An approach for vacuole isolation and vacuolar protein identification by proteomics in apple.

Authors: Xiang, Ying¹ (AUTHOR), Huang, Xiao-Yu¹ (AUTHOR), Zhao, Yu-Wen¹ (AUTHOR), Wang, Chu-Kun¹ (AUTHOR), Hu, Da-Gang¹ (AUTHOR) fap_296566@163.com

Source: New Zealand Journal of Crop & Horticultural Science. Mar2025, Vol. 53 Issue 1, p141-154. 14p.

Subject Terms: *PROTEOMICS, *DENSITY gradient centrifugation, *PLANT organelles, *ORGANELLES, *ENERGY storage

Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis.

Authors: Sun, Lu¹ (AUTHOR) sunlu9@mail2.sysu.edu.cn, Huang, Jiayu^1,2 (AUTHOR) huangjy289@mail2.sysu.edu.cn, Wang, Xuezi³ (AUTHOR) wangxz8@mail2.sysu.edu.cn, Huang, Peng⁴ (AUTHOR) huangp93@mail2.sysu.edu.cn, Dong, Baolin³ (AUTHOR) dongblin@mail2.sysu.edu.cn, Liang, Zehang³ (AUTHOR) liangzh73@mail2.sysu.edu.cn, Wu, Jiahong³ (AUTHOR) wujh268@mail2.sysu.edu.cn, Wang, Jiancheng¹ (AUTHOR) wangjch38@mail.sysu.edu.cn

Source: Reproductive Biology & Endocrinology. 2/6/2025, Vol. 23 Issue 1, p1-15. 15p.

Subject Terms: *CELL enucleation, *DENSITY gradient centrifugation, *MEDICAL sciences, *CELL physiology, *CYTOLOGY, *SPERMATOGENESIS

Analysis of kinetic parameters of sexed Holstein-Friesian bull spermatozoa using Percoll density gradient centrifugation with computer-assisted sperm analysis.

Authors: Utami, Putri¹, Anugra Yekti, Aulia Puspita¹, Aperi Simbolon, Chairun Nisa¹, Syah, Habib Asshidiq¹, Amaliya, Anny², Siswoyo, Tri Agus³, Isnaini, Nurul¹, Susilawati, Trinil¹ tsusilawati@ub.ac.id

Source: Veterinary World. Feb2025, Vol. 18 Issue 2, p287-295. 9p.

Subject Terms: *DENSITY gradient centrifugation, *SEXING of animals, *ARTIFICIAL insemination, *ONE-way analysis of variance, *DAIRY cattle

Expression of Concern: Sialidase NEU3 Dynamically Associates to Different Membrane Domains Specifically Modifying Their Ganglioside Pattern and Triggering Akt Phosphorylation.

Source: PLoS ONE. 1/15/2025, Vol. 20 Issue 1, p1-4. 4p.

Subject Terms: *DENSITY gradient centrifugation, *TRITON X-100, *OPACITY (Optics), *WESTERN immunoblotting, *RADIOACTIVITY, *NEURAMINIDASE

The Association of Monocyte Subtypes Frequency and Serum TNF-α and TGF-β Levels with Diabetic Wound Grade.

Authors: Asghariazar, Vahid¹, Dolatkhah, Roya², Moharami, Sepideh¹, Iranpour, Sohrab³, Adli, Amirhossein², Eterafi, Majid¹, Safarzadeh, Elham^1,4 Elham.im63@gmail.com

Source: Iranian Journal of Allergy, Asthma & Immunology. Apr2025, Vol. 24 Issue 2, p170-179. 10p.

Subject Terms: *MONONUCLEAR leukocytes, *DENSITY gradient centrifugation, *TYPE 2 diabetes, *ENZYME-linked immunosorbent assay, *GLYCEMIC control

An improvised one-step OptiPrep cushion ultracentrifugation method for outer membrane vesicles isolation of Klebsiella pneumoniae.

