Super-resolution fluorescence microscopy and cryogenic soft X-ray tomography enable intracellular localization of stable nanoparticles

Authors: Ribeiro, Iris R.S. ^a, Harkiolaki, Maria ^{b, c}, S. Plivelic, Tomás ^d, Barbosa, Leandro R.S. ^{a, e, ⁎}

Source: In Nano Today April 2025 61

Correlative cryo-soft X-ray tomography and cryo-structured illumination microscopy reveal changes to lysosomes in amyloid-β-treated neurons

Authors: Marshall, Karen E., Mengham, Kurtis, Spink, Matthew C., Vania, Lyra, Pollard, Hannah Jane, Darrow, Michele C., Duke, Elizabeth, Harkiolaki, Maria, Serpell, Louise C.

Source: In Structure 2 May 2024 32(5):585-593

Correlative light and X-ray tomography jointly unveil the critical role of connexin43 channels on inflammation-induced cellular ultrastructural alterations

Authors: Okolo, Chidinma Adanna ^{a, ⁎, 1}, Maran, Jack Jonathan ^b, Watts, Amy ^a, Maripillan, Jaime ^c, Harkiolaki, Maria ^a, Martínez, Agustín D. ^c, Green, Colin R. ^d, Mugisho, Odunayo Omolola ^b

Source: In Heliyon 15 April 2024 10(7)

Epitope length variants balance protective immune responses and viral escape in HIV-1 infection

Authors: Pymm, Phillip, Tenzer, Stefan, Wee, Edmund, Weimershaus, Mirjana, Burgevin, Anne, Kollnberger, Simon, Gerstoft, Jan, Josephs, Tracy M., Ladell, Kristin, McLaren, James E., Appay, Victor, Price, David A., Fugger, Lars, Bell, John I., Schild, Hansjörg, van Endert, Peter, Harkiolaki, Maria, Iversen, Astrid K.N.

Source: In Cell Reports 1 March 2022 38(9)

Progression of herpesvirus infection remodels mitochondrial organization and metabolism.

Authors: Leclerc, Simon¹, Gupta, Alka¹, Ruokolainen, Visa¹, Chen, Jian-Hua², Kunnas, Kari¹, Ekman, Axel A.^1,2, Niskanen, Henri³, Belevich, Ilya⁴, Vihinen, Helena⁴, Turkki, Paula⁵, Perez-Berna, Ana J.⁶, Kapishnikov, Sergey⁷, Mäntylä, Elina⁵, Harkiolaki, Maria⁸, Dufour, Eric⁵, Hytönen, Vesa^5,9, Pereiro, Eva⁶, McEnroe, Tony⁷, Fahy, Kenneth⁷, Kaikkonen, Minna U.³

Source: PLoS Pathogens; 4/15/2024, Vol. 20 Issue 4, p1-28, 28p

Single-Cell Chemistry of Photoactivatable Platinum Anticancer Complexes

Authors: Bolitho, Elizabeth M., Sanchez-Cano, Carlos, Shi, Huayun, Quinn, Paul D., Harkiolaki, Maria, Imberti, Cinzia, Sadler, Peter J.

Source: Journal of the American Chemical Society. 143(48):20224-20240

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN628303779&indx=1&recIds=ETOCRN628303779

Correlative cryo‐imaging of the cellular universe with soft X‐rays and laser light used to track F‐actin structures in mammalian cells

Authors: Koronfel, Mohamed, Kounatidis, Ilias, Mwangangi, Dennis M., Vyas, Nina, Okolo, Chidinma, Jadhav, Archana, Fish, Tom, Chotchuang, Phatcharin, Schulte, Albert, Robinson, Robert C., Harkiolaki, Maria

Source: Acta crystallographica.Section D.Structural biology. 77(12):1479-1485

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100135982146.0x000001&indx=1&recIds=ETOCvdc_100135982146.0x000001

Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development

Authors: Okolo, Chidinma A., Jadhav, Archana, Phillips, Patrick, Dumoux, Maud, McMurray, Amanda A., Joshi, Vishwas D., Pizzey, Claire, Harkiolaki, Maria

Source: Journal of microscopy. 284(3):214-232

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100134619460.0x000001&indx=1&recIds=ETOCvdc_100134619460.0x000001

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

Authors: Kounatidis, Ilias, Stanifer, Megan L., Phillips, Michael A., Paul-Gilloteaux, Perrine, Heiligenstein, Xavier, Wang, Hongchang, Okolo, Chidinma A., Fish, Thomas M., Spink, Matthew C., Stuart, David I., Davis, Ilan, Boulant, Steeve, Grimes, Jonathan M., Dobbie, Ian M., Harkiolaki, Maria

Source: In Cell 23 July 2020 182(2):515-530

Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila

Authors: Vaz, Filipa, Kounatidis, Ilias, Covas, Gonçalo, Parton, Richard M., Harkiolaki, Maria, Davis, Ilan, Filipe, Sergio Raposo, Ligoxygakis, Petros

