Adaptive laboratory evolution and genetic engineering improved terephthalate utilization in Pseudomonas putida KT2440

Authors: Werner, Allison Z. ^{a, b}, Avina, Young-Saeng C. ^{a, b, 1}, Johnsen, Josefin ^c, Bratti, Felicia ^{a, b}, Alt, Hannah M. ^a, Mohamed, Elsayed T. ^c, Clare, Rita ^{a, b}, Mand, Thomas D. ^{b, d}, Guss, Adam M. ^{b, d}, Feist, Adam M. ^{c, e, ⁎⁎}, Beckham, Gregg T. ^{a, b, ⁎}

Source: In Metabolic Engineering March 2025 88:196-205

The tier system: a host development framework for bioengineering

Authors: Yeager, Chris M ^{1, 2}, Hillson, Nathan J ^{1, 3}, Wozniak, Katherine L ^{1, 2}, Mutalik, Vivek K ^{1, 4}, Johnson, Christopher W ^{1, 5}, Pomraning, Kyle ^{1, 6}, Laible, Philip ^{1, 7}, Dale, Taraka ^{1, 8}, Guss, Adam M ^{1, 9}

Source: In Current Opinion in Biotechnology April 2025 92

Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440

Authors: Bleem, Alissa C., Kuatsjah, Eugene, Johnsen, Josefin, Mohamed, Elsayed T., Alexander, William G., Kellermyer, Zoe A., Carroll, Austin L., Rossi, Riccardo, Schlander, Ian B., Peabody V, George L., Guss, Adam M., Feist, Adam M., Beckham, Gregg T.

Source: In Metabolic Engineering July 2024 84:145-157

Machine-learning from Pseudomonas putida KT2440 transcriptomes reveals its transcriptional regulatory network

Authors: Lim, Hyun Gyu, Rychel, Kevin, Sastry, Anand V., Bentley, Gayle J., Mueller, Joshua, Schindel, Heidi S., Larsen, Peter E., Laible, Philip D., Guss, Adam M., Niu, Wei, Johnson, Christopher W., Beckham, Gregg T., Feist, Adam M., Palsson, Bernhard O.

Source: METABOLIC ENGINEERING. 72:297-310

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100167427879.0x000001&indx=1&recIds=ETOCvdc_100167427879.0x000001

Toward low-cost biological and hybrid biological/catalytic conversion of cellulosic biomass to fuelsElectronic supplementary information (ESI) available. See DOI: 10.1039/d1ee02540f

Authors: Lynd, Lee R., Beckham, Gregg T., Guss, Adam M., Jayakody, Lahiru N., Karp, Eric M., Maranas, Costas, McCormick, Robert L., Amador-Noguez, Daniel, Bomble, Yannick J., Davison, Brian H., Foster, Charles, Himmel, Michael E., Holwerda, Evert K., Laser, Mark S., Ng, Chiam Yu, Olson, Daniel G., Román-Leshkov, Yuriy, Trinh, Cong T., Tuskan, Gerald A., Upadhayay, Vikas, Vardon, Derek R., Wang, Lin, Wyman, Charles E.

Source: Energy & environmental science. 15(3):938-990

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100144689816.0x000001&indx=1&recIds=ETOCvdc_100144689816.0x000001

Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Authors: Werner, Allison Z., Clare, Rita, Mand, Thomas D., Pardo, Isabel, Ramirez, Kelsey J., Haugen, Stefan J., Bratti, Felicia, Dexter, Gara N., Elmore, Joshua R., Huenemann, Jay D., Peabody, George L., V, Johnson, Christopher W., Rorrer, Nicholas A., Salvachúa, Davinia, Guss, Adam M., Beckham, Gregg T.

Source: In Metabolic Engineering September 2021 67:250-261

Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid

Authors: Notonier, Sandra, Werner, Allison Z., Kuatsjah, Eugene, Dumalo, Linda, Abraham, Paul E., Hatmaker, E. Anne, Hoyt, Caroline B., Amore, Antonella, Ramirez, Kelsey J., Woodworth, Sean P., Klingeman, Dawn M., Giannone, Richard J., Guss, Adam M., Hettich, Robert L., Eltis, Lindsay D., Johnson, Christopher W., Beckham, Gregg T.

Source: In Metabolic Engineering May 2021 65:111-122

Comparison of microbial strains as candidate hosts and genetic reservoirs for the valorization of lignin streams.

Authors: Wilkes, Rebecca A., Borchert, Andrew J., Garcia, Valentina E., Geiselman, Gina M., Liu, Sarah, Guss, Adam M., Michener, Joshua K., Noguera, Daniel R., Masai, Eiji, Gladden, John M., Ralph, John, Beckham, Gregg T.

Source: Green Chemistry; 12/21/2024, Vol. 26 Issue 24, p12053-12069, 17p

Subject Terms: CORN stover, CHEMICAL models, ALIPHATIC compounds, PSEUDOMONAS putida, AROMATIC compounds, LIGNIN structure

Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers.

Authors: Sharma, Bishal Dev, Hon, Shuen, Thusoo, Eashant, Stevenson, David M., Amador-Noguez, Daniel, Guss, Adam M., Lynd, Lee R., Olson, Daniel G.

