CRISPR/Cas12a-mediated gene silencing across diverse functional genes demonstrates single gene-specific spacer efficacy in Mycobacterium smegmatis.

Authors: Sodani, Megha^1,2 (AUTHOR), Misra, Chitra S.³ (AUTHOR), Kulkarni, Savita^1,2 (AUTHOR) savita.kulkarni1@gmail.com, Rath, Devashish^2,3 (AUTHOR) devrath@barc.gov.in

Source: Journal of Biological Engineering. 2/28/2025, Vol. 19 Issue 1, p1-14. 14p.

Subject Terms: *GENE libraries, *MYCOBACTERIUM smegmatis, *LIFE sciences, *FUNCTIONAL genomics, *DNA probes, *MYCOBACTERIUM tuberculosis

JNK Inhibition Overcomes Resistance of Metastatic Tetraploid Cancer Cells to Irradiation-Induced Apoptosis.

Authors: Jemaà, Mohamed^1,2,3 (AUTHOR) mohamed.jemaa@fmt.utm.tn, Setti Boubaker, Nouha^4,5 (AUTHOR) nouha.setti@hotmail.fr, Kerkeni, Nesrine¹ (AUTHOR), M. Huber, Stephan⁶ (AUTHOR) mohamed.jemaa@fmt.utm.tn

Source: International Journal of Molecular Sciences. Feb2025, Vol. 26 Issue 3, p1209. 13p.

Subject Terms: *MEMBRANE potential, *GENE libraries, *CELL cycle, *COLON cancer, *CHROMOSOMES

The Prospect of Gene Exploitation through Soil Metagenomics.

Authors: Sagar, Kalpana¹, Priti, Km.¹, Gupta, Kartikey Kumar¹, Katara, Annapurna¹, Yadav, Archana², Chandra, Harish¹ hreesh5@gmail.com

Source: Journal of Pure & Applied Microbiology. Dec2024, Vol. 18 Issue 4, p2228-2243. 16p.

Subject Terms: *BIOTECHNOLOGY, *GENE libraries, *METAGENOMICS, *BACTERIAL physiology, *MICROBIAL diversity

A CRISPR/Cas9‐mediated mutant library of seed‐preferred genes in rice.

Authors: Zhao, Dongsheng^1,2 (AUTHOR), Chen, Siyu¹ (AUTHOR), Han, Yangshuo¹ (AUTHOR), Liu, Guanqing¹ (AUTHOR), Liu, Jinyu¹ (AUTHOR), Yang, Qingqing¹ (AUTHOR), Zhang, Tao^1,2 (AUTHOR) zhangtao@yzu.edu.cn, Shen, Jilei¹ (AUTHOR), Fan, Xiaolei¹ (AUTHOR), Zhang, Changquan^1,2 (AUTHOR), Li, Qianfeng^1,2 (AUTHOR) qfli@yzu.edu.cn, Chen, Chen^1,2 (AUTHOR) chenchen@yzu.edu.cn, Liu, Qiaoquan^1,2 (AUTHOR) qqliu@yzu.edu.cn

Source: Plant Biotechnology Journal. Nov2024, Vol. 22 Issue 11, p3012-3014. 3p.

Subject Terms: *GENE expression, *GENE libraries, *REPORTER genes, *GERMPLASM, *FRAMESHIFT mutation

Characteristics and Comparative Analysis of Mitochondrial Genomes of the Aphid Genus Hyalopterus Koch (Hemiptera: Aphididae: Aphidinae).

Authors: Zhang, Xiaolu^1,2 (AUTHOR) zhangxiaolu@ioz.ac.cn, Li, Cailing^1,2 (AUTHOR) licailing@ioz.ac.cn, Jiang, Liyun¹ (AUTHOR) jiangliyun@ioz.ac.cn, Qiao, Gexia^1,2 (AUTHOR) qiaogx@ioz.ac.cn, Chen, Jing¹ (AUTHOR) qiaogx@ioz.ac.cn

Source: Insects (2075-4450). Jun2024, Vol. 15 Issue 6, p389. 13p.