Source: In Cell Reports 21 May 2019 27(8):2480-2492

An Integrated Approach to Elucidate the Interplay between Iron Uptake Dynamics and Magnetosome Formation at the Single-Cell Level in Magnetospirillum gryphiswaldense

Authors: Masó-Martínez, Marta, Bond, Josh, Okolo, Chidinma A, Jadhav, Archana C, Harkiolaki, Maria, Topham, Paul D, Fernández-Castané, Alfred

Source: ACS Applied Materials & Interfaces; November 2024, Vol. 16 Issue: 45 p62557-62570, 14p

X-ray tomography of cryopreserved human prostate cancer cells: mitochondrial targeting by an organoiridium photosensitiser

Authors: Bolitho, Elizabeth M., Sanchez-Cano, Carlos, Huang, Huaiyi, Hands-Portman, Ian, Spink, Matthew, Quinn, Paul D., Harkiolaki, Maria, Sadler, Peter J.

Source: Journal of biological inorganic chemistry. 25(2):295-303

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100164401802.0x000001&indx=1&recIds=ETOCvdc_100164401802.0x000001

Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress

Authors: Hale, Victoria L., Watermeyer, Jean M., Hackett, Fiona, Vizcay-Barrena, Gema, van Ooij, Christiaan, Thomas, James A., Spink, Matthew C., Harkiolaki, Maria, Duke, Elizabeth, Fleck, Roland A., Blackman, Michael J., Saibil, Helen R.

Source: Proceedings of the National Academy of Sciences of the United States of America, 2017 Mar . 114(13), 3439-3444.

Access URL: https://www.jstor.org/stable/26480343

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection.

Authors: Nahas, Kamal L.^1,2, Connor, Viv¹, Scherer, Katharina M.³, Kaminski, Clemens F.³, Harkiolaki, Maria², maria.harkiolaki@diamond.ac.uk, Crump, Colin M.¹, maria.harkiolaki@diamond.ac.uk, Graham, Stephen C.¹, maria.harkiolaki@diamond.ac.uk

Source: PLoS Pathogens; 7/7/2022, Vol. 18 Issue 7, p1-34, 34p

Compensation of x-ray mirror distortion by cooling temperature control

Authors: Hand, Matthew, Wang, Hongchang, Harkiolaki, Maria, Venturini, Federica, Arrigo, Rosa, Ferrer-Escorihuela, Pilar, Alcock, Simon, Nistea, Ioana, Marshall, Andy, Scott, Stewart, Duke, Liz, Held, Georg, Sawhney, Kawal

Source: AIP conference proceedings. 2054

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100090577379.0x000001&indx=1&recIds=ETOCvdc_100090577379.0x000001

Distinct Binding Modes of Two Epitopes in Gab2 that Interact with the SH3C Domain of Grb2

Authors: Harkiolaki, Maria, Tsirka, Theodora, Lewitzky, Marc, Simister, Philip C., Joshi, Dhira, Bird, Louise E., Jones, E. Yvonne, O'Reilly, Nicola, Feller, Stephan M.

Source: In Structure 10 June 2009 17(6):809-822

T Cell-Mediated Autoimmune Disease Due to Low-Affinity Crossreactivity to Common Microbial Peptides

Authors: Harkiolaki, Maria, Holmes, Samantha L., Svendsen, Pia, Gregersen, Jon W., Jensen, Lise T., McMahon, Roisin, Friese, Manuel A., van Boxel, Gijs, Etzensperger, Ruth, Tzartos, John S., Kranc, Kamil, Sainsbury, Sarah, Harlos, Karl, Mellins, Elizabeth D., Palace, Jackie, Esiri, Margaret M., van der Merwe, P. Anton, Jones, E. Yvonne, Fugger, Lars

Source: In Immunity 2009 30(3):348-357

Crystal Structure and DNA Repair Activities of the AP Endonuclease from Leishmania major

Authors: Vidal, Antonio E. ^†, Harkiolaki, Maria ^{†, ‡}, Gallego, Claribel, Castillo-Acosta, Victor M., Ruiz-Pérez, Luis M., Wilson, Keith, González-Pacanowska, Dolores

Source: In Journal of Molecular Biology 2007 373(4):827-838

The C-Terminal SH3 Domain of CRKL as a Dynamic Dimerization Module Transiently Exposing a Nuclear Export Signal

Authors: Harkiolaki, Maria, Gilbert, Robert J.C., Jones, E. Yvonne, Feller, Stephan M.

Source: In Structure 2006 14(12):1741-1753

Mona/Gads SH3C Binding to Hematopoietic Progenitor Kinase 1 (HPK1) Combines an Atypical SH3 Binding Motif, R/KXXK, with a Classical PXXP Motif Embedded in a Polyproline Type II (PPII) Helix

Authors: Lewitzky, Marc ^{‡, §}, Harkiolaki, Maria ^{‡, §}, Domart, Marie-Charlotte ^¶, Jones, E. Yvonne ^{∥, **}, Feller, Stephan M. ^{‡, ‡‡}

Source: In Journal of Biological Chemistry 2 July 2004 279(27):28724-28732