The metal cofactor: stationary or mobile?

Authors: Hagedoorn, Peter-Leon¹ (AUTHOR), Pabst, Martin¹ (AUTHOR), Hanefeld, Ulf¹ (AUTHOR) u.hanefeld@tudelft.nl

Source: Applied Microbiology & Biotechnology. 6/24/2024, Vol. 108 Issue 1, p1-15. 15p.

Subject Terms: *COFACTORS (Biochemistry), *METALS, *ALDOLASES, *BIOCHEMICAL substrates, *ISOMERASES, *METAL ions

Biochemical and Structural Characterization of Glyoxylate Reductase/Hydroxypyruvate Reductase from Bacillus subtilis.

Authors: Nguyen, Thang Quyet^1,2, Duong, Thai Huu², Yang, Jin Kuk^1,3, Kang, Wonchull^1,3, wonchullkang@ssu.ac.kr

Source: Crystals (2073-4352); Apr2025, Vol. 15 Issue 4, p298, 14p

Loop 6 and the β‐hairpin flap are structural hotspots that determine cofactor specificity in the FMN‐dependent family of ene‐reductases.

Authors: Kerschbaumer, Bianca¹ (AUTHOR), Totaro, Massimo G.¹ (AUTHOR), Friess, Michael² (AUTHOR), Breinbauer, Rolf² (AUTHOR), Bijelic, Aleksandar¹ (AUTHOR) aleksandar.bijelic@tugraz.at, Macheroux, Peter¹ (AUTHOR) peter.macheroux@tugraz.at

Source: FEBS Journal. Apr2024, Vol. 291 Issue 7, p1560-1574. 15p.

Subject Terms: *COFACTORS (Biochemistry), *FLAVIN mononucleotide, *MOLECULAR dynamics, *CARBON-carbon bonds, *TOMATOES, *COENZYMES, *OXIDOREDUCTASES, *NICOTINAMIDE

On the Shoulders of Giants—Reaching for Nitrogenase.

Authors: Einsle, Oliver¹ (AUTHOR) einsle@biochemie.uni-freiburg.de

Source: Molecules. Dec2023, Vol. 28 Issue 24, p7959. 21p.

Subject Terms: *NITROGENASES, *COFACTORS (Biochemistry), *NITROGEN fixation, *ATMOSPHERIC oxygen, *ENZYMES, *TAPESTRY

Nitrogenase beyond the Resting State: A Structural Perspective.

Authors: Warmack, Rebeccah A.^1,2 (AUTHOR) rwarmack@caltech.edu, Rees, Douglas C.^1,2 (AUTHOR) rwarmack@caltech.edu

Source: Molecules. Dec2023, Vol. 28 Issue 24, p7952. 25p.

Subject Terms: *NITROGENASES, *COFACTORS (Biochemistry), *IRON clusters, *CHARGE exchange, *PROTON transfer reactions, *NITROGEN fixation, *HYDRIDES

A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis.

Authors: Gao, Hui¹ (AUTHOR), Li, Mengxuan¹ (AUTHOR), Wang, Qing¹ (AUTHOR), Liu, Tingting² (AUTHOR), Zhang, Xian¹ (AUTHOR), Yang, Taowei¹ (AUTHOR), Xu, Meijuan¹ (AUTHOR) xumeijuan@jiangnan.edu.cn, Rao, Zhiming¹ (AUTHOR)

Source: Biotechnology for Biofuels & Bioproducts. 9/14/2023, Vol. 16 Issue 1, p1-13. 13p.

Subject Terms: *BIOCATALYSIS, *COFACTORS (Biochemistry), *HIGH throughput screening (Drug development), *CATALYTIC activity, *VALUE (Economics), *GLUTAMINE, *GLUTAMIC acid

NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria.