Source: Biotechnology for Biofuels & Bioproducts; 12/18/2024, Vol. 17 Issue 1, p1-15, 15p

Subject Terms: CLOSTRIDIUM thermocellum, THERMODYNAMICS, PYRUVATE kinase, GLYCOLYSIS, THERMAL engineering, ETHANOL

Mechanisms of Polyethylene Terephthalate Pellet Fragmentation into Nanoplastics and Assimilable Carbons by Wastewater Comamonas.

Authors: Wilkes, Rebecca A., Zhou, Nanqing, Carroll, Austin L., Aryal, Ojaswi, Teitel, Kelly P., Wilson, Rebecca S., Zhang, Lichun, Kapoor, Arushi, Castaneda, Edgar, Guss, Adam M., Waldbauer, Jacob R., Aristilde, Ludmilla

Source: Environmental Science & Technology; 10/29/2024, Vol. 58 Issue 43, p19338-19352, 15p

Engineered Pseudomonas putida simultaneously catabolizes five major components of corn stover lignocellulose: Glucose, xylose, arabinose, p-coumaric acid, and acetic acid

Authors: Elmore, Joshua R., Dexter, Gara N., Salvachúa, Davinia, O'Brien, Marykate, Klingeman, Dawn M., Gorday, Kent, Michener, Joshua K., Peterson, Darren J., Beckham, Gregg T., Guss, Adam M.

Source: In Metabolic Engineering November 2020 62:62-71

Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440

Authors: Bentley, Gayle J., Narayanan, Niju, Jha, Ramesh K., Salvachúa, Davinia, Elmore, Joshua R., Peabody, George L., Black, Brenna A., Ramirez, Kelsey, De Capite, Annette, Michener, William E., Werner, Allison Z., Klingeman, Dawn M., Schindel, Heidi S., Nelson, Robert, Foust, Lindsey, Guss, Adam M., Dale, Taraka, Johnson, Christopher W., Beckham, Gregg T.

Source: In Metabolic Engineering May 2020 59:64-75

Innovative Chemicals and Materials from Bacterial Aromatic Catabolic Pathways

Authors: Johnson, Christopher W., Salvachúa, Davinia, Rorrer, Nicholas A., Black, Brenna A., Vardon, Derek R., St. John, Peter C., Cleveland, Nicholas S., Dominick, Graham, Elmore, Joshua R., Grundl, Nicholas, Khanna, Payal, Martinez, Chelsea R., Michener, William E., Peterson, Darren J., Ramirez, Kelsey J., Singh, Priyanka, VanderWall, Todd A., Wilson, A. Nolan, Yi, Xiunan, Biddy, Mary J., Bomble, Yannick J., Guss, Adam M., Beckham, Gregg T.

Source: In Joule 19 June 2019 3(6):1523-1537

Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin

Authors: Salvachúa, Davinia, Rydzak, Thomas, Auwae, Raquel, De Capite, Annette, Black, Brenna A., Bouvier, Jason T., Cleveland, Nicholas S., Elmore, Joshua R., Huenemann, Jay D., Katahira, Rui, Michener, William E., Peterson, Darren J., Rohrer, Holly, Vardon, Derek R., Beckham, Gregg T., Guss, Adam M.

Source: Microbial biotechnology. 13(1):290-298

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100096272941.0x000001&indx=1&recIds=ETOCvdc_100096272941.0x000001

Corrigendum to “Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440” [Metab. Eng. 59 (2020) 64–75]

Authors: Bentley, Gayle J., Narayanan, Niju, Jha, Ramesh K., Salvachúa, Davinia, Elmore, Joshua R., Peabody, George L., Black, Brenna A., Ramirez, Kelsey, De Capite, Annette, Michener, William E., Werner, Allison Z., Klingeman, Dawn M., Schindel, Heidi S., Nelson, Robert, Foust, Lindsey, Guss, Adam M., Dale, Taraka, Johnson, Christopher W., Beckham, Gregg T.

Source: In Metabolic Engineering July 2022 72:66-67

Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction–modification systems

Authors: Riley, Lauren A., Ji, Lexiang, Schmitz, Robert J., Westpheling, Janet, Guss, Adam M.

Source: Journal of industrial microbiology & biotechnology. 46(9-10):1435-1443

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100118586987.0x000001&indx=1&recIds=ETOCvdc_100118586987.0x000001

Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440

Authors: Elmore, Joshua R., Furches, Anna, Wolff, Gara N., Gorday, Kent, Guss, Adam M.

Source: In Metabolic Engineering Communications December 2017 5:1-8

The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

Authors: Hon, Shuen, Olson, Daniel G., Holwerda, Evert K., Lanahan, Anthony A., Murphy, Sean J.L., Maloney, Marybeth I., Zheng, Tianyong, Papanek, Beth, Guss, Adam M., Lynd, Lee R.

Source: In Metabolic Engineering July 2017 42:175-184

Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellum

Authors: Rydzak, Thomas, Garcia, David, Stevenson, David M., Sladek, Margaret, Klingeman, Dawn M., Holwerda, Evert K., Amador-Noguez, Daniel, Brown, Steven D., Guss, Adam M.

Source: In Metabolic Engineering May 2017 41:182-191

Engineering electron metabolism to increase ethanol production in Clostridium thermocellum

Authors: Lo, Jonathan, Olson, Daniel G., Murphy, Sean Jean-Loup, Tian, Liang, Hon, Shuen, Lanahan, Anthony, Guss, Adam M., Lynd, Lee R.

Source: In Metabolic Engineering January 2017 39:71-79