Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis.

Authors: Cheng, Yiqiu^1,2 (AUTHOR), Zhang, Lei^1,2 (AUTHOR), Ke, Yongping^1,2 (AUTHOR), Dang, Xiaofei¹ (AUTHOR), Miki, Daisuke^1,3 (AUTHOR) daisukemiki@cemps.ac.cn

Source: Scientific Reports. 12/28/2024, Vol. 14 Issue 1, p1-9. 9p.

Subject Terms: *GENOME editing, *GENE targeting, *ARABIDOPSIS thaliana, *PHANEROGAMS, *CRISPRS

Development of a Multiplex RT–qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus.

Authors: Tao, Chunhao¹ (AUTHOR) chunhao_tao@163.com, Zhu, Xizhou² (AUTHOR) zhu.xizhou@hotmail.com, Huang, Ying³ (AUTHOR) hy811cysbm@163.com, Yuan, Weifeng¹ (AUTHOR), Wang, Zhen¹ (AUTHOR), Zhu, Hongfei¹ (AUTHOR), Jia, Hong¹ (AUTHOR) jiahong@caas.cn

Source: International Journal of Molecular Sciences. Dec2024, Vol. 25 Issue 23, p13203. 13p.

Subject Terms: *PORCINE reproductive & respiratory syndrome, *GENE targeting, *DETECTION limit, *RESPIRATORY diseases, *PIGLETS

Quantification of adulterated fox-derived components in meat products by drop digital polymerase chain reaction.

Authors: Wang, Hui¹ (AUTHOR), Chen, Chen¹ (AUTHOR), Zhang, Yan¹ (AUTHOR), Chen, Boxu¹ (AUTHOR), Li, Yongyan¹ (AUTHOR), Jia, Wenshen² (AUTHOR), Chen, Jia³ (AUTHOR) chenjia0311@aliyun.com, Zhou, Wei¹ (AUTHOR) zhouwei0311@163.com

Source: CyTA: Journal of Food. 2024, Vol. 22 Issue 1, p1-7. 7p.

Subject Terms: *POLYMERASE chain reaction, *GENE targeting, *MEAT, *ADULTERATIONS, *DNA

Advancing CRISPR-Based Solutions for COVID-19 Diagnosis and Therapeutics.

Authors: Hadi, Roaa^1,2 (AUTHOR) roaahadi@uark.edu, Poddar, Abhishek^3,4 (AUTHOR) poddar@molbio.mgh.harvard.edu, Sonnaila, Shivakumar⁵ (AUTHOR) sksonnai@uark.edu, Bhavaraju, Venkata Suryanarayana Murthy⁶ (AUTHOR) murthy.1809@gmail.com, Agrawal, Shilpi⁷ (AUTHOR) sagrawal@uark.edu

Source: Cells (2073-4409). Nov2024, Vol. 13 Issue 21, p1794. 24p.

Subject Terms: *VIRUS diseases, *GENE targeting, *CRISPRS, *COVID-19 pandemic, *COVID-19 testing

Non-electrophilic NRF2 activators promote wound healing in human keratinocytes and diabetic mice and demonstrate selective downstream gene targeting.

Authors: Barakat, May^1,2 (AUTHOR), Han, Chen^1,2 (AUTHOR), Chen, Lin¹ (AUTHOR), David, Brian P.³ (AUTHOR), Shi, Junhe^1,4 (AUTHOR), Xu, Angela¹ (AUTHOR), Skowron, Kornelia J.³ (AUTHOR), Johnson, Tatum³ (AUTHOR), Woods, Reginald A.^1,2 (AUTHOR), Ankireddy, Aparna⁵ (AUTHOR), Reddy, Sekhar P.^5,6 (AUTHOR), Moore, Terry W.^3,6 (AUTHOR) twmoore@uic.edu, DiPietro, Luisa A.¹ (AUTHOR) ldipiet@uic.edu

Source: Scientific Reports. 10/24/2024, Vol. 14 Issue 1, p1-24. 24p.

Subject Terms: *TRANSCRIPTION factors, *GENE targeting, *RNA sequencing, *NUCLEAR factor E2 related factor, *CELL migration, *WOUND healing, *SKIN regeneration, KERATINOCYTE differentiation

Preparation and Application of Polyclonal Antibodies for the Rapid Detection of Actinidia Chlorotic Ringspot-Associated Virus.