Authors: Kushkevych, Ivan¹ (AUTHOR) ivan.kushkevych@gmail.com, Dordević, Dani² (AUTHOR), Alberfkani, Mohammad I.³ (AUTHOR), Gajdács, Márió⁴ (AUTHOR), Ostorházi, Eszter⁵ (AUTHOR), Vítězová, Monika¹ (AUTHOR), Rittmann, Simon K.-M. R.⁶ (AUTHOR) simon.rittmann@univie.ac.at

Source: Scientific Reports. 8/25/2023, Vol. 13 Issue 1, p1-11. 11p.

Subject Terms: *SULFATE-reducing bacteria, *COFACTORS (Biochemistry), *PEROXIDASE, *NICOTINAMIDE adenine dinucleotide phosphate, *INTESTINES, *ANIMAL droppings, *ENZYME kinetics

Kinetic Characterization and Catalytic Mechanism of N -Acetylornithine Aminotransferase Encoded by slr1022 Gene from Synechocystis sp. PCC6803.

Authors: Li, Zhi-Min¹ (AUTHOR), Bai, Fumei² (AUTHOR), Wang, Xiaoqin² (AUTHOR), Xie, Congcong² (AUTHOR), Wan, Yuting² (AUTHOR), Li, Yating² (AUTHOR), Liu, Jianping^2,3 (AUTHOR) jianpingliu@jxau.edu.cn, Li, Zhimin^2,3 (AUTHOR) jianpingliu@jxau.edu.cn

Source: International Journal of Molecular Sciences. Mar2023, Vol. 24 Issue 6, p5853. 16p.

Subject Terms: *SYNECHOCYSTIS, *COFACTORS (Biochemistry), *AMINO acids, *ORNITHINE, *GENES, *ARGININE

Quick and Spontaneous Transformation between [3Fe–4S] and [4Fe–4S] Iron–Sulfur Clusters in the tRNA-Thiolation Enzyme TtuA.

Authors: Ishizaka, Masato¹ (AUTHOR), Chen, Minghao² (AUTHOR), Narai, Shun¹ (AUTHOR), Tanaka, Yoshikazu^2,3 (AUTHOR), Ose, Toyoyuki^1,2 (AUTHOR), Horitani, Masaki^4,5 (AUTHOR) horitani@cc.saga-u.ac.jp, Yao, Min^1,2 (AUTHOR) horitani@cc.saga-u.ac.jp

Source: International Journal of Molecular Sciences. Jan2023, Vol. 24 Issue 1, p833. 13p.

Subject Terms: *COFACTORS (Biochemistry), *ELECTRON paramagnetic resonance, *ENZYMES, *CATALYTIC activity

The Effects of Nε-Acetylation on The Enzymatic Activity of Escherichia coli Glyceraldehyde-3-Phosphate Dehydrogenase.

Authors: Plekhanova, N. S.¹, plekhanovans@mail.ru, Altman, I. B.², Yurkova, M. S.¹, Fedorov, A. N.¹

Source: Applied Biochemistry & Microbiology; Dec2023, Vol. 59 Issue 6, p778-785, 8p

Structural Comparison of hMDH2 Complexed with Natural Substrates and Cofactors: The Importance of Phosphate Binding for Active Conformation and Catalysis.

Authors: Eo, Yumi¹ (AUTHOR), Duong, Men Thi Hoai¹ (AUTHOR), Ahn, Hee-Chul¹ (AUTHOR) hcahn@dongguk.edu

Source: Biomolecules (2218-273X). Sep2022, Vol. 12 Issue 9, p1175-1175. 14p.

Subject Terms: *COFACTORS (Biochemistry), *MALATE dehydrogenase, *KREBS cycle, *X-ray crystallography, *CATALYSIS, *ISOTHERMAL titration calorimetry

Rational Design of a Calcium-Independent Trypsin Variant.

Authors: Simon, Andreas H.¹ (AUTHOR), Liebscher, Sandra¹ (AUTHOR), Kattner, Ariunkhur¹ (AUTHOR), Kattner, Christof¹ (AUTHOR), Bordusa, Frank¹ (AUTHOR) frank.bordusa@biochemtech.uni-halle.de

Source: Catalysts (2073-4344). Sep2022, Vol. 12 Issue 9, pN.PAG-N.PAG. 9p.