Authors: Li, Ping^1,2 (AUTHOR), Lu, Ming^1,2 (AUTHOR), Peng, Tingxiu¹ (AUTHOR), Wu, Yifan¹ (AUTHOR), Zhu, Lanlan^1,2 (AUTHOR), Liu, Yang^2,3 (AUTHOR), Zhang, Wei^1,2 (AUTHOR) zhangweiliuxin@163.com, Xiang, Tianxin^2,4 (AUTHOR) ndyfy02258@ncu.edu.cn

Source: BMC Microbiology. 12/28/2024, Vol. 24 Issue 1, p1-12. 12p.

Subject Terms: *EXTRACELLULAR vesicles, *DENSITY gradient centrifugation, *PARTICLE size distribution, *KLEBSIELLA pneumoniae, *BIOLOGICAL assay, *QUORUM sensing

Enhanced sperm isolation via bulk acoustic waves for high-throughput motility screening.

Authors: Saeidpour, Zahra¹ (AUTHOR), Bouloorchi Tabalvandani, Mohammadjavad¹ (AUTHOR), Javadizadeh, Saeed¹ (AUTHOR), Badieirostami, Majid¹ (AUTHOR) mbadiei@ut.ac.ir

Source: Scientific Reports. 11/4/2024, Vol. 14 Issue 1, p1-11. 11p.

Subject Terms: *DENSITY gradient centrifugation, *SOUND waves, *SPERM motility, *HIGH throughput screening (Drug development), *SPERMATOZOA, *FROZEN semen

Transcriptomic analysis of spleen-derived macrophages in response to lipopolysaccharide shows dependency on the MyD88-independent pathway in Chinese giant salamanders (Andrias davidianus).

Authors: Deng, Jie¹ (AUTHOR), Han, Mengdi² (AUTHOR), Gong, Jingyu² (AUTHOR), Ma, Hongying¹ (AUTHOR), Hao, Yinting² (AUTHOR), Fang, Cheng¹ (AUTHOR), Zhang, Han¹ (AUTHOR), Li, Jia² (AUTHOR), Jiang, Wei¹ (AUTHOR) jiangwei197981@ms.xab.ac.cn

Source: BMC Genomics. 10/28/2024, Vol. 25 Issue 1, p1-12. 12p.

Subject Terms: *DENSITY gradient centrifugation, *TOLL-like receptors, *GRAM-negative bacteria, *ADAPTOR proteins, *MACROPHAGE activation, *TRANSFORMING growth factors, *MYELOID differentiation factor 88

Investigation of optimal sperm storage conditions for short-term storage.

Authors: Özcan, Aykut¹ aykut.zcn@hotmail.com, Tulay, Pınar^2,3,4 pinar.tulay@neu.edu.tr, İrez, Tülay¹ tulay.irez@yeniyuzyil.edu.tr

Source: Bangladesh Journal of Medical Science. Oct2024, Vol. 23 Issue 4, p1137-1141. 5p.

Subject Terms: *DENSITY gradient centrifugation, *SEMEN analysis, *REPRODUCTIVE technology, *ACRIDINE orange, *PROPIDIUM iodide, *CENTRIFUGATION

Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes.

Authors: Serrano-Albal, Maria¹ (AUTHOR), Aquilina, Marie Claire¹ (AUTHOR) mca25@kent.ac.uk, Kiazim, Lucas G.¹ (AUTHOR), Zak, Louisa J.² (AUTHOR), Griffin, Darren K.¹ (AUTHOR), Ellis, Peter J.¹ (AUTHOR)

Source: Animals (2076-2615). Sep2024, Vol. 14 Issue 17, p2544. 15p.

Subject Terms: *DENSITY gradient centrifugation, *FERTILIZATION in vitro, *SPERM motility, *REPRODUCTIVE health, *SEMEN, *SPERMATOZOA

Density Gradient Centrifugation Is an Effective Tool to Isolate Cancer Stem-like Cells from Hypoxic and Normoxia Triple-Negative Breast Cancer Models.

Authors: Sargiacomo, Camillo¹ (AUTHOR) c.sargiacomo@salford.ac.uk, Klepinin, Aleksandr^1,2 (AUTHOR) aleksandr.klepinin@kbfi.ee

Source: International Journal of Molecular Sciences. Aug2024, Vol. 25 Issue 16, p8958. 17p.

