Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay

Authors: Bastian Breiner, Daniel M. Kainz, Stefan Wagner, Maxime Gavage, Serhat Sahakalkan, Riccardo Marega, Felix von Stetten, Anna Klebes

Source: Proceedings, Vol 97, Iss 1, p 199 (2024)

Subject Terms: lateral flow assay, centrifugal microfluidics, sample preparation, allergen detection, General Works

File Description: electronic resource

Relation: https://www.mdpi.com/2504-3900/97/1/199; https://doaj.org/toc/2504-3900

Access URL: https://doaj.org/article/00098fa4d4474a7591ec60de5d3791a3

Multianalyte-Compatible Lysis for the Detection of P. aeruginosa and IL-6 via Lateral Flow Immunoassay

Authors: Anna Klebes, Bianka Pfefferle, Anna-Sophia Kittel, Bastian Breiner, Nadine Borst, Felix von Stetten

Source: Proceedings, Vol 97, Iss 1, p 194 (2024)

Subject Terms: wound diagnostics, paper-based multianalyte biosensor, isothermal amplification, General Works

File Description: electronic resource

Relation: https://www.mdpi.com/2504-3900/97/1/194; https://doaj.org/toc/2504-3900

Access URL: https://doaj.org/article/a9949a5e6cd449aa84a08f6f0393fd2e

Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi

Authors: Lisa Becherer, Sascha Knauf, Michael Marks, Simone Lueert, Sieghard Frischmann, Nadine Borst, Felix von Stetten, Sibauk Bieb, Yaw Adu-Sarkodie, Kingsley Asiedu, Oriol Mitjà, Mohammed Bakheit

Source: Emerging Infectious Diseases, Vol 26, Iss 2, Pp 282-288 (2020)

Subject Terms: Yaws, neglected tropical disease, Treponema pallidum, Haemophilus ducreyi, loop-mediated isothermal amplification, LAMP, Medicine, Infectious and parasitic diseases, RC109-216

File Description: electronic resource

Relation: https://wwwnc.cdc.gov/eid/article/26/2/19-0505_article; https://doaj.org/toc/1080-6040; https://doaj.org/toc/1080-6059

Access URL: https://doaj.org/article/df90fd86451c4369acc0f48212e51e1d

Analyzing siRNA Concentration, Complexation and Stability in Cationic Dendriplexes by Stem-Loop Reverse Transcription-qPCR

Authors: Maximilian Neugebauer, Clara E. Grundmann, Michael Lehnert, Felix von Stetten, Susanna M. Früh, Regine Süss

Source: Pharmaceutics, Vol 14, Iss 7, p 1348 (2022)

Subject Terms: reverse transcription real-time polymerase chain reaction (RT-qPCR), stem-loop primer, small interfering RNA (siRNA), drug delivery, dendriplexes, nitrogen-to-phosphate (N/P) ratio, Pharmacy and materia medica, RS1-441

File Description: electronic resource

Relation: https://www.mdpi.com/1999-4923/14/7/1348; https://doaj.org/toc/1999-4923

Access URL: https://doaj.org/article/3476219801744b6681a1dbe1d841e74d

Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification

Authors: Yu-Ting Kao, Silvia Calabrese, Nadine Borst, Michael Lehnert, Yu-Kai Lai, Franziska Schlenker, Peter Juelg, Roland Zengerle, Piotr Garstecki, Felix von Stetten

Source: Biosensors, Vol 12, Iss 4, p 237 (2022)

Subject Terms: fluorescence in situ hybridization, locked nucleic acid, molecular beacons, droplet microfluidics, amplification-free detection, digital assays, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.mdpi.com/2079-6374/12/4/237; https://doaj.org/toc/2079-6374

Access URL: https://doaj.org/article/9e70d71d2f3147bb814191c5892ebf6b

OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice

Authors: Desirée Baumgartner, Benita Johannsen, Mara Specht, Jan Lüddecke, Markus Rombach, Sebastian Hin, Nils Paust, Felix von Stetten, Roland Zengerle, Christopher Herz, Johannes R. Peham, Pune N. Paqué, Thomas Attin, Joël S. Jenzer, Philipp Körner, Patrick R. Schmidlin, Thomas Thurnheer, Florian J. Wegehaupt, Wendy E. Kaman, Andrew Stubbs, John P. Hays, Viorel Rusu, Alex Michie, Thomas Binsl, David Stejskal, Michal Karpíšek, Kai Bao, Nagihan Bostanci, Georgios N. Belibasakis, Konstantinos Mitsakakis

Source: Biosensors, Vol 11, Iss 11, p 423 (2021)