Subject Terms: *COFACTORS (Biochemistry), *CALCIUM ions, *TRYPSIN, *ENZYME kinetics, *PROTEIN folding, *CATALYTIC activity, *CRYSTAL structure

Redox Active α-Lipoic Acid Differentially Improves Mitochondrial Dysfunction in a Cellular Model of Alzheimer and Its Control Cells.

Authors: Dieter, Fabian¹ (AUTHOR), Esselun, Carsten¹ (AUTHOR), Eckert, Gunter P.¹ (AUTHOR) eckert@uni-giessen.de

Source: International Journal of Molecular Sciences. Aug2022, Vol. 23 Issue 16, p9186-N.PAG. 14p.

Subject Terms: *COFACTORS (Biochemistry), *MITOCHONDRIA, *CITRATE synthase, *OCTANOIC acid, *CYTOCHROME oxidase, *LIPOIC acid

A phosphite-based screening platform for identification of enzymes favoring nonnatural cofactors.

Authors: Liu, Yuxue^1,2 (AUTHOR) liuyuxue@htu.edu.cn, Li, Zhuoya¹ (AUTHOR), Guo, Xiaojia² (AUTHOR), Wang, Xueying² (AUTHOR), Zhao, Zongbao K.² (AUTHOR)

Source: Scientific Reports. 7/21/2022, Vol. 12 Issue 1, p1-8. 8p.

Subject Terms: *COFACTORS (Biochemistry), *ENZYMES, *NICOTINAMIDE, *SYNTHETIC biology, *CHEMICAL biology, *OXIDATION-reduction reaction, *BIOCATALYSIS

Construction and characterization of BsGDH-CatIB variants and application as robust and highly active redox cofactor regeneration module for biocatalysis.

Authors: Küsters, Kira^1,2 (AUTHOR), Saborowski, Ronja¹ (AUTHOR), Wagner, Christian¹ (AUTHOR), Hamel, Rebecca¹ (AUTHOR), Spöring, Jan-Dirk^1,3 (AUTHOR), Wiechert, Wolfgang^1,4 (AUTHOR), Oldiges, Marco^1,2 (AUTHOR) m.oldiges@fz-juelich.de

Source: Microbial Cell Factories. 6/2/2022, Vol. 21 Issue 1, p1-10. 10p.

Subject Terms: *COFACTORS (Biochemistry), *BIOCATALYSIS, *IMMOBILIZED enzymes, *CELLULAR inclusions, *GLYCOGENOLYSIS, *BACILLUS subtilis

Disruption of Redox Homeostasis by Alterations in Nitric Oxide Synthase Activity and Tetrahydrobiopterin along with Melanoma Progression.

Authors: Soares, Jaqueline Pereira Moura¹ (AUTHOR) jpms1995@outlook.com, Gonçalves, Diego Assis^2,3 (AUTHOR) diegoassisg@gmail.com, de Sousa, Ricardo Xisto¹ (AUTHOR) xisto1987@hotmail.com, Mouro, Margareth Gori⁴ (AUTHOR) margaret_gori@hotmail.com, Higa, Elisa M. S.⁴ (AUTHOR) emshiga@gmail.com, Sperandio, Letícia Paulino⁵ (AUTHOR) leticiasperandio7@gmail.com, Vitoriano, Carolina Moraes⁶ (AUTHOR) car.vitoriano@gmail.com, Rosa, Elisa Bachir Santa⁶ (AUTHOR) elisa.bsr@hotmail.com, Santos, Fernanda Oliveira dos⁶ (AUTHOR) fernanda.sts00@outlook.com, de Queiroz, Gustavo Nery⁶ (AUTHOR) guhnery0@gmail.com, Yamaguchi, Roberta Sessa Stilhano¹ (AUTHOR) robertasessa@gmail.com, Pereira, Gustavo⁵ (AUTHOR) gustavo.pereira@unifesp.br, Icimoto, Marcelo Yudi⁷ (AUTHOR) icimoto@unifesp.br, Melo, Fabiana Henriques Machado de^6,8 (AUTHOR) fabihmmelo2020@gmail.com

Source: International Journal of Molecular Sciences. Jun2022, Vol. 23 Issue 11, p5979-5979. 20p.

