Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

Authors: Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost

Source: eLife, Vol 11 (2022)

Subject Terms: CRISPR, CRISPR interference, genetic screen, functional genomics, knockdown, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/81856; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/af03cd98b4f34c128b98cd963fa2ba56

Endoplasmic reticulum tubules limit the size of misfolded protein condensates

Authors: Smriti Parashar, Ravi Chidambaram, Shuliang Chen, Christina R Liem, Eric Griffis, Gerard G Lambert, Nathan C Shaner, Matthew Wortham, Jesse C Hay, Susan Ferro-Novick

Source: eLife, Vol 10 (2021)

Subject Terms: ER-phagy, protein quality control, misfolded prohormones, SEC24C, Lunapark, ER structure, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/71642; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/bfdc35449f78485fab17dbdda6923c5b

Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether

Authors: Sandra Elizabeth Torres, Ciara M Gallagher, Lars Plate, Meghna Gupta, Christina R Liem, Xiaoyan Guo, Ruilin Tian, Robert M Stroud, Martin Kampmann, Jonathan S Weissman, Peter Walter

Source: eLife, Vol 8 (2019)

Subject Terms: unfolded protein response, proteostasis, small molecule inhibition, Medicine, Science, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://elifesciences.org/articles/46595; https://doaj.org/toc/2050-084X

Access URL: https://doaj.org/article/1385a79477e045e0a8efc889a4a6ac9d