Subject Terms: *HEMIPTERA, *GENE libraries, *APHIDS, *TANDEM repeats, *MITOCHONDRIA, *STONE fruit, *GENOMES, *COMPARATIVE genomics

Population Genomics and Conservation of Copernicia alba: a Non-Timber Palm of Economic Importance in the Brazilian Chaco.

Authors: Costa, Marcones Ferreira^1,2 (AUTHOR) marconescosta@ufpi.edu.br, Marroquín, Jonathan Andre Morales² (AUTHOR), de Araújo Batista, Carlos Eduardo³ (AUTHOR), Alves-Pereira, Alessandro² (AUTHOR), de Almeida Vieira, Fábio⁴ (AUTHOR), Zucchi, Maria Imaculada^2,3,5 (AUTHOR)

Source: Plant Molecular Biology Reporter. Jun2024, Vol. 42 Issue 2, p235-245. 11p.

Subject Terms: *GENE libraries, *GENETIC variation, *POPULATION differentiation, *TROPICAL dry forests, *INBREEDING, *GENOMICS

Geographic Terms: GRAN Chaco

Development of genomic and genetic resources facilitating molecular genetic studies on untapped Myanmar rice germplasms.

Authors: Tomoyuki Furuta¹, Ohm Mar Saw², Sandar Moe², Khin Thanda Win³, Moe Moe Hlaing¹, Aye Lae Lae Hlaing², Min San Thein², Hideshi Yasui³, Motoyuki Ashikari⁴, Atsushi Yoshimura³, Yoshiyuki Yamagata³ yoshiyuk@agr.kyushu-u.ac.jp

Source: Breeding Science. 2024, Vol. 72 Issue 2, p124-137. 14p.

Subject Terms: *GENE libraries, *GENOME-wide association studies, *GERMPLASM, *GENETIC variation, *CLIMATE change

Geographic Terms: MYANMAR

Massive integration of large gene libraries in the chromosome of Escherichia coli.

Authors: Cerdán, Lidia¹ (AUTHOR), Álvarez, Beatriz¹ (AUTHOR), Fernández, Luis Ángel¹ (AUTHOR) lafdez@cnb.csic.es

Source: Microbial Biotechnology. Jan2024, Vol. 17 Issue 1, p1-16. 16p.

Subject Terms: *GENE libraries, *PLASMIDS, *CHROMOSOMES, *EPIDERMAL growth factor receptors, *BACTERIAL chromosomes, *ESCHERICHIA coli, *CHROMOSOME replication

RLB-MALBAC: a rapid, non-transposase method for synthesizing unbiased sequencing libraries from low amount of genomic DNA for Nanopore sequencing.

Authors: Gadkar, Vijay J.^1,2 (AUTHOR) vijay.gadkar@cw.bc.ca, Goldfarb, David M.^1,2 (AUTHOR), Dhaliwal, Sukh^1,2 (AUTHOR), Gubbay, Jonathan B.^1,2 (AUTHOR), Tilley, Peter A. G.^1,2 (AUTHOR)

Source: BMC Methods. 3/3/2025, Vol. 2 Issue 1, p1-17. 17p.

Subject Terms: *GENE libraries, *BACTERIOPHAGE lambda, *LIFE sciences, *VIRAL genomes, *DRUG resistance in bacteria

Recent technology advancements in large‐scale DNA assembly.

Authors: Li, Jielin^1,2 (AUTHOR), Cheng, Li¹ (AUTHOR), Ma, Yingxin¹ (AUTHOR), Cai, Yizhi^1,3 (AUTHOR), Dai, Junbiao^1,2,4 (AUTHOR) daijunbiao@caas.cn

Source: Quantitative Biology. Mar2025, Vol. 13 Issue 1, p1-9. 9p.