Subject Terms: *NITRIC-oxide synthases, *COFACTORS (Biochemistry), *TETRAHYDROBIOPTERIN, *MICROPHTHALMIA-associated transcription factor, *WESTERN immunoblotting, *HOMEOSTASIS, *REACTIVE oxygen species

Magnesium induced structural reorganization in the active site of adenylate kinase.

Authors: Kwangho Nam¹ kwangho.nam@uta.edu, Arattu Thodika, Abdul Raafik¹, Tischlik, Sonja², Phoeurk, Chanrith^3,4, Nagy, Tamás Milán³, Schierholz, Léon^3,5, Ådén, Jörgen³, Rogne, Per³, Drescher, Malte², Sauer-Eriksson, A. Elisabeth³ elisabeth.sauer-eriksson@umu.se, Wolf-Watz, Magnus³ magnus.wolf-watz@umu.se

Source: Science Advances. 8/9/2024, Vol. 10 Issue 32, p1-13. 13p.

Subject Terms: *COFACTORS (Biochemistry), *BINDING sites, *ENZYME kinetics, *ENZYME activation, *MAGNESIUM, *CELL communication

Utilizing Hairy Hollow Conjugated Microporous Polymers and Native Enzymes for Precise Aerobic Photocatalysis Under Near‐Infrared Wavelengths.

Authors: Xiang, Qian^1,2 (AUTHOR), Huang, Yuan^1,2 (AUTHOR), Jiang, Sheng Hong^1,2 (AUTHOR), Cheng, Si‐Xue¹ (AUTHOR), Li, Xue^1,2 (AUTHOR) li.x@whu.edu.cn, Cai, Tao^1,2 (AUTHOR) chemcaitao@whu.edu.cn

Source: Advanced Functional Materials. 7/17/2024, Vol. 34 Issue 29, p1-9. 9p.

Subject Terms: *COFACTORS (Biochemistry), *CONJUGATED polymers, *POLYMERS, *NEAR infrared radiation, *REACTIVE oxygen species, *PHOTOCATALYSIS, *ENZYMES

Metal‐semiconductor heterojunction accelerates the plasmonically powered photoregeneration of biological cofactors.

Authors: Deepak, Namitha¹ (AUTHOR), Jain, Vanshika¹ (AUTHOR) jain.vanshika@students.iiserpune.ac.in, Pillai, Pramod P.¹ (AUTHOR) pramod.pillai@iiserpune.ac.in

Source: Photochemistry & Photobiology. Jul2024, Vol. 100 Issue 4, p1000-1009. 10p.

Subject Terms: *COFACTORS (Biochemistry), *HETEROJUNCTIONS, *CHEMICAL amplification, *CHEMICAL bonds, *SCHOTTKY barrier, *N-type semiconductors, *PHOTOCATALYSTS

Novel Pt/CdS Schottky Junction Catalysts for Efficient and Stable Photocatalytic NADH Regeneration Through Optimized Carrier Migration and Free Radical Generation.

Authors: Su, Zheng^1,2 (AUTHOR), Sun, Yu^1,2 (AUTHOR), Zhang, Qi^1,2 (AUTHOR), Wang, Jia^1,2 (AUTHOR), Zhou, Wenqi^1,2 (AUTHOR), Zhou, Suyin^1,2 (AUTHOR), Zhang, Jinlong^1,2,3 (AUTHOR), Liu, Yongdi^1,2 (AUTHOR), Zhou, Liang^1,2,4 (AUTHOR) zhouliang@ecust.edu.cn, Lei, Juying^1,2,4 (AUTHOR)

Source: Catalysis Letters. Jun2024, Vol. 154 Issue 6, p2620-2632. 13p.

Subject Terms: *FREE radicals, *COFACTORS (Biochemistry), *HYDROXYL group, *METAL catalysts, *CONDUCTION bands, *CATALYSTS, *REACTIVE oxygen species