Authors: Shang, Jing¹ (AUTHOR) shangjing@sicau.edu.cn, Feng, Hongping¹ (AUTHOR), Wang, Yuxuan¹ (AUTHOR) hongpingfenghappy@163.com, Wang, Yunan¹ (AUTHOR), Zhang, Xiao¹ (AUTHOR), Zhang, Zhouyu¹ (AUTHOR)

Source: Viruses (1999-4915). Oct2024, Vol. 16 Issue 10, p1600. 18p.

Subject Terms: *GENE targeting, *ACTINIDIA, *TEST methods, *SENSITIVITY & specificity (Statistics), *IMMUNOGLOBULINS, *KIWIFRUIT

Full-length target sequences of GeoMx digital spatial profiling probes reveal that gene-promiscuity predicts probe sensitivity to EDTA tissue decalcification.

Authors: Oszwald, André¹ (AUTHOR), Zisser, Lucia² (AUTHOR), Schachner, Helga¹ (AUTHOR), Kaltenecker, Christopher¹ (AUTHOR), Wasinger, Gabriel¹ (AUTHOR), Rohrbeck, Johannes¹ (AUTHOR), Kozakowsky, Nicolas¹ (AUTHOR), Tiefenbacher, Andreas¹ (AUTHOR), Rees, Andrew J.¹ (AUTHOR), Kain, Renate¹ (AUTHOR) renate.kain@meduniwien.ac.at

Source: Scientific Reports. 9/10/2024, Vol. 14 Issue 1, p1-8. 8p.

Subject Terms: *TRANSCRIPTOMES, *GENE targeting, *TISSUES, *HUMAN beings

Adding metabolic tasks to human GEM models to improve the study of gene targets and their associated toxicities.

Authors: Guil, Francisco¹ (AUTHOR) fguil@um.es, García, Raquel² (AUTHOR), García, José M.¹ (AUTHOR)

Source: Scientific Reports. 7/27/2024, Vol. 14 Issue 1, p1-9. 9p.

Subject Terms: *GENE targeting, *CANCER cell proliferation, *BIOMASS production, *METABOLIC models, *DRUG toxicity

In utero adeno-associated virus (AAV)-mediated gene delivery targeting sensory and supporting cells in the embryonic mouse inner ear.

Authors: Barbosa Spinola, Carla Maria^1,2 (AUTHOR), Boutet de Monvel, Jacques¹ (AUTHOR), Safieddine, Saaid^1,3 (AUTHOR), Lahlou, Ghizlène^1,4 (AUTHOR) ghizlene.lahlou@pasteur.fr, Etournay, Raphaël^1,2 (AUTHOR) ghizlene.lahlou@pasteur.fr

Source: PLoS ONE. 7/19/2024, Vol. 19 Issue 7, p1-16. 16p.

Subject Terms: *INNER ear, *GENE targeting, *ADENO-associated virus, *DEVELOPMENTAL biology, *VIRAL tropism, *SENSORINEURAL hearing loss, *HAIR cells

Cas12a-mediated gene targeting by sequential transformation strategy in Arabidopsis thaliana.

Authors: Li, Jing^1,2 (AUTHOR), Wei, Qi^1,2 (AUTHOR), Cheng, Yiqiu^1,2 (AUTHOR), Kong, Dali^1,2 (AUTHOR), Kong, Zhe^1,2 (AUTHOR), Ke, Yongping^1,2 (AUTHOR), Dang, Xiaofei¹ (AUTHOR), Zhu, Jian-Kang³ (AUTHOR), Shimada, Hiroaki⁴ (AUTHOR), Miki, Daisuke¹ (AUTHOR) daisukemiki@cemps.ac.cn

Source: BMC Plant Biology. 7/12/2024, Vol. 24 Issue 1, p1-15. 15p.

Subject Terms: *GENE targeting, *SECOND harmonic generation, *PHANEROGAMS, *GENOME editing, *NUCLEASES

Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing.