Subject Terms: *GENE expression, *GENE libraries, *BACTERIAL artificial chromosomes, *ARTIFICIAL chromosomes, *GENETIC engineering, *SYNTHETIC biology

Multi-omics analysis of SIV-specific CD8⁺ T cells in multiple anatomical sites.

Authors: Simpson, Jennifer¹, Dulek, Brittany², Schaughency, Paul², Brenchley, Jason M.¹, jbrenchl@niaid.nih.gov

Source: PLoS Pathogens; 9/9/2024, Vol. 20 Issue 9, p1-23, 23p

Metagenomic profiling pipelines improve taxonomic classification for 16S amplicon sequencing data.

Authors: Odom, Aubrey R.^1,2 (AUTHOR), Faits, Tyler^1,2 (AUTHOR), Castro-Nallar, Eduardo^3,4 (AUTHOR), Crandall, Keith A.⁵ (AUTHOR), Johnson, W. Evan⁶ (AUTHOR) w.evan.johnson@rutgers.edu

Source: Scientific Reports. 8/26/2023, Vol. 13 Issue 1, p1-12. 12p.

Subject Terms: *SHOTGUN sequencing, *GENE libraries, *WHOLE genome sequencing, *METAGENOMICS, *BACTERIAL communities, *SPECIES diversity

Expression of Hairpin-Enriched Mitochondrial DNA in Two Hairworm Species (Nematomorpha).

Authors: Nikolaeva, Olga V.¹ (AUTHOR), Beregova, Aleksandra M.^1,2 (AUTHOR), Efeykin, Boris D.^3,4 (AUTHOR) s_e_spiridonov@rambler.ru, Miroliubova, Tatiana S.³ (AUTHOR), Zhuravlev, Andrey Yu.⁵ (AUTHOR), Ivantsov, Andrey Yu.⁶ (AUTHOR), Mikhailov, Kirill V.^1,4 (AUTHOR), Spiridonov, Sergei E.³ (AUTHOR), Aleoshin, Vladimir V.^1,4 (AUTHOR) aleshin@genebee.msu.su

Source: International Journal of Molecular Sciences. Jul2023, Vol. 24 Issue 14, p11411. 17p.

Subject Terms: *GENE libraries, *LIFE cycles (Biology), *SPECIES, *CHLOROPLAST DNA, *GENOMES, *PLANT mitochondria, *MITOCHONDRIAL DNA, *MITOCHONDRIA

Bacterial Communities of the Selenga River, Its Tributaries, and the Selenga Shallows in the Summer of 2021.

Authors: Zakharenko, A. S.¹ (AUTHOR) zakharenko@lin.irk.ru, Tomberg, I. V.¹ (AUTHOR), Bukin, S. V.¹ (AUTHOR), Blinov, V. V.¹ (AUTHOR), Zemskaya, T. I.¹ (AUTHOR)

Source: Microbiology (00262617). Dec2024, Vol. 93 Issue 6, p891-903. 13p.

Subject Terms: *GENE libraries, *BACTERIAL diversity, *WATER quality, *CHLORIDE ions, *RIBOSOMAL RNA

Fast and precise multi-site mutagenesis on linear DNA fragments.

Authors: Zhang, Jie^1,2 (AUTHOR), Yang, Youhui^1,2 (AUTHOR), Wang, Zhaoguan^1,2 (AUTHOR), Liu, Qian^1,2 (AUTHOR), Li, Peixian^1,2 (AUTHOR), Kang, Shaohua^1,2 (AUTHOR), Qi, Hao^1,2 (AUTHOR) haoq@tju.edu.cn

Source: Biotechnology & Biotechnological Equipment. Dec2024, Vol. 38 Issue 1, p1-8. 8p.

Subject Terms: *DNA denaturation, *GENE libraries, *GREEN fluorescent protein, *SINGLE-stranded DNA, *SITE-specific mutagenesis

Mrc1^Claspin is essential for heterochromatin maintenance in Schizosaccharomyces pombe.