Subject Terms: dental practice, point-of-care diagnostics, treatment monitoring, oral health, periodontitis, caries, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.mdpi.com/2079-6374/11/11/423; https://doaj.org/toc/2079-6374

Access URL: https://doaj.org/article/99cf5461c1914ad484eb0d8614eb3dc2

Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

Authors: Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten

Source: BioTechniques, Vol 61, Iss 3, Pp 123-128 (2016)

Subject Terms: multiplex PCR, mediator probe PCR, universal reporter, MRSA, food panel, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818

Access URL: https://doaj.org/article/f4d9af57eaa7470fabf45325be843c3f

Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay

Authors: Anna Brunauer, René D. Verboket, Daniel M. Kainz, Felix von Stetten, Susanna M. Früh

Source: Biosensors, Vol 11, Iss 3, p 74 (2021)

Subject Terms: recombinase polymerase amplification, nucleic acid lateral flow immunoassay, point-of-care diagnostics, paper-based detection, nucleic acid amplification test, wound exudate, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.mdpi.com/2079-6374/11/3/74; https://doaj.org/toc/2079-6374

Access URL: https://doaj.org/article/43769b399eb64370a3262ba6e28e4d0a

Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP

Authors: Lisa Becherer, Jacob Friedrich Hess, Sieghard Frischmann, Mohammed Bakheit, Hans Nitschko, Silvina Stinco, Friedrich Zitz, Hannes Hofer, Giampiero Porro, Florian Hausladen, Karl Stock, Dominik Drossart, Holger Wurm, Hanna Kuhn, Dominik Huber, Tobias Hutzenlaub, Nils Paust, Mark Keller, Oliver Strohmeier, Simon Wadle, Nadine Borst, Roland Zengerle, Felix von Stetten

Source: Micromachines, Vol 12, Iss 2, p 159 (2021)

Subject Terms: human T-cell lymphotropic virus 1, digital loop-mediated isothermal amplification, point-of-care testing, centrifugal microfluidics, proviral load measurements, Mechanical engineering and machinery, TJ1-1570

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Relation: https://www.mdpi.com/2072-666X/12/2/159; https://doaj.org/toc/2072-666X

Access URL: https://doaj.org/article/4a5b12acd76d4d2a9e78791e90dfe337

Versatile Tool for Droplet Generation in Standard Reaction Tubes by Centrifugal Step Emulsification

Authors: Martin Schulz, Sophia Probst, Silvia Calabrese, Ana R. Homann, Nadine Borst, Marian Weiss, Felix von Stetten, Roland Zengerle, Nils Paust

Source: Molecules, Vol 25, Iss 8, p 1914 (2020)

Subject Terms: microfluidics, centrifugal step emulsification, droplet generation, emulsification, compartmentation, digital amplification, Organic chemistry, QD241-441

File Description: electronic resource

Relation: https://www.mdpi.com/1420-3049/25/8/1914; https://doaj.org/toc/1420-3049

Access URL: https://doaj.org/article/be136a0e990a485aa5a7b1fe6c16cb87

Centrifugal Step Emulsification can Produce Water in Oil Emulsions with Extremely High Internal Volume Fractions

Authors: Friedrich Schuler, Nils Paust, Roland Zengerle, Felix von Stetten

Source: Micromachines, Vol 6, Iss 8, Pp 1180-1188 (2015)

Subject Terms: droplet, high-throughput, centrifugal step emulsification, step emulsification, centrifugal microfluidics, internal phase ratio, internal volume fraction, high internal phase ratio emulsion (HIPRE), Mechanical engineering and machinery, TJ1-1570

File Description: electronic resource

Relation: http://www.mdpi.com/2072-666X/6/8/1180; https://doaj.org/toc/2072-666X

Access URL: https://doaj.org/article/c87ea2c14c2a452ab74f83c84c33c485

Inkjet-Printing of Nanoparticle Gold and Silver Ink on Cyclic Olefin Copolymer for DNA-Sensing Applications

Authors: Martin Trotter, Daniel Juric, Zahra Bagherian, Nadine Borst, Kerstin Gläser, Thomas Meissner, Felix von Stetten, André Zimmermann

Source: Sensors, Vol 20, Iss 5, p 1333 (2020)

Subject Terms: inkjet-printing, gold nanoparticles, electrode integration, dna sensing, electrochemical sensors, lab-on-a-chip, Chemical technology, TP1-1185

File Description: electronic resource

Relation: https://www.mdpi.com/1424-8220/20/5/1333; https://doaj.org/toc/1424-8220

Access URL: https://doaj.org/article/e4d85d5d33fe4f6698e1018598ba90fa

Real–time stability testing of air–dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan

Authors: Markus Rombach, Dominique Kosse, Bernd Faltin, Simon Wadle, Günter Roth, Roland Zengerle, Felix von Stetten

Source: BioTechniques, Vol 57, Iss 3, Pp 151-155 (2014)

Subject Terms: Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818

Access URL: https://doaj.org/article/5cdbbbccd25649d5886694d7e7c041c1

Active Continuous-Flow Micromixer Using an External Braille Pin Actuator Array

Authors: Junichi Miwa, Roland Zengerle, Felix von Stetten, Yawar Abbas

Source: Micromachines, Vol 4, Iss 1, Pp 80-89 (2013)

Subject Terms: active micromixer, continuous-flow, chaotic advection, channel wall deflection, PDMS chip, cell sample dilution, microfluidic Braille pin actuated platform, Mechanical engineering and machinery, TJ1-1570

File Description: electronic resource

Relation: http://www.mdpi.com/2072-666X/4/1/80; https://doaj.org/toc/2072-666X

Access URL: https://doaj.org/article/eb9ebcaa1d674e66a6a0eda285c93363

Automated Forensic Animal Family Identification by Nested PCR and Melt Curve Analysis on an Off-the-Shelf Thermocycler Augmented with a Centrifugal Microfluidic Disk Segment.

Authors: Mark Keller, Jana Naue, Roland Zengerle, Felix von Stetten, Ulrike Schmidt

Source: PLoS ONE, Vol 10, Iss 7, p e0131845 (2015)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC4492497?pdf=render; https://doaj.org/toc/1932-6203

Access URL: https://doaj.org/article/fd1fa68be7e442e8b0c78a549a40e046

Rapid molecular assays for the detection of yellow fever virus in low-resource settings.

Authors: Camille Escadafal, Oumar Faye, Amadou Alpha Sall, Ousmane Faye, Manfred Weidmann, Oliver Strohmeier, Felix von Stetten, Josef Drexler, Michael Eberhard, Matthias Niedrig, Pranav Patel

Source: PLoS Neglected Tropical Diseases, Vol 8, Iss 3, p e2730 (2014)

Subject Terms: Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270

File Description: electronic resource

Relation: http://europepmc.org/articles/PMC3945292?pdf=render; https://doaj.org/toc/1935-2727; https://doaj.org/toc/1935-2735

Access URL: https://doaj.org/article/775f90d2d3a342a0a8a0d07f78885c95

Continuous microfluidic DNA extraction using phase-transfer magnetophoresis.

Authors: Marc Karle, Junichi Miwa, Gregor Czilwik, Volker Auwärter, Günter Roth, Roland Zengerle, Felix von Stetten

Source: Lab on a Chip; 12/7/2010, Vol. 10 Issue 23, p3284-3290, 7p

Subject Terms: MICROFLUIDIC devices, DNA, PHASE equilibrium, ELECTROPHORESIS, BIOMOLECULES, INTEGRATED circuits, ESCHERICHIA coli, INTERFACES (Physical sciences), LAMINAR flow

Lab-on-a-Foil: microfluidics on thin and flexible films.

Authors: Maximilian Focke, Dominique Kosse, Claas Müller, Holger Reinecke, Roland Zengerle, Felix von Stetten

Source: Lab on a Chip; 6/7/2010, Vol. 10 Issue 11, p1365-1386, 22p

Subject Terms: INTEGRATED circuits, MICROFLUIDICS, ELECTRIC properties of thin films, MICROFABRICATION, THERMISTORS, POLYMERASE chain reaction, NUCLEIC acids, IMMUNOASSAY

Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge.

Authors: Jochen Hoffmann, Daniel Mark, Sascha Lutz, Roland Zengerle, Felix von Stetten

Source: Lab on a Chip; 6/7/2010, Vol. 10 Issue 11, p1480-1484, 5p

Subject Terms: CHEMICAL reagents, DNA, EXTRACTION (Chemistry), INTEGRATED circuits, BIOLOGICAL assay, CENTRIFUGATION, MICROFLUIDIC devices, TEMPERATURE effect

Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platformsElectronic supplementary information (ESI) available: Details of measurement of surface tension and contact angles. Videos of filling of metering structure and of burst of valve. See DOI: 10.1039/b914415c

Authors: Daniel Mark, Tobias Metz, Stefan Haeberle, Sascha Lutz, Jens Ducrée, Roland Zengerle, Felix von Stetten

Source: Lab on a Chip; 12/21/2009, Vol. 9 Issue 24, p3599-3603, 5p

Subject Terms: CENTRIFUGATION, PNEUMATICS, METERING pumps, WETTING, MICROFLUIDIC devices, SURFACE tension, CONTACT angle, COMPRESSED air, GAS-liquid interfaces