Authors: Kiyokawa, Kazuya^1,2, Sakuma, Tetsushi³, Moriguchi, Kazuki¹, Sugiyama, Minetaka⁴, Akao, Takeshi⁵, Yamamoto, Takashi^2,3, Suzuki, Katsunori¹, ksuzuki@hiroshima-u.ac.jp

Source: Applied Microbiology & Biotechnology; 7/12/2024, Vol. 108 Issue 1, p1-17, 17p

A G-quadruplex-binding platinum complex induces cancer mitochondrial dysfunction through dual-targeting mitochondrial and nuclear G4 enriched genome.

Authors: Kuang, Keli¹ (AUTHOR), Li, Chunyan¹ (AUTHOR), Maksut, Fatlinda^2,3 (AUTHOR), Ghosh, Deepanjan^2,3 (AUTHOR), Vinck, Robin⁴ (AUTHOR), Wang, Maolin¹ (AUTHOR), Poupon, Joël⁵ (AUTHOR), Xiang, Run⁶ (AUTHOR), Li, Wen⁷ (AUTHOR), Li, Fei¹ (AUTHOR), Wang, Zhu¹ (AUTHOR), Du, Junrong¹ (AUTHOR), Teulade-Fichou, Marie-Paule^2,3 (AUTHOR), Gasser, Gilles⁴ (AUTHOR), Bombard, Sophie^2,3 (AUTHOR) Sophie.bombard@curie.fr, Jia, Tao^1,2,3 (AUTHOR) taojia86@scu.edu.cn

Source: Journal of Biomedical Science. 5/13/2024, Vol. 31 Issue 1, p1-21. 21p.

Subject Terms: *QUADRUPLEX nucleic acids, *GENE targeting, *PLATINUM, *GENOMES, *MITOCHONDRIAL DNA, *MITOCHONDRIA, *DNA structure, *REACTIVE oxygen species

Repeat mediated excision of gene drive elements for restoring wild-type populations.

Authors: Chennuri, Pratima R.¹, Zapletal, Josef², Monfardini, Raquel D.¹, Ndeffo-Mbah, Martial Loth^3,4, Adelman, Zach N.¹, Myles, Kevin M.¹, mylesk@tamu.edu

Source: PLoS Genetics; 11/7/2024, Vol. 20 Issue 11, p1-28, 28p

Cooperative Path Planning of Multiple Unmanned Aerial Vehicles Using Cylinder Vector Particle Swarm Optimization With Gene Targeting

Authors: Huang, C., Ma, H., Zhou, X., Deng, W.

Source: IEEE Sensors Journal IEEE Sensors J. Sensors Journal, IEEE. 25(5):8470-8480 Mar, 2025

Gene targeting in adult organs using in vivo cleavable donor plasmids for CRISPR-Cas9 and CRISPR-Cas12a.

Authors: Ishibashi, Riki^1,2 (AUTHOR) rishibas@infront.kyoto-u.ac.jp, Maki, Ritsuko¹ (AUTHOR), Toyoshima, Fumiko^1,2,3 (AUTHOR)

Source: Scientific Reports. 3/31/2024, Vol. 14 Issue 1, p1-11. 11p.

Subject Terms: *GENE targeting, *CRISPRS, *GENOME editing, *PLASMIDS, *GENE therapy, *ADULTS

Development and Evaluation of an In-House Real-Time RT-PCR Targeting nsp10 Gene for SARS-CoV-2 Detection.

Authors: Yip, Cyril Chik-Yan¹ (AUTHOR) vcccheng@hku.hk, Poon, Jane Hau-Ching² (AUTHOR) janepn@hku.hk, Leung, Kit-Hang² (AUTHOR) kithang55@gmail.com, Chan, Wan-Mui² (AUTHOR) mbally@hku.hk, Ip, Jonathan Daniel² (AUTHOR) jdip1007@connect.hku.hk, Chu, Allen Wing-Ho² (AUTHOR) awhchu@hku.hk, Cheng, Vincent Chi-Chung¹ (AUTHOR), Yuen, Kwok-Yung² (AUTHOR) kyyuen@hku.hk, To, Kelvin Kai-Wang² (AUTHOR) kelvinto@hku.hk

Source: International Journal of Molecular Sciences. Mar2024, Vol. 25 Issue 6, p3552. 11p.

Subject Terms: *GENE targeting, *SARS-CoV-2, *OPIOID receptors, *SARS-CoV-2 Omicron variant, *DETECTION limit, *SENSITIVITY & specificity (Statistics), *POLYMERASE chain reaction, *REVERSE transcriptase polymerase chain reaction

Identification of Key Molecular Pathways and Associated Genes as Targets to Overcome Radiotherapy Resistance Using a Combination of Radiotherapy and Immunotherapy in Glioma Patients.

Authors: Zhang, Tianqi¹ (AUTHOR) tianqi.zhang@nyulangone.org, Zhang, Qiao¹ (AUTHOR) qiao.zhang@nyulangone.org, He, Xinwei² (AUTHOR) hexw26@mail.sysu.edu.cn, Lu, Yuting¹ (AUTHOR) yl4036@nyu.edu, Shao, Andrew³ (AUTHOR) as13381@nyu.edu, Sun, Xiaoqiang² (AUTHOR) sunxq6@mail.sysu.edu.cn, Shao, Yongzhao¹ (AUTHOR) sunxq6@mail.sysu.edu.cn

Source: International Journal of Molecular Sciences. Mar2024, Vol. 25 Issue 5, p3076. 18p.

Subject Terms: *GLIOMAS, *GENE targeting, *HIPPO signaling pathway, *GENETIC models, *PHOSPHATIDYLINOSITOL 3-kinases

A target enrichment approach for enhanced recovery of Synchytrium endobioticum nuclear genome sequences.

Authors: Nguyen, Hai D. T.¹ (AUTHOR) hai.nguyen2@agr.gc.ca, Ponomareva, Ekaterina¹ (AUTHOR), Dadej, Kasia¹ (AUTHOR), Smith, Donna² (AUTHOR), Antoun, Melissa² (AUTHOR), van der Lee, Theo A. J.³ (AUTHOR), van de Vossenberg, Bart T. L. H.⁴ (AUTHOR) hai.nguyen2@agr.gc.ca

Source: PLoS ONE. 2/12/2024, Vol. 19 Issue 2, p1-11. 11p.

Subject Terms: *POTATOES, *GENE targeting, *GENOMES, *SEQUENCE analysis, *NUCLEOTIDE sequencing, *WARTS, *SPORES, *TUBERS

SP1 and KROX20 Regulate the Proliferation of Dermal Papilla Cells and Target the CUX1 Gene.

Authors: Lv, Xiaoyang^1,2 (AUTHOR) dx120170085@yzu.edu.cn, He, Mingliang³ (AUTHOR), Zhou, Hui³ (AUTHOR), Wang, Shanhe^2,3 (AUTHOR), Cao, Xiukai^1,2 (AUTHOR) yuanzehu1988@163.com, Yuan, Zehu^1,2 (AUTHOR), Getachew, Tesfaye⁴ (AUTHOR), Li, Yutao⁵ (AUTHOR), Sun, Wei^1,2,3 (AUTHOR) sunwei@yzu.edu.cn

Source: Animals (2076-2615). Feb2024, Vol. 14 Issue 3, p429. 13p.

Subject Terms: *TRANSCRIPTION factor Sp1, *GENE targeting, *REGULATOR genes, *HAIR follicles, *GENETIC transcription regulation, *HOMEOBOX genes

A Comparative Transcriptomic Study and Key Gene Targeting of Lamprey Gonadal Immune Response.

Authors: Xu, Xiangting^1,2,3 (AUTHOR), Xu, Jing^1,2,3,4 (AUTHOR), Qiu, Mingyue^1,2,3 (AUTHOR), Yu, Yang^1,2,3,5 (AUTHOR), Gou, Meng^1,2,3 (AUTHOR), Pang, Yue^1,2,3 (AUTHOR), Li, Qingwei^1,2,3 (AUTHOR) liqw@263.net, Su, Peng^1,2,3 (AUTHOR) sp4046@163.com

Source: Immunological Investigations. Feb2024, Vol. 53 Issue 2, p241-260. 20p.

Subject Terms: *GENE targeting, *IMMUNE response, *LAMPREYS, *IMMUNOSTAINING, *GONADS