang, Hien, Ma, Lichun, Martin, Sean P., Forgues, Marshonna, Ylaya, Kris, Hewitt, Stephen M., Wang, Xin Wei

Source: Hepatology. 73(3):1045-1060

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100116945917.0x000001&indx=1&recIds=ETOCvdc_100116945917.0x000001

LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells

Authors: Kim, Suyeon, Cho, Hanbyoul, Hong, Soon-Oh, Oh, Se Jin, Lee, Hyo-Jung, Cho, Eunho, Woo, Seon Rang, Song, Joon Seon, Chung, Joon-Yong, Son, Sung Wook, Yoon, Sang Min, Jeon, Yu-Min, Jeon, Seunghyun, Yee, Cassian, Lee, Kyung-Mi, Hewitt, Stephen M., Kim, Jae-Hoon, Song, Kwon-Ho, Kim, Tae Woo

Source: Autophagy. 17(8):1978-1997

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100162802989.0x000001&indx=1&recIds=ETOCvdc_100162802989.0x000001

The Use of Quantitative Digital Pathology to Measure Proteoglycan and Glycosaminoglycan Expression and Accumulation in Healthy and Diseased Tissues

Authors: Davis, A. Sally, Chang, Mary Y., Brune, Jourdan E., Hallstrand, Teal S., Johnson, Brian, Lindhartsen, Sarah, Hewitt, Stephen M., Frevert, Charles W.

Source: Journal of histochemistry and cytochemistry. 69(2):137-155

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN626453465&indx=1&recIds=ETOCRN626453465

Persistent Polyfunctional Chimeric Antigen Receptor T Cells That Target Glypican 3 Eliminate Orthotopic Hepatocellular Carcinomas in Mice

Authors: Li, Dan, Li, Nan, Zhang, Yi-Fan, Fu, Haiying, Feng, Mingqian, Schneider, Dina, Su, Ling, Wu, Xiaolin, Zhou, Jing, Mackay, Sean, Kramer, Josh, Duan, Zhijian, Yang, Hongjia, Kolluri, Aarti, Hummer, Alissa M., Torres, Madeline B., Zhu, Hu, Hall, Matthew D., Luo, Xiaoling, Chen, Jinqiu, Wang, Qun, Abate-Daga, Daniel, Dropulic, Boro, Hewitt, Stephen M., Orentas, Rimas J., Greten, Tim F., Ho, Mitchell

Source: In Gastroenterology June 2020 158(8):2250-2265

Chemoradiotherapy response prediction model by proteomic expressional profiling in patients with locally advanced cervical cancer

Authors: Choi, Chel Hun, Chung, Joon-Yong, Kang, Jun Hyeok, Paik, E. Sun, Lee, Yoo-Young, Park, Won, Byeon, Sun-Ju, Chung, Eun Joo, Kim, Byoung-Gie, Hewitt, Stephen M., Bae, Duk-Soo

Source: In Gynecologic Oncology May 2020 157(2):437-443

Cell-specific cargo delivery using synthetic bacterial spores

Authors: Kong, Minsuk, D'Atri, Domenico, Bilotta, Maria Teresa, Johnson, Bailey, Updegrove, Taylor B., Gallardo, Devorah L., Machinandiarena, Federico, Wu, I-Lin, Constantino, Maira Alves, Hewitt, Stephen M., Tanner, Kandice, Fitzgerald, David J., Ramamurthi, Kumaran S.

Source: Cell reports. 42(1)

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100186678219.0x000001&indx=1&recIds=ETOCvdc_100186678219.0x000001