Subject Terms: *TRIPLE-negative breast cancer, *DENSITY gradient centrifugation, *CANCER stem cells, *GENETIC overexpression, *BREAST cancer

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach †.

Authors: Wendlandt, Tim¹ (AUTHOR) tim.wendlandt@bio.uni-stuttgart.de, Britz, Beate¹ (AUTHOR) beate.britz@mpa.uni-stuttgart.de, Kleinow, Tatjana¹ (AUTHOR) tatjana.kleinow@bio.uni-stuttgart.de, Hipp, Katharina² (AUTHOR) katharina.hipp@tuebingen.mpg.de, Eber, Fabian J.³ (AUTHOR) fabian.eber@hs-offenburg.de, Wege, Christina¹ (AUTHOR) christina.wege@bio.uni-stuttgart.de

Source: Viruses (1999-4915). Jun2024, Vol. 16 Issue 6, p884. 42p.

Subject Terms: *MOSAIC diseases, *DENSITY gradient centrifugation, *PLANT diseases, *POLYETHYLENE glycol, *TOBACCO mosaic virus

Peroxisomal Localization of a Truncated HMG-CoA Reductase under Low Cholesterol Conditions.

Authors: Wang, Jianqiu¹ (AUTHOR), Kunze, Markus¹ (AUTHOR), Villoria-González, Andrea¹ (AUTHOR), Weinhofer, Isabelle¹ (AUTHOR), Berger, Johannes¹ (AUTHOR) johannes.berger@meduniwien.ac.at

Source: Biomolecules (2218-273X). Feb2024, Vol. 14 Issue 2, p244. 17p.

Subject Terms: *DENSITY gradient centrifugation, *WESTERN immunoblotting, *EXTRACELLULAR matrix proteins, *STATINS (Cardiovascular agents), *MEMBRANE proteins

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing.

Authors: Amano, Kenji¹ (AUTHOR), Oigawa, Satoko¹ (AUTHOR), Ichizawa, Koichiro¹ (AUTHOR), Tokuda, Yukiko¹ (AUTHOR), Unagami, Mami¹ (AUTHOR), Sekiguchi, Mami^1,2 (AUTHOR), Furui, Mayuko^1,2 (AUTHOR), Nakaoka, Kentaro^1,2 (AUTHOR), Ito, Ayumu^1,2 (AUTHOR), Hayashi, Rika^1,2 (AUTHOR), Tamaki, Yuko^1,2 (AUTHOR), Hayashi, Yuko^1,2 (AUTHOR), Fukuda, Yusuke^1,2 (AUTHOR), Katagiri, Yukiko^1,2 (AUTHOR) yukikonk@med.toho‐u.ac.jp, Nakata, Masahiko² (AUTHOR), Nagao, Koichi^1,3 (AUTHOR)

Source: Reproductive Medicine & Biology. Jan2024, Vol. 23 Issue 1, p1-6. 6p.

Subject Terms: *DENSITY gradient centrifugation, *SPERM motility, *SPERMATOZOA, *CENTRIFUGATION, *DNA

A methodological primer of extracellular vesicles isolation and characterization via different techniques.

Authors: Aliakbari, Farhang¹ (AUTHOR), Stocek, Noah B² (AUTHOR), Cole-André, Maxximuss² (AUTHOR), Gomes, Janice¹ (AUTHOR), Fanchini, Giovanni^2,3 (AUTHOR), Pasternak, Stephen H^1,4 (AUTHOR), Christiansen, Gunna⁵ (AUTHOR), Morshedi, Dina⁶ (AUTHOR), Volkening, Kathryn^1,4 (AUTHOR), Strong, Michael J^1,4 (AUTHOR)

Source: Biology Methods & Protocols. 2024, Vol. 9 Issue 1, p1-16. 16p.

Subject Terms: *DENSITY gradient centrifugation, *EXTRACELLULAR vesicles, *CELL separation, *ULTRACENTRIFUGATION, *SALT

A Narrative Review on the Sperm Selection Methods in Assisted Reproductive Technology: Out with the New, the Old Is Better?