Authors: Kawakami, Kei^1,2,3 (AUTHOR) keikawa@med.shimane-u.ac.jp, Ueno, Yukari¹ (AUTHOR), Hayama, Nao¹ (AUTHOR), Tanaka, Katsunori^1,2 (AUTHOR) katsunori@kwansei.ac.jp

Source: Genes to Cells. Dec2024, Vol. 29 Issue 12, p1207-1224. 18p.

Subject Terms: *SCHIZOSACCHAROMYCES, *GENE libraries, *SCHIZOSACCHAROMYCES pombe, *HETEROCHROMATIN, *DNA replication

Exploring the Potency of Lower Respiratory Tract Microbiome as Biomarkers for Lung Cancer.

Alternate Title: استكشاف فاعلية مايكروبيوم الجهاز التنفسي السفلي كمؤشرات حيوية لسرطان الرئة.

Authors: Rasheed, Sama Kamal¹ sama.kamal9977@gmail.com, Kadhim Al-Khafaji, Ahmed Salim¹

Source: Iraqi Journal of Science. 2024, Vol. 65 Issue 11, p6336-6352. 17p.

Subject Terms: *HAEMOPHILUS influenzae, *GENE libraries, *LUNG cancer, *HUMAN microbiota, *DISEASE risk factors

Optimized genome-wide CRISPR screening enables rapid engineering of growth-based phenotypes in Yarrowia lipolytica.

Authors: Robertson, Nicholas R.¹ (AUTHOR), Trivedi, Varun² (AUTHOR), Lupish, Brian¹ (AUTHOR), Ramesh, Adithya² (AUTHOR), Aguilar, Yuna¹ (AUTHOR), Carrera, Stephanie² (AUTHOR), Lee, Sangcheon² (AUTHOR), Arteaga, Anthony³ (AUTHOR), Nguyen, Alexander² (AUTHOR), Lenert-Mondou, Chase⁴ (AUTHOR), Harland-Dunaway, Marcus⁵ (AUTHOR), Jinkerson, Robert² (AUTHOR), Wheeldon, Ian^1,2,3 (AUTHOR) wheeldon@ucr.edu

Source: Metabolic Engineering. Nov2024, Vol. 86, p55-65. 11p.

Subject Terms: *GENE libraries, *PHENOTYPES, *HYDROCARBON analysis, *GENE knockout, *FATTY acids

Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification.

Authors: Manojlovic, Zarko¹ (AUTHOR), Wlodarczyk, Jordan² (AUTHOR), Okitsu, Cindy³ (AUTHOR), Jin, Yuxin⁴ (AUTHOR), Van Den Berg, David⁵ (AUTHOR), Lieber, Michael R.³ (AUTHOR), Hsieh, Chih-Lin¹ (AUTHOR) hsieh_c@med.usc.edu

Source: BMC Research Notes. 4/27/2023, Vol. 16 Issue 1, p1-6. 6p.

Subject Terms: *GENE libraries, *COLON (Anatomy), *HUMAN genome, *NUCLEOTIDE sequencing, *DNA

Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in Stenotrophomonas maltophilia and Chryseobacterium spp. Bacteria of Soil Origin.

Authors: Klimkaitė, Laurita¹ (AUTHOR) laurita.klimkaite@kc.vu.lt, Ragaišis, Ignas¹ (AUTHOR) ignasraga@gmail.com, Krasauskas, Renatas¹ (AUTHOR) renatas.krasauskas@gf.vu.lt, Ružauskas, Modestas² (AUTHOR) modestas.ruzauskas@lsmuni.lt, Sužiedėlienė, Edita¹ (AUTHOR) edita.suziedeliene@cr.vu.lt, Armalytė, Julija¹ (AUTHOR) julija.armalyte@gf.vu.lt

Source: International Journal of Molecular Sciences. Apr2023, Vol. 24 Issue 7, p6037. 15p.

Subject Terms: *GENE libraries, *STENOTROPHOMONAS maltophilia, *DRUG resistance in bacteria, *SOIL microbiology, *AGRICULTURE

Geographic Terms: LITHUANIA