Optimization of pan-lyssavirus LN34 assay for streamlined rabies diagnostics by real-time RT-PCR

Authors: Gigante, Crystal M. ^{a, ⁎}, Wicker, Vaughn ^a, Wilkins, Kimberly ^a, Seiders, Melanie ^b, Zhao, Hui ^a, Patel, Puja ^b, Orciari, Lillian ^a, Condori, Rene Edgar ^{a, c}, Dettinger, Lisa ^b, Yager, Pamela ^a, Xia, Dongxiang ^b, Li, Yu ^a

Source: In Journal of Virological Methods April 2025 333

Mpox Cluster Caused by Tecovirimat-Resistant Monkeypox Virus -- Five States, October 2023-February 2024.

Authors: Gigante, Crystal M.¹ lzu1@cdc.gov, Takakuwa, Jade¹, McGrath, Daisy¹, Kling, Chantal¹, Smith, Todd G.¹, Peng, Mengfei¹, Wilkins, Kimberly¹, Garrigues, Jacob M.², Holly, Taylor³, Barbian, Hannah⁴, Kittner, Alyse³, Haydel, Danielle⁵, Ortega, Emma⁵, Richardson, Gillian⁵, Hand, Julie⁵, Hacker, Jill K.⁶, Espinosa, Alex⁶, Haw, Monica⁶, Kath, Chantha⁶, Bielby, Meilan⁷

Source: MMWR: Morbidity & Mortality Weekly Report. 10/10/2024, Vol. 73 Issue 40, p903-905. 3p.

Subject Terms: *MONKEYPOX, *MONKEYPOX virus, *GENETIC mutation, *DRUG resistance in microorganisms, *THERAPEUTICS

First Clade Ib Monkeypox Virus Infection Reported in the Americas — California, November 2024.

Authors: Levy, Vivian, Branzuela, Anna, Hsieh, Kristina, Getabecha, Shiffen, III, Ricardo Berumen, Saadeh, Kayla, Snyder, Robert E., Marek, Gillian, Dodson, Daniel, Newman, Alyssa, Hacker, Jill K., Kath, Chantha, Minhaj, Faisal S., Gigante, Crystal M., Gearhart, Shannon, Kallen, Alexander, Hutson, Christina L., Jacobson, Kathleen

Source: MMWR: Morbidity & Mortality Weekly Report; 2/13/2025, Vol. 74 Issue 4, p44-49, 6p

Subject Terms: MONKEYPOX, LABORATORY reports, POLYMERASE chain reaction, PUBLIC health

Geographic Terms: CALIFORNIA, CONGO (Democratic Republic)

Monkeypox outbreak—Nine states, May 2022

Authors: Minhaj, Faisal S., Ogale, Yasmin P., Whitehill, Florence, Schultz, Jordan, Foote, Mary, Davidson, Whitni, Hughes, Christine M., Wilkins, Kimberly, Bachmann, Laura, Chatelain, Ryan, Donnelly, Marisa A. P., Mendoza, Rafael, Downes, Barbara L., Roskosky, Mellisa, Barnes, Meghan, Gallagher, Glen R., Basgoz, Nesli, Ruiz, Victoria, Kyaw, Nang Thu Thu, Feldpausch, Amanda, Valderrama, Amy, Alvarado‐Ramy, Francisco, Dowell, Chad H., Chow, Catherine C., Li, Yu, Quilter, Laura, Brooks, John, Daskalakis, Demetre C., McClung, R. Paul, Petersen, Brett W., Damon, Inger, Hutson, Christina, McQuiston, Jennifer, Rao, Agam K., Belay, Ermias, McCollum, Andrea M., Angelo, Kristina, Arduino, Matt, Arthur, Ray, Baird, Nicolle, Batross, Jonathan, Beeson, Amy, Bhingarde, Jui, Bowen, Michael, Brown, Clive, Brown, Catherine M., Burakoff, Alexis, Charniga, Kelly, Chen, Tai‐Ho, Chen, Sherry, Clay, Patrick, Cope, James, Cope, Jennifer, Dankwa, Michelle Addo, Delaney, Lisa, De Perio, Marie, Decenteceo, Michelle, Delea, Kristin, Doty, Jeffrey B., Duchin, Jeffrey, Dunlap, Joseph, Fagan, Ryan, Furness, Bryce, Gearhart, Shannon, Gigante, Crystal, Gilliland, Aubrey, Gosdin, Lucas, Griffin, Isabel, Groccia, Amanda, Guagliardo, Sarah, Hercules, Yonette, Jackson, Kelly, Jarquin, Paulino, Kachur, Rachel, Kallen, Alexander, Kao, Raymond, Kelly, Aubree, Khan, Mohammed, Khan, Theodora, Kofman, Aaron, Kornylo, Krista, Kuhar, David, LaFlam, Michael, Lash, Robert, Lashombe, Andrew, Lowe, David, MacGurn, Amanda, Masters, Nina, McCaffrey, Keegan, Mink, Jenna L., Monroe, Benjamin, Morgan, Clint N., Nakazawa, Yoshinori, Nash, Julie, Navarra, Terese, Newton, Donovan, Osinubi, Modupe, Osorio, Valentina, Pearson, Christine, Petras, Julia, Philpott, David, Pickrel, Amy, Potvin, Brandon, Priyamvada, Lalita, Rey, Araceli, Ricketts, Erin, Rodriguez, Sergio, Rushmore, Julie, Satheshkumar, Panayampalli S., Segaloff, Hannah, Sekkarie, Ahlia, Sharma, Artee, Sims, Emily, Smith, Dallas, Smith, Teresa, Smith, Todd, Solanky, Dipesh, Spiknall, Ian, Stanek, Danielle R., Stenger, Mark, Strona, Frank, Tardivel, Kara, Tyagi, Eishita, Wortley, Pascale, Valencia, Diana, Waltenburg, Michelle, Whitehouse, Erin, Wong, Marcia

Source: American journal of transplantation. 22(8):2104-2110

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100157724015.0x000001&indx=1&recIds=ETOCvdc_100157724015.0x000001

Tecovirimat Resistance in Mpox Patients, United States, 2022–2023.

Authors: Smith, Todd G.¹, Gigante, Crystal M.¹, Wynn, Nhien T.¹, Matheny, Audrey¹, Davidson, Whitni¹, Yong Yang², Condori, Rene Edgar¹, O’Connell, Kyle^1,2, Kovar, Lynsey³, Williams, Tracie L.¹, Yu, Yon C.¹, Petersen, Brett W.¹, Baird, Nicolle¹, Lowe, David¹, Yu Li¹, Satheshkumar, Panayampalli S.¹, Hutson, Christina L.¹

Source: Emerging Infectious Diseases. Dec2023, Vol. 29 Issue 12, p2426-2432. 7p.

Preventive Veterinary Medicine: Public Health Rabies surveillance in the United States during 2019

Authors: Ma, Xiaoyue, Monroe, Ben P., Wallace, Ryan M., Orciari, Lillian A., Gigante, Crystal M., Kirby, Jordona D., Chipman, Richard B., Fehlner-Gardiner, Christine, Cedillo, Veronica Gutiérrez, Petersen, Brett W.

Source: Journal of the American Veterinary Medical Association. 258(11):1205-1221

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN626726507&indx=1&recIds=ETOCRN626726507

Human Rabies — Utah, 2018

Authors: Peterson, Dallin, Barbeau, Bree, McCaffrey, Keegan, Gruninger, Randon, Eason, Jeffrey, Burnett, Cindy, Dunn, Angela, Dimond, Melissa, Harbour, Jesse, Rossi, Alessandro, Lopansri, Bert, Dascomb, Kristin, Scribellito, Tara, Moosman, TaLeah, Saw, Louise, Jones, Curtis, Belenky, Michael, Marsden, Lily, Niezgoda, Michael, Gigante, Crystal M., Condori, Rene Edgar, Ellison, James A., Orciari, Lillian A., Yager, Pamela, Bonwitt, Jesse, Whitehouse, Erin R., Wallace, Ryan M

Source: Morbidity and Mortality Weekly Report, 2020 Feb 01. 69(5), 121-124.

Access URL: https://www.jstor.org/stable/26908817

Public Veterinary Medicine: Public Health Rabies surveillance in the United States during 2018

Authors: Ma, Xiaoyue, Monroe, Ben P., Cleaton, Julie M., Orciari, Lillian A., Gigante, Crystal M., Kirby, Jordona D., Chipman, Richard B., Fehlner-Gardiner, Christine, Cedillo, Veronica Gutiérrez, Petersen, Brett W.

Source: Journal of the American Veterinary Medical Association. 256(2):195-209

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN622824228&indx=1&recIds=ETOCRN622824228

Human Rabies — Virginia, 2017

Authors: Murphy, Julia, Sifri, Costi D., Pruitt, Rhonda, Hornberger, Marcia, Bonds, Denise, Blanton, Jesse, Ellison, James, Cagnina, R. Elaine, Enfield, Kyle B., Shiferaw, Miriam, Gigante, Crystal, Condori, Edgar, Gruszynski, Karen, Wallace, Ryan M.

Source: Morbidity and Mortality Weekly Report, 2019 Jan 01. 67(51/52), 1410-1414.

Access URL: https://www.jstor.org/stable/26574339

Reemergence of a Big Brown Bat Lyssavirus rabies Variant in Striped Skunks in Flagstaff, Arizona, USA, 2021–2023.

Authors: Gilbert, Amy T., Van Pelt, Lolita I., Hastings, Lias A., Gigante, Crystal M., Orciari, Lillian A., Kelley, Sabrina, Fitzpatrick, Kathryn, Condori, Rene E. Condori, Li, Yu, Brunt, Scott, Davis, April, Hopken, Matthew W., Mankowski, Clara C. P., Wallace, Ryan M., Rupprecht, Charles E., Chipman, Richard B., Bergman, David L.

Source: Vector-Borne & Zoonotic Diseases; Aug2024, Vol. 24 Issue 8, p552-562, 11p

Subject Terms: RABIES vaccines, RABIES, VIRAL load, AUTUMN, ZOONOSES

Detection of Apparent Early Rabies Infection by LN34 Pan-Lyssavirus Real-Time RT-PCR Assay in Pennsylvania.

Authors: Dettinger, Lisa¹ (AUTHOR), Gigante, Crystal M.² (AUTHOR), Sellard, Maria¹ (AUTHOR), Seiders, Melanie¹ (AUTHOR), Patel, Puja¹ (AUTHOR), Orciari, Lillian A.² (AUTHOR), Yager, Pamela² (AUTHOR), Lute, James¹ (AUTHOR), Regec, Annette¹ (AUTHOR), Li, Yu² (AUTHOR) yuli@cdc.gov, Xia, Dongxiang¹ (AUTHOR) yuli@cdc.gov

Source: Viruses (1999-4915). Sep2022, Vol. 14 Issue 9, p1845-N.PAG. 11p.

Subject Terms: *RABIES, *FLUORESCENT antibody technique, *REVERSE transcriptase polymerase chain reaction, *RABIES virus, *BRAIN stem, *REVERSE transcriptase

Geographic Terms: PENNSYLVANIA

Company/Entity: CENTERS for Disease Control & Prevention (U.S.)

Divergent Rabies Virus Variant of Probable Bat Origin in 2 Gray Foxes, New Mexico, USA.

Authors: Condori, Rene E.¹, Aragon, Adam², Breckenridge, Mike², Pesko, Kendra², Mower, Kerry³, Ettestad, Paul², Melman, Sandra², Velasco-Villa, Andres¹, Orciari, Lillian A.¹, Yager, Pamela¹, Streicker, Daniel G.⁴, Gigante, Crystal M.¹, Morgan, Clint¹, Wallace, Ryan¹, euk5@cdc.gov, Li, Yu¹

Source: Emerging Infectious Diseases; Jun2022, Vol. 28 Issue 6, p1137-1145, 9p

Genetic Tracking of a Rabid Coyote (Canis latrans) Detected beyond a Rabies Enzootic Area in West Virginia, USA.

Authors: Hopken, Matthew W., Gigante, Crystal, Gilbert, Amy T., Chipman, Richard B., Kirby, Jordona D., Condori, Rene Edgar, Mills, Samual, Hartley, Chelsea, Forbes, John, Dettinger, Lisa, Dongxiang Xia, Yu Li, vonHoldt, Bridgett

Source: Journal of Wildlife Diseases; Jul2024, Vol. 60 Issue 3, p745-752, 8p

Enhanced Surveillance for Raccoon Rabies Virus Variant and Vaccination of Wildlife for Management -- Omaha, Nebraska, October 2023-July 2024.

Authors: Stein, Sydney R., Beron, Andrew J., Nelson, Kathleen M., Price, Emma, Rodriguez, Sergio E., Shelus, Victoria, Carpenter, Ann, Hess, Amy R., Boutelle, Cassandra, Morgan, Clint N., Gigante, Crystal M., Hutson, Christina L., Loy, John D., Loy, Duan S., Wetzel, Chad, Frederick, Justin, Huse, Lindsay, Orciari, Lillian, Chipman, Richard B., Wallace, Ryan M.

Source: MMWR: Morbidity & Mortality Weekly Report; 10/17/2024, Vol. 73 Issue 41, p933-935, 3p

Subject Terms: PUBLIC health, VIRUS diseases, HEALTH management, RABIES

Geographic Terms: NEBRASKA

How the Orthodox Features of Orthopoxviruses Led to an Unorthodox Mpox Outbreak: What We've Learned, and What We Still Need to Understand.

Authors: Brooks, John T, Reynolds, Mary G, Torrone, Elizabeth, McCollum, Andrea, Spicknall, Ian H, Gigante, Crystal M, Li, Yu, Satheshkumar, Panayampalli S, Quilter, Laura A S, Rao, Agam K, O'Shea, Jesse, Guagliardo, Sarah Anne J, Townsend, Michael, Hutson, Christina L

Source: Journal of Infectious Diseases; 2024 Supplement, Vol. 229, pS121-S131, 11p

Subject Terms: MONKEYPOX, ORTHOPOXVIRUSES

Geographic Terms: AFRICA

How did the 2022 global mpox outbreak happen? A travel-associated case 6 months earlier may provide important clues

Authors: Kreuze, Molly A., Minhaj, Faisal S., Duwell, Monique, Gigante, Crystal M., Kim, Alexander M., Crum, David, Perlmutter, Rebecca, Rubin, Jamie H., Myers, Robert, Lukula, Salimatu L., Ravi-Caldwell, Nivedita, Sockwell, Denise, Chen, Tai-Ho, de Perio, Marie A., Hughes, Christine M., Davidson, Whitni B., Wilkins, Kim, Baird, Nicolle, Lowe, David, Li, Yu, McCollum, Andrea M., Blythe, David, Rao, Agam K.

Source: In Travel Medicine and Infectious Disease September-October 2023 55

Notes from the Field: Emergence of an Mpox Cluster Primarily Affecting Persons Previously Vaccinated Against Mpox — Chicago, Illinois, March 18–June 12, 2023

Authors: Faherty, Emily A.G., Holly, Taylor, Ogale, Yasmin P., Crisler, Gordon, Becht, Ashley, Kern, David, Nicolae, Lavinia, Spencer, Hillary, Wasz, Michael, Kerins, Janna L., Kittner, Alyse, Staton, Amber, Hardnett, Carla, Hutson, Christina, Gigante, Crystal M., Quilter, Laura, Kracalik, Ian, Black, Stephanie, McCollum, Andrea M., Rao, Agam K., Tabidze, Irina

Source: In American Journal of Transplantation August 2023 23(8):1268-1270

Clade II Mpox Surveillance Update -- United States, October 2023-April 2024.

Authors: Tuttle, Alexandra, Hughes, Christine M., Dvorak, Mitchell, Aeschleman, Leah, Davidson, Whitni, Wilkins, Kimberly, Gigante, Crystal, Satheshkumar, Panayampalli S., Rao, Agam K., Minhaj, Faisal S., Christensen, Bryan E., McQuiston, Jennifer H., Hutson, Christina L., McCollum, Andrea M.

Source: MMWR: Morbidity & Mortality Weekly Report; 5/23/2024, Vol. 73 Issue 20, p474-476, 3p

Subject Terms: VIRUSES, HOSPITAL care, VACCINATION, DISEASE outbreaks

Geographic Terms: UNITED States

Fatal Human Rabies Infection With Suspected Host-Mediated Failure of Post-Exposure Prophylaxis Following a Recognized Zoonotic Exposure—Minnesota, 2021.

Authors: Holzbauer, Stacy M, Schrodt, Caroline A, Prabhu, Rajesh M, Asch-Kendrick, Rebecca J, Ireland, Malia, Klumb, Carrie, Firestone, Melanie J, Liu, Gongping, Harry, Katie, Ritter, Jana M, Levine, Min Z, Orciari, Lillian A, Wilkins, Kimberly, Yager, Pamela, Gigante, Crystal M, Ellison, James A, Zhao, Hui, Niezgoda, Michael, Li, Yu, Levis, Robin

Source: Clinical Infectious Diseases; 10/15/2023, Vol. 77 Issue 8, p1201-1208, 8p

Subject Terms: RABIES prevention, BREAKTHROUGH infections, ADENOCARCINOMA, RABIES, BITES & stings, VACCINES, SEQUENCE analysis, IMMUNOGLOBULINS, AUTOPSY, BATS, IMMUNOCOMPROMISED patients, ZOONOSES, RABIES vaccines, TREATMENT effectiveness, RISK assessment, GENOMES, RESEARCH funding, MENINGOENCEPHALITIS, PROSTATE tumors, DISEASE risk factors

Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics.

Authors: Gigante, Crystal M.¹, Dettinger, Lisa², Powell, James W.³, Seiders, Melanie², Condori, Rene Edgar Condori¹, Griesser, Richard³, Okogi, Kenneth⁴, Carlos, Maria⁴, Pesko, Kendra⁵, Breckenridge, Mike⁵, Simon, Edson Michael M.⁶, Chu, Maria Yna Joyce V.⁶, Davis, April D.⁷, Brunt, Scott J.⁷, Orciari, Lillian¹, Yager, Pamela¹, Carson, William C.¹, Hartloge, Claire¹, Saliki, Jeremiah T.⁸, Sanchez, Susan⁸

Source: PLoS ONE. 5/16/2018, Vol. 13 Issue 5, p1-25. 25p.

Subject Terms: *RABIES diagnosis, *LYSSAVIRUS, *POLYMERASE chain reaction, *FLUORESCENT antibody technique, *BIOLOGICAL assay