Authors: Tiptiri-Kourpeti, Angeliki¹ (AUTHOR) mbg_tiptiri@yahoo.gr, Asimakopoulos, Byron² (AUTHOR) basima@med.duth.gr, Nikolettos, Nikolaos³ (AUTHOR) nnikolet@med.duth.gr

Source: Journal of Clinical Medicine. Feb2025, Vol. 14 Issue 4, p1066. 27p.

Subject Terms: *DENSITY gradient centrifugation, *REPRODUCTIVE technology, *SEMEN analysis, *TECHNOLOGICAL innovations, *MALE infertility

Embryology outcomes of a device-based sperm separation technique compared to density gradient centrifugation using thawed spermatozoa—a sibling donor oocyte study.

Authors: Gavriil, Eleftherios¹ (AUTHOR) egavriil@newlife-ivf.gr, Desli, Anastasia¹ (AUTHOR), Geladaris, Vasileios¹ (AUTHOR), Kachpani, Elli¹ (AUTHOR), Neofytou, Eirini¹ (AUTHOR), Tatsi, Petroula¹ (AUTHOR), Dovas, Dimitrios² (AUTHOR)

Source: Journal of Assisted Reproduction & Genetics. Jan2025, Vol. 42 Issue 1, p97-105. 9p.

Subject Terms: *DENSITY gradient centrifugation, *OVUM donation, *OVUM, *INTRACYTOPLASMIC sperm injection, *EMBRYOLOGY, *SPERMATOZOA

Label-Free CD34+ Cell Identification Using Deep Learning and Lens-Free Shadow Imaging Technology.

Authors: Baik, Minyoung¹, als5dud@korea.ac.kr, Shin, Sanghoon¹, ghost10s@korea.ac.kr, Kumar, Samir¹, skumar@korea.ac.kr, Seo, Dongmin², dseo@semyung.ac.kr, Lee, Inha³, dlsgk1017@korea.ac.kr, Jun, Hyun Sik³, toddjun@korea.ac.kr, Kang, Ka-Won⁴, ggm1018@korea.ac.kr, Kim, Byung Soo⁴, kbs0309@korea.ac.kr, Nam, Myung-Hyun⁵, yuret@korea.ac.kr, Seo, Sungkyu¹, yuret@korea.ac.kr

Source: Biosensors (2079-6374); Dec2023, Vol. 13 Issue 12, p993, 15p

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation.

Authors: Anunciado-Koza, Rea Victoria P.¹ (AUTHOR), Guntur, Anyonya R.^1,2,3 (AUTHOR), Vary, Calvin P.^1,2,3 (AUTHOR), Gartner, Carlos A.¹ (AUTHOR), Nowak, Madeleine^1,2 (AUTHOR), Koza, Robert A.^1,2,4 (AUTHOR) Robert.Koza@mainehealth.org

Source: BMC Research Notes. 11/18/2023, Vol. 16 Issue 1, p1-7. 7p.

Subject Terms: *DENSITY gradient centrifugation, *MITOCHONDRIA, *PLANT mitochondria, *MITOCHONDRIAL proteins, *BIOENERGETICS, *MICE

Spermatozoa isolation with Felix™ outperforms conventional density gradient centrifugation preparation in selecting cells with low DNA damage.

Authors: Villeneuve, Pauline¹ (AUTHOR), Saez, Fabrice¹ (AUTHOR), Hug, Elisa¹ (AUTHOR), Chorfa, Areski¹ (AUTHOR), Guiton, Rachel¹ (AUTHOR), Schubert, Benoit² (AUTHOR), Force, André² (AUTHOR) andreforce@eurofins-biomnis.com, Drevet, Joël R.¹ (AUTHOR) joel.drevet@uca.fr

Source: Andrology. Nov2023, Vol. 11 Issue 8, p1593-1604. 12p.

Subject Terms: *DENSITY gradient centrifugation, *HUMAN artificial insemination, *SPERMATOZOA, *DNA damage, *REPRODUCTIVE technology, *SEMEN

Geographic Terms: SYDNEY (N.